Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments.

Maternal nutrition and lifestyle in pregnancy are important modifiable factors for both maternal and offspring’s health. Although the Mediterranean diet has beneficial effects on health, recent studies have shown low adherence in Europe. This study aimed to assess the Mediterranean diet adherence in 266 pregnant women from Dalmatia, Croatia and to investigate their lifestyle habits and regional differences. Adherence to the Mediterranean diet was assessed through two Mediterranean diet scores. Differences in maternal characteristics (diet, education, income, parity, smoking, pre-pregnancy body mass index (BMI), physical activity, contraception) with regards to location and dietary habits were analyzed using the non-parametric Mann–Whitney U test. The machine learning approach was used to reveal other potential non-linear relationships. The results showed that adherence to the Mediterranean diet was low to moderate among the pregnant women in this study, with no significant mainland–island differences. The highest adherence was observed among wealthier women with generally healthier lifestyle choices. The most significant mainland–island differences were observed for lifestyle and socioeconomic factors (income, education, physical activity). The machine learning approach confirmed the findings of the conventional statistical method. We can conclude that adverse socioeconomic and lifestyle conditions were more pronounced in the island population, which, together with the observed non-Mediterranean dietary pattern, calls for more effective intervention strategies.

Indoor air quality is a significant aspect of public health, yet it remains less studied than outdoor air pollution. Understudied indoor pollutants include volatile organic compounds (VOCs) and polycyclic aromatic hydrocarbons (PAHs). This review focuses on these two groups of compounds known for their health effects, including respiratory issues, neurological disorders, and carcinogenicity. We systematically compiled and analyzed data from studies reporting measured concentrations of VOCs and PAHs in European indoor environments—homes, schools, and offices—published in the past two decades. Concentration levels vary substantially across studies, influenced by regional differences, climate, building type, ventilation systems, and indoor activities. Identified sources include tobacco smoke, cooking, heating (e.g., biomass burning), and off‐gassing from construction and furnishing materials. Our analysis reveals clear geographic patterns: lower concentrations of VOCs and PAHs are consistently reported in Northern and Western European countries, likely due to stricter air quality regulations, cleaner outdoor air, greater use of electric heating, and more advanced ventilation systems. Conversely, higher concentrations are more commonly observed in Southern and Eastern Europe, where biomass heating and poorer ventilation remain more prevalent. Seasonal variation also has a significant role, with higher indoor levels typically measured during colder months due to increased heating and reduced air exchange. This highlights the need for improved indoor air quality management practices and regulatory standards to minimize the health risks associated with VOCs and PAHs. This review of 46 scientific publications is aimed at informing future studies and guiding future field measurements and risk assessments in epidemiological studies.