Forest decline diseases are complex processes driven by biotic and abiotic factors. Although information about host–microbiome–environment interactions in agricultural systems is emerging rapidly, similar studies on tree health are still in their infancy. We used acute oak decline (AOD) as a model system to understand whether the rhizosphere physicochemical properties and microbiome are linked to tree health by studying these two factors in healthy and diseased trees located in three sites in different AOD stages—low, mid and severe. We found significant changes in the rhizosphere properties and microbiome composition across the different AOD sites and between the tree health conditions. Rhizosphere pH correlated with microbiome composition, with the microbial assemblages changing in more acidic soils. At the severe AOD site, the oak trees exhibited the lowest rhizosphere pH and distinct microbiome, regardless of their health condition, whereas, at the low and mid-stage AOD sites, only diseased trees showed lower pH and the microbial composition differed significantly from healthy trees. On these two sites, less extreme soil conditions and a high presence of host-beneficial microbiota were observed in the healthy oak trees. For the first time, this study gathers evidence of associations among tree health conditions, rhizosphere properties and microbiome as well as links aboveground tree decline symptoms to the belowground environment. This provides a baseline of rhizosphere community profiling of UK oak trees and paves the way for these associations to be investigated in other tree species suffering decline disease events.

Identifying patterns in roots spatial distribution and dynamics, and quantifying the root stocks, annual production and turnover rates at species level is essential for understanding plant ecological responses to local environmental factors and climate change. We studied selected root traits in four different stands, two European beech (Fagus sylvatica L.) forests and two Douglas fir (Pseudotsuga menziezii Mirb. Franco) plantations. Root system vertical distribution and dynamics were studied using sequential coring method and characterised into three root diameter size classes (0–2, 2–5 and 5–10 mm) sampled at three different soil depths (0–15, 15–30, 30–45 cm). Root annual production and turnover rates were analysed and quantified using Decision Matrix and Maximum-Minimum estimation approaches. The overall root mass (<10 mm diameter up to 0–45 cm soil depth) was higher in the beech forests than in the Douglas fir plantations. Some root traits, e.g., the overall root mass, the fine (0–2 mm) and small (2–5 mm) roots mass, differed significantly between the sampling plots rather than between the forest types. The root system revealed a tree species specific vertical distribution pattern. More than half of the fine and small roots biomass of the Douglas fir stands were allocated in the uppermost soil layer and decreased significantly with depths, while in the beech forests the biomass was more uniformly distributed and decreased gradually with increasing soil depth. Although both tree species belong to two different plant functional types and the stands were situated in two distantly located regions with different climatic and soil characteristics, we revealed similar trends in the root biomass and necromass dynamics, and close values for the annual production and turnover rates. The mean turnover rates for all studied stands obtained by sequential coring and Decision Matrix were 1.11 yr⁻¹ and 0.76 yr⁻¹ based on mean and maximum biomass data, respectively. They were similar to the averaged values suggested for Central and Northern European forests but higher compared to those reported from Southern Europe.

: Wood ash use, to raise pH and replace nutrients lost through forest harvesting, is well regulated in Scandinavia and Canada, but not yet in the UK. This experiment applied granulated wood ash from mixed tree thinnings to a lowland clay mineral soil at ~2.3 t ha−1 dose rate, after clearfell of western hemlock (Tsuga heterophylla (Raf.) Sarg.). With brash (~56 t ha−1 dry weight) as N resource, a 4 × 4 matrix of ash only, ash + brash, brash only and control treatments were planted with oak seedlings (Quercus robur L.). Soil survey was undertaken before ash application and followed over 10 years. By year 2, self-sown grass was dominant in the control and ash plots, and birch (Betula pendula, Roth.) was present across all treatments by year 4. In year 10, oak height was 25–29 per cent greater in brash and ash + brash plots, with DBH increased >20 per cent over the controls. Birch DBH was greater by 30 per cent in brash plots but was over 50 per cent in the ash + brash plots. Foliar concentrations of Ca, K and P significantly increased with ash addition in both oak and birch, as Al, Mn, Fe and Cr decreased. Soil pH (CaCl2) was reduced in all treatments in year 2 likely due to nitrification, during organic matter and needle breakdown, but recovered 8 years later to be significantly highest in ash and ash + brash plots. Mg, K, Ca, Na, Ba and S concentrations were higher in ash plot soils, but soil organic matter, total carbon and total nitrogen declined due to mineralization and uptake by grass and trees. Wood ash addition did not significantly increase heavy metal concentrations in either soil or foliage. In the long term, wood ash aided soil recovery and promoted tree growth in combination with the brash – it could be beneficial for tree growth after thinning/coppicing on heavy mineral soils, with the prerequisite of an existing ground cover. Aim: To quantify the effects of wood ash and brash addition over time to soils and tree growth after conifer clearfelling on a lowland clay soil site.

There is growing concern in the UK that available base cation pools in soil are declining due to the combined effects of acid deposition and forest harvesting. To help inform the issue, elemental mass balances for calcium (Ca), magnesium (Mg) and potassium (K) were calculated using more than 10-years (10–24 years) of data from the UK’s ICP Forest Intensive Monitoring Network (Level II) of plots, covering a range of soil types and three tree species—oak, Scots pine and Sitka spruce. Out of the ten sites investigated, small negative Ca balances were observed at three sites and negative K balances on two sites, all on acid geology and nutrient poor soils, which were previously heavily acidified due to acid deposition. There is sufficient Ca and K in the soil exchangeable pool to sustain forest growth on these sites, however, if the present rate of Ca and K loss continues forest health and productivity are likely to be threatened within a few forest rotations. Magnesium showed a positive balance at all but one site, partly sustained by marine deposition. Base cation budgets were significantly (p < 0.01) positively related to soil exchangeable cations and soil base saturation status. Six of the sites showed an increasingly statistically significant positive cation balance with time, attributed to a decline in leaching linked to recovery from acidification. This included the three sites with negative Ca balance, although Ca remained in deficit. One site (Alice Holt) exhibited a decreasing cation balance, driven by a continued significant decline in base cation deposition thought to be related to pollutant emission control. The results were used to simulate the impact of different forest biomass harvesting scenarios involving the removal of brown (extracted after needle drop) or green (extracted before needle drop) brash. Podzols and deep peats were found to be the most vulnerable to brash harvesting causing Ca and K imbalance, but problems also occurred on brown earths. Impacts were greatest for the extraction of green brash from higher productivity stands. Base cation balance calculations remain highly uncertain due to the restricted nature of available measurements and wide variation of some estimates, particularly inputs from mineral weathering. More data are required to check and improve model predictions to better guide forest harvesting practice and ensure sustainable forest management.

Atmospheric deposition is a critical driver of biogeochemical cycling in forest ecosystems. Among the pathways of deposition, throughfall (TF) — precipitation that has interacted with the forest canopy — plays a significant role in the input of atmospheric substances and canopy leachates to the forest floor. We investigated the influence of airborne pollen deposited in TF on its chemical composition across 60 Level II plots of the ICP Forests network in eight European countries. Based on 196 TF samples collected during the 2018 early vegetative season, we identified 53 pollen taxa, with Pinus , Picea , Fagus , and Quercus accounting for 91.4% of the total grains. Linear mixed-effects models revealed significant positive links between pollen deposition rates and TF fluxes of several ions, especially potassium (K⁺), which was explained by pollen abundance of all four main tree genera. Additionally, pollen of broadleaved taxa ( Fagus , Quercus ) influenced fluxes of the non-purgeable organic carbon (NPOC), dissolved organic nitrogen (DON), ammonium (NH ₄ ⁺ ), and phosphate (PO ₄ ³⁻ ). Conversely, broadleaved pollen showed a weak negative link with nitrate (NO₃⁻) fluxes, even if its interpretation requires a cautious approach, as several non-mutually exclusive pathways could explain it. Fractionation of the DOC pools highlighted a higher potential for microbial origin in broadleaves than in conifers. These findings provide the first continental-scale evidence of a link between pollen and TF water chemistry, concurrently analyzed on each water sample. This study underscores the ecological significance of pollen beyond plant reproduction and the need to consider flowering phenology and pollen release in forest deposition and nutrient assessments.

Ectomycorrhizae (EcM) are evolved mutualistic associations between soil fungi and plant roots. It has been shown that there can be species-specific differences in the ability to colonise roots. Colo­nised roots have increased longevity, greater resistance to pathogens, toxic elements in the soil and extreme con­ditions of temperature, acidity and moisture.EcM inocula are an essential resource in forest management. Nevertheless, measures being implemented worldwide to promote forest cover recovery to reduce atmospheric CO2 levels can cause ecological shifts. Shifts to ecological communities take place as species become more or less abundant, are wiped out or colonise new habitats. However, these changes may not be captured by metrics focusing on species richness alone.The lack of direct evidence of large-scale EcM temporal change in fungal community structure or function over time - commonly used in animal and plant research - is a basic, structural knowledge gap. Understanding temporal changes in community composition, whether via species losses and gains, or alterations to relative abundance and dominance is therefore essential to fathom the performance of terrestrial ecosystems, in particular the soil environment and its functions.This study will characterise EcM fungi in woodland sites of varying ages. This study uses a space-for-time approach, benefiting from a collection of sites part of a wider study on the biodiversity responses to woodland planting.