There is an increasing interest in polyphenols, plant secondary metabolites, in terms of fruit quality and diet, mainly due to their antioxidant effect. However, the identification of key gene enzymes and their roles in the phenylpropanoid pathway in temperate fruits species remains uncertain. Apricot (Prunus armeniaca) is a Mediterranean fruit with high diversity and fruit quality properties, being an excellent source of polyphenol compounds. For a better understanding of the phenolic pathway in these fruits, we selected a set of accessions with genetic-based differences in phenolic compounds accumulation. HPLC analysis of the main phenolic compounds and transcriptional analysis of the genes involved in key steps of the polyphenol network were carried out. Phenylalanine ammonia-lyase (PAL), dihydroflavonol-4-reductase (DFR) and flavonol synthase (FLS) were the key enzymes selected. Orthologous of the genes involved in transcription of these enzymes were identified in apricot: ParPAL1, ParPAL2, ParDFR, ParFLS1 and ParFLS2. Transcriptional data of the genes involved in those critical points and their relationships with the polyphenol compounds were analyzed. Higher expression of ParDFR and ParPAL2 has been associated with red-blushed accessions. Differences in expression between paralogues could be related to the presence of a BOXCOREDCPAL cis-acting element related to the genes involved in anthocyanin synthesis ParFLS2, ParDFR and ParPAL2.

Aim of study: In a social context of increasing concern about healthy diets, the development of new varieties with enhanced content in nutraceutical compounds is an important objective of the fruit breeding programs currently developed. In this sense, apricot is a fruit crop very appreciated by consumers worldwide due to its organoleptic characteristics, but also plays an important role in human nutrition due to its content of phytocompounds as sugars, organic acids, vitamins and polyphenols. Area of study: The identification of sources of variation for these traits could be useful for apricot breeding worldwide. Material and methods: New selections from the apricot breeding program carried out at the Instituto Valenciano de Investigaciones Agrarias (IVIA, Spain) and traditional varieties have been analysed aimed at identifying sources of genetic variation for fruit quality. For this purpose, sugar content, organic acids and ascorbic acid were studied during three crop years. Main results: Results revealed sucrose and glucose as the major sugars, malic and citric acid as the main organic acids, and diverse ascorbic acid content among the cultivars studied. Research highlights: Some accessions point as potential sources to increase fruit quality. In addition, the study showed that apricot peel is an excellent source of nutraceutical compounds. Moreover, this study opens up new possibilities to study the genetic control of these traits in apricot in the future.

Apricot is one of the most important Mediterranean fruits with high diversity and fruit quality properties, being an excellent raw material for polyphenol compounds. This study aimed to determine the anthocyanin, quercetin glycoside and phenolic acid contents in new apricot genotypes from the breeding program at the Instituto Valenciano de Investigaciones Agrarias, confirming the potential of the ‘Goldrich’ cultivar to be a parental donor for increasing the antioxidant content, which would, in turn, enhance fruit quality. Phenolic composition of the apricot accessions is strongly genotype-dependent, with the concentrations of overall total phenolic compounds ranging from 770 to 260 mg 100 g⁻¹ DW, reflecting significant genetic diversity. ‘Goldrich’ contributed to the polyphenol content; however, its influence varied across derived varieties, with ‘GG9310’ and ‘GG979’ enhancing the shikimic acid pathway and accumulating high levels of total phenolics. In contrast, ‘Mitger’ and ‘HG9850’ stood out for high anthocyanin synthesis, despite their lower levels of flavonols and phenolic acids. The predominant anthocyanin was cyanidin-3-O-rutinoside, followed by cyanidin-3-O-glucoside and peonidin-3-O-rutinoside in smaller amounts. Other phenolics were rutin and quercetin-3-O-glucuronide, as well as neochlorogenic and chlorogenic acids. The PCA model was applied to all data to identify the most attractive cultivars, and chromatographic analysis was performed in a short time using Ultra-High-Performance Liquid Chromatography (UHPLC) with diode array and mass spectrometric detection. Apricot peel is an excellent source of nutraceutical compounds with a chemical composition strongly determined by the cultivar. Results can help establish authenticity markers for apricot cultivars.

Melanoma incidence is rising, and accurate risk stratification remains challenging because of the molecular heterogeneity underlying disease progression. Specifically, the transition from benign nevi to malignant melanoma and the acquisition of the aggressive ulcerated phenotype represent critical barriers in clinical management that require novel biomarkers. In addition, there is a critical need for novel biomarkers to identify patients with primary melanoma who, despite complete surgical resection, remain at high risk of recurrence and would benefit from adjuvant therapy. We applied an integrative systems biology approach to decipher the miRNA-dependent regulatory architecture of melanoma. Following PRISMA guidelines, we conducted a robust meta-analysis of six independent transcriptomic studies, overcoming inter-study heterogeneity. This was coupled with network inference algorithms to construct validated miRNA-mRNA interactomes and identify dynamic functional modules. In the diagnostic scenario, we identified a consensus signature of 24 miRNAs. Network topology analysis revealed hsa-miR-142-5p as a master regulator that orchestrates the dismantling of the Oncogene-Induced Senescence (OIS) barriers by targeting CDK6, SIRT1, and TGFBR2. In the prognostic scenario (ulceration), we identified a specific “stress-adaptive” signature of 23 miRNAs. Notably, the upregulation of hsa-miR-223-3p emerged as a key driver of invasiveness by suppressing the motility-limiting tumor suppressor RHOB, while the concurrent loss of hsa-miR-200c and hsa-miR-489-3p may unleash Epithelial-Mesenchymal Transition (EMT) and EGFR-driven survival pathways. Our study supports a double-switch mechanism where specific miRNA alterations first drive senescence escape and subsequently promote survival in the hypoxic ulcerated niche. These signatures offer robust biomarkers for diagnosis and highlight the miR-142-5p/CDK6 and miR-223-3p/RHOB axes as potential therapeutic targets for precise intervention.