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Mrs. Chiara Muratore
Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, University of Milan

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0 Nitrogen
0 Proteomics
0 Plant Mineral Nutrition
0 Crops
0 Plant physiolgy

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Journal article
Published: 17 April 2021 in Plants
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In agricultural soils, nitrate (NO3 −) is the major nitrogen (N) nutrient for plants, but few studies have analyzed molecular and biochemical responses involved in its acquisition by grapevine roots. In viticulture, considering grafting, NO3 − acquisition is strictly dependent on rootstock. To improve the knowledge about N nutrition in grapevine, this study analyzed biochemical and proteomic changes induced by, NO3 − availability, in a hydroponic system, in the roots of M4, a recently selected grapevine rootstock. The evaluation of biochemical parameters, such as NO3 −, sugar and amino acid contents in roots, and the abundance of nitrate reductase, allowed us to define the time course of the metabolic adaptations to NO3 − supply. On the basis of these results, the proteomic analysis was conducted by comparing the root profiles in N-starved plants and after 30 h of NO3 − resupply. The analysis quantified 461 proteins, 26% of which differed in abundance between conditions. Overall, this approach highlighted, together with an increased N assimilatory metabolism, a concomitant rise in the oxidative pentose phosphate pathway and glycolysis, needed to fulfill the redox power and carbon skeleton demands, respectively. Moreover, a wide modulation of protein and amino acid metabolisms and changes of proteins involved in root development were observed. Finally, some results open new questions about the importance of redox-related post-translational modifications and of NO3 − availability in modulating the dialog between root and rhizosphere.

ACS Style

Bhakti Prinsi; Chiara Muratore; Luca Espen. Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability. Plants 2021, 10, 792 .

AMA Style

Bhakti Prinsi, Chiara Muratore, Luca Espen. Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability. Plants. 2021; 10 (4):792.

Chicago/Turabian Style

Bhakti Prinsi; Chiara Muratore; Luca Espen. 2021. "Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability." Plants 10, no. 4: 792.

Review
Published: 01 April 2021 in Plants
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Nitrogen nutrition in plants is a key determinant in crop productivity. The availability of nitrogen nutrients in the soil, both inorganic (nitrate and ammonium) and organic (urea and free amino acids), highly differs and influences plant physiology, growth, metabolism, and root morphology. Deciphering this multifaceted scenario is mandatory to improve the agricultural sustainability. In root cells, specific proteins located at the plasma membrane play key roles in the transport and sensing of nitrogen forms. This review outlines the current knowledge regarding the biochemical and physiological aspects behind the uptake of the individual nitrogen forms, their reciprocal interactions, the influences on root system architecture, and the relations with other proteins sustaining fundamental plasma membrane functionalities, such as aquaporins and H+-ATPase. This topic is explored starting from the information achieved in the model plant Arabidopsis and moving to crops in agricultural soils. Moreover, the main contributions provided by proteomics are described in order to highlight the goals and pitfalls of this approach and to get new hints for future studies.

ACS Style

Chiara Muratore; Luca Espen; Bhakti Prinsi. Nitrogen Uptake in Plants: The Plasma Membrane Root Transport Systems from a Physiological and Proteomic Perspective. Plants 2021, 10, 681 .

AMA Style

Chiara Muratore, Luca Espen, Bhakti Prinsi. Nitrogen Uptake in Plants: The Plasma Membrane Root Transport Systems from a Physiological and Proteomic Perspective. Plants. 2021; 10 (4):681.

Chicago/Turabian Style

Chiara Muratore; Luca Espen; Bhakti Prinsi. 2021. "Nitrogen Uptake in Plants: The Plasma Membrane Root Transport Systems from a Physiological and Proteomic Perspective." Plants 10, no. 4: 681.