In recent years, the alternating occurrence of high temperature and waterlogging disasters in South China has seriously reduced the yield of single cropping rice. Studying the changes in anatomical structure of the rice root system could provide theoretical basis for understanding the mechanisms of high temperature and waterlogging stress. To examine interactions between temperature and waterlogging stress, an experiment was set up in a growth chamber consisting of two temperatures (moderate, 30–34 °C and high, 35–38 °C) with three depths of flooding (0–5, 10 and 15 cm). Treatments commenced at the booting stage and lasted five days, after which all treatments were returned to a 0–5 cm flooding depth and the same temperature regime. Observations were made immediately after cessation of treatments, then after 5, 10 and 20 d to test the effect of treatments on subsequent root anatomical development. The low-stress control (0–5 cm, medium temperature) showed no change with time in aerenchyma area, thickness of the outer root, stele diameter, and the number nor diameter of xylem vessels. Root diameter and stele diameter under the high-stress control (0–5 cm, high temperature) were decreased by 29.09% and 15.28%, respectively, at the booting stage, whereas the high stress control (15 cm, high temperature) affected only the vessel diameter, reducing it by 14.11% compared with that in the low-stress control (0–5 cm, medium temperature). Compared to the high-stress control (0–5 cm, high temperature), the interaction of high temperature and waterlogging stress alleviated the inhibiting effect of the changes in the root system, especially after the end of the stress. We thought that waterlogging could reduce the damage of high temperature on rice root growth. Low water depth waterlogging has little effect on rice root system and aerenchyma area root diameter at 0, 5, 10 and 20 d after the stress ended, and the thickness of the outer root, stele diameter and the number and diameter of vessels at 0 d under M15 (15 cm, medium temperature) had no significant difference compared with M5 (0–5 cm, medium temperature). However, the increase in rice root diameter, stele diameter, thickness of the outer root cortex depth and vessel diameter were inhibited under high temperature stress at the booting stage. Root diameter and stele diameter under H5 (0–5 cm, high temperature) were decreased by 29.09% and 15.28%, respectively, at the booting stage, whereas H15 (15 cm, high temperature) affected only the vessel diameter, reducing it by 14.11% compared with that in the M5. Compared to H5, the interaction of high temperature and waterlogging stress alleviated the inhibiting effect of the changes in the root system, especially after the end of the stress. We thought that waterlogging could lighten the damage of high temperature on rice root growth.

The global demand for cotton makes sustainable cotton production an important issue that can be improved by a better understanding of the influence of soil microbes on cotton growth. We collected cotton field soils at the seedling and flowering/boll-setting (FBS) stages in order to obtain soil properties and cotton growth indices. Bacterial and fungal community compositions were assessed by high-throughput sequencing of 16S rRNA and internal transcribed spacer genes, respectively, after which the differences in microbial functions and their influencing factors at different growth stages were analyzed. Both the diversity and composition of soil bacterial and fungal communities were found to be significantly different between the seedling and FBS stages. Microbes in the seedling stage had significantly higher richness and biomass than those in the FBS stage. Compared with the seedling stage, the stability of the soil bacterial communities was decreased. The cotton growth indices at both the seedling and FBS stages were associated with compositional shifts in the bacterial community and but not the fungal community. The abundance of specific soil microbial taxa (e.g., Pseudarthrobacter, Thiobacillus, Cephalotrichum, Chaetomium, and Fusarium) were correlated with cotton growth indices at the seedling stage, being mainly regulated by soil salinity and nitrate content. Our results highlight the importance of soil microbial communities in mediating cotton growth and will be useful in providing better strategies for the improvement of cotton agriculture.

This study aimed to investigate the legacy effects of prolonged brackish irrigation on rhizobacterial communities and agricultural productivity in wheat. Here, we conducted pot experiments to investigate the mechanisms through which different irrigation regimes (irrigation using brackish groundwater and normal water) regulate wheat production. We applied four irrigation treatments across different stages of wheat growth (early stages, seedling-to-jointing, and late stages, jointing-to-maturity). This included irrigation exclusively using normal water during both stages (RR), using normal water followed by brackish groundwater (RW), exclusively using brackish groundwater (WW), and using brackish groundwater followed by normal water (WR). Under the premise of retaining 10 seedlings per pot, the average number of effective spikes per 10 plants in the RR, RW, and WR treatments was approximately 1.3, 1.1, and 1.1 times that of WW (19 ± 1), respectively. The spike weight per 10 plants in the RR, RW, and WR treatments was approximately 1.8, 1.5, and 1.3 times that of WW (12.75 ± 1.74 g), respectively. Compared with brackish groundwater irrigation, the use of normal water during the early stages significantly reduced the relative abundance of Pseudomonadota and increased that of Chloroflexota (p < 0.05). The number of effective spikes was positively correlated with the relative abundances of Actinobacteriota, Acidobacteriota, Chloroflexota, and Bacteroidota, but negatively correlated with the abundance of Pseudomonadota (p < 0.05). Irrigation regimes altered the rhizobacterial community structure. However, the legacy effect of long-term irrigation using brackish groundwater resulted in the dominance of stochastic processes in bacterial community assembly and stability of the Shannon diversity across all treatments. The complexity of the rhizobacterial co-occurrence network was lower in the RW treatments than that in the WW treatments (p < 0.05). Structural equation modeling revealed that irrigation using normal water during early stages boosted the number of effective spikes in wheat. This enhancement was achieved by increasing rhizobacterial diversity, reducing rhizosphere sodium, and simplifying the microbial network. This study challenges the “legacy effect” of brackish water irrigation by demonstrating that optimal irrigation timing is key to enhancing crop yield.