In Thailand, pesticides containing organophosphates (OP) are frequently applied to crops to suppress insects. School children can be exposed to OPs on a daily basis, from food consumption to breathing and touching pesticides drifted near classrooms. Living in an agricultural area can also be one of the causes. As a result, it is important to monitor OPs residues in the food chain and biomarkers of exposure. The Gas Chromatography–Flame Photometric Detector method was employed to examine the relationship between OPs residue and DAPs (Diakly phosphate) in four targeted locations in Thailand, as well as to examine the residues of OPs in vegetable samples and DAPs in 395 school children’s urine samples. Vegetables were found to contain at least one OP, with chlorpyrifos being the most prevalent. The OPs detected frequencies for Sakon Nakhon, Chiang Mai, Phang Nga, and Pathum Thani are 96.1%, 94%, 91.7%, and 83.3%, respectively. The overall centration level of OPs showed 0.3261 mg/kg, 0.0636 mg/kg, 0.0023 mg/kg, 0.0150 mg/kg, 0.2003 mg/kg, 0.0295 mg/kg, and 0.0034 mg/kg for diazinon, dimethoate, pirimiphos-methyl, chlorpyrifos, profenofos, ethion, and triazophos, respectively. Nearly 98% of school children were detected with at least one DAP. The overall level of dimethyl phosphate metabolites (5.258 µmole/mole creatinine) in urine samples is higher than diethyl phosphate metabolites (2.884 µmole/mole creatinine), especially in the case of Pathum Thani. Our findings show a consistent relationship between OPs in vegetables from wet markets and DAPs in urine samples of school children in various parts of Thailand.

This study examined the effectiveness of Thailand’s Q-GAP initiative in raising cabbage farmers’ awareness regarding food safety assurance and improving their pesticide use practices by comparing 41 Q-GAP-certified and 90 uncertified farmers in Chiang Mai Province, Thailand. The research methods included a questionnaire survey administered to the participating farmers and laboratory pesticide residue testing of their cabbage produce samples. The research found that certified farmers had a significantly more heedful attitude than uncertified farmers toward the effects of pesticide use on the users, consumers, and the environment. This is supported by the higher level of Q-GAP training experiences. Compared to uncertified farmers, a significantly lower proportion of certified farmers also applied either insecticide, fungicide, or herbicide, with significantly less frequencies when including in the analysis those who did not use the particular pesticide. In the pesticide residue analysis, no significant difference was found between the two types of farms regarding the number of farms detected with maximum residue level exceedance in the sample produce and regarding the results of the aggregate organophosphate pesticide analysis. However, it was found that the produce of the uncertified farmers contained significantly more residue in the aggregate synthetic pyrethroid pesticide analysis as compared to that of the certified farmers. These findings indicate the Q-GAP-certified farmers’ progress in process control from earlier Q-GAP studies on compliance. However, considering the limited achievements of certified farmers in pesticide handling and recordkeeping, and those of the Department of Agriculture officers in auditing, there is a need for more governmental efforts, especially in these areas.

Agriculture is vital for economic growth and livelihoods in northern Thailand, yet it is challenged by hazardous practices such as improper chemical use and machinery accidents. This study aimed to evaluate the risk and determine the relationship between pesticide exposure and knowledge, attitudes, and safety behaviors. This cross-sectional study was conducted between October and December 2017 in the San Pa Tong district of Chiang Mai, Thailand. Data were collected through structured questionnaires and face-to-face interviews with 280 farmers. The findings indicated that knowledge and safety behavior scores varied significantly with educational attainment and training received. Farmers with higher education levels or those who had undergone training exhibited better knowledge and safer behavioral practices, whereas income levels did not significantly affect knowledge or safety behaviors. The pesticide exposure risk level was associated with the study variables, with those recognizing a ‘normal’ risk level demonstrating greater knowledge and safer behaviors than those who reported the risk level as safe or at risk. A strong positive correlation between knowledge and safety behavior was observed, indicating that increased awareness can result in more cautious practices. These findings suggest that educational and training programs are crucial for promoting safer pesticide use among farmers. Although attitudes toward pesticide safety varied based on income, education, and training positively influenced knowledge and behavior. The study concludes that understanding farmers’ perceptions of pesticide risks is essential for designing and implementing effective educational interventions.

This study investigated the effectiveness of Thailand’s public good agricultural practices certification initiative (Q-GAP) in improving export-oriented mango farmers’ awareness and practices relating to food safety and quality assurance. Two groups of certified farmers in Northern Thailand (one from Chiang Mai Province and the other from Phitsanulok Province), comprising a total of 96 samples, were surveyed for comparative analysis. Many items of the comparison showed no significant differences between the two groups of farmers (specifically, the use of synthetic pesticides and non-synthetic pest control methods, record-keeping, and agrochemical safety and handling practices). One significant difference worth mentioning was related to the farmers’ ability to link the goal of the Q-GAP policy to food safety assurance; when compared to Phitsanulok, approximately 35% more farmers in Chiang Mai demonstrated such an understanding. This contradicted the more experience and days of training on Q-GAP the farmers in Phitsanulok had in comparison to those in Chiang Mai. Moreover, the rates of understanding in both areas were significantly lower than those identified in previous Q-GAP studies of less export-oriented crops. The export-oriented mango farmers might be trained to regard Q-GAP certification as a type of license to export their mango produce, rather than as a tool to improve their food safety and quality assurance.

Cholinesterase (ChE) is an essential enzyme for nervous system function, and its activity in the blood serves as a biomarker for pesticide exposure, necessitating accessible assessment methods in resource-limited areas. This study focused on developing and validating a micro-electrometric method to rapidly and reliably measure ChE activity in human blood to monitor pesticide exposure. A micro-electrometric method was designed to evaluate ChE activity in whole-blood samples, involving incubation with organophosphate and carbamate pesticides and subsequent pH measurement, and its performance was compared to that of the Ellman method. The optimized method was strongly correlated with the Ellman method (R² = 0.9147), effectively measuring dose-dependent ChE inhibition by mevinphos and carbofuran with stabilization after 10 min, demonstrating a high sensitivity suitable for field applications. The micro-electrometric method is easy, accurate, and inexpensive for measuring ChE activity. It has been proven to work by comparing it to other methods; therefore, it can be used in clinical and research settings with limited resources.

In low- and middle-income countries, notably in rural agricultural populations exposed to environmental and occupational dangers, respiratory impairment and noncommunicable diseases (NCDs) are major public health issues. This cross-sectional study examined the associations between lung function, functional capacity, and cardiovascular responses to the Six-Minute Walk Test (6MWT) in 137 adults from San Pa Tong District, Northern Thailand. Lung function was assessed using spirometry, and participants were classified accordingly. Hemodynamic parameters, including blood pressure, heart rate, rate-pressure product, and oxygen saturation, were measured before and after the 6MWT. Participants with impaired lung function walked significantly shorter distances (p = 0.004), and walking distance was positively correlated with forced vital capacity (FVC) and forced expiratory volume in one second (FEV₁). Logistic regression confirmed that walking distance independently predicted lung function impairment after adjusting for age and sex. Cardiovascular responses to exercise also varied significantly across demographic subgroups. These findings support the use of the 6MWT as a practical, cost-effective, and scalable method for detecting lung function impairments in resource-limited rural settings. To our knowledge, this is among the first studies to demonstrate the predictive value of the 6MWT for lung function impairment in a Southeast Asian agricultural population.