A new species of Infundibulicybe kotanensis from district Buner, Pakistan is described and illustrated. Phytogeographically this region is part of the floristically unique Sino-Japanese region. The cogent climate and diverse vegetation are favorable for the growth of different mushrooms. The new species is described based on morphological study and compared with closely related taxa in the genus. Phylogenetic placement using the nuclear ribosomal internal transcribed spacer (ITS) barcode region confirms the novelty of I. kotanensis.

Lentinus squarrosulus (Mont.) Singer: an edible macro-fungus is reported from the moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characteristics and nucleotide sequence analyses of the ITS region. Studies based on morpho-anatomical and molecular data are consistent with formerly reported specimens. Lentinus squarrosulus represents the first report for Pakistan.

Leucocoprinus atroferrugineus sp. nov. is described from Buner District, Pakistan, based on morphological features and molecular evidence. This species is characterized by the presence of dark, reddish-brown, areolate-fibrillose squamules on the pileus, which become dull reddish brown to reddish brown towards the margin; a hollow stipe with a pale pinkish apex; dark fibrils below the annulus, turn orange and eventually dark when scratched; and the presence of an annulus. The basidiospores are large, ellipsoid to amygdaliform. Detailed morphological characteristics and phylogenetic analysis using the ITS rDNA barcode region confirm the new species. A description with comprehensive discussion, illustrations, and phylogenetic analysis results of the new species are provided.

This article introduces Lactarius battagramensis as a novel species along with its ectomycorrhizal association from the temperate forests of Pakistan. Morpho-anatomical data coupled with sequence analyses and phylogeny inference of nrDNA ITS sequences are obtained from fruit bodies and ectomycorrhiza, leading to a better circumscription of new species. This new species is characterized by pale cream to vivid yellow and plano-concave to concave pileus, adnate lamellae that are concolorous to pileus, stipe that is tapering towards base, globose to broadly ellipsoid basidiospores forming partial to nearly complete reticulum, fusiform hymenial macrocystidia, absence of hymenial pseudocystidia, narrowly clavate paracystidia, and a hypoepithelium pileipellis. Phylogenetic estimation based on sequences from the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA) genes inferred that L. battagramensis formed a separate and distinct clade sister to L. mediterraneensis with a strong support value, which represents the uniqueness of this species from other species of L. subgen. Lactarius.

A new species, Entoloma fissuromarginatum, is described from the Battagram District, Khyber Pakhtunkhwa Province, Pakistan. The species is delineated based on the combination of morphological characteristics and molecular evidence, with nrITS sequence data supporting its distinct taxonomic status. Entoloma fissuromarginatum is characterized by a hemispherical to plano-concave and light brownish-gray pileus with split margins, adnate and light brownish-gray lamellae, reddish brown and scaly stipe, heterodiametric basidiospores (average 6.40 × 5.61 μm) having 5–6 angles, absence of pleurocystidia, lageniform cheilocystidia, narrowly clavate to subcapitate caulocystidia, and a cutis pileipellis and stipitipellis. A description, phylogenetic analysis results, an illustration, and a comparison with closely related taxa of the new species are provided.