Plants of the genus Allium developed a diversity of defense mechanisms against pathogenic fungi of the genus Fusarium, including transcriptional activation of pathogenesis-related (PR) genes. However, the information on the regulation of PR factors in garlic (Allium sativum L.) is limited. In the present study, we identified AsPR genes putatively encoding PR1, PR2, PR4, and PR5 proteins in A. sativum cv. Ershuizao, which may be involved in the defense against Fusarium infection. The promoters of the AsPR1–5 genes contained jasmonic acid-, salicylic acid-, gibberellin-, abscisic acid-, auxin-, ethylene-, and stress-responsive elements associated with the response to plant parasites. The expression of AsPR1c, d, g, k, AsPR2b, AsPR5a, c (in roots), and AsPR4a(c), b, and AsPR2c (in stems and cloves) significantly differed between garlic cultivars resistant and susceptible to Fusarium rot, suggesting that it could define the PR protein-mediated protection against Fusarium infection in garlic. Our results provide insights into the role of PR factors in A. sativum and may be useful for breeding programs to increase the resistance of Allium crops to Fusarium infections.

Chloroplasts of most plants are responsible for photosynthesis and contain a conserved set of about 110 genes that encode components of housekeeping gene expression machinery and photosynthesis-related functions. Heterotrophic plants obtaining nutrients from other organisms and their plastid genomes represent model systems in which to study the effects of relaxed selective pressure on photosynthetic function. The most evident is a reduction in the size and gene content of the plastome, which correlates with the loss of genes encoding photosynthetic machinery which become unnecessary. Transition to a non-photosynthetic lifestyle is expected also to relax the selective pressure on photosynthetic machinery in the nuclear genome, however, the corresponding changes are less known. Here we report the complete sequence of the plastid genome of Monotropa hypopitys, an achlorophyllous obligately mycoheterotrophic plant belonging to the family Ericaceae. The plastome of M. hypopitys is greatly reduced in size (35,336 bp) and lacks the typical quadripartite structure with two single-copy regions and an inverted repeat. Only 45 genes remained presumably intact– those encoding ribosomal proteins, ribosomal and transfer RNA and housekeeping genes infA, matK, accD and clpP. The clpP and accD genes probably remain functional, although their sequences are highly diverged. The sets of genes for ribosomal protein and transfer RNA are incomplete relative to chloroplasts of a photosynthetic plant. Comparison of the plastid genomes of two subspecies-level isolates of M. hypopitys revealed major structural rearrangements associated with repeat-driven recombination and the presence of isolate-specific tRNA genes. Analysis of the M. hypopitys transcriptome by RNA-Seq showed the absence of expression of nuclear-encoded components of photosystem I and II reaction center proteins, components of cytochrome b6f complex, ATP synthase, ribulose bisphosphate carboxylase components, as well as chlorophyll from protoporphyrin IX biosynthesis pathway. With the complete loss of genes related to photosynthesis, NADH dehydrogenase, plastid-encoded RNA polymerase and ATP synthase, the M. hypopitys plastid genome is among the most functionally reduced ones characteristic of obligate non-photosynthetic parasitic species. Analysis of the M. hypopitys transcriptome revealed coordinated evolution of the nuclear and plastome genomes and the loss of photosynthesis-related functions in both genomes.

Vegetables of the Allium genus are prone to infection by Fusarium fungi. Chitinases of the GH19 family are pathogenesis-related proteins inhibiting fungal growth through the hydrolysis of cell wall chitin; however, the information on garlic (Allium sativum L.) chitinases is limited. In the present study, we identified seven class I chitinase genes, AsCHI1–7, in the A. sativum cv. Ershuizao genome, which may have a conserved function in the garlic defense against Fusarium attack. The AsCHI1–7 promoters contained jasmonic acid-, salicylic acid-, gibberellins-, abscisic acid-, auxin-, ethylene-, and stress-responsive elements associated with defense against pathogens. The expression of AsCHI2, AsCHI3, and AsCHI7 genes was constitutive in Fusarium-resistant and -susceptible garlic cultivars and was mostly induced at the early stage of F. proliferatum infection. In roots, AsCHI2 and AsCHI3 mRNA levels were increased in the susceptible and decreased in the resistant cultivar, whereas in cloves, AsCHI7 and AsCHI5 expression was decreased in the susceptible but increased in the resistant plants, suggesting that these genes are involved in the garlic response to Fusarium proliferatum attack. Our results provide insights into the role of chitinases in garlic and may be useful for breeding programs to increase the resistance of Allium crops to Fusarium infections.

Eggplant (Solanum melongena L.) is an economically important vegetable crop whose purple-colored fruits are enriched with anthocyanidins. In this work in the eggplant genome, homologues of the main known structural (CHS1, CHS2, CHI, F3H, F3´5´H, DFR, ANS, and UFGT) and regulatory (TT8, GL3, bHLH137, bHLH143, MYB1, MYB2, and MYB75) anthocyanin biosynthesis genes, as well as anthocyanidin transporter gene (GSTF12), were identified. The expression of these genes was characterized in comparison with the content of the total anthocyanins and the color of the leaf, flower petals, and fruit peel. It was shown that the gene expression pattern corresponds to the color and the presence of anthocyanins in the tissue, and also indicates the presence of organ-specific characteristics of the regulation of transcription of genes encoding transcription factors of the MBW complex. The results of correlation analysis confirm the involvement of SmbHLH137, SmTT8, SmMYB2, and SmMYB75 genes in the regulation of the expression of structural genes in flower petals and SmGL3, SmTT8, and SmMYB1 in fruit peel.

Fruit biochemical analysis of four pepper (Capsicum annuum L.) cultivars, contrasting in immature and ripe fruit colour, was performed, and chlorophylls, carotenoids and anthocyanins were profiled in the peel and pulp during fruit ripening. It was found that pericarp parts (peel and pulp) differ in pigment composition and accumulation mode. In the same fruit tissues, the expression pattern of structural genes of the carotenoid (PSY1, PSY2, LCYb, and CCS) and anthocyanin (CHS, F3 '5 'H, DFR, ANS, and UFGT) pathways was evaluated. It was shown that carotenoid content positively correlates with the PSY1, LCYb and CCS expression level in the fruit pulp and with CCS expression level in the fruit peel. In pepper cultivars with purple-coloured immature fruits (Sirenevyj cub, Otello), a positive correlation was shown between the CHS, F3 '5 'H, DFR, ANS, and UFGT expression levels and the anthocyanin content in the peel. Thus, it was shown that in the pepper fruit, peel and pulp colours are regulated independently and determined by the main pigment ratio and the activity of carotenoid and anthocyanin biosynthesis genes.

Fusarium infection decreases the yield of garlic (Allium sativum L.); however, the knowledge about garlic response to fungal attack is limited. Chitosan induces plant defense response to stress conditions. Here, we analyzed the effects of chitosan with low (Ch1, 39 kDa) and medium (Ch2, 135 kDa) molecular weight on Fusarium infection in garlic. Ch1 and Ch2 at concentrations 0.125–0.400 mg/mL suppressed the growth of Fusarium proliferatum cultures in vitro. Pretreatment of garlic bulbs with Ch1 or Ch2 prevented disease symptoms after F. proliferatum inoculation, while exerting early inhibitory and late stimulatory effects on chitinase and β-1,3-glucanase activities. Ch1/Ch2 treatment of garlic already infected with F. proliferatum caused transcriptional upregulation of chitinases and β-1,3-glucanases at the early stage, which was maintained at the late stage in Ch2-treated samples, but not in Ch1-treated samples, where transcriptional inhibition was observed. The stimulatory effect of Ch2 pretreatment on the expression of chitinase and endo-β-1,3-glucanase genes was stronger than that of Ch1 pretreatment, suggesting that Ch2 could be more effective than Ch1 in pre-sowing treatment of garlic bulbs. Our results provide insights into the effects of chitosan on the garlic response to Fusarium, suggesting a novel strategy to protect garlic crop against fungal infection.