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We present a robust tool to analyze nonclassical properties of multimode twin-beam states in the mesoscopic photon-number domain. The measurements are performed by direct detection. The analysis exploits three different nonclassicality criteria for detected photons exhibiting complementary behavior in the explored intensity regime. Joint signal-idler photon-number distributions and quasidistributions of integrated intensities are determined and compared with the corresponding distributions of detected photons. Experimental conditions optimal for nonclassical properties of twin-beam states are identified.

It is shown that spatial correlation functions measured for correlated photon pairs at the single-photon level correspond to speckle patterns visible at high intensities. This correspondence is observed for the first time in one experimental setup by using different acquisition modes of an intensified CCD camera in low and high intensity regimes. The behavior of intensity auto- and cross-correlation functions in dependence on pump-beam parameters including power and transverse profile is investigated.

Cytosine methylation is a widespread epigenetic regulation mechanism. In healthy mature cells, methylation occurs at CpG dinucleotides within promoters, where it primarily silences gene expression by modifying the binding affinity of transcription factors to the promoters. Conversely, a recent study showed that in stem cells and cancer cell precursors, methylation also occurs at non-CpG pairs and involves introns and even gene bodies. The epigenetic role of such methylations and the molecular mechanisms by which they induce gene regulation remain elusive. The topology of both physiological and aberrant non-CpG methylation patterns still has to be detailed and could be revealed by using the differential stability of the duplexes formed between site-specific oligonucleotide probes and the corresponding methylated regions of genomic DNA. Here, we present a systematic study of the thermal stability of a DNA oligonucleotide sequence as a function of the number and position of non-CpG methylation sites. The melting temperatures were determined by monitoring the fluorescence of donor-acceptor dual-labelled oligonucleotides at various temperatures. An empirical model that estimates the methylation-induced variations in the standard values of hybridization entropy and enthalpy was developed.

We introduce an optical parametric amplifier, pumped by an amplified femtosecond Yb:KGW laser, which directly generates broadly tunable mid-infrared (MIR) pulses, covering the whole vibrational spectrum from 3 to 10 µm. The avoidance of the traditional difference-frequency generation stage to access the MIR range simplifies the setup while enabling high conversion efficiencies. The two-stage design employs in the second stage either periodically poled lithium niobate, optimized for the CH/OH stretching region (3-5 µm) or LiGaS₂, which allows extending the tunability to the fingerprint region (up to 10 µm). We anticipate applications of this versatile source to ultrafast vibrational spectroscopy and infrared microscopy.