Polyphenols are known dietary antioxidants. They have recently attracted considerable interest in uses to prevent skin aging and hyperpigmentation resulting from solar UV-irradiation. Prunus persica (L.) leaves are considered by-products and were reported to have a remarkable antioxidant activity due to their high content of polyphenols. This study aimed at the development of a cosmeceutical anti-aging and skin whitening cream preparation using ethanol leaves extract of Prunus persica (L.) (PPEE) loaded in solid lipid nanoparticles (SLNs) to enhance the skin delivery. Chemical investigation of PPEE showed significantly high total phenolic and flavonoids content with notable antioxidant activities (DPPH, ABTS, and β-carotene assays). A unique acylated kaempferol glycoside with a rare structure, kaempferol 3-O-β-⁴C₁-(6″-O-3,4-dihydroxyphenylacetyl glucopyranoside) (KDPAG) was isolated for the first time and its structure fully elucidated. It represents the first example of acylation with 3,4-dihydroxyphenyl acetic acid in flavonoid chemistry. The in-vitro cytotoxicity studies against a human keratinocytes cell line revealed the non-toxicity of PPEE and PPEE-SLNs. Moreover, PPEE, PPEE-SLNs, and KDPAG showed good anti-elastase activity, comparable to that of N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone. Besides, PPEE-SLNs and KDPAG showed significantly (p < 0.001) higher anti-collagenase and anti-tyrosinase activities in comparison to EDTA and kojic acid, respectively. Different PPEE-SLNs cream formulae (2% and 5%) were evaluated for possible anti-wrinkle activity against UV-induced photoaging in a mouse model using a wrinkle scoring method and were shown to offer a highly significant protective effect against UV, as evidenced by tissue biomarkers (SOD) and histopathological studies. Thus, the current study demonstrates that Prunus persica leaf by-products provide an interesting, valuable resource for natural cosmetic ingredients. This provides related data for further studying the potential safe use of PPEE-SLNs in topical anti-aging cosmetic formulations with enhanced skin permeation properties.

A crucial target in drug research is magnifying efficacy and decreasing toxicity. Therefore, using natural active constituents as precursors will enhance both safety and biological activities. Despite having many pharmacological activities, caffeic and ferulic acids showed limited clinical usage due to their poor bioavailability and fast elimination. Therefore, semisynthetic compounds from these two acids were prepared and screened as anticancer agents. In this study, CA and FA showed very potent anticancer activity against Caco-2 cells. Consequently, eighteen derivatives were tested against the same cell line. Four potent candidates were selected for determination of the selectivity index, where compound 10 revealed a high safety margin. Compound 10 represented a new scaffold and showed significant cytotoxic activity against Caco-2. Cell-cycle analysis and evaluation of apoptosis showed that derivatives 10, 7, 11, 15 and 14 showed the highest proportion of cells in a late apoptotic stage.

Introduction: Liver fibrosis is currently the 11th most common cause of death worldwide. Because of self-renewal, available sources for isolation, and high differentiation properties, multipotent mesenchymal stromal stem cells are suggested to be potential tool for treatment of liver fibrosis....

Oxidative stress and neuroinflammation are key components in neurodegenerative diseases, where early intervention using natural treatments may offer neuroprotective effects. This study shows that isoliquiritigenin (ISL), hesperidin (HES), and curcumin (CUR) can mitigate lipopolysaccharide (LPS)‐induced neuroinflammation and oxidative stress in vitro and in vivo. The compounds were initially tested for cytotoxicity and found to reduce nitric oxide (NO) production, especially CUR and ISL. They were able to restore antioxidant enzyme activities both in vivo and in vitro. All treatments reduced inducible nitric oxide synthase (iNOS) expression compared to the untreated LPS control group. Behavioral assessments indicated that LPS impaired spatial and nonspatial memory, but treatments improved cognitive performance. Biomarker analyses revealed that ISL, HES, and CUR reduced the interleukin (IL)‐1β and nuclear factor erythroid 2‐related factor 2 (Nrf2) ratio in the hippocampus. Moreover, they decreased the level of caspase‐3 demonstrated by western blotting and tumor necrosis factor‐α (TNF‐α) level. Thereby they inhibited LPS elicited apoptosis. Likewise, their anti‐inflammatory effects were illustrated in the histopathological examination. Furthermore, they decreased the expression of amyloid‐β. The study reinforces the potential of these natural compounds as protective and therapeutic cost‐effective alternatives for managing neuroinflammation and neurodegeneration.