Coffee pulp, mucilage, and beans with mucilage were used to develop alcoholic beverages. The pulp of 45.3% pulp, 54.7% mucilage with seed, and 9.4% mucilage only were obtained during the wet processing of coffee. Musts were prepared for all to TSS (Total soluble solid) 18 °Bx and fermentation was carried out for 12–16 days until TSS decreased to 5 °Bx at 30 °C. Phenolic characteristics, chromatic structures, chemical parameters, and sensory characteristics were analyzed for the prepared alcoholic beverages. Methanol content, ester content, aldehyde, alcohol, total acidity, caffeine, polyphenols, flavonoids, chromatic structure, and hue of the alcoholic beverage from the pulp was 335 mg/L, 70.58 ppm, 9.15 ppm, 8.86 ABV%, 0.41%, 30.94 ppm, 845.7 mg GAE/g dry extract, 440.7 mg QE/g dry extract, 0.41, and 1.71, respectively. An alcoholic beverage from the pulp was found superior to an alcoholic beverage from mucilage with beans and a beverage from mucilage in sensory analysis. There is the possibility of developing fermented alcoholic beverages from coffee pulp and mucilage. However, further research is necessary for quality of the beans that were obtained from the fermentation with the mucilage.

Plant growth-promoting rhizobacteria (PGPR) are a group of root-associated bacteria that intimately interact with the plant roots and consequently enhance growth by extemporising nutrient retrieval or phytohormone production. We isolated and screened indigenous phosphate solubilising and auxinproducing PGPR from bamboo rhizosphere. 66 soil samples from bamboo (Bambusa nutans subsp. cupulata, B. balcooa and B. tulda) rhizospheres were collected from Dhanusha, Mahottari and Sarlahi districts, Nepal. 120 isolates of PGPR were obtained by serial dilution method in (PVK) agar and Luria Bertani agar. 92 out of 120 isolates of PGPR with the ability to solubilise phosphate were selected based on the halo colony ratio in PVK agar medium and auxin production in Luria Bertani agar. Among them, six isolates having high phosphate solubilising index and high production capacity of indole-3-acetic acid were further screened. Biochemical analysis revealed that these isolates belonged to the genus Pseudomonas. Phosphate solubilising index and indole-3- acetic acid production by six isolates ranged from 4.19±0.8 to 7.65±1.3, and IAA production ranged from 72.93±0.2 to 82.48±0.9µg/ml respectively. These isolates significantly increased shoot length (13.26±0.56cm), shoot fresh weight (16.26±1.02mg), shoot dry weight (10.56±0.09mg), root length (4.9±0.5cm), root fresh weight (7.56±1.05mg), root dry weight (3.21±0.01mg), and chlorophyll ‘a’ and chlrophyll‘b’ and carotenoid (2.16±0.01mg/g, 1.19±0.06mg/g and 0.92±0.01mg/g respectively) of B. nutans subsp. cupulata seedlings. This study suggests that PGPR isolated from bamboo rhizosphere demonstrated outstanding contribution to the growth promotion of seedlings of B. nutans subsp. cupulata as compared to negative control.

Extraction of Indole-3-acetic Acid from Plant Growth Promoting Rhizobacteria of Bamboo Rhizosphere and Its Effect on Biosynthesis of Chlorophyll in Bamboo Seedlings