This page has only limited features, please log in for full access.
Sami Souissi has a Ph.D (1998) in Biological Oceanography and Marine Environment from the University Pierre et Marie Curie (Paris VI), France. In 2006, he obtained the Habilitation degree in Natural Sciences at the University of Lille 1, France. Since 2008, he has been a full Professor at that university.
Copepods represent an interesting alternative or a complement live food to brine shrimps and rotifers commonly used in aquaculture. They constitute the natural prey of many fish species and therefore do not require a potential nutritional enrichment. But an optimization of the microalgal diets used to feed copepods is essential to improve their mass culture. This study examined the effects of seven microalgal diets, namely single-species diets of Rhodomonas salina (R), Tisochrysis lutea (T), and Pavlova lutheri (=Diacronema lutheri) (P), two-species diets (R + T, T + P, and R + P), and a three-species diet (R + T + P), on the fatty acid and monosaccharide composition of the cyclopoid copepod Paracyclopina nana as well as its reproductive investment. Experiments were run during 15 days in 10-L beakers; starting with nauplii collected from a large 300-L batch culture. Copepods fatty acid contents were studied, particularly the relative amounts of docosahexaenoic acid (DHA) and eicosa-pentaenoic acid (EPA). The R + T, R, and T diets induced the highest total fatty acid amount in copepods. R + T and R also generated the lowest DHA/EPA ratios in copepods due to high EPA contents. The highest value of total monosaccharides was found in copepods fed with R + T + P. Diets R + T and R induced the greatest prosome volumes and clutch volumes in ovigerous females. Both prosome volume and clutch volume in P. nana ovigerous females were correlated to the individual EPA amount. The results demonstrated that all diets including R. salina enhanced the productivity of P. nana in mass culture, particularly when combined with T. lutea. R. salina, and T. lutea induced complementary fatty acid and monosaccharide profiles, confirming that R + T represents the best microalgae combination for productive culture of P. nana. Conversely, P. lutheri did not enhance the nutritional profile nor the fecundity of P. nana in the culture. This study is the first to demonstrate that R. salina is a suitable microalga for productive mass culture of P. nana for use as live food in aquaculture.
Paul Dayras; Capucine Bialais; Irina Sadovskaya; Min-Chul Lee; Jae-Seong Lee; Sami Souissi. Microalgal Diet Influences the Nutritive Quality and Reproductive Investment of the Cyclopoid Copepod Paracyclopina nana. Frontiers in Marine Science 2021, 8, 1 .
AMA StylePaul Dayras, Capucine Bialais, Irina Sadovskaya, Min-Chul Lee, Jae-Seong Lee, Sami Souissi. Microalgal Diet Influences the Nutritive Quality and Reproductive Investment of the Cyclopoid Copepod Paracyclopina nana. Frontiers in Marine Science. 2021; 8 ():1.
Chicago/Turabian StylePaul Dayras; Capucine Bialais; Irina Sadovskaya; Min-Chul Lee; Jae-Seong Lee; Sami Souissi. 2021. "Microalgal Diet Influences the Nutritive Quality and Reproductive Investment of the Cyclopoid Copepod Paracyclopina nana." Frontiers in Marine Science 8, no. : 1.
The development of sentinel species in aquatic ecosystems is mostly based on benthic organisms; however, organisms living in water column such as zooplankton have received less attention, except for some cladocerans. In this paper, a new ecological indicator based on simple measurements of the size and fecundity of egg-bearing calanoid copepods is developed. The well-studied estuarine copepod Eurytemora affinis is used to illustrate this new framework. A large database obtained from laboratory experiments developed under different conditions is used to define a reference regression line between clutch size (CS) and prosome length (PL). The same database allowed one to confirm that the coefficient of variation (CV) of CS is an adequate estimator of the accumulated stress at population level. The CV of PL shows very little variability in all experimental and field conditions. The values of CS and PL obtained from the Seine, Loire, Gironde, Scheldt and Elbe estuaries in Europe are compared to the reference regression line. A quality index (QI) is calculated as a percentage of difference between the observed and the predicted CS. The QI classified 19 samples collected in the Seine estuary between 2004 and 2010 into four classes according to the physiological condition of the copepod female. A single sampling from June 2004 (5.26%) showed a very good condition, whereas 57.9% of the sampling dates confirmed good conditions. On the other hand, four sampling dates were associated to very bad conditions and three sampling dates indicated bad conditions. Seven additional samples obtained from other European estuaries between 2006 and 2009 were also used. Females showing poor conditions were observed in the early spring of 2005 and 2008 as well as during the month of November. These years were characterized by very strong climatic anomalies with a very cold late winter in 2005 and a warm winter in 2008. Therefore, it seems that the QI perfectly reflected the strong stress caused by the sudden change in hydro-climatic conditions that have certainly affected the physiology of copepod females and probably the availability of food. The new indicator is very simple to calculate and can be generalised to several aquatic ecosystems (fresh water and brackish water) by targeting the dominating egg-bearing calanoid copepods. As in the case of E. affinis, the development of sentinel species based on copepods or cladocerans can enrich ecological and ecotoxicological studies given their capacity to integrate the variability of their habitats’ quality at the individual and population levels.
Sami Souissi; Anissa Souissi. Promotion of the Development of Sentinel Species in the Water Column: Example Using Body Size and Fecundity of the Egg-Bearing Calanoid Copepod Eurytemora affinis. Water 2021, 13, 1442 .
AMA StyleSami Souissi, Anissa Souissi. Promotion of the Development of Sentinel Species in the Water Column: Example Using Body Size and Fecundity of the Egg-Bearing Calanoid Copepod Eurytemora affinis. Water. 2021; 13 (11):1442.
Chicago/Turabian StyleSami Souissi; Anissa Souissi. 2021. "Promotion of the Development of Sentinel Species in the Water Column: Example Using Body Size and Fecundity of the Egg-Bearing Calanoid Copepod Eurytemora affinis." Water 13, no. 11: 1442.
Planktonic copepods are tiny crustaceans, with a typical size of the order of mm, living in suspension in marine or freshwaters during their entire life cycle. They have swimming and jumping abilities and are known to be well adapted to their turbulent environment. Turbulence is known to increase their contact rate and feeding flux. However too intense turbulence is believed to have a negative effect so that a qualitative bell-shape is classically invoked to represent the contact rate of copepods versus turbulence intensity. In this framework, the objective of this work is to quantify the influence of ambient turbulence on copepod’s behavior, using trajectory analysis.
In this work, the motions of copepods were filmed using an infrared high-speed camera (1000 fps) in a turbulent environment, in the dark to avoid phototropism. The custom-made experimental set-up has been built-up in order to obtain in a central zone an isotropic and homogeneous turbulence representative of the natural environment. The flow was characterized with different tracer sizes at different turbulence intensities.
Copepods are filmed and the trajectories are extracted using signal processing routines. The instantaneous velocity, tangential and centripetal accelerations, and the local curvature are extracted for each trajectory. Their pdfs are computed, as well as different statistical moments: these indicators are studied at varying the turbulence intensity level (Reynolds number). Particles of different sizes (100 and 600 microns of mean diameters) and dead copepods are compared to living copepods statistics. This strategy allows to precisely characterize the copepods behavioral activity in relation with ambient turbulence. Ecological interpretations are drawn from the experimental results.
Clotilde Le Quiniou; François Schmitt; Yongxiang Huang; Enrico Calzavarini; Sami Souissi. Copepods in turbulence: laboratory velocity and acceleration studies using high speed cameras. 2021, 1 .
AMA StyleClotilde Le Quiniou, François Schmitt, Yongxiang Huang, Enrico Calzavarini, Sami Souissi. Copepods in turbulence: laboratory velocity and acceleration studies using high speed cameras. . 2021; ():1.
Chicago/Turabian StyleClotilde Le Quiniou; François Schmitt; Yongxiang Huang; Enrico Calzavarini; Sami Souissi. 2021. "Copepods in turbulence: laboratory velocity and acceleration studies using high speed cameras." , no. : 1.
Chun‐Wei Chang; Hao Ye; Takeshi Miki; Ethan R. Deyle; Sami Souissi; Orlane Anneville; Rita Adrian; Yin‐Ru Chiang; Satoshi Ichise; Michio Kumagai; Shin‐Ichiro S. Matsuzaki; Fuh‐Kwo Shiah; Jiunn‐Tzong Wu; Chih‐Hao Hsieh; George Sugihara. Long‐term warming destabilizes aquatic ecosystems through weakening biodiversity‐mediated causal networks. Global Change Biology 2020, 26, 6413 -6423.
AMA StyleChun‐Wei Chang, Hao Ye, Takeshi Miki, Ethan R. Deyle, Sami Souissi, Orlane Anneville, Rita Adrian, Yin‐Ru Chiang, Satoshi Ichise, Michio Kumagai, Shin‐Ichiro S. Matsuzaki, Fuh‐Kwo Shiah, Jiunn‐Tzong Wu, Chih‐Hao Hsieh, George Sugihara. Long‐term warming destabilizes aquatic ecosystems through weakening biodiversity‐mediated causal networks. Global Change Biology. 2020; 26 (11):6413-6423.
Chicago/Turabian StyleChun‐Wei Chang; Hao Ye; Takeshi Miki; Ethan R. Deyle; Sami Souissi; Orlane Anneville; Rita Adrian; Yin‐Ru Chiang; Satoshi Ichise; Michio Kumagai; Shin‐Ichiro S. Matsuzaki; Fuh‐Kwo Shiah; Jiunn‐Tzong Wu; Chih‐Hao Hsieh; George Sugihara. 2020. "Long‐term warming destabilizes aquatic ecosystems through weakening biodiversity‐mediated causal networks." Global Change Biology 26, no. 11: 6413-6423.
Multiple stressors like metal toxicity, organic compounds and sediment pollution from the Seine estuary are raising concern and novel toxicological approaches are needed to better assess and monitor the risk. In the present study, the copepod Eurytemora affinis from the Seine, was exposed to two different sources of contaminants, which were resuspended polluted sediments and a mixture of trace metals (dissolved phase). The exposure continued for four generations (F0, F1, F2, F3) where F0 is a generation for acclimation to the exposure condition and F3 is a generation for decontamination followed without any exposure, to detect possible maternal carryover effects of pollutants (F0 – F2) and the role of recovery (in F3). Higher accumulation of metals resulted in higher mortalities at both exposure conditions, with particularly F1 being the most sensitive generation showing highest bioaccumulation of metals, highest mortality, and smallest population size. Copper accumulation was highest of all metals in mixture from both the resuspended sediment and the combined trace metal treatment. A significantly lower naupliar production was seen in copepods exposed to resuspended sediment compared to trace metal exposed copepods. However, the decontamination phase (F3) indicated that E. affinis pre-exposed to resuspended sediment had a higher ability to recover the total population size, increase naupliar production, and depurate accumulated Cu. The population exposed to a trace metal mixture showed lower recovery and lower ability to discharge accumulated toxic metals indicating its greater effect on our experimental model when compared to resuspended sediment.
Shagnika Das; Baghdad Ouddane; Jiang-Shiou Hwang; Sami Souissi. Intergenerational effects of resuspended sediment and trace metal mixtures on life cycle traits of a pelagic copepod. Environmental Pollution 2020, 267, 115460 .
AMA StyleShagnika Das, Baghdad Ouddane, Jiang-Shiou Hwang, Sami Souissi. Intergenerational effects of resuspended sediment and trace metal mixtures on life cycle traits of a pelagic copepod. Environmental Pollution. 2020; 267 ():115460.
Chicago/Turabian StyleShagnika Das; Baghdad Ouddane; Jiang-Shiou Hwang; Sami Souissi. 2020. "Intergenerational effects of resuspended sediment and trace metal mixtures on life cycle traits of a pelagic copepod." Environmental Pollution 267, no. : 115460.
In this study, the whole transcriptome and sex-specific differential gene expression of the copepod Pseudodiaptomus annandalei exposed to cadmium (Cd) were investigated. P. annandalei were exposed to 40 μg/L Cd from the naupliar stage to male and female adults. High-throughput transcriptome sequencing (RNA-seq) was performed with copepod samples using an Illumina Hiseq™ 2000 platform. TransDecoder analysis found 32,625 putative open reading frame contigs. At p-values of <0.001, a total of 4756 differentially expressed genes (DEGs) (2216 up-regulated and 2540 down-regulated genes) were found in male copepods. Whereas a total of 2879 DEGs (2007 up-regulated and 872 down-regulated genes) were found in female copepods. A few selected cellular stress response genes, involved in xenobiotic metabolism, energy metabolism, growth, and development as a result of Cd exposure in the copepods were discussed. The study showed that most of these processes were changed in a sex-specific manner, accounting for the different sensitivities of male and female copepods. Results suggest and reinforce that sex is an important factor to be considered in ecotoxicogenomics.
Esther Kadiene; Baghdad Ouddane; Hong-Yi Gong; Min-Sub Kim; Jae-Seong Lee; Yen-Ju Pan; Jiang-Shiou Hwang; Sami Souissi. Differential gene expression profile of male and female copepods in response to cadmium exposure. Ecotoxicology and Environmental Safety 2020, 204, 111048 .
AMA StyleEsther Kadiene, Baghdad Ouddane, Hong-Yi Gong, Min-Sub Kim, Jae-Seong Lee, Yen-Ju Pan, Jiang-Shiou Hwang, Sami Souissi. Differential gene expression profile of male and female copepods in response to cadmium exposure. Ecotoxicology and Environmental Safety. 2020; 204 ():111048.
Chicago/Turabian StyleEsther Kadiene; Baghdad Ouddane; Hong-Yi Gong; Min-Sub Kim; Jae-Seong Lee; Yen-Ju Pan; Jiang-Shiou Hwang; Sami Souissi. 2020. "Differential gene expression profile of male and female copepods in response to cadmium exposure." Ecotoxicology and Environmental Safety 204, no. : 111048.
Microplastics (MPs), plastics with particles smaller than 5 mm, have been found almost in every corner of the world, especially in the ocean. Due to the small size, MPs can be ingested by animals and enter the marine trophic chain. MPs can affect animal health by physically causing damage to the digestive tract, leaking plastic chemical components, and carrying environmental pollutants and pathogens into animals. In this study, impacts of MPs ingestion on gut microbiota were investigated. Filter feeding mussels were exposed to “virgin” and “weathered” MPs at relatively realistic concentration 0.2 mg L−1 (“low”) and exaggerated concentration 20 mg L−1 (“high”) for 6 weeks. Influence in mussel gut microbiota was investigated with 16S rRNA gene high-throughput sequencing. As compared with non-exposed mussels, alteration of gut microbiota was observed after mussels were exposed to MPs for 1 week, 3 weeks, 6 weeks, and even after 8-day post-exposure depuration. Potential human pathogens were found among operational taxonomic units (OTUs) with increased abundance induced by MP-exposure. Faecal pellets containing microorganisms from altered gut microbiota and MPs might further influence microbiota of surrounding environment. Our results have demonstrated impacts of MP-exposure on mussel gut microbiota and suggested possible consequent effects on food quality, food safety, and the well-being of marine food web in the ecosystem for future studies.
Luen-Luen Li; Rachid Amara; Sami Souissi; Alexandre Dehaut; Guillaume Duflos; Sébastien Monchy. Impacts of microplastics exposure on mussel (Mytilus edulis) gut microbiota. Science of The Total Environment 2020, 745, 141018 .
AMA StyleLuen-Luen Li, Rachid Amara, Sami Souissi, Alexandre Dehaut, Guillaume Duflos, Sébastien Monchy. Impacts of microplastics exposure on mussel (Mytilus edulis) gut microbiota. Science of The Total Environment. 2020; 745 ():141018.
Chicago/Turabian StyleLuen-Luen Li; Rachid Amara; Sami Souissi; Alexandre Dehaut; Guillaume Duflos; Sébastien Monchy. 2020. "Impacts of microplastics exposure on mussel (Mytilus edulis) gut microbiota." Science of The Total Environment 745, no. : 141018.
Yen‐Ju Pan; Emilien Déposé; Anissa Souissi; Stéphane Hénard; Mike Schaadt; Ed Mastro; Sami Souissi. Assessments of first feeding protocols on the larviculture of California grunion Leuresthes tenuis (Osteichthyes: Atherinopsidae). Aquaculture Research 2020, 51, 3054 -3058.
AMA StyleYen‐Ju Pan, Emilien Déposé, Anissa Souissi, Stéphane Hénard, Mike Schaadt, Ed Mastro, Sami Souissi. Assessments of first feeding protocols on the larviculture of California grunion Leuresthes tenuis (Osteichthyes: Atherinopsidae). Aquaculture Research. 2020; 51 (7):3054-3058.
Chicago/Turabian StyleYen‐Ju Pan; Emilien Déposé; Anissa Souissi; Stéphane Hénard; Mike Schaadt; Ed Mastro; Sami Souissi. 2020. "Assessments of first feeding protocols on the larviculture of California grunion Leuresthes tenuis (Osteichthyes: Atherinopsidae)." Aquaculture Research 51, no. 7: 3054-3058.
This study investigated the effects of seven microalgal diets, including single‐species diets of Rhodomonas salina (R), Tisochrysis lutea (T), and Pavlova lutheri (P), two‐species diets (R + T, T + P, and R + P), and a three‐species diet (R + T + P), on the cyclopoid copepod Paracyclopina nana growth, stage composition, and ovigerous female size and fecundity. Experiments were conducted in 10‐L beakers starting with nauplii from a 300‐L culture population. After 15 days of feeding, the R + T diet induced the greatest total population growth, particularly for copepodites and all female stages (preadult females, females, and ovigerous females). The R diet followed by R + T also generated the greatest individual size and clutch sizes in ovigerous females. Overall results show that the diets leading to the highest total population growth and clutch sizes in P. nana included R. salina. Conversely, P. lutheri was not a very effective microalga to enhance P. nana productivity in culture. Current results are the first to show that R. salina is a suitable microalga for production of P. nana for use as live food for marine fish larval aquaculture.
Paul Dayras; Capucine Bialais; Jae‐Seong Lee; Sami Souissi. Effects of microalgal diet on the population growth and fecundity of the cyclopoid copepod Paracyclopina nana. Journal of the World Aquaculture Society 2020, 51, 1386 -1401.
AMA StylePaul Dayras, Capucine Bialais, Jae‐Seong Lee, Sami Souissi. Effects of microalgal diet on the population growth and fecundity of the cyclopoid copepod Paracyclopina nana. Journal of the World Aquaculture Society. 2020; 51 (6):1386-1401.
Chicago/Turabian StylePaul Dayras; Capucine Bialais; Jae‐Seong Lee; Sami Souissi. 2020. "Effects of microalgal diet on the population growth and fecundity of the cyclopoid copepod Paracyclopina nana." Journal of the World Aquaculture Society 51, no. 6: 1386-1401.
We examined effects of the three metals cadmium (Cd), copper (Cu), and nickel (Ni) on two subpopulations of the cyclopoid copepod Paracyclopina nana. We sought to investigate the effects of metal exposure on population growth and structure of P. nana and to understand the parameters affecting the metal bioaccumulation in copepods. A first experiment tested the hypothesis of competition between these metals in a mixture using a P. nana mass culture in 10 L beakers with the sublethal concentrations (1/3 of LC50) as determined for E. affinis. A second experiment pursued the same with a P. nana population which was adapted to a higher Cu concentration for several generations (226.9 ± 15.9 μg g−1 dw Cu in copepods) and using the proper sublethal concentrations for P. nana. After 96 h of exposure, results from the first experiment showed a decreasing population growth and instead of an increasing metal accumulation in copepods. Cd also appeared to be more accumulated when it was alone, confirming the hypothesis of metal competition in mixture. Results from the second experiment revealed less marked effects. When metal concentrations increased in the treatment it decreased in copepods, indicating depuration activity in the population already adapted to metal exposure. This paper is the first one investigating the parameters affecting the bioaccumulation capacity of P. nana in response to metals. It offers a better understanding of copepod responses to metal contamination in a complex aquatic environment.
Paul Dayras; Capucine Bialais; Baghdad Ouddane; Jae-Seong Lee; Sami Souissi. Effects of different routes of exposure to metals on bioaccumulation and population growth of the cyclopoid copepod Paracyclopina nana. Chemosphere 2020, 248, 125926 .
AMA StylePaul Dayras, Capucine Bialais, Baghdad Ouddane, Jae-Seong Lee, Sami Souissi. Effects of different routes of exposure to metals on bioaccumulation and population growth of the cyclopoid copepod Paracyclopina nana. Chemosphere. 2020; 248 ():125926.
Chicago/Turabian StylePaul Dayras; Capucine Bialais; Baghdad Ouddane; Jae-Seong Lee; Sami Souissi. 2020. "Effects of different routes of exposure to metals on bioaccumulation and population growth of the cyclopoid copepod Paracyclopina nana." Chemosphere 248, no. : 125926.
Orlane Anneville; Chun‐Wei Chang; Gaël Dur; Sami Souissi; Frédéric Rimet; Chih‐Hao Hsieh. The paradox of re‐oligotrophication: the role of bottom–up versus top–down controls on the phytoplankton community. Oikos 2019, 128, 1666 -1677.
AMA StyleOrlane Anneville, Chun‐Wei Chang, Gaël Dur, Sami Souissi, Frédéric Rimet, Chih‐Hao Hsieh. The paradox of re‐oligotrophication: the role of bottom–up versus top–down controls on the phytoplankton community. Oikos. 2019; 128 (11):1666-1677.
Chicago/Turabian StyleOrlane Anneville; Chun‐Wei Chang; Gaël Dur; Sami Souissi; Frédéric Rimet; Chih‐Hao Hsieh. 2019. "The paradox of re‐oligotrophication: the role of bottom–up versus top–down controls on the phytoplankton community." Oikos 128, no. 11: 1666-1677.
We demonstrated that oral intake of water by two calanoid copepods, Pseudodiaptomus annandalei and Eurytemora affinis takes place and has implications for their ecotoxicology. In the first experiment, copepods were exposed to a dyed medium, which allowed us to visually examine the possibility of water uptake by the copepod. We observed that both copepod species were taking in water orally and evacuated dye at different speeds. This exposure left concentrated dye in the guts of the copepods indicating adsorption into the gut epithelium. This was further demonstrated by exposing both copepod species independently to dissolved metals (Cd,17 µg/L; Cu,13.8 µg/L; Ni, 29.3 µg/L) and to dietary metals (Cd,18.8 µg/g; Cu, 35.3 µg/g; Ni, 32.5 µg/g). The results showed that although the concentration of dissolved metals they were exposed to were lower than those of the dietary metals, nevertheless, uptake of metals by both copepod species from the dissolved phase alone was substantially higher than from dietary exposure. This provides clear evidence to support our hypothesis that higher metal body burden observed in copepods exposed to dissolved metals than in those exposed to dietary metals is an implication of oral intake of water. P. annandalei showed higher excretion rate of metals when exposed to dissolved metals than E. affinis. However, the excretion rate of metals from both copepod species exposed to dietary metal was similar. We conclude here that both copepod species take in water orally. Our study further showed that metal uptake depends on the exposure routes and the uptake and excretion rates are dependent on the type of metals, amounts and the species.
Esther U. Kadiene; Baghdad Ouddane; Jiang-Shiou Hwang; Sami Souissi. Bioaccumulation of metals in calanoid copepods by oral intake. Scientific Reports 2019, 9, 1 -9.
AMA StyleEsther U. Kadiene, Baghdad Ouddane, Jiang-Shiou Hwang, Sami Souissi. Bioaccumulation of metals in calanoid copepods by oral intake. Scientific Reports. 2019; 9 (1):1-9.
Chicago/Turabian StyleEsther U. Kadiene; Baghdad Ouddane; Jiang-Shiou Hwang; Sami Souissi. 2019. "Bioaccumulation of metals in calanoid copepods by oral intake." Scientific Reports 9, no. 1: 1-9.
The fate and the accumulation kinetics of mercurychloride (HgCl2) were investigated in the invasive copepod species Pseudodiaptomus marinus, which originates from the North-Western Pacific Ocean and was recently recorded from the Atlantic and Mediterranean coasts. The main objective of this study was to determine lethal concentrations (LC50 %) of HgCl2 in P. marinus and to study its bioaccumulation kinetics in the laboratory. Lethality experiments were performed for 24, 48, 72, and 96 h. Experiments in presence and absence of food source using one sub-lethal concentration of HgCl2 (14.15 μg/L) were carried out to study the uptake, the accumulation and the influence of exposure pathways of HgCl2 in P. marinus. LC50 for 96 h was calculated as 42.4 μg/L in response to HgCl2. The uptake and bioaccumulation kinetics of HgCl2 in P. marinus are not depending on the exposure pathways, where no significant differences were depicted between the uptake/accumulation of HgCl2 from the micro-algal diet and from the seawater medium. Those results could be helpful in the understanding of mercury uptake, bioaccumulation and bio-amplification processes especially concerning invasive copepod species.
Sofiene Tlili; Julien Ovaert; Anissa Souissi; Baghdad Ouddane; Jae-Seong Lee; Sami Souissi. Bioaccumulation of Mercury in the Copepod Pseudodiaptomus marinus: A Comparative Study Between Waterborne and Dietary Pathways. International Journal of Environmental Research 2019, 13, 759 -768.
AMA StyleSofiene Tlili, Julien Ovaert, Anissa Souissi, Baghdad Ouddane, Jae-Seong Lee, Sami Souissi. Bioaccumulation of Mercury in the Copepod Pseudodiaptomus marinus: A Comparative Study Between Waterborne and Dietary Pathways. International Journal of Environmental Research. 2019; 13 (4):759-768.
Chicago/Turabian StyleSofiene Tlili; Julien Ovaert; Anissa Souissi; Baghdad Ouddane; Jae-Seong Lee; Sami Souissi. 2019. "Bioaccumulation of Mercury in the Copepod Pseudodiaptomus marinus: A Comparative Study Between Waterborne and Dietary Pathways." International Journal of Environmental Research 13, no. 4: 759-768.
We determined the effect of acute and chronic toxicity of cadmium (Cd) on Pseudodiaptomus annandalei, according to their developmental and reproductive stages. Firstly, to estimate the 50% lethal concentration (LC50), acute exposure of nauplii and copepodids to 20, 40, 80, 150, and 300 μg/L of Cd was tested, and the effects of 5 μg/L and 40 μg/L of Cd on copepod developmental rate was done. Female lifespan and number of nauplii produced were compared. Secondly, one generation of copepod was exposed to dissolved (WCd) and dietary (DCd) Cd, and sex-specific Cd uptake and population density were estimated. 96 h LC50 was 40 μg/L Cd for nauplii and 120 μg/L Cd for copepodids. Duration of copepod development was 3.5 days and 5.5 days longer than the control when exposed to 5 μg/L and 40 μg/L of Cd, respectively. Female lifespan in both treatments were 9 and 8 days shorter than in the control, respectively. Total number of nauplii produced per female lifespan was 440 (control), 450 (5 μg/L Cd), and 365 (40 μg/L Cd). Cd uptake in copepods increased from nauplii to adults when exposed to dietary Cd and decreased when exposed to dissolved Cd. Dietary uptake of Cd was significantly higher in males than in females and Cd uptake from water was higher in males than in females, but not statistically significant. The total population of copepods were significantly affected by Cd. The toxic effects of Cd in copepods appear to depend on developmental stage, sex, duration and uptake route.
Esther U. Kadiene; Pei-Jie Meng; Jiang-Shiou Hwang; Sami Souissi. Acute and chronic toxicity of cadmium on the copepod Pseudodiaptomus annandalei: A life history traits approach. Chemosphere 2019, 233, 396 -404.
AMA StyleEsther U. Kadiene, Pei-Jie Meng, Jiang-Shiou Hwang, Sami Souissi. Acute and chronic toxicity of cadmium on the copepod Pseudodiaptomus annandalei: A life history traits approach. Chemosphere. 2019; 233 ():396-404.
Chicago/Turabian StyleEsther U. Kadiene; Pei-Jie Meng; Jiang-Shiou Hwang; Sami Souissi. 2019. "Acute and chronic toxicity of cadmium on the copepod Pseudodiaptomus annandalei: A life history traits approach." Chemosphere 233, no. : 396-404.
The trace metal cadmium (Cd) is a toxic pollutant known to induce oxidative stress and other sublethal to lethal effects on aquatic organisms. We exposed the marine mud shrimp Austinogebia edulis to Cd concentrations of 0, 0.5, and 5 mg/kg for up to 4 days (24, 48, 72, 96 h). We studied the activity of antioxidant enzymes in the hepatopancreas, gill, and muscle of A. edulis. Antioxidant enzymes (SOD, CAT, and GPx) decreased with increasing Cd concentration and extended exposure time in these three organs of the mud shrimp A. edulis. Increasing Cd concentration led to an increase in ROS and resulted ultimately in membrane lipid peroxidation at higher Cd concentrations. Significant damage of the hepatopancreas of A. edulis was noticed at higher concentrations of Cd, showing damages like the disappearance of epithelial cell boundaries, detachment of cells from the basal lamina, cellular swelling, necrosis, and reduction of glycogen. In conclusion, Cd caused oxidative damage by reducing the activities of antioxidant enzymes and by damaging the tissue structure in major organs of the mud shrimp A. edulis.
Shagnika Das; Li-Chun Tseng; Chi Chou; Lan Wang; Sami Souissi; Jiang-Shiou Hwang. Effects of cadmium exposure on antioxidant enzymes and histological changes in the mud shrimp Austinogebia edulis (Crustacea: Decapoda). Environmental Science and Pollution Research 2019, 26, 7752 -7762.
AMA StyleShagnika Das, Li-Chun Tseng, Chi Chou, Lan Wang, Sami Souissi, Jiang-Shiou Hwang. Effects of cadmium exposure on antioxidant enzymes and histological changes in the mud shrimp Austinogebia edulis (Crustacea: Decapoda). Environmental Science and Pollution Research. 2019; 26 (8):7752-7762.
Chicago/Turabian StyleShagnika Das; Li-Chun Tseng; Chi Chou; Lan Wang; Sami Souissi; Jiang-Shiou Hwang. 2019. "Effects of cadmium exposure on antioxidant enzymes and histological changes in the mud shrimp Austinogebia edulis (Crustacea: Decapoda)." Environmental Science and Pollution Research 26, no. 8: 7752-7762.
Yen‐Ju Pan; Anissa Souissi; Irina Sadovskaya; Jiang‐Shiou Hwang; Sami Souissi. Cover Image. Aquaculture Research 2019, 50, 1 .
AMA StyleYen‐Ju Pan, Anissa Souissi, Irina Sadovskaya, Jiang‐Shiou Hwang, Sami Souissi. Cover Image. Aquaculture Research. 2019; 50 (2):1.
Chicago/Turabian StyleYen‐Ju Pan; Anissa Souissi; Irina Sadovskaya; Jiang‐Shiou Hwang; Sami Souissi. 2019. "Cover Image." Aquaculture Research 50, no. 2: 1.
We evaluated the acute toxicities of metals cadmium (Cd), copper (Cu) and nickel (Ni) to a widely-distributed copepod Eurytemora affinis isolated from the Seine estuary. Both sexes of adult E. affinis were exposed separately to the three metals at concentration gradients to determine its 50% lethal concentration (LC50). After 4 days of exposure, both males and females showed a higher sensitivity to Cu (male LC50: 25.0 μg.L-1 and female LC50: 38.0 μg.L-1) than to Ni (male LC50: 90.0 μg.L-1 and female 161.0 μg.L-1) and Cd (male LC50: 127.8 μg.L-1 and female LC50: 90.0 μg.L-1). To assess for the first time, the extend of metal bioaccumulation and its effect at population scale, late stages (> 200 μm) were collected and exposed to each metal at the concentration of 1/3 LC50, and to their mixture during 144h without feeding. The Cd concentration consistently increased with time until the end of the experiment, whereas the Ni and Cu concentrations reached a plateau after 24h and 72h exposure, respectively. The results revealed that the copepods could accumulate Cu faster than Ni and Cd either in the treatment alone (0.58 L.g-1.d-1) or in the three-metal mixture (0.72 L.g-1.d-1) after 50% of exposure time (72h). The number of individuals decreased in copepod populations except for the Cd treatment, where the number of nauplii increased. In addition, all treatments of metal exposure negatively affected bacterial densities in the copepod cultures, where the Cu treatment showed a negative remarkable effect compared with Cd and Ni treatment did.
Mahammed Zidour; Zakia Boubechiche; Yen-Ju Pan; Capucine Bialais; Benoit Cudennec; Thierry Grard; Djamel Drider; Christophe Flahaut; Baghdad Ouddane; Sami Souissi. Population response of the estuarine copepod Eurytemora affinis to its bioaccumulation of trace metals. Chemosphere 2018, 220, 505 -513.
AMA StyleMahammed Zidour, Zakia Boubechiche, Yen-Ju Pan, Capucine Bialais, Benoit Cudennec, Thierry Grard, Djamel Drider, Christophe Flahaut, Baghdad Ouddane, Sami Souissi. Population response of the estuarine copepod Eurytemora affinis to its bioaccumulation of trace metals. Chemosphere. 2018; 220 ():505-513.
Chicago/Turabian StyleMahammed Zidour; Zakia Boubechiche; Yen-Ju Pan; Capucine Bialais; Benoit Cudennec; Thierry Grard; Djamel Drider; Christophe Flahaut; Baghdad Ouddane; Sami Souissi. 2018. "Population response of the estuarine copepod Eurytemora affinis to its bioaccumulation of trace metals." Chemosphere 220, no. : 505-513.
To investigate egg storage capacity of the copepod Acartia bilobata for aquaculture interest, we tested hatching success rate (HSR) of inclusive eggs (mixture of all egg types) after 4°C storage. The HSR peaked after 14 days storage when incubating at 28°C for 48 hr (85.8 ± 1.6%) and 72 hr (87.6 ± 0.9%), then gradually declined until 1 year (48 hr: 7 ± 0.6%; 72 hr: 19.4 ± 3.9%). Reallocation of fatty acid profile suggests that docosahexaenoic acid (DHA) is correlated with the HSR of A. bilobata eggs. Additionally, we investigated the HSR of diapausing eggs (unhatched eggs after 72 hr incubation of the inclusive eggs) after 4°C storage. Their HSR peaked after 14 days storage (48 hr:75.3 ± 3.5%; 72 hr:78.2 ± 2.1%), then gradually declined until 60 days (48 hr HSR:42.1 ± 2.3%; 72 hr HSR:53.0 ± 3.2%). Overall, we illustrated the hatchability of diapausing and quiescent eggs of A. bilobata after 4°C storage. The cold storage capacities were low (<60% HSR after 60 days), and it could be limited by the egg DHA content. Our findings provide implications for future studies aiming to improve cold storage techniques of tropical copepod eggs for aquaculture applications.
Yen-Ju Pan; Anissa Souissi; Irina Sadovskaya; Jiang‐Shiou Hwang; Sami Souissi. Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations. Aquaculture Research 2018, 50, 483 -489.
AMA StyleYen-Ju Pan, Anissa Souissi, Irina Sadovskaya, Jiang‐Shiou Hwang, Sami Souissi. Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations. Aquaculture Research. 2018; 50 (2):483-489.
Chicago/Turabian StyleYen-Ju Pan; Anissa Souissi; Irina Sadovskaya; Jiang‐Shiou Hwang; Sami Souissi. 2018. "Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations." Aquaculture Research 50, no. 2: 483-489.
The invasion of exotic species into native ecosystems is becoming a crucial issue in global biology. Over the last ten years, at least 45 invasions of aquatic species have been reported in the eastern part of the Gulf of Finland; the majority of them were introduced through ballast water. Recently, invasion of the estuarine calanoid copepod Eurytemora carolleeae (Temoridae), originating from North America, has been reported in several European estuaries and particularly in the Gulf of Finland. This species is morphologically very similar to the native Eurytemora affinis, but it is easily discriminated by molecular markers. In this study, we monitored the distribution area of the invasive copepod species in European waters, as well as the population structure of (native) E. affinis and (invasive) E. carolleeae, from 2006 to 2018 in the Gulf of Finland. The population density of E. affinis was significantly higher, compared to E. carolleeae, during most of the study period. The only exception was the Neva Bay in 2010, wherein the invasive species dominated possibly due to high temperatures and differences in the levels of fish predation. The reproductive performance of E. carolleeae was also higher than that of E. affinis. These results show different population dynamics between the two species. It was revealed that invasive E. carolleeae develops in some of the very same habitats as native E. affinis, thereby potentially becoming a significant component of the zooplankton in the studied area. Moreover, invader has the potential to displace native E. affinis.
Natalia Sukhikh; Anissa Souissi; Sami Souissi; Anne-Catherine Holl; Nikolaos V. Schizas; Victor Alekseev. Life in sympatry: coexistence of native Eurytemora affinis and invasive Eurytemora carolleeae in the Gulf of Finland (Baltic Sea). Oceanologia 2018, 61, 227 -238.
AMA StyleNatalia Sukhikh, Anissa Souissi, Sami Souissi, Anne-Catherine Holl, Nikolaos V. Schizas, Victor Alekseev. Life in sympatry: coexistence of native Eurytemora affinis and invasive Eurytemora carolleeae in the Gulf of Finland (Baltic Sea). Oceanologia. 2018; 61 (2):227-238.
Chicago/Turabian StyleNatalia Sukhikh; Anissa Souissi; Sami Souissi; Anne-Catherine Holl; Nikolaos V. Schizas; Victor Alekseev. 2018. "Life in sympatry: coexistence of native Eurytemora affinis and invasive Eurytemora carolleeae in the Gulf of Finland (Baltic Sea)." Oceanologia 61, no. 2: 227-238.
Here we show that Bacillus pumilus ICVB403 recently isolated from copepod eggs is able to produce, after 48–72 h of growth in Landy medium, extracellular inhibitory compounds, which are active against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, MRSA-S1, Staphylococcus epidermidis 11EMB, Staphylococcus warneri 27EMB, and Staphylococcus hominis 13EMB. Moreover, these extracellular inhibitory compound(s) were able to potentiate erythromycin against the aforementioned staphylococci. The minimum inhibitory concentration (MIC) of erythromycin was reduced from 32 μg/mL to 8 μg/mL for MRSA ATCC 43300 and MRSA SA-1 strains, and from 32–64 μg/mL to 4 μg/mL for S. epidermidis 11EMB and S. hominis 13EMB strains. The genome sequencing and analysis of B. pumilus ICVB403 unveiled 3.666.195 nucleotides contained in 22 contigs with a G + C ratio of 42.0%, 3.826 coding sequences, and 73 RNAs. In silico analysis guided identification of two putative genes coding for synthesis of surfactin A, a lipopeptide with 7 amino acids, and for a circular bacteriocin belonging to the circularin A/uberolysin family, respectively.
Mahammed Zidour; Yanath Belguesmia; Benoit Cudennec; Thierry Grard; Christophe Flahaut; Sami Souissi; Djamel Drider. Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity. Probiotics and Antimicrobial Proteins 2018, 11, 990 -998.
AMA StyleMahammed Zidour, Yanath Belguesmia, Benoit Cudennec, Thierry Grard, Christophe Flahaut, Sami Souissi, Djamel Drider. Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity. Probiotics and Antimicrobial Proteins. 2018; 11 (3):990-998.
Chicago/Turabian StyleMahammed Zidour; Yanath Belguesmia; Benoit Cudennec; Thierry Grard; Christophe Flahaut; Sami Souissi; Djamel Drider. 2018. "Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity." Probiotics and Antimicrobial Proteins 11, no. 3: 990-998.