Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts) and biological (Trichoderma spp. isolated from the bark) components. The water bark extracts were prepared at two temperatures (80 and 120 °C) and pH regimes (7 and 9). The presence of bark extracts (30%) caused inhibition of mycelial growth of B. dothidea and D. sarmentorum for 39 to 44% and 53 to 60%, respectively. Moreover, we studied the antagonistic effect of three Trichoderma isolates originating from the pine bark. Trichoderma spp. reduced growth of B. dothidea by 67%–85%, D. sarmentorum by 63%–75% and N. parvum by 55%–62%. Microscopic examination confirmed typical mycoparasitism manifestations (coiling, parallel growth, hook-like structures). The isolates produced cellulase, β-glucosidase and N-acetyl-β-glucosaminidase. The volatile blend detected the emission of several volatile compounds with antimicrobial activity, including nonanoic acid, cubenene, cis-α-bergamotene, hexanedioic acid, and verticillol. The present study confirmed in vitro potential of P. sylvestris bark extracts and Trichoderma spp. against the Botryosphaeriaceae. The study is an important step towards the use of environmentally friendly methods of Botryosphaeriaceae disease control.

Botryosphaeriales represent a diverse order of fungal pathogens of various woody plant species. In Serbia, these fungi are important pathogens of forest, ornamental, and fruit trees causing die-back, cankers, leaf blights, fruit, and root rot. The aim of this study was to evaluate the antifungal activity of Pinus sylvestris bark extracts and Trichoderma spp. against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Ascomycota, Botryosphaeriales) isolated from Picea abies, Thuja occidentalis, and Prunus laurocerasus trees planted in urban areas in Serbia. Bark extracts were prepared in water solution at two temperatures (80 and 120 °C). The extracts were tested using two concentrations (20% and 30%). Moreover, two Trichoderma isolates obtained from P. sylvestris bark were tested against Botryosphaeriales and their antagonistic potential was estimated in vitro using a confrontation test. Mycelial growth of B. dothidea and D. sarmentorum was significantly inhibited in the presence of bark extracts, while N. parvum showed no growth inhibition. Botryosphaeria dothidea growth was inhibited by 35 to 39% in the case of 20% extracts and by 39 to 44% in the case of 30% extracts. The growth inhibition of D. sarmentorum was between 48% and 56% in the case of 20% extracts and between 53% and 60% in the case of 30% extracts. The two Trichoderma isolates showed antifungal activity against the selected pathogens. An isolate BKG 4 showed the highest inhibition level, and it inhibited the growth of B. dothidea, D. sarmentorum, and N. parvum by 85%, 75%, and 62%, respectively. Preliminary results suggest that both P. sylvestris bark extracts and Trichoderma spp. could be used as biocontrol agents against B. dothidea, D. sarmentorum, and N. parvum, and this should be a further studied.

The recommended cultivars for top quality wines Riesling in the vineyards of Grocka is in full crop. It was grafted on Kober 5 BB stock and planted on the soil type cambysoil. The content of total nitrogen is 0.1-0.15%. Supply of easily available potassium varies between 12.3-15 mg/100g a.d.s.2, i.e. phosphorus 0.4-3.6 mg/100g a.d.s. in layer up to 40 cm. Microbiological fertilizer was used in the study - biological preparation prepared with mixed natural populations Azotobacter chroococcum, Bacillus megaterium and Bacillus circulons. The space in row is idle land and the space between rows was sown each year (March-April) with a mixture of field pea and barley and ploughed in the inflorescence phase of legumes. Grape yield varied between 8772-6804 kg/ha. Microbiological fertilizer with Azotobacter had the highest yield and the control treatment had the lowest yield, where only grass mixture was sown. Extremely dry climatic conditions in the trial period caused the grape yield in cv. Riesling to be extremely low. In combination of fertilizers Bacillus megaterium + Bacillus circulons wine with the most ethanol, extracts and polyphenols was obtained. The wine obtained from the control treatment had a typical taste.

Palic and Ludas lakes are shallow Pannonian lakes, which have undergone rapid eutrophication. High concentrations of nutrients, along with significantly high values of fecal indicators in water samples, indicate that the Palic-Ludas canal, which connects them, contributes to the pollution of the recipient Lake Ludas, a central part of a special nature reserve. In order to improve water quality in the canal and decrease nutrient load of Lake Ludas, it is suggested to use environmentally friendly solutions, so-called bio-barriers (bio-bridges and biobanks), which will contribute to self-purification efficiency. The given model assumes 10 zones with bio-bridges and 0.4 ha covered by biobanks, using plants common in the area, e.g. Typha spp., Phragmites spp., Juncus spp., Scirpus spp. and Carex spp. The main disadvantage of this technology is occupation of large area; thus, the solution seems to be undersized. It removes 4% TN/year and 8% TP/year of the total amount necessary to achieve good ecological status. Nevertheless, the role of bio-bridges in permanent nutrient removal, through preventing the deposition of organic matter at the bottom sediment and later return of nutrients in water, together with indirect influence through enhancement of biodiversity should not be underestimated.