Enzymes have played a crucial role in mankind’s challenges to use different types of biological systems for a diversity of applications. They are proteins that break down and convert complicated compounds to produce simple products. Fungal enzymes are compatible, efficient, and proper products for many uses in medicinal requests, industrial processing, bioremediation purposes, and agricultural applications. Fungal enzymes have appropriate stability to give manufactured products suitable shelf life, affordable cost, and approved demands. Fungal enzymes have been used from ancient times to today in many industries, including baking, brewing, cheese making, antibiotics production, and commodities manufacturing, such as linen and leather. Furthermore, they also are used in other fields such as paper production, detergent, the textile industry, and in drinks and food technology in products manufacturing ranging from tea and coffee to fruit juice and wine. Recently, fungi have been used for the production of more than 50% of the needed enzymes. Fungi can produce different types of enzymes extracellularly, which gives a great chance for producing in large amounts with low cost and easy viability in purified forms using simple purification methods. In the present review, a comprehensive trial has been advanced to elaborate on the different types and structures of fungal enzymes as well as the current status of the uses of fungal enzymes in various applications.

Bacterial cellulose (BC), a promising polysaccharide of microbial origin, is usually produced through synthetic (chemically defined) or natural media comprising of various environmental wastes (with exact composition unknown), through low-cost and readily available means. Various agricultural, industrial, and food processing wastes have been explored for sustainable BC production. Both conventional (using one variable at a time) and statistical approaches have been used for BC optimization, either during the static fermentation to obtain BC membranes (pellicle) or agitated fermentation that yields suspended fibers (pellets). Multiple studies have addressed BC production, however, the strategies applied in utilizing various wastes for BC production have not been fully covered. The present study reviews the nutritional requirements for maximal BC production including different optimization strategies for the cultivation conditions. Furthermore, commonly-used applications of BC, in various fields, including recent developments, and our current understanding have also been summarized.

The green synthesis of zinc oxide nanoparticles (ZnO-NPs) mediated fruit peel extract is gaining importance due to its cost-effectiveness and ecofriendly nature. Herein, ZnO-NPs were synthesized using pomegranate peel extract as a reducing and stabilizing agent. The synthesized ZnO-NPs were characterized using SEM, TEM-SAID, FT-IR, XRD, and particle size analysis. According to the findings, the ZnO-NPs were agglomerated into spherical and hexagonal shapes with an average diameter of 20 to 40 nm and crystallinity formed. The antimicrobial activity of ZnO-NPs against pathogenic microbes was significant in multiple applications, with 62.5 and 31.25 μg/ml of MIC for both Gram-positive and Gram-negative bacteria, respectively, and 125 and 250 μg/ml of MIC for Aspergillus niger and Aspergillus flavus, respectively. In addition, ZnO-NPs showed antioxidant activity with IC₅₀ = 240 and 250 μg/ml by DPPH and ABTS, respectively. All concentrations of ZnO-NPs significantly improved the germination of barley seed and shoot height, with the optimum concentration reaching 2 and 12 ppm of ZnO-NPs for both seed germination (90%) and shoot height (6.5), respectively, while the greatest root extension (6 cm) was observed at 2 ppm of ZnO-NPs. The mitotic index increased at lower nanoparticle concentrations and exposure times but declined considerably as the nanoparticle dose and exposure duration increased, until most concentrations reached 100% suppression after 12 h with various chromosomal abnormalities. The researchers were able to create efficient, eco-friendly, and simple multifunctional ZnO-NPs using a green synthetic strategy and, in the process, obtain a better understanding of the cytotoxicity and genotoxicity of ZnO-NPs in plant cells.

Bacterial cellulose (BC) is an ecofriendly biopolymer with diverse commercial applications. Its use is limited by the capacity of bacterial production strains and cost of the medium. Mining for novel organisms with well-optimized growth conditions will be important for the adoption of BC. In this study, a novel BC-producing strain was isolated from rotten fruit samples and identified as Lactiplantibacillus plantarum from 16S rRNA sequencing. Culture conditions were optimized for supporting maximal BC production using one variable at a time, Plackett–Burman design, and Box Behnken design approaches. Results indicated that a modified Yamanaka medium supported the highest BC yield (2.7 g/l), and that yeast extract, MgSO₄, and pH were the most significant variables influencing BC production. After optimizing the levels of these variables through Box Behnken design, BC yield was increased to 4.51 g/l. The drug delivery capacity of the produced BC membrane was evaluated through fabrication with sodium alginate and gentamycin antibiotic at four different concentrations. All membranes (normal and fabricated) were characterized by scanning electron microscope, Fourier transform-infrared spectroscopy, X-ray diffraction, and mechanical properties. The antimicrobial activity of prepared composites was evaluated by using six human pathogens and revealed potent antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus mutans, with no detected activity against Pseudomonas aeruginosa and Candida albicans.