Identification of drought-tolerant, high-yielding rapeseed (Brassica napus L.) genotypes is critical for cultivation in semiarid regions, where water scarcity severely limits agricultural productivity. In this study, 38 winter rapeseed genotypes were evaluated using a randomized complete block design (RCBD) with three replications under two different environments (normal and drought stress). Drought tolerance was assessed based on multiple yield-related indices, while multivariate analyses, including PCA-biplot, GT-biplot, and cluster analysis (WARD method), were employed to dissect genotype performance. Additionally, fatty acid profiles were analysed to explore the impact of drought stress on seed oil composition. Significant differences among genotypes for seed yield were observed (P ≤ 0.05). Genotype G13 exhibited the highest yield (5.92 t ha⁻¹) under normal irrigation, while G10 achieved the highest yield (4.2 t ha⁻¹) under drought stress. Consistently, G10 also showed the highest values of STI (0.984), GMP (4.478), and YI (1.292), along with the lowest SSI (0.425) and YR (11.93%), confirming its drought tolerance. PCA-biplot analysis identified G33, G23, G18, G6, and G10 as the most stable and drought-tolerant genotypes. Cluster analysis supported these results, grouping the superior genotypes into the third cluster characterized by high STI, RDI, K2STI, and YSI values, and low TOL, SSI, and YR values. GT-biplot indicated strong correlations between YS and indices such as RDI, YSI, YI, and K2STI, while YP correlated with K1STI, TOL, SSI, and YR, distinguishing drought-susceptible high-yielding genotypes. Polygon view analysis highlighted G10 as the most superior genotype across drought indices. In terms of fatty acid composition, drought stress increased palmitic, linoleic, linolenic, erucic, and polyunsaturated fatty acids, while reducing stearic, oleic, saturated, and monounsaturated fatty acids. Notably, genotype G10 showed relatively smaller changes in its fatty acid composition under drought stress compared to other evaluated genotypes. This study identified several rapeseed genotypes with varying drought tolerance and yield performance, with G10 and G13 emerging as the most promising under drought and normal conditions, respectively. The identified genotypes offer promising genetic resources for breeding programs aiming to improve rapeseed adaptation to water-limited environments.