Doubled haploid (DH) plant production, such as anther culture (AC), is an effective tool used in modern rice breeding programs. The improved efficient protocols applied can shorten the process of breeding. The effect of combinations of plant growth regulators (2.5 mg/L NAA, 1 mg/L 2,4-D and 0.5 mg/L kinetin; 2 mg/L 2,4-D and 0.5 mg/L BAP) in the induction medium were compared in AC for five rice breeding materials and combinations. Induction of calli ranged from 264.6 ± 67.07 to 468.8 ± 123.2 calli/100 anthers in AC of rice genotypes. Two basal media (MS and N₆) and two combinations of growth regulators (1 mg/L NAA, 1 mg/L BAP and 1 mg/L kinetin; 1.5 mg/L BAP, 0.5 mg/L NAA and 0.5 mg/L kinetin) were used as regeneration media. The in vitro green plant production was the highest with the application of the N₆NDK induction medium (NAA, 2,4-D and kinetin) and the MS-based regeneration medium (1 mg/L NAA, 1 mg/BAP and 1 mg/L kinetin) in anther culture of the ‘1009’ genotype (95.2 green plantlets/100 anthers). The mean of five genotypes was 24.48 green plantlets/100 anthers for the best treatment. Flow cytometric analyses conducted identified the microspore origin of the haploid calli produced in AC, while the uniformity of spontaneous DH plants was checked in the DH₁ and DH₂ generations. Spontaneous chromosome doubling ranged from 38.1% to 57.9% (mean 42.1%), depending on the breeding source. The generated and selected DH lines were tested in micro- and small-plot field experiments to identify promising lines for a pedigree breeding program. The improved AC method was integrated in a Hungarian temperate rice pedigree breeding program.

The growing global water shortage is an increasing challenge for the agricultural sector, which aims to produce sufficient quantity and quality of food and animal feed. In our study, effluent water from an intensive African catfish farm was irrigated on grain sorghum plants in four consecutive years. In our study the effects of the effluent on the N, P, K, Na content of the seeds, on the phenological parameters (plant height, relative chlorophyll content), the green mass, and on the grain yield of three varieties (‘Alföldi 1’, ‘Farmsugro 180’ and ‘GK Emese’) were investigated. Five treatments (Körös River (K) water and effluent (E) water: 30 and 45 mm weekly irrigation water dose; non-irrigated control) were applied with micro-spray irrigation. Compared to non-irrigated plants, effluent water did not negatively affect the N, P, K and Na contents of the grain crop. In terms of phenological parameters, the quality of the irrigation water (150–230 cm) had no negative effect on any of the measured parameters compared to the control (133–187 cm) values. In terms of biomass in 2020, grain yields were 89–109 g/plant with variety Alföldi 1, 64–91 g/plant with variety Farmsugro 180, and 86–110 g/plant with GK Emese.

Anther culture is an efficient biotechnological tool in modern plant breeding programs to produce new varieties and parental lines in hybrid seed productions. However, some bottlenecks—low induction rate, genotype dependency, albinism—restrict the widespread utilization of in vitro anther culture in rice breeding, especially in Oryza sativa ssp. indica (indica) genotypes, while an improved efficient protocol can shorten the process of breeding. Three different induction media (N₆NDK_, N₆NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested with five indica rice genotypes to increase the efficiency of in vitro androgenesis (number of calli and regenerated green plantlets). The production of calli was more efficient on the N₆NDK medium with an average 88.26 calli/100 anthers and N₆NDZ medium with an average of 103.88 calli/100 anthers as compared to Ali-1 with an average of 6.96 calli/100 anthers. The production of green plantlets was greater when calli was produced on N₆NDK medium (2.15 green plantlets/100 anthers) compared when produced on to N₆NDZ medium (1.18 green plantlets/100 anthers). Highest green plantlets production (4.7 green plantlets/100 anthers) was achieved when mMSNBK1 plant regeneration medium was used on calli produced utilizing N₆NDK induction medium. In the best overall treatment (N₆NDK induction medium and mMSNBK1 plant regeneration medium), four tested genotypes produced green plantlets. However, the genotype influenced the efficiency, and the green plantlets production ranged from 0.4 green plantlets/100 anthers to 8.4 green plantlets/100 anthers. The ploidy level of 106 acclimatized indica rice plantlets were characterized with flow cytometric analyses to calculate the percentage of spontaneous chromosome doubling. Altogether, 48 haploid-, 55 diploid-, 2 tetraploid- and 1 mixoploid plantlets were identified among the regenerant plantlets, and the spontaneous chromosome doubling percentage was 51.89%. Utilization of DH plants have been integrated as a routine method in the Hungarian rice breeding program. The tetraploid lines can be explored for their potential to offer new scopes for rice research and breeding directions in the future.

In this study, a special rice-energy willow/poplar agroforestry system was used to analyze the effects of reused water irrigation and mulching on soil salinity, earthworm biomass and abundance, soil organic matter (SOM) content and weed coverage in treerow-dependent habitats. After three-year-irrigation, we investigated the woody line (WL), the buffer zone (BZ) and the crop line habitats (CL). Between 2019 and 2021, we collected data on the distribution of soil-specific electrical conductivity (EC), ammonium-lactate soluble sodium (AL-Na) and exchangeable cations (Na⁺, K⁺, Ca²⁺, Mg²⁺) and soil NO₂⁻ + NO₃⁻-N contents from CL habitats irrigated with effluent water from an intensive catfish farm. Based on our results, significantly greater earthworm abundance (274 pc m⁻²) and earthworm biomass (54.0 g m⁻²) values were measured in WL than in BZ or CL habitats. There was no significant difference in weed coverage between the CL (0.61%) and BZ (1.91%), but weeds were significantly denser on the WL (12.3%). In the third year, the EC, AL-Na and ESP values were lower, but the SOM was higher in mulched soil (183.1 mS cm⁻¹, 253.1 mg kg⁻¹, 4.4 ESP% and 4.597%, respectively) than in un-mulched soil.

Nowadays, hyperspectral remote sensing data are widely used in nutrient management, crop yield forecasting and stress monitoring. These data can be acquired with satellites, drones and handheld spectrometers. In this research, handheld spectrometer data were validated by chlorophyll-a fluorescence measurements under natural cold stress. The performance of 16 rice cultivars with different origins and tolerances was monitored in the seedling stage. The studies were carried out under field conditions across two seasons to simulate different temperature regimes. Twenty-four spectral indices and eleven rapid chlorophyll-a fluorescence parameters were compared with albino plants. We identified which wavelengths are affected by low temperatures. Furthermore, the differences between genotypes were characterized by certain well-known and two newly developed (AAR and RAR) indices based on the spectral difference between the genotype and albino plant. The absorbance, reflectance and transmittance differences from the control are suitable for the discrimination of tolerant-sensitive varieties, especially based on their shape, peak and shifting distance. The following wavelengths are capable of determining the tolerant varieties, namely 548–553 nm, 667–670 nm, 687–688 nm and 800–950 nm in case of absorbance; above 700 nm for reflectance; and the whole spectrum (400–1100 nm) for transmittance.

Efficient utilization, treatment, and disposal of agricultural wastewater and sewage sludge are important environmental risks. In our research, effluent water from intensive aquaculture was evaluated for the irrigation of short rotation energy willow in a lysimeter experiment. Two different water types and their combinations were applied with weekly doses of 15, 30, and 60 mm, respectively. Our results revealed that implementing effluent water instead of fresh water could potentially increase the yield of the willow due to its higher nitrogen content (29 N mg/L). The biomass of irrigated short rotation coppice (SRC) willow plants were between 493–864 g/plant, 226–482 g/plant, and 268–553 g/plant dry weight during experiment period (2015–2017), respectively. However, due to the chemical properties (Na concentration, SAR value) of effluent water, the increase of the soil exchangeable sodium percentage (ESP) was significant and it can lead to soil degradation in the long term. The current study also investigated the relationship between chemical composition of the plant tissue and the irrigation water. In the case of K-levels of willow clones, an increasing trend was observed year-by-year. In terms of N and Na content was localized in leaf parts, especially in samples irrigated with effluent. Less N and Na values were detected in the stem and in the samples irrigated with surface water. In SRC willow plants, phosphorus was mostly localized in the stem, to a lower extent in the leaf part. The difference is mostly observed in the case of the amount of irrigation water, where the P content of the examined plant parts decreased with the increase of the amount of irrigation water. In the case of phenological observations, higher values of plant height were measured during diluted and effluent irrigation. Moreover, the SPAD of the plants irrigated with effluent water exceeded the irrigated ones with surface water.