Meat is the most valuable livestock product since it is one of the main sources of protein for human consumption. Meat quality can be evaluated according to the following parameters: pH, amount of lactic acid, volatile fatty acids, bounded water, solubility of proteins, color, and tenderness. The meat composition and physical properties of muscles have been characterized for ensuring improved eating quality. Thus, the purpose of this paper was to review the major chemical compositional and physicochemical properties of meat and, at the same time, its quality attributes and factors that affect quality of meat. A number of structural features of meat as connective tissue, muscle fibers, and tendon that attaches the muscle to the bone are visible in joint meat examined through naked eyes. Water is quantitatively the most important component of meat comprising up to 75% of weight. Meat is also composed of amino acids, fatty acids, vitamins, minerals, and other important ingredients. Quality factors perceived by consumers are related to sensory attributes (e.g., color, tenderness, and flavor), nutritional properties (e.g., calories, vitamins’ content, and fatty acids’ profile), and appearance (e.g., exudation, marbling, and visible amount of fat). However, fresh meat quality can be defined instrumentally including composition, nutrients, color, water-holding capacity, tenderness, functionality, flavors, spoilage, and contamination. Visual inspection based on sensory quality attributes and different chemical methods are used to analyze meat quality. Other methods such as computer vision and imaging spectroscopy, gas chromatographic analysis, near-infrared technology, dual-energy X-ray absorptiometry, and computerized tomography scanning are also used in the meat industry. So, the aim of the present review is to review quality characteristics of cattle meat and its composition constituents.

Rotavirus is a major pathogen responsible for diarrheal disease in calves, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by rotavirus in calves in the world are inadequately understood, considering that diarrheal disease caused by rotavirus is a vital health problem in calves that interrupts production benefits with reduced weight gain and increased mortality, and its potential for zoonotic spread. The pathological changes made by rotavirus are almost exclusively limited to the small intestine that leads to diarrhea. It is environmentally distributed worldwide and was extensively studied. Reassortment is one of the important mechanisms for generating genetic diversity of rotaviruses and eventually for viral evolution. So, the primary strategy is to reduce the burden of rotavirus infections by practicing early colostrum’s feeding in newborn calves, using vaccine, and improving livestock management. Rotaviruses have a wide host range, infecting many animal species as well as humans. As it was found that certain animal rotavirus strains had antigenic similarities to some human strains, this may be an indication for an animal to play a role as a source of rotavirus infection in humans. Groups A to C have been shown to infect both humans and animals. The most commonly detected strains in both human and animals are G2, G3, G4, and G9, P [6]. Therefore, this review was made to get overview epidemiology status and zoonotic importance of bovine rotavirus.

A cross-sectional study was conducted from November 2018 to May 2019 in Bishoftu and Dukem in central part of Ethiopia. The objectives of the present study were to isolate and identify S. aureus, E. coli, and Salmonella from dairy cattle, personnel, and equipment at farms. In addition to this, antimicrobial resistance profiles of the isolates were determined. A total of 607 samples consisting of fresh cow milk (125), fecal sample (211), nasal swab (211), pooled milkers hand swabs (20), pooled floor swabs (20), and tank milk (20) samples were collected from 20 dairy farms, which included 211 animals. Structured questionnaire was designed and administered to dairy farm owners and dairy food consumers to assess their consumption behavior and antibiotics usage. The samples were examined for the presence of S. aureus, E. coli, and Salmonella following standard techniques and procedures outlined by the International Organization for Standardization. Subsequently, 62 (15.7) of S. aureus were isolated from 396 of the totals analyzed samples for S. aureus. Out of the 62 isolated S. aureus, 35/211(16.7), 19/125(15.2), 6/20(30), 2/20(10), and 0/20(0) were from nasal swabs, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. On the other hand, 30 (7.6) of E. coli were isolated from 396 of the totals analyzed samples for E. coli. Out of the 30 isolated E. coli, 17/211(8.1), 12/125(9.6), 0/20(0), 0/20(0), and 1/20(5) were from faeces, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. In line with this, 13 (4.8) of Salmonella were isolated from 271 of the totals analyzed samples for Salmonella. Out of the 13 isolated Salmonella, 10/211(4.7), 2/20(10), 0/20(0), and 1/20(5) were from faeces, bulk milk, pooled hand swab, and floor swabs, respectively. Subsequently, 62 of S. aureus, 30 of E. coli, and 13 of Salmonella isolates were subjected to antimicrobial susceptibility testing, and all isolates were resistant to at least one or more antimicrobials tested. Penicillin, methicillin, and trimethoprim/sulfamethoxazole are drugs to which a large proportion of isolated S. aureus were highly resistant, which range from 90 to 100. From 30 tested E. coli, they showed (83) resistance to Tetracycline and 80 to Vancomycin. The resistance level of 13 isolated Salmonella was 69 to Nalidixic acid and 54 to Vancomycin. Multiple drug resistance was detected in high (98.4) for S. aureus, (56.7) for E. coli, and (53.9) for Salmonella. High proportion of multiple drug resistant in the dairy farm alerts concern for animal and public health as these drugs are used widely for treatment and prophylaxis in animals and humans.

A cross-sectional study design was employed on collected sera samples to investigate brucellosis in small ruminants from December 2018 to November 2019 with the objectives of estimating the seroprevalence and potential risk factors for the occurrence of brucellosis in small ruminants in selected districts of West Hararghe: Chiro, Hirna, and Mieso. A total of 2070 collected sera samples from small ruminants were tested using serological tests and screened by RBPT and confirmatory test (CFT). The overall seroprevalence of the present study was 0.24% in small ruminants (Chiro 0.2%, Hirna 0%, and Mieso 0.3%). The chi-square test (Stat 14.0) was used to determine the strength of potential risk factors associated with the occurrence of brucellosis by using univariable logistic regression. Mixed flock (OR = 2.11 (1.33–3.36 CI; )) , agropastoral (OR = 4.01 (2.35–6.84 CI; )) and pastoral (OR = 2.59 (1.37–4.90 CI; )) production system, and larger flock size (OR = 1.68 (1.08–2.60 CI; ) were factors significantly affecting the prevalence of small ruminant brucellosis. Univariable analysis was used, and independent predictors of small ruminant brucellosis were further analyzed using multilogistic regression. This disease was presented in the current study area; therefore, the careful separation of positive animals would help to prevent and control further distribution of the disease.

Background. Coronavirus and rotavirus are most commonly associated etiologies for calves diarrhoea, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by coronavirus and rotavirus in calves in Ethiopia are inadequately understood. A cross-sectional study was conducted with the aim of isolation and molecular characterization of coronavirus and rotavirus from calves in the central part of Oromia (Bishoftu, Sebata, Holeta, and Addis Ababa), Ethiopia, from November 2018 to May 2019. The four study areas were purposively selected and faecal samples were collected by simple random sampling for diagnosis of coronavirus and rotavirus infection by using the antigen detection enzyme-linked immunosorbent assay (Ag-ELISA) kit. In addition, this study was carried out to have insight in prevalence and associated risk factors of coronavirus and rotavirus infection in calves. Result. During the study, 83 diarrheic and 162 nondiarrheic faecal samples collected from calves less than 4 weeks of age were screened for coronavirus and rotavirus. Of the 83 diarrheic samples, 1 sample (1.2) was positive for coronavirus antigen and 6 samples (7.2) were found to be positive for rotavirus antigen by Ag-ELISA. All the nondiarrheic samples were negative for both coronavirus and rotavirus Ag. The overall prevalence of coronavirus and rotavirus infection in calves was estimated at 0.4 (1/245) and 2.45 (6/245), respectively. All samples (7) of ELISA test positive of both coronavirus and rotavirus were propagated in Madin-Darby bovine kidney (MDBK) cells. After 3 subsequent passages, progressive cytopathic effect (CPE), i.e., rounding, detachment, and the destruction of monolayer cell of five samples (1 sample of coronavirus and 4 samples of rotavirus) (71.4) were observed. At the molecular stage, reverse transcriptase polymerase chain reaction (RT-PCR) technique was used to determine the presence of coronavirus and rotavirus nucleic acid by using specific primers. The 5 samples that were coronavirus and rotavirus antigen positive by ELISA and develop CPE on cell culture were also positive on RT-PCR technique. The prevalence of infection peaked at 1st and 2nd weeks of age in male calves. Conclusion. Diarrheal disease caused by coronavirus and rotavirus has a great health problem in calves that interrupts production benefits with reduced weight gain and increased mortality and its potential for zoonotic spread. So, the present findings show coronavirus and rotavirus infection in calves in Ethiopia that needs to be addressed by practising early colostrum feeding in newborn calves, using vaccine, or improving livestock management.

Lumpy skin disease (LSD) is a highly contagious viral disease that causes significant economic losses in cattle populations globally. This study aimed to isolate and detect the LSD virus responsible for an outbreak in selected areas (Daaroo Labuu, Hawwii Guddina, and Gumbi Bordede district) of the West Hararghe Zone in Ethiopia between January 2020 and December 2021. Out of the 625 animals examined for the presence of LSD, only 73 animals showed clinical signs, and skin scrapes were collected from these animals for further analysis. Among those, 12 animals (1.9%) succumbed to the disease. Skin biopsy samples from 45 animals displaying clinical signs of LSD were inoculated in Vero cell lines because of limited equipment. After three blind passages, all samples developed cytopathic effects (CPEs). The presence of the LSD virus was confirmed using real‐time PCR. Conventional PCR detected LSDV in 47 (64.4%) of the skin scrap samples, while high‐resolution melt qPCR detected it in 49 (67.1%) samples. The study revealed a morbidity rate of 11.68%, a mortality rate of 1.92%, and a case fatality rate of 16.44% based on clinical data. The findings suggest that LSD causes significant economic losses, even in vaccinated animals prior to an outbreak. To effectively control and eradicate LSD, the government should develop new strategic policies. Community awareness campaigns are necessary to improve vector control measures and drainage systems. In addition, the present vaccination policy and strategy should be re‐evaluated for effectiveness. This study focused on a specific region and timeframe, limiting generalizability. Factors such as environmental conditions and management practices were not extensively explored. Similar studies should be conducted in different regions to assess the prevalence and genetic diversity of LSDV. The effectiveness of control measures and vaccination strategies should be investigated. The impact of environmental factors and management practices on LSD transmission and disease severity warrants further exploration. This study provides insights into the detection and isolation of the LSD virus during an outbreak in the West Hararghe Zone of Ethiopia. The results highlight the need for continued surveillance and monitoring of emerging infectious diseases in the region. Furthermore, the importance of using molecular methods for detecting and characterizing viral outbreaks in livestock populations is emphasized.