Extracellular enzymes play an important role in the growth and development of edible fungi. Extracellular enzyme activities have also become an important object of measurement. In this study, Agaricus brunnescens Peck, Coprinus comatus, and Pleurotus ostreatus were compared in terms of their enzyme production in liquid-and solid-state fermentation. Differences in the ability of various types of edible fungi to utilize biomass raw materials were analyzed by monitoring the fiber degradation rate during crop straw degradation, and changes in their cellulolytic enzyme systems during growth and metabolism were discussed. This study provided insights into the changes in the lignocellulose degradation ability of edible fungi during their growth and facilitated the discovery of new approaches to accelerate their growth in culture.
17 March 2023 in International Journal of Biological Macromolecules
To evaluate pharmacological activities of a novel fungus Hygrophoropsis sp., the influence of aeration rate on the production of mycelial biomass, exopolysaccharides (EPS), and intrapolysaccharides (IPS) in the fungus Hygrophoropsis sp. was investigated. And the water extracts of cultured Hygrophoropsis sp. mycelia and the fermentation broth were analyzed for their antioxidation activity by using four different assay methods such as hydroxyl radical scavenging, superoxide radical scavenging, hydrogen peroxide scavenging, and reducing power. The Hygrophoropsis sp. was cultivated under various aeration rates in a 7l bioreactor. The highest mycelial biomass (3.98mg/mL) and IPS production (19.63mg/g) were obtained at aeration rate 4.5 v.v.m. The results showed that Hygrophoropsis sp., in general, possesses a strong antioxidation activity in all assays tested. The deproteinized extracts had stronger antioxidation activity as compared to the un-deproteinized extracts by using superoxide radical scavenging, hydrogen peroxide scavenging, and reducing power. Besides, the un-deproteinized extracts had stronger antioxidation activity as compared to the deproteinized extracts by using hydroxyl radical scavenging. Thus, the polysaccharide extractions from the Hygrophoropsis sp. studied have antioxidant activities in vitro, which may be a good source of natural antioxidants or further investigation as potential natural antioxidants.
In this paper, different proportion of wormcast was added to the Rehmannia glutinosa continuous cropping soils including first and second stubble to adjust the phenolic acids content and microbial biomass and improve the soil environment after continuous cropping. Results showed that vanillic acid, vanillin and ferulic acid in the soils cropped Rehmannia glutinosa for one year were all somewhat decreased after a growth cycle, half a year. The ratio of fungi to bacteria was firstly increased then decreased in the soils cropped Rehmannia glutinosa for one year, while an opposite result in the second stubble soil after addition of wormcast. Taken together, the effect, 10 percent addition of wormcast to the soil cropped Rehmannia glutinosa for two years, was the best.