In vitro techniques, with the purpose of conserving the genetic resources of plants, are fundamental to the feasibility of establishing germplasm banks and enabling the commercial production of micropropagated plants. The aim of this study was to evaluate the use of different spectra of light in in vitro germplasm conservation by slow grown storage of Heliconia Champneiana cv. Splash. Explants of heliconia in vitro were submitted to the following light treatments: CW (control white), B100 (100 % blue), R100 (100 % red) and R70B30 (70 % red/30 % blue), all with PFD = 25 μmol m2 s–1, maintained in vitro for two time periods, namely P1, 6 weeks and P2, twelve weeks, and were statistically evaluated after each interval with respect to: height, fresh weight, number and length of roots, number of leaves, and pseudostem diameter, in addition to the rate of acclimatization in percentage terms. Treatment B100 presented the lowest level of development in the two periods, and 100 % survival in acclimatization.

The lisianthus culture is demanded to produce cut flowers and pot vases, grown in a greenhouse using advanced techniques. The present study aimed to evaluate the pigments of different lisianthus cultivars in different photoselective shade nets. The cultivars Flare Deep Rose (pink), Echo Blue BL (purple) and Allemande White BI (white) were cultivated under light-diffusing polyethylene film cover and blue and red color photoselective nets with a shading capacity of 30%, in addition to the control treatment, without the use of the net. The results showed higher values of chlorophyll b and total in the red photoselective net, independent of the cultivar. Leaf colorimetry showed the most intense shade of green was from the cultivar Echo Blue BL. On the other hand, the control showed more intensity, with no significant difference in the leaf green intensity on the blue and red shade nets. In the petals, the cultivar Echo Blue BL showed a more intense blue shade in the red net, however low grade of anthocyanin than in the blue one. In the anthocyanin pigment, this cultivar had lower levels in the treatment of the red net. The results indicate that the use of photoselective shade nets in the culture of lisianthus changes the content of the pigments studied, in varying intensity, depending on the cultivar.

O objetivo do presente trabalho foi avaliar, em condições de campo, características horticulturais de mudas propagadas in vitro de Sacha inchi (Plukenetia volubilis L), quanto à época de florescimento e colheita, características morfológicas relacionadas à coloração e forma de folhas, caule e frutos, bem como a produtividade do material propagado in vitro. As mudas de cultura de tecidos foram propagadas por seis semanas em meio de cultivo com sais e vitaminas de MS, acrescido de 30g L-1 de sacarose e 1,0mg L-1 de BAP, a partir do ponteiro de plântula germinada in vitro. Foram selecionadas, ao acaso, quatro plantas propagadas in vitro, quatro plantas por via seminal e mais vinte e duas plantas, também por via seminal, compondo a bordadura, totalizando trinta plantas. As análises foram semanais até completar quatorze meses do plantio, que ocorreu antes da primeira poda. Os resultados obtidos indicam a não ocorrência de variabilidade genética nas plantas produzidas in vitro, bem como precocidade na produção e maior produtividade, quando comparado com o material convencional obtido via seminal.

O objetivo deste trabalho foi avaliar o efeito de diferentes comprimentos de onda no desenvolvimento in vitro de mudas de cana-de-açúcar. Explantes foram submetidos a quatro tratamentos de diodos emissores de luz (LED): 100% azul; 70% azul + 30% vermelha; 30% azul + 70% vermelha; 100% vermelha, além do controle com lâmpada fluorescente branca. As plântulas foram avaliadas quanto a: número de brotações; altura; massa de matéria fresca e seca; e conteúdo de carotenoides e das clorofilas a e b. Observou-se desmanche dos tilacoides nos cloroplastos, proporcional ao aumento na incidência de luz vermelha. O porte das mudas diminui com o aumento na incidência de luz vermelha.