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No effective cryopreservation technique exists for fish eggs and embryos; thus, the cryopreservation of germ cells (spermatogonia or oogonia) and subsequent generation of eggs and sperm would be an alternative solution for the long-term preservation of piscine genetic resources. Nevertheless, in our previous study using rainbow trout, we showed that recipients transplanted with XY spermatogonia or XX oogonia produced unnatural sex-biased F1 offspring. To overcome these obstacles, we transplanted immature germ cells (XX oogonia or XY spermatogonia; frozen for 33 days) into the body cavities of triploid hatchlings, and the transplanted germ cells possessed a high capacity for differentiating into eggs and sperm in the ovaries and testes of recipients. Approximately 30% of triploid recipients receiving frozen germ cells generated normal salmon that displayed the donor-derived black body color phenotype, although all triploid salmon not receiving transplants were functionally sterile. Furthermore, F1 offspring obtained from insemination of the oogonia-derived eggs and spermatogonia-derived sperm show a normal sex ratio of 1:1 (female:male). Thus, this method presented a critical technique for practical conservation projects for other teleost fish species and masu salmon.
Seungki Lee; Woo Young Bang; Hee-Sun Yang; Dae-Sung Lee; Ha Yeun Song. Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells. Biochemical and Biophysical Research Communications 2020, 535, 6 -11.
AMA StyleSeungki Lee, Woo Young Bang, Hee-Sun Yang, Dae-Sung Lee, Ha Yeun Song. Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells. Biochemical and Biophysical Research Communications. 2020; 535 ():6-11.
Chicago/Turabian StyleSeungki Lee; Woo Young Bang; Hee-Sun Yang; Dae-Sung Lee; Ha Yeun Song. 2020. "Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells." Biochemical and Biophysical Research Communications 535, no. : 6-11.
The recent pandemic of coronavirus disease 2019 (COVID-19) has increased demand for chemical disinfectants, which can be potentially hazardous to users. Here, we suggest that the cell-free supernatant from Lactobacillus plantarum NIBR97, including novel bacteriocins, has potential as a natural alternative to chemical disinfectants. It exhibits significant antibacterial activities against a broad range of pathogens, and was observed by scanning electron microscopy (SEM) to cause cellular lysis through pore formation in bacterial membranes, implying that its antibacterial activity may be mediated by peptides or proteins and supported by proteinase K treatment. It also showed significant antiviral activities against HIV-based lentivirus and influenza A/H3N2, causing lentiviral lysis through envelope collapse. Furthermore, whole-genome sequencing revealed that NIBR97 has diverse antimicrobial peptides, and among them are five novel bacteriocins, designated as plantaricin 1 to 5. Plantaricin 3 and 5 in particular showed both antibacterial and antiviral activities. SEM revealed that plantaricin 3 causes direct damage to both bacterial membranes and viral envelopes, while plantaricin 5 damaged only bacterial membranes, implying different antiviral mechanisms. Our data suggest that the cell-free supernatant from L. plantarum NIBR97, including novel bacteriocins, is potentially useful as a natural alternative to chemical disinfectants.
Sam Kim; Song Kang; Da Shin; Se Oh; Chae Lee; Yoonyong Yang; Youn Son; Hee-Sun Yang; Byoung-Hee Lee; Hee-Jung An; In Jeong; Woo Bang. Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants. Pharmaceuticals 2020, 13, 266 .
AMA StyleSam Kim, Song Kang, Da Shin, Se Oh, Chae Lee, Yoonyong Yang, Youn Son, Hee-Sun Yang, Byoung-Hee Lee, Hee-Jung An, In Jeong, Woo Bang. Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants. Pharmaceuticals. 2020; 13 (10):266.
Chicago/Turabian StyleSam Kim; Song Kang; Da Shin; Se Oh; Chae Lee; Yoonyong Yang; Youn Son; Hee-Sun Yang; Byoung-Hee Lee; Hee-Jung An; In Jeong; Woo Bang. 2020. "Potential of Cell-Free Supernatant from Lactobacillus plantarum NIBR97, Including Novel Bacteriocins, as a Natural Alternative to Chemical Disinfectants." Pharmaceuticals 13, no. 10: 266.
The prevalence of antibiotic-resistant bacteria has become an immediate threat to public health. Antimicrobial peptides are attracting attention as a new source of antibiotics due to their ability to prevent drug-resistances with fewer side effects. Spider venom is composed of various bioactive substances with multiple functionalities such as antimicrobial and anti-inflammatory effects. Here, RNA sequencing was conducted on the venom gland of the spider Pardosa astrigera, and a potential toxin peptide with antibacterial properties was selected via homology and in silico analysis. A novel toxin, Lycotoxin-Pa4a, inhibited both gram-negative and gram-positive bacteria by disrupting the outer and bacterial cytoplasmic membrane. Moreover, the peptide downregulated the expression of pro-inflammatory mediators while upregulating the level of anti-inflammatory cytokine by inactivating mitogen-activated protein kinase signaling in a lipopolysaccharide-stimulated murine macrophage cell line. In this research, we identified a novel peptide toxin, Lycotoxin-pa4a, with antibacterial and anti-inflammatory properties, suggesting its potential for the development of a new antibiotics, as well as offering insights into the utilization of biological resources.
Min Shin; In-Wook Hwang; YunKyung Kim; Seung Kim; Wonhee Jang; Seungki Lee; Woo Bang; Chang-Hwan Bae; Jung-Suk Sung. Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera. Antibiotics 2020, 9, 422 .
AMA StyleMin Shin, In-Wook Hwang, YunKyung Kim, Seung Kim, Wonhee Jang, Seungki Lee, Woo Bang, Chang-Hwan Bae, Jung-Suk Sung. Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera. Antibiotics. 2020; 9 (7):422.
Chicago/Turabian StyleMin Shin; In-Wook Hwang; YunKyung Kim; Seung Kim; Wonhee Jang; Seungki Lee; Woo Bang; Chang-Hwan Bae; Jung-Suk Sung. 2020. "Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera." Antibiotics 9, no. 7: 422.
Phenol and formalin are major water pollutants that are frequently discharged into the aquatic milieu. These chemicals can affect broad domains of life, including microorganisms. Aquatic pollutants, unlike terrestrial pollutants, are easily diluted in water environments and exist at a sub-inhibitory concentration (sub-IC), thus not directly inhibiting bacterial growth. However, they can modulate gene expression profiles. The sub-IC values of phenol and formalin were measured by minimal inhibitory concentration (MIC) assay to be 0.146% (1.3 mM) and 0.0039% (0.38 mM), respectively, in Edwardsiella piscicida CK108, a Gram-negative fish pathogen. We investigated the differentially expressed genes (DEG) by RNA-seq when the cells were exposed to the sub-ICs of phenol and formalin. DEG analyses revealed that genes involved in major virulence factors (type I fimbriae, flagella, type III and type VI secretion system) and various cellular pathways (energy production, amino acid synthesis, carbohydrate metabolism and two-component regulatory systems) were up- or downregulated by both chemicals. The genome-wide gene expression data corresponded to the results of a quantitative reverse complementary-PCR and motility assay. This study not only provides insight into how a representative fish pathogen, E. piscicida CK108, responds to the sub-ICs of phenol and formalin but also shows the importance of controlling chemical pollutants in aquatic environments.
Ju Bin Yoon; Sungmin Hwang; Se-Won Baek; Seungki Lee; Woo Young Bang; Ki Hwan Moon. In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms. Microorganisms 2020, 8, 1068 .
AMA StyleJu Bin Yoon, Sungmin Hwang, Se-Won Baek, Seungki Lee, Woo Young Bang, Ki Hwan Moon. In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms. Microorganisms. 2020; 8 (7):1068.
Chicago/Turabian StyleJu Bin Yoon; Sungmin Hwang; Se-Won Baek; Seungki Lee; Woo Young Bang; Ki Hwan Moon. 2020. "In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms." Microorganisms 8, no. 7: 1068.
Bacteriocins are functionally diverse toxins produced by most microbes and are potent antimicrobial peptides (AMPs) for bacterial ghosts as next generation vaccines. Here, we first report that the AMPs secreted from Lactobacillus taiwanensis effectively form ghosts of pathogenic bacteria and are identified as diverse bacteriocins, including novel ones. In detail, a cell-free supernatant from L. taiwanensis exhibited antimicrobial activities against pathogenic bacteria and was observed to effectively cause cellular lysis through pore formation in the bacterial membrane using scanning electron microscopy (SEM). The treatment of the cell-free supernatant with proteinase K or EDTA proved that the antimicrobial activity is mediated by AMPs, and the purification of AMPs using Sep-Pak columns indicated that the cell-free supernatant includes various amphipathic peptides responsible for the antimicrobial activity. Furthermore, the whole-genome sequencing of L. taiwanensis revealed that the strain has diverse bacteriocins, confirmed experimentally to function as AMPs, and among them are three novel bacteriocins, designated as Tan 1, Tan 2, and Tan 3. We also confirmed, using SEM, that Tan 2 effectively produces bacterial ghosts. Therefore, our data suggest that the bacteriocins from L. taiwanensis are potentially useful as a critical component for the preparation of bacterial ghosts.
Sam Kim; Yeon Ha; Kyu Bang; Seungki Lee; Joo-Hong Yeo; Hee-Sun Yang; Tae-Won Kim; Kyu Lee; Woo Bang. Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine. Toxins 2020, 12, 432 .
AMA StyleSam Kim, Yeon Ha, Kyu Bang, Seungki Lee, Joo-Hong Yeo, Hee-Sun Yang, Tae-Won Kim, Kyu Lee, Woo Bang. Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine. Toxins. 2020; 12 (7):432.
Chicago/Turabian StyleSam Kim; Yeon Ha; Kyu Bang; Seungki Lee; Joo-Hong Yeo; Hee-Sun Yang; Tae-Won Kim; Kyu Lee; Woo Bang. 2020. "Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine." Toxins 12, no. 7: 432.
Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed bimonthly journal publishing original research and reviews on basic and applied aspects of the use of animal resources including the meat, egg, dairy, and edible insect/worm products. Food Sci. Anim. Resour. is published and distributed by the Korean Society for Food Science of Animal Resources.
Chul-Ho Yoon; Seung-Ho Jeon; Yeon Jo Ha; Sam Woong Kim; Woo Young Bang; Kyu Ho Bang; Sang Wan Gal; Il-Suk Kim; Young-Son Cho. Functional Chemical Components in Protaetia brevitarsis Larvae: Impact of Supplementary Feeds. Food Science of Animal Resources 2020, 40, 461 -473.
AMA StyleChul-Ho Yoon, Seung-Ho Jeon, Yeon Jo Ha, Sam Woong Kim, Woo Young Bang, Kyu Ho Bang, Sang Wan Gal, Il-Suk Kim, Young-Son Cho. Functional Chemical Components in Protaetia brevitarsis Larvae: Impact of Supplementary Feeds. Food Science of Animal Resources. 2020; 40 (3):461-473.
Chicago/Turabian StyleChul-Ho Yoon; Seung-Ho Jeon; Yeon Jo Ha; Sam Woong Kim; Woo Young Bang; Kyu Ho Bang; Sang Wan Gal; Il-Suk Kim; Young-Son Cho. 2020. "Functional Chemical Components in Protaetia brevitarsis Larvae: Impact of Supplementary Feeds." Food Science of Animal Resources 40, no. 3: 461-473.
Edwardsiella piscicida CK41 is a fish-pathogenic Gram-negative bacterium isolated from diseased flounder in the Republic of Korea. Here, we report the genome sequence of E. piscicida CK41, comprising one chromosome of 3.76 Mbp and one plasmid of 72.7 kbp. A total of 3,406 protein-coding genes, 98 tRNAs, and 25 rRNAs are predicted to be present in the genome.
Se-Won Baek; Sungmin Hwang; Ho Young Kang; Woo Young Bang; Ki Hwan Moon. Draft Genome Sequence of a Fish Pathogen, Edwardsiella piscicida Isolate CK41. Microbiology Resource Announcements 2020, 9, 1 .
AMA StyleSe-Won Baek, Sungmin Hwang, Ho Young Kang, Woo Young Bang, Ki Hwan Moon. Draft Genome Sequence of a Fish Pathogen, Edwardsiella piscicida Isolate CK41. Microbiology Resource Announcements. 2020; 9 (17):1.
Chicago/Turabian StyleSe-Won Baek; Sungmin Hwang; Ho Young Kang; Woo Young Bang; Ki Hwan Moon. 2020. "Draft Genome Sequence of a Fish Pathogen, Edwardsiella piscicida Isolate CK41." Microbiology Resource Announcements 9, no. 17: 1.
To identify and compare venom components and expression patterns, venom gland-specific transcriptome analyses were conducted for 14 Aculeate bees and wasps. TPM (transcripts per kilobase million) values were normalized using the average transcription level of a reference housekeeping gene (dimethyladenosine transferase). Orthologous venom component genes across the 14 bee and wasp species were identified, and their relative abundance in each species was determined by comparing normalized TPM values. Based on signal sequences in the transcripts, the genes of novel venom components were identified and characterized to encode potential allergens. Most of the allergens and pain-producing factors (arginine kinase, hyaluronidase, mastoparan, phospholipase A1, phospholipase A2, and venom allergen 5) showed extremely high expression levels in social wasps. Acid phosphatase, neprilysin, and tachykinin, which are known allergens and neurotoxic peptides, were found in the venom glands of solitary wasps more often than in social wasps. In the venom glands of bumblebees, few or no transcripts of major allergens or pain-producing factors were identified. Taken together, these results indicate that differential expression patterns of the venom genes in some Aculeate species imply that some wasps and bumblebee species have unique groups of highly expressed venom components. Some venom components reflected the Aculeate species phylogeny, but others did not. This unique evolution of specific venom components in different groups of some wasps and bumblebee species might have been shaped in response to both ecological and behavioral influences.
Kyungjae Andrew Yoon; Kyungmun Kim; Woo-Jin Kim; Woo Young Bang; Neung-Ho Ahn; Chang-Hwan Bae; Joo-Hong Yeo; Si Hyeock Lee; Kim. Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps. Toxins 2020, 12, 47 .
AMA StyleKyungjae Andrew Yoon, Kyungmun Kim, Woo-Jin Kim, Woo Young Bang, Neung-Ho Ahn, Chang-Hwan Bae, Joo-Hong Yeo, Si Hyeock Lee, Kim. Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps. Toxins. 2020; 12 (1):47.
Chicago/Turabian StyleKyungjae Andrew Yoon; Kyungmun Kim; Woo-Jin Kim; Woo Young Bang; Neung-Ho Ahn; Chang-Hwan Bae; Joo-Hong Yeo; Si Hyeock Lee; Kim. 2020. "Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps." Toxins 12, no. 1: 47.
Hyperglycemia-induced oxidative stress triggers severe vascular damage and induces an inflammatory vascular state, and is, therefore, one of the main causes of atherosclerosis. Recently, interest in the natural compound Carpinus turczaninowii has increased because of its reported antioxidant and anti-inflammatory properties. We investigated whether a C. turczaninowii extract was capable of attenuating high glucose-induced inflammation and arterial damage using human aortic vascular smooth muscle cells (hASMCs). mRNA expression levels of proinflammatory response [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α)], endoplasmic reticulum (ER) stress [CCAAT-enhancer-binding proteins (C/EBP) homologous protein (CHOP)], and adenosine monophosphate (AMP)-protein activated kinase α2 (AMPK α2)], and DNA damage [phosphorylated H2.AX (p-H2.AX)] were measured in hASMCs treated with the C. turczaninowii extracts (1 and 10 μg/mL) after being stimulated by high glucose (25 mM) or not. The C. turczaninowii extract attenuated the increased mRNA expression of IL-6, TNF-α, and CHOP in hASMCs under high glucose conditions. The expression levels of p-H2.AX and AMPK α2 induced by high glucose were also significantly decreased in response to treatment with the C. turczaninowii extract. In addition, 15 types of phenolic compounds including quercetin, myricitrin, and ellagic acid, which exhibit antioxidant and anti-inflammatory properties, were identified in the C. turczaninowii extract through ultra-performance liquid chromatography-quadrupole-time of flight (UPLC-Q-TOF) mass spectrometry. In conclusion, C. turczaninowii may alleviate high glucose-induced inflammation and arterial damage in hASMCs, and may have potential in the treatment of hyperglycemia-induced atherosclerosis.
Juhyun Song; So Ra Yoon; Youn Kyoung Son; Woo Young Bang; Chang-Hwan Bae; Joo-Hong Yeo; Hyun-Jin Kim; Oh Yoen Kim. Carpinus turczaninowii Extract May Alleviate High Glucose-Induced Arterial Damage and Inflammation. Antioxidants 2019, 8, 172 .
AMA StyleJuhyun Song, So Ra Yoon, Youn Kyoung Son, Woo Young Bang, Chang-Hwan Bae, Joo-Hong Yeo, Hyun-Jin Kim, Oh Yoen Kim. Carpinus turczaninowii Extract May Alleviate High Glucose-Induced Arterial Damage and Inflammation. Antioxidants. 2019; 8 (6):172.
Chicago/Turabian StyleJuhyun Song; So Ra Yoon; Youn Kyoung Son; Woo Young Bang; Chang-Hwan Bae; Joo-Hong Yeo; Hyun-Jin Kim; Oh Yoen Kim. 2019. "Carpinus turczaninowii Extract May Alleviate High Glucose-Induced Arterial Damage and Inflammation." Antioxidants 8, no. 6: 172.
Vascular inflammation is an important feature in the atherosclerotic process. Recent studies report that leaves and branches of Carpinus turczaninowii (C. turczaninowii) have antioxidant capacity and exert anti-inflammatory effects. However, no study has reported the regulatory effect of C. turczaninowii extract on the arterial inflammatory response. This study therefore investigated modulation of the arterial inflammatory response after exposure to C. turczaninowii extract, using human aortic vascular smooth muscle cells (HAoSMCs). Scavenging activity of free radicals, total phenolic content (TPC), cell viability, mRNA expressions, and secreted levels of cytokines were measured in LPS-stimulated (10 ng/mL) HAoSMCs treated with the C. turczaninowii extract. C. turczaninowii extract contains high amounts of TPC (225.6 ± 21.0 mg of gallic acid equivalents/g of the extract), as well as exerts time-and dose-dependent increases in strongly scavenged free radicals (average 14.8 ± 1.97 µg/mL IC50 at 40 min). Cell viabilities after exposure to the extracts (1 and 10 µg/mL) were similar to the viability of non-treated cells. Cytokine mRNA expressions were significantly suppressed by the extracts (1 and 10 µg/mL) at 6 hours (h) after exposure. Interleukin-6 secretion was dose-dependently suppressed 2 h after incubation with the extract, at 1-10 µg/mL in non-stimulated cells, and at 5 and 10 µg/mL in LPS-stimulated cells. Similar patterns were also observed at 24 h after incubation with the extract (at 1-10 µg/mL in non-stimulated cells, and at 10 µg/mL in the LPS-stimulated cells). Soluble intracellular vascular adhesion molecules (sICAM-1) secreted from non-stimulated cells and LPS-stimulated cells were similarly suppressed in a dose-dependent manner after 24 h exposure to the extracts, but not after 2 h. In addition, sICAM-1 concentration after 24 h treatment was positively related to IL-6 levels after 2 h and 24 h exposure (r = 0.418, P = 0.003, and r = 0.524, P < 0.001, respectively). This study demonstrates that C. turczaninowii modulates the arterial inflammatory response, and indicates the potential to be applied as a therapeutic use for atherosclerosis.
Youn Kyoung Son; So Ra Yoon; Woo Young Bang; Chang-Hwan Bae; Joo-Hong Yeo; Rimkyo Yeo; Juhyun An; Juhyun Song; Oh Yoen Kim. Carpinus turczaninowii extract modulates arterial inflammatory response: a potential therapeutic use for atherosclerosis. Nutrition Research and Practice 2019, 13, 302 -309.
AMA StyleYoun Kyoung Son, So Ra Yoon, Woo Young Bang, Chang-Hwan Bae, Joo-Hong Yeo, Rimkyo Yeo, Juhyun An, Juhyun Song, Oh Yoen Kim. Carpinus turczaninowii extract modulates arterial inflammatory response: a potential therapeutic use for atherosclerosis. Nutrition Research and Practice. 2019; 13 (4):302-309.
Chicago/Turabian StyleYoun Kyoung Son; So Ra Yoon; Woo Young Bang; Chang-Hwan Bae; Joo-Hong Yeo; Rimkyo Yeo; Juhyun An; Juhyun Song; Oh Yoen Kim. 2019. "Carpinus turczaninowii extract modulates arterial inflammatory response: a potential therapeutic use for atherosclerosis." Nutrition Research and Practice 13, no. 4: 302-309.
A previous study highlighted that mastoparan V1 (MP-V1), a mastoparan from the venom of the social wasp Vespula vulgaris, is a potent antimicrobial peptide against Salmonella infection, which causes enteric diseases. However, there exist some limits for its practical application due to the loss of its activity in an increased bacterial density and the difficulty of its efficient production. In this study, we first modulated successfully the antimicrobial activity of synthetic MP-V1 against an increased Salmonella population using protease inhibitors, and developed an Escherichia coli secretion system efficiently producing active MP-V1. The protease inhibitors used, except pepstatin A, significantly increased the antimicrobial activity of the synthetic MP-V1 at minimum inhibitory concentrations (determined against 106 cfu/mL of population) against an increased population (108 cfu/mL) of three different Salmonella serotypes, Gallinarum, Typhimurium and Enteritidis. Meanwhile, the E. coli strain harboring OmpA SS::MP-V1 was identified to successfully secrete active MP-V1 into cell-free supernatant, whose antimicrobial activity disappeared in the increased population (108 cfu/mL) of Salmonella Typhimurium recovered by adding a protease inhibitor cocktail. Therefore, it has been concluded that our challenge using the E. coli secretion system with the protease inhibitors is an attractive strategy for practical application of peptide toxins, such as MP-V1.
Yeon Jo Ha; Sam Woong Kim; Chae Won Lee; Chang-Hwan Bae; Joo-Hong Yeo; Il-Suk Kim; Sang Wan Gal; Jin Hur; Ho-Kyoung Jung; Min-Ju Kim; Woo Young Bang. Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System. Toxins 2017, 9, 321 .
AMA StyleYeon Jo Ha, Sam Woong Kim, Chae Won Lee, Chang-Hwan Bae, Joo-Hong Yeo, Il-Suk Kim, Sang Wan Gal, Jin Hur, Ho-Kyoung Jung, Min-Ju Kim, Woo Young Bang. Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System. Toxins. 2017; 9 (10):321.
Chicago/Turabian StyleYeon Jo Ha; Sam Woong Kim; Chae Won Lee; Chang-Hwan Bae; Joo-Hong Yeo; Il-Suk Kim; Sang Wan Gal; Jin Hur; Ho-Kyoung Jung; Min-Ju Kim; Woo Young Bang. 2017. "Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System." Toxins 9, no. 10: 321.
Changyeol Lee; Soonok Kim; Wei Li; Sunghee Bang; Hanna Lee; Hyun-Jung Lee; Eun-Young Noh; Jung-Eun Park; Woo Young Bang; Sang Hee Shim. Bioactive secondary metabolites produced by an endophytic fungus Gaeumannomyces sp. JS0464 from a maritime halophyte Phragmites communis. The Journal of Antibiotics 2017, 70, 737 -742.
AMA StyleChangyeol Lee, Soonok Kim, Wei Li, Sunghee Bang, Hanna Lee, Hyun-Jung Lee, Eun-Young Noh, Jung-Eun Park, Woo Young Bang, Sang Hee Shim. Bioactive secondary metabolites produced by an endophytic fungus Gaeumannomyces sp. JS0464 from a maritime halophyte Phragmites communis. The Journal of Antibiotics. 2017; 70 (6):737-742.
Chicago/Turabian StyleChangyeol Lee; Soonok Kim; Wei Li; Sunghee Bang; Hanna Lee; Hyun-Jung Lee; Eun-Young Noh; Jung-Eun Park; Woo Young Bang; Sang Hee Shim. 2017. "Bioactive secondary metabolites produced by an endophytic fungus Gaeumannomyces sp. JS0464 from a maritime halophyte Phragmites communis." The Journal of Antibiotics 70, no. 6: 737-742.
There are three main dietary groups in mammals: carnivores, omnivores, and herbivores. Currently, there is limited comparative genomics insight into the evolution of dietary specializations in mammals. Due to recent advances in sequencing technologies, we were able to perform in-depth whole genome analyses of representatives of these three dietary groups. We investigated the evolution of carnivory by comparing 18 representative genomes from across Mammalia with carnivorous, omnivorous, and herbivorous dietary specializations, focusing on Felidae (domestic cat, tiger, lion, cheetah, and leopard), Hominidae, and Bovidae genomes. We generated a new high-quality leopard genome assembly, as well as two wild Amur leopard whole genomes. In addition to a clear contraction in gene families for starch and sucrose metabolism, the carnivore genomes showed evidence of shared evolutionary adaptations in genes associated with diet, muscle strength, agility, and other traits responsible for successful hunting and meat consumption. Additionally, an analysis of highly conserved regions at the family level revealed molecular signatures of dietary adaptation in each of Felidae, Hominidae, and Bovidae. However, unlike carnivores, omnivores and herbivores showed fewer shared adaptive signatures, indicating that carnivores are under strong selective pressure related to diet. Finally, felids showed recent reductions in genetic diversity associated with decreased population sizes, which may be due to the inflexible nature of their strict diet, highlighting their vulnerability and critical conservation status. Our study provides a large-scale family level comparative genomic analysis to address genomic changes associated with dietary specialization. Our genomic analyses also provide useful resources for diet-related genetic and health research.
Soonok Kim; Yun Sung Cho; Hak-Min Kim; Oksung Chung; Hyunho Kim; Sungwoong Jho; Hong Seomun; Jeongho Kim; Woo Young Bang; Changmu Kim; Junghwa An; Chang Hwan Bae; Youngjune Bhak; Sungwon Jeon; Hyejun Yoon; Yumi Kim; Jehoon Jun; Hyejin Lee; Suan Cho; Olga Uphyrkina; Aleksey Kostyria; John Goodrich; Dale Miquelle; Melody Roelke; John Lewis; Andrey Yurchenko; Anton Bankevich; Juok Cho; Semin Lee; Jeremy S. Edwards; Jessica A. Weber; Jo Cook; Sangsoo Kim; Hang Lee; Andrea Manica; Ilbeum Lee; Stephen J. O’Brien; Jong Bhak; Joo-Hong Yeo. Comparison of carnivore, omnivore, and herbivore mammalian genomes with a new leopard assembly. Genome Biology 2016, 17, 1 -12.
AMA StyleSoonok Kim, Yun Sung Cho, Hak-Min Kim, Oksung Chung, Hyunho Kim, Sungwoong Jho, Hong Seomun, Jeongho Kim, Woo Young Bang, Changmu Kim, Junghwa An, Chang Hwan Bae, Youngjune Bhak, Sungwon Jeon, Hyejun Yoon, Yumi Kim, Jehoon Jun, Hyejin Lee, Suan Cho, Olga Uphyrkina, Aleksey Kostyria, John Goodrich, Dale Miquelle, Melody Roelke, John Lewis, Andrey Yurchenko, Anton Bankevich, Juok Cho, Semin Lee, Jeremy S. Edwards, Jessica A. Weber, Jo Cook, Sangsoo Kim, Hang Lee, Andrea Manica, Ilbeum Lee, Stephen J. O’Brien, Jong Bhak, Joo-Hong Yeo. Comparison of carnivore, omnivore, and herbivore mammalian genomes with a new leopard assembly. Genome Biology. 2016; 17 (1):1-12.
Chicago/Turabian StyleSoonok Kim; Yun Sung Cho; Hak-Min Kim; Oksung Chung; Hyunho Kim; Sungwoong Jho; Hong Seomun; Jeongho Kim; Woo Young Bang; Changmu Kim; Junghwa An; Chang Hwan Bae; Youngjune Bhak; Sungwon Jeon; Hyejun Yoon; Yumi Kim; Jehoon Jun; Hyejin Lee; Suan Cho; Olga Uphyrkina; Aleksey Kostyria; John Goodrich; Dale Miquelle; Melody Roelke; John Lewis; Andrey Yurchenko; Anton Bankevich; Juok Cho; Semin Lee; Jeremy S. Edwards; Jessica A. Weber; Jo Cook; Sangsoo Kim; Hang Lee; Andrea Manica; Ilbeum Lee; Stephen J. O’Brien; Jong Bhak; Joo-Hong Yeo. 2016. "Comparison of carnivore, omnivore, and herbivore mammalian genomes with a new leopard assembly." Genome Biology 17, no. 1: 1-12.
Highlights•PvVCP only showed anti-fungal activity to Candida albicans.•PvVCP exhibited low hemolytic activity but high anti-tumor activity.•PvVCP has more amphiphilic α-helix structure than PvVespk. AbstractWe identified vespid chemotactic peptide (VCP) and vespakinin (Vespk) from the lesser paper wasp, Parapolybia varia. The cDNA, genomic DNA, and mature peptide sequences of P. varia VCP (PvVCP) and Vespk (PvVespk) were determined. To investigate the pharmacological and toxicological properties of PvVCP and PvVespk, their hemolytic, anti-microbial, anti-fungal, and anti-tumor activities were evaluated and compared with those of Vespa mandarina VCP (VmVCP) and Vespk (VmVespk). PvVCP, PvVespk, and VmVespk showed little to low hemolytic activities. Only VmVCP showed hemolytic activity at a high concentration. Among the four peptides tested, VmVCP showed both anti-microbial and anti-fungal activities, whereas PvVCP showed only anti-fungal activity to Candida albicans. Interestingly, PvVCP showed significantly stronger anti-tumor activities to two ovarian cancer cell lines compared with VmVCP. Vespks only showed anti-tumor activity to SK-OV-3 cells but not to NIH-OVCAR-3 cells. These differences in anti-tumor activity might have been caused by the differences in secondary structure among peptides. A circular dichroism spectrometry analysis revealed that VCPs have more amphiphilic α-helix structures than Vespks. Taken together, the low hemolytic but strong anti-tumor activities of PvVCP suggest that this peptide could be a candidate for developing a new anti-tumor peptide drug or drug carrier in the future. Graphical abstract
Kyungjae Andrew Yoon; Kyungmun Kim; A-Young Kim; Young Han Park; Woo Young Bang; Changmu Kim; Joo-Hong Yeo; Young Ho Koh; Si Hyeock Lee. Selective anti-tumor activities of venom peptides from the lesser paper wasp Parapolybia varia. Journal of Asia-Pacific Entomology 2016, 19, 821 -828.
AMA StyleKyungjae Andrew Yoon, Kyungmun Kim, A-Young Kim, Young Han Park, Woo Young Bang, Changmu Kim, Joo-Hong Yeo, Young Ho Koh, Si Hyeock Lee. Selective anti-tumor activities of venom peptides from the lesser paper wasp Parapolybia varia. Journal of Asia-Pacific Entomology. 2016; 19 (3):821-828.
Chicago/Turabian StyleKyungjae Andrew Yoon; Kyungmun Kim; A-Young Kim; Young Han Park; Woo Young Bang; Changmu Kim; Joo-Hong Yeo; Young Ho Koh; Si Hyeock Lee. 2016. "Selective anti-tumor activities of venom peptides from the lesser paper wasp Parapolybia varia." Journal of Asia-Pacific Entomology 19, no. 3: 821-828.
Mastoparans from the venom of social wasps have attracted considerable attention as effective antibiotic candidates. In this study, mastoparan V1 (MP-V1) from Vespula vulgaris was first disclosed to have a peptide amino acid sequence distinct from typical mastoparans and its biochemical properties and antimicrobial effects were compared with those of typical mastoparans MP-L, -X(V) and -B. Circular dichroism (CD) spectroscopy revealed that MP-V1 and -X(V) form more stable α-helical conformations in lipid membrane-like environments than MP-L and -B. In parallel, these two also showed more effective antimicrobial activities against the pathogens than did MP-L and -B. Although MP-V1 had a less stable α-helical conformation than MP-X(V), it showed stronger antimicrobial effects against Streptococcus mutans and Salmonella enterica than MP-X(V). In the meantime, analysis of hemolytic activity revealed a range of doses (~50 μM) that exhibited little potent cytotoxicity on human erythrocytes. Finally, the atypical MP-V1 peptide amino acid sequence provided important clues to understanding its antimicrobial mechanism from a structural perspective. Therefore, it has been concluded that MP-V1 is a de novo type of mastoparan with superior antimicrobial activities against both pathogenic bacteria and fungi, which may be useful in developing multipurpose antimicrobial drugs against infectious diseases.
Yangseon Kim; Minky Son; Eun-Young Noh; Soonok Kim; Changmu Kim; Joo-Hong Yeo; Chanin Park; Keun Woo Lee; Woo Young Bang. MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities. Molecules 2016, 21, 512 .
AMA StyleYangseon Kim, Minky Son, Eun-Young Noh, Soonok Kim, Changmu Kim, Joo-Hong Yeo, Chanin Park, Keun Woo Lee, Woo Young Bang. MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities. Molecules. 2016; 21 (4):512.
Chicago/Turabian StyleYangseon Kim; Minky Son; Eun-Young Noh; Soonok Kim; Changmu Kim; Joo-Hong Yeo; Chanin Park; Keun Woo Lee; Woo Young Bang. 2016. "MP-V1 from the Venom of Social Wasp Vespula vulgaris Is a de Novo Type of Mastoparan that Displays Superior Antimicrobial Activities." Molecules 21, no. 4: 512.
Pig aldo-keto reductase family 1 member C1 (AKR1C1) belongs to AKR superfamily which catalyzes the NAD(P)H-dependent reduction of various substrates including steroid hormones. Previously we have reported two paralogous pig AKR1C1s, wild-type AKR1C1 (C-type) and C-terminal-truncated AKR1C1 (T-type). Also, the C-terminal region significantly contributes to the NADPH-dependent reductase activity for 5α-DHT reduction. Molecular modeling studies combined with kinetic experiments were performed to investigate structural and enzymatic differences between wild-type AKR1C1 C-type and T-type. The results of the enzyme kinetics revealed that Vmax and kcat values of the T-type were 2.9 and 1.6 folds higher than those of the C-type. Moreover, catalytic efficiency was also 1.9 fold higher in T-type compared to C-type. Since x-ray crystal structures of pig AKR1C1 were not available, three dimensional structures of the both types of the protein were predicted using homology modeling methodology and they were used for molecular dynamics simulations. The structural comparisons between C-type and T-type showed that 5α-DHT formed strong hydrogen bonds with catalytic residues such as Tyr55 and His117 in T-type. In particular, C3 ketone group of the substrate was close to Tyr55 and NADPH in T-type. Our results showed that 5α-DHT binding in T-type was more favorable for catalytic reaction to facilitate hydride transfer from the cofactor, and were consistent with experimental results. We believe that our study provides valuable information to understand important role of C-terminal region that affects enzymatic properties for 5α-DHT, and further molecular mechanism for the enzyme kinetics of AKR1C1 proteins.
Minky Son; Chanin Park; Seul Gi Kwon; Woo Young Bang; Sam Woong Kim; Chul Wook Kim; Keun Woo Lee. Structural importance of the C-terminal region in pig aldo-keto reductase family 1 member C1 and their effects on enzymatic activity. BMC Structural Biology 2015, 15, 1 .
AMA StyleMinky Son, Chanin Park, Seul Gi Kwon, Woo Young Bang, Sam Woong Kim, Chul Wook Kim, Keun Woo Lee. Structural importance of the C-terminal region in pig aldo-keto reductase family 1 member C1 and their effects on enzymatic activity. BMC Structural Biology. 2015; 15 (1):1.
Chicago/Turabian StyleMinky Son; Chanin Park; Seul Gi Kwon; Woo Young Bang; Sam Woong Kim; Chul Wook Kim; Keun Woo Lee. 2015. "Structural importance of the C-terminal region in pig aldo-keto reductase family 1 member C1 and their effects on enzymatic activity." BMC Structural Biology 15, no. 1: 1.