This page has only limited features, please log in for full access.

Unclaimed
Waldiceu A. Verri
Department of Pathology, Center of Biological Sciences, Paraná State University of Londrina, Londrina, Brazil

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Original papers
Published: 23 July 2021 in Photochemical & Photobiological Sciences
Reads 0
Downloads 0

Cordia verbenacea DC (Boraginaceae) is a flowering shrub found along the Brazilian Atlantic Forest, Brazilian coast, and low areas of the Amazon. The crude extract of its leaves is widely used in Brazilian folk medicine as an anti-inflammatory, both topically and orally. The aim of this study is to evaluate the activity of C. verbenacea ethanolic leaves extract (CVE) against UVB-triggered cutaneous inflammation and oxidative damage in hairless mice. CVE treatment recovered cutaneous antioxidant capacity demonstrated by scavenging ABTS+ free radical and iron-reducing antioxidant potential evaluated by FRAP. CVE also controlled the following UV-triggered events in the skin: reduced glutathione (GSH) depletion, catalase activity decrease, and superoxide anion (O⋅–) build-up. Furthermore, mice treated with CVE exhibited less inflammation, shown by the reduction in COX-2 expression, TNF-α, IL-1β, IL-6, edema, and neutrophil infiltration. CVE also regulated epidermal thickening and sunburn cells, reduced dermal mast cells, and preserved collagen integrity. The best results were obtained using 5% CVE-added emulsion. The present data demonstrate that topical administration of CVE presents photochemoprotective activity in a mouse model of UVB inflammation and oxidative stress. Because of the intricate network linking inflammation, oxidative stress, and skin cancer, these results also indicate the importance of further studies elucidating a possible role of C. verbenacea in the prevention of UVB-induced skin cancer and evaluating a potential synergy between CVE and sunscreens in topical products against UVB damaging effects to the skin.

ACS Style

Cristina P. B. Melo; Priscila Saito; David L. Vale; Camilla C. A. Rodrigues; Ingrid C. Pinto; Renata M. Martinez; Julia R. Bezerra; Marcela M. Baracat; Waldiceu A. Verri; Yris Maria Fonseca-Bazzo; Sandra R. Georgetti; Rubia Casagrande. Protection against UVB deleterious skin effects in a mouse model: effect of a topical emulsion containing Cordia verbenacea extract. Photochemical & Photobiological Sciences 2021, 1 -19.

AMA Style

Cristina P. B. Melo, Priscila Saito, David L. Vale, Camilla C. A. Rodrigues, Ingrid C. Pinto, Renata M. Martinez, Julia R. Bezerra, Marcela M. Baracat, Waldiceu A. Verri, Yris Maria Fonseca-Bazzo, Sandra R. Georgetti, Rubia Casagrande. Protection against UVB deleterious skin effects in a mouse model: effect of a topical emulsion containing Cordia verbenacea extract. Photochemical & Photobiological Sciences. 2021; ():1-19.

Chicago/Turabian Style

Cristina P. B. Melo; Priscila Saito; David L. Vale; Camilla C. A. Rodrigues; Ingrid C. Pinto; Renata M. Martinez; Julia R. Bezerra; Marcela M. Baracat; Waldiceu A. Verri; Yris Maria Fonseca-Bazzo; Sandra R. Georgetti; Rubia Casagrande. 2021. "Protection against UVB deleterious skin effects in a mouse model: effect of a topical emulsion containing Cordia verbenacea extract." Photochemical & Photobiological Sciences , no. : 1-19.

Journal article
Published: 06 July 2021 in Journal of Psychiatric Research
Reads 0
Downloads 0

Brain-derived neurotrophic factor (BDNF) and the immune-inflammatory response system (IRS) have been implicated in the pathophysiology of schizophrenia. However, no research examined the associations between BDNF and immune activation both before and after treatment in antipsychotic-naïve first episode psychosis (AN-FEP). This study aims to examine serum BDNF levels and their association with IRS and the compensatory immune-regulatory reflex system (CIRS) in AN-FEP before and after risperidone treatment. We included 31 AN-FEP and 22 healthy controls. AN-FEP showed reduced levels of BDNF as compared to controls, and BDNF levels normalized after treatment with risperidone. BDNF levels were inversely correlated with a greater IRS response. Higher levels of IRS/CIRS biomarkers were associated with lower levels of BDNF including M1 macrophage, T-helper (Th)-1, Th-2, and Th-17, and T-regulatory (Treg) cell responses. Our findings indicate that AN-FEP is characterized by decreased levels of BDNF, which are normalized after treatment with risperidone. BDNF levels were inversely associated with activated immune-inflammatory pathways. The findings support the hypothesis that, increased IRS is linked to neurotoxicity, and that a decrease in BDNF may be part of the IRS/CIRS responses in FEP and, thus, be involved in the development of psychosis.

ACS Style

Mariane Nunes Noto; Michael Maes; Sandra Odebrecht Vargas Nunes; Vanessa Kiyomi Ota; Daniel Cavalcante; Giovany Oliveira; Ana C. Rossaneis; Waldiceu A. Verri; Quirino Cordeiro; Sintia Iole Belangero; Ary Gadelha; Cristiano Noto; Rodrigo Affonseca Bressan. BDNF in antipsychotic naive first episode psychosis: Effects of risperidone and the immune-inflammatory response system. Journal of Psychiatric Research 2021, 141, 206 -213.

AMA Style

Mariane Nunes Noto, Michael Maes, Sandra Odebrecht Vargas Nunes, Vanessa Kiyomi Ota, Daniel Cavalcante, Giovany Oliveira, Ana C. Rossaneis, Waldiceu A. Verri, Quirino Cordeiro, Sintia Iole Belangero, Ary Gadelha, Cristiano Noto, Rodrigo Affonseca Bressan. BDNF in antipsychotic naive first episode psychosis: Effects of risperidone and the immune-inflammatory response system. Journal of Psychiatric Research. 2021; 141 ():206-213.

Chicago/Turabian Style

Mariane Nunes Noto; Michael Maes; Sandra Odebrecht Vargas Nunes; Vanessa Kiyomi Ota; Daniel Cavalcante; Giovany Oliveira; Ana C. Rossaneis; Waldiceu A. Verri; Quirino Cordeiro; Sintia Iole Belangero; Ary Gadelha; Cristiano Noto; Rodrigo Affonseca Bressan. 2021. "BDNF in antipsychotic naive first episode psychosis: Effects of risperidone and the immune-inflammatory response system." Journal of Psychiatric Research 141, no. : 206-213.

Original investigation
Published: 18 June 2021 in The World Journal of Biological Psychiatry
Reads 0
Downloads 0

This study examined cognition-immune interactions, specifically executive function, working memory, peripheral levels of tumour necrosis factor-alpha (TNF-α), and soluble tumour necrosis factor receptors-1 and -2 (sTNFR1 and 2) levels in patients in comparison with controls. Thirty-one BD participants and twenty-seven controls participated in the study. The neurocognitive assessment was performed through three of CogState Research BatteryTM tasks for executive function and working memory. Plasma levels of TNF-α, sTNFR1, and sTNFR2 were measured after overnight fasting. Sociodemographic data and symptom severity of depression and mania were assessed. BD presented a significantly worse performance in the working memory task (p = 0.005) and higher levels of TNF-α (p = 0.043) in comparison to controls. A trend level of significance was found for sTNFR1 between groups (p = 0.082). Among BD participants, there were significant correlations between sTNFR2 and neurocognitive tasks (Groton Maze Learning Task: ρ = 0.54, p = 0.002; Set-Shifting Task: ρ = 0.37, p = 0.042; and the Two-Back Task: ρ=-0.49, p = 0.005), and between sTNFR1 and mania, depression and anxiety symptoms (respectively ρ = 0.37, p = 0.038; ρ=-0.38, p = 0.037; and ρ = 0.42, p = 0.002). TNF-α and its receptors might be an important variable in cognitive impairment in BD. Future studies might focus on the development of anti-inflammatory therapeutic targets for cognitive dysfunction in BD.

ACS Style

Robson Zazula; Seetal Dodd; Olivia M. Dean; Michael Berk; Chiara C. Bortolasci; Waldiceu A. Verri; Heber O. Vargas; Sandra O. V Nunes. Cognition-immune interactions between executive function and working memory, tumour necrosis factor-alpha (TNF-alpha) and soluble TNF receptors (sTNFR1 and sTNFR2) in bipolar disorder. The World Journal of Biological Psychiatry 2021, 1 -11.

AMA Style

Robson Zazula, Seetal Dodd, Olivia M. Dean, Michael Berk, Chiara C. Bortolasci, Waldiceu A. Verri, Heber O. Vargas, Sandra O. V Nunes. Cognition-immune interactions between executive function and working memory, tumour necrosis factor-alpha (TNF-alpha) and soluble TNF receptors (sTNFR1 and sTNFR2) in bipolar disorder. The World Journal of Biological Psychiatry. 2021; ():1-11.

Chicago/Turabian Style

Robson Zazula; Seetal Dodd; Olivia M. Dean; Michael Berk; Chiara C. Bortolasci; Waldiceu A. Verri; Heber O. Vargas; Sandra O. V Nunes. 2021. "Cognition-immune interactions between executive function and working memory, tumour necrosis factor-alpha (TNF-alpha) and soluble TNF receptors (sTNFR1 and sTNFR2) in bipolar disorder." The World Journal of Biological Psychiatry , no. : 1-11.

Journal article
Published: 18 June 2021 in Toxicon
Reads 0
Downloads 0

Scorpionism is a public health burden in Brazil. Tityus bahiensis is responsible for most accidents in the Southeastern region of Brazil. Here, the hyperalgesic mechanisms of Tityus bahiensis venom were investigated, focusing on the role of pro-inflammatory cytokines (tumor necrosis factor alpha [TNF-α] and interleukin 1 beta [IL-1β]) and activation of the transcription factor NFκB. Intraplantar (i.pl.) administration of Tityus bahiensis venom (0.2, 0.6, 1.2 and 2.4 μg/20 μL i.pl.) induced mechanical hyperalgesia and thermal hyperalgesia. The 2.4 μg dose of Tityus bahiensis venom induced overt pain-like behavior and increased myeloperoxidase (MPO) and N-acetyl-beta-D-glucosaminidase (NAG) activities, TNF-α and IL-1β levels in the paw tissue. Systemic pre-treatment with etanercept (soluble TNF-α receptor; 10 mg/kg), IL-1ra (IL-1 receptor antagonist; 30 mg/kg) and pyrrolidine dithiocarbamate (PDTC, nuclear factor kappa B [NFκB] inhibitor; 100 mg/kg) inhibited Tityus bahiensis venom-induced mechanical and thermal hyperalgesia, MPO and NAG activity and overt pain-like behavior. These data demonstrate the involvement of TNF-α and IL-1β signaling as well as NFκB activation in Tityus bahiensis venom-induced mechanical and thermal hyperalgesia, overt pain-like behavior, and MPO activity and NAG activity, indicating thus, that targeting these mechanisms might contribute to reducing the pain in this scorpionism.

ACS Style

Camila R. Ferraz; Marília F. Manchope; Ketlem C. Andrade; Telma Saraiva-Santos; Anelise Franciosi; Tiago H. Zaninelli; Julia Bagatim-Souza; Sergio M. Borghi; Denise M. Cândido; Irene Knysak; Rubia Casagrande; Fábio H. Kwasniewski; Waldiceu A. Verri. Peripheral mechanisms involved in Tityus bahiensis venom-induced pain. Toxicon 2021, 200, 3 -12.

AMA Style

Camila R. Ferraz, Marília F. Manchope, Ketlem C. Andrade, Telma Saraiva-Santos, Anelise Franciosi, Tiago H. Zaninelli, Julia Bagatim-Souza, Sergio M. Borghi, Denise M. Cândido, Irene Knysak, Rubia Casagrande, Fábio H. Kwasniewski, Waldiceu A. Verri. Peripheral mechanisms involved in Tityus bahiensis venom-induced pain. Toxicon. 2021; 200 ():3-12.

Chicago/Turabian Style

Camila R. Ferraz; Marília F. Manchope; Ketlem C. Andrade; Telma Saraiva-Santos; Anelise Franciosi; Tiago H. Zaninelli; Julia Bagatim-Souza; Sergio M. Borghi; Denise M. Cândido; Irene Knysak; Rubia Casagrande; Fábio H. Kwasniewski; Waldiceu A. Verri. 2021. "Peripheral mechanisms involved in Tityus bahiensis venom-induced pain." Toxicon 200, no. : 3-12.

Article
Published: 15 March 2021 in Journal of Drug Targeting
Reads 0
Downloads 0

Ultraviolet B (UVB) irradiation causes free radical production, increase inflammation and oxidative stress, thus, supporting the use of antioxidants by topical administration as therapeutic approaches. Quercetin (QC) is a flavonoid with antioxidant activity, however, high liposolubility makes it difficult to remain in the viable skin layer. Thus, this study evaluated whether microencapsulation of QC would enhance its activity in comparison with the same dose of free QC (non-active dose) and unloaded-microcapsules added in formulation for topical administration in a mouse model of UVB irradiation targeting the skin. Topical formulation containing Quercetin-loaded microcapsules (TFcQCMC) presents physico-chemical (colour, consistence, phase separation and pH) and functional antioxidant stability at 4 °C, room temperature and 40 °C for 6 months. TFcQCMC inhibited the UVB-triggered depletion of antioxidants observed by GSH (reduced glutathione), ability to reduce iron, ability to scavenge 2,2’-azinobis radical and catalase activity. TFcQCMC also inhibited markers of oxidation (lipid hydroperoxides and superoxide anion production). Concerning inflammation, TFcQCMC reduced the production of inflammatory cytokines, matrix metalloproteinase-9 activity, skin edoema, collagen fibre damage, myeloperoxidase activity/neutrophil recruitment, mast cell and sunburn cell counts. The pharmacological activity of TFcQCMC was not shared by the same pharmaceutical form containing the same dose of free QC or unloaded control microcapsules.

ACS Style

David L. Vale; Renata M. Martinez; Daniela C. Medeiros; Camila da Rocha; Natália Sfeir; Renata F. V. Lopez; Fabiana T. M. C. Vicentini; Waldiceu A. Verri Jr; Sandra R. Georgetti; Marcela M. Baracat; Rúbia Casagrande. A topical formulation containing quercetin-loaded microcapsules protects against oxidative and inflammatory skin alterations triggered by UVB irradiation: enhancement of activity by microencapsulation. Journal of Drug Targeting 2021, 1 -15.

AMA Style

David L. Vale, Renata M. Martinez, Daniela C. Medeiros, Camila da Rocha, Natália Sfeir, Renata F. V. Lopez, Fabiana T. M. C. Vicentini, Waldiceu A. Verri Jr, Sandra R. Georgetti, Marcela M. Baracat, Rúbia Casagrande. A topical formulation containing quercetin-loaded microcapsules protects against oxidative and inflammatory skin alterations triggered by UVB irradiation: enhancement of activity by microencapsulation. Journal of Drug Targeting. 2021; ():1-15.

Chicago/Turabian Style

David L. Vale; Renata M. Martinez; Daniela C. Medeiros; Camila da Rocha; Natália Sfeir; Renata F. V. Lopez; Fabiana T. M. C. Vicentini; Waldiceu A. Verri Jr; Sandra R. Georgetti; Marcela M. Baracat; Rúbia Casagrande. 2021. "A topical formulation containing quercetin-loaded microcapsules protects against oxidative and inflammatory skin alterations triggered by UVB irradiation: enhancement of activity by microencapsulation." Journal of Drug Targeting , no. : 1-15.

Journal article
Published: 01 March 2021 in International Journal of Biological Macromolecules
Reads 0
Downloads 0

Jararhagin is a hyperalgesic metalloproteinase from Bothrops jararaca venom. In rodents, jararhagin induces nociceptive behaviors that correlate with an increase in peripheral cytokine levels. However, the role of the spinal cord glia in pain processing after peripheral stimulus of jararhagin has not been investigated. Aiming to explore this proposal, mice received intraplantar (i.pl.) injection of jararhagin and the following parameters were evaluated: hyperalgesia, spinal cord TNF-α, IL-1β levels, and CX3CR1, GFAP and p-NFκB activation. The effects of intrathecal (i.t.) injection of TNF-α soluble receptor (etanercept), IL-1 receptor antagonist (IL-1Ra), and inhibitors of NFκB (PDTC), microglia (minocycline) and astrocytes (α-aminoadipate) were investigated. Jararhagin inoculation induced cytokine production (TNF-α and IL-1β) in the spinal cord, which was reduced by treatment with PDTC (40% and 50%, respectively). Jararhagin mechanical hyperalgesia and cytokine production were inhibited by treatment with etanercept (67%), IL-1Ra (60%), PDTC (70%), minocycline (60%) and α-aminoadipate (45%). Furthermore, jararhagin induced an increase in p-NFκB, CX3CR1 and GFAP detection in the spinal cord indicating activation of NFκB, microglia and astrocytes. These results demonstrate for the first time that jararhagin-induced mechanical hyperalgesia is dependent on spinal cord activation of glial cells, consequent NFκB activation, and cytokine production in mice.

ACS Style

Camila R. Ferraz; Thacyana T. Carvalho; Victor Fattori; Telma Saraiva-Santos; Felipe A. Pinho-Ribeiro; Sergio M. Borghi; Marília F. Manchope; Tiago H. Zaninelli; Thiago M. Cunha; Rubia Casagrande; Patricia B. Clissa; Waldiceu A. Verri. Jararhagin, a snake venom metalloproteinase, induces mechanical hyperalgesia in mice with the neuroinflammatory contribution of spinal cord microglia and astrocytes. International Journal of Biological Macromolecules 2021, 179, 610 -619.

AMA Style

Camila R. Ferraz, Thacyana T. Carvalho, Victor Fattori, Telma Saraiva-Santos, Felipe A. Pinho-Ribeiro, Sergio M. Borghi, Marília F. Manchope, Tiago H. Zaninelli, Thiago M. Cunha, Rubia Casagrande, Patricia B. Clissa, Waldiceu A. Verri. Jararhagin, a snake venom metalloproteinase, induces mechanical hyperalgesia in mice with the neuroinflammatory contribution of spinal cord microglia and astrocytes. International Journal of Biological Macromolecules. 2021; 179 ():610-619.

Chicago/Turabian Style

Camila R. Ferraz; Thacyana T. Carvalho; Victor Fattori; Telma Saraiva-Santos; Felipe A. Pinho-Ribeiro; Sergio M. Borghi; Marília F. Manchope; Tiago H. Zaninelli; Thiago M. Cunha; Rubia Casagrande; Patricia B. Clissa; Waldiceu A. Verri. 2021. "Jararhagin, a snake venom metalloproteinase, induces mechanical hyperalgesia in mice with the neuroinflammatory contribution of spinal cord microglia and astrocytes." International Journal of Biological Macromolecules 179, no. : 610-619.

Journal article
Published: 27 February 2021 in Journal of Ethnopharmacology
Reads 0
Downloads 0

Sphagneticola trilobata (L.) Pruski is a plant species belonging to the Asteraceae family. Kaurenoid acid (KA) is a diterpene metabolite and one of the active ingredients of Sphagneticola trilobata (L.) Pruski. Extracts containing KA are used in traditional medicine to treat pain, inflammation, and infection. The goal of the present study was to investigate the in vivo effects of KA (1–10 mg/kg, per oral gavage) upon LPS inoculation in mice by intraperitoneal (i.p.) or intraplantar (i.pl.; subcutaneous plantar injection) routes at the dose of 200 ng (200 μL or 25 μL, respectively). In LPS paw inflammation, mechanical and thermal hyperalgesia MPO activity and oxidative imbalance (TBARS, GSH, ABTS and FRAP assays) were evaluated. In LPS peritonitis we evaluated leukocyte migration, cytokine production, oxidative stress, and NF-κB activation. KA inhibited LPS-induced mechanical and thermal hyperalgesia, MPO activity and modulated redox status in the mice paw. Pre- and post-treatment with KA inhibited migration of neutrophils and monocytes in LPS peritonitis. KA inhibited the pro-inflammatory/hyperalgesic cytokine (e.g., TNF-α, IL-1β and IL-33) production while enhanced anti-inflammatory/analgesic cytokine IL-10 in peritoneal cavity. In agreement with the effect of KA over pro-inflammatory cytokines it inhibited oxidative stress (total ROS, superoxide production and superoxide positive cells) and NF-κB activation during peritonitis. KA efficiently dampens LPS-induced peritonitis and hyperalgesia in vivo, suggesting it as a suitable candidate to control excessive inflammation and pain during gram-negative bacterial infections and bringing mechanistic explanation to the ethnopharmacological application of Sphagneticola trilobata (L.) Pruski in inflammation and infection.

ACS Style

Sergio M. Borghi; Sandra S. Mizokami; Thacyana T. Carvalho; Fernanda S. Rasquel-Oliveira; Camila R. Ferraz; Victor Fattori; Thiago H. Hayashida; Jean P.S. Peron; Doumit Camilios-Neto; Sergio R. Ambrosio; Nilton S. Arakawa; Rubia Casagrande; Waldiceu A. Verri. The diterpene from Sphagneticola trilobata (L.) Pruski, kaurenoic acid, reduces lipopolysaccharide-induced peritonitis and pain in mice. Journal of Ethnopharmacology 2021, 273, 113980 .

AMA Style

Sergio M. Borghi, Sandra S. Mizokami, Thacyana T. Carvalho, Fernanda S. Rasquel-Oliveira, Camila R. Ferraz, Victor Fattori, Thiago H. Hayashida, Jean P.S. Peron, Doumit Camilios-Neto, Sergio R. Ambrosio, Nilton S. Arakawa, Rubia Casagrande, Waldiceu A. Verri. The diterpene from Sphagneticola trilobata (L.) Pruski, kaurenoic acid, reduces lipopolysaccharide-induced peritonitis and pain in mice. Journal of Ethnopharmacology. 2021; 273 ():113980.

Chicago/Turabian Style

Sergio M. Borghi; Sandra S. Mizokami; Thacyana T. Carvalho; Fernanda S. Rasquel-Oliveira; Camila R. Ferraz; Victor Fattori; Thiago H. Hayashida; Jean P.S. Peron; Doumit Camilios-Neto; Sergio R. Ambrosio; Nilton S. Arakawa; Rubia Casagrande; Waldiceu A. Verri. 2021. "The diterpene from Sphagneticola trilobata (L.) Pruski, kaurenoic acid, reduces lipopolysaccharide-induced peritonitis and pain in mice." Journal of Ethnopharmacology 273, no. : 113980.

Journal article
Published: 16 February 2021 in Theriogenology
Reads 0
Downloads 0

Mycotoxins are natural contaminants of food and feed occurring worldwide. Deoxynivalenol (DON) and fumonisin B1 (FB1) are the most frequent fusariotoxins and induce immune and intestinal toxicity in humans and animals. Recently, an association between mycotoxins exposure and impaired fertility has been suggested. However, the effects of these mycotoxins on the reproductive system are not well established. This study aimed to evaluate the effects of FB1 and DON, in combination or alone, on the ovarian morphology and oxidative responses using porcine explants. Seventy-two explants were obtained from six pigs and submitted to the following treatments: control (MEM medium), DON (10 μM), FB1 (100 μM FB1), and DON + FB1 (10 μM + 100 μM). Histological and immunohistochemical assays were performed to evaluate ovarian changes, cell proliferation, and apoptosis. Oxidative stress response was evaluated through lipid peroxidation and antioxidant capacity response assays. The exposure to mycotoxins induced significant histological changes in the ovaries, which were characterized by a decrease in viable follicles and increase in degenerated follicles. A significant decrease in granulosa cell proliferation was observed in explants exposed to all mycotoxins. In addition the multi-contaminated treatment was responsible for an increase in the cell apoptosis index of growing follicles. On the other hand, the FB1 and multi-contaminated treatments induced a significant decrease in lipid peroxidation accompanied by an increase in antioxidant responses. Altogether, our results indicate a reproductive toxicity induced by fusariotoxins. Moreover, mycotoxins, alone or in combination, modulate oxidative stress response, interfering with the production of free radicals and affecting the reproductive capacity of pigs.

ACS Style

Juliana Rubira Gerez; Thaynara Camacho; Victor Hugo Brunaldi Marutani; Ricardo Luís Nascimento de Matos; Miriam Sayuri Hohmann; Waldiceu Aparecido Verri Júnior; Ana Paula F.R. L. Bracarense. Ovarian toxicity by fusariotoxins in pigs: Does it imply in oxidative stress? Theriogenology 2021, 165, 84 -91.

AMA Style

Juliana Rubira Gerez, Thaynara Camacho, Victor Hugo Brunaldi Marutani, Ricardo Luís Nascimento de Matos, Miriam Sayuri Hohmann, Waldiceu Aparecido Verri Júnior, Ana Paula F.R. L. Bracarense. Ovarian toxicity by fusariotoxins in pigs: Does it imply in oxidative stress? Theriogenology. 2021; 165 ():84-91.

Chicago/Turabian Style

Juliana Rubira Gerez; Thaynara Camacho; Victor Hugo Brunaldi Marutani; Ricardo Luís Nascimento de Matos; Miriam Sayuri Hohmann; Waldiceu Aparecido Verri Júnior; Ana Paula F.R. L. Bracarense. 2021. "Ovarian toxicity by fusariotoxins in pigs: Does it imply in oxidative stress?" Theriogenology 165, no. : 84-91.

Journal article
Published: 04 February 2021 in Journal of Photochemistry and Photobiology B: Biology
Reads 0
Downloads 0

Photochemoprotection of the skin can be achieved by inhibiting inflammation and oxidative stress, which we tested using Cordia verbenacea extract, a medicinal plant known for its rich content of antioxidant molecules and anti-inflammatory activity. In vitro antioxidant evaluation of Cordia verbenacea leaves ethanolic extract (CVE) presented the following results: ferric reducing antioxidant power (886.32 μM equivalent of Trolox/g extract); IC50 of 19.128 μg/ml for scavenging 2,2-diphenyl-1-picrylhydrazyl; IC50 of 12.48 μg/mL for scavenging 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid); decrease of hydroperoxides from linoleic acid (IC50 of 10.20 μg/mL); inhibition of thiobarbituric acid reactive substances (IC50 8.90 μg/mL); iron-chelating ability in bathophenanthroline iron assay (IC50 47.35 μg/mL); chemiluminescence triggered by free radicals in the H2O2/horseradish peroxidase/luminol (IC50 0.286 μg/mL) and xanthine/xanthine oxidase/luminol (IC50 0.42 μg/mL) methods. CVE (10–100 mg per kg, 30 min before and immediately after UVB exposure) treatment was performed by gavage in hairless mice. CVE inhibited skin edema, neutrophil infiltration, and overproduction of MMP-9; reduced levels of TNF-α, IL-1β, and IL- 6; numbers of skin mast cells, epidermal thickening, number of epidermal apoptotic keratinocytes, and collagen degradation. CVE increased the skin's natural antioxidant defenses as observed by Nrf-2, NAD(P)H quinone oxidoreductase 1, and heme oxygenase 1 mRNA expression enhancement. Furthermore, CVE inhibited lipid peroxidation and superoxide anion production and recovered antioxidant reduced glutathione, catalase activity, and ROS scavenging capacity of the skin. Concluding, CVE downregulates the skin inflammatory and oxidative damages triggered by UVB, demonstrating its potentialities as a therapeutic approach.

ACS Style

Cristina P.B. Melo; Priscila Saito; David L. Vale; Camilla C.A. Rodrigues; Ingrid C. Pinto; Renata M. Martinez; Julia Rojo Bezerra; Marcela M. Baracat; Waldiceu A. Verri; Yris Maria Fonseca-Bazzo; Sandra R. Georgetti; Rubia Casagrande. Protective effect of oral treatment with Cordia verbenacea extract against UVB irradiation deleterious effects in the skin of hairless mouse. Journal of Photochemistry and Photobiology B: Biology 2021, 216, 112151 .

AMA Style

Cristina P.B. Melo, Priscila Saito, David L. Vale, Camilla C.A. Rodrigues, Ingrid C. Pinto, Renata M. Martinez, Julia Rojo Bezerra, Marcela M. Baracat, Waldiceu A. Verri, Yris Maria Fonseca-Bazzo, Sandra R. Georgetti, Rubia Casagrande. Protective effect of oral treatment with Cordia verbenacea extract against UVB irradiation deleterious effects in the skin of hairless mouse. Journal of Photochemistry and Photobiology B: Biology. 2021; 216 ():112151.

Chicago/Turabian Style

Cristina P.B. Melo; Priscila Saito; David L. Vale; Camilla C.A. Rodrigues; Ingrid C. Pinto; Renata M. Martinez; Julia Rojo Bezerra; Marcela M. Baracat; Waldiceu A. Verri; Yris Maria Fonseca-Bazzo; Sandra R. Georgetti; Rubia Casagrande. 2021. "Protective effect of oral treatment with Cordia verbenacea extract against UVB irradiation deleterious effects in the skin of hairless mouse." Journal of Photochemistry and Photobiology B: Biology 216, no. : 112151.

Immunology
Published: 26 January 2021 in Frontiers in Immunology
Reads 0
Downloads 0

The neglected tropical infirmity Chagas disease (CD) presents high mortality. Its etiological agent T. cruzi is transmitted by infected hematophagous insects. Symptoms of the acute phase of the infection include fever, fatigue, body aches, and headache, making diagnosis difficult as they are present in other illnesses as well. Thus, in endemic areas, individuals with undetermined pain may be considered for CD. Although pain is a characteristic symptom of CD, its cellular and molecular mechanisms are unknown except for demonstration of a role for peripheral TNF-α in CD pain. In this study, we evaluate the role of spinal cord glial cells in experimental T. cruzi infection in the context of pain using C57BL/6 mice. Pain, parasitemia, survival, and glial and neuronal function as well as NFκB activation and cytokine/chemokine production were assessed. T. cruzi infection induced chronic mechanical and thermal hyperalgesia. Systemic TNF-α and IL-1β peaked 14 days postinfection (p.i.). Infected mice presented increased spinal gliosis and NFκB activation compared to uninfected mice at 7 days p.i. Glial and NFκB inhibitors limited T. cruzi–induced pain. Nuclear phosphorylated NFκB was detected surrounded by glia markers, and glial inhibitors reduced its detection. T. cruzi–induced spinal cord production of cytokines/chemokines was also diminished by glial inhibitors. Dorsal root ganglia (DRG) neurons presented increased activity in infected mice, and the production of inflammatory mediators was counteracted by glial/NFκB inhibitors. The present study unveils the contribution of DRG and spinal cord cellular and molecular events leading to pain in T. cruzi infection, contributing to a better understanding of CD pathology.

ACS Style

Sergio M. Borghi; Victor Fattori; Thacyana T. Carvalho; Vera L. H. Tatakihara; Tiago H. Zaninelli; Felipe A. Pinho-Ribeiro; Camila R. Ferraz; Larissa Staurengo-Ferrari; Rubia Casagrande; Wander R. Pavanelli; Fernando Q. Cunha; Thiago M. Cunha; Phileno Pinge-Filho; Waldiceu A. Verri. Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NFκB Activation and Cytokine Production. Frontiers in Immunology 2021, 11, 1 .

AMA Style

Sergio M. Borghi, Victor Fattori, Thacyana T. Carvalho, Vera L. H. Tatakihara, Tiago H. Zaninelli, Felipe A. Pinho-Ribeiro, Camila R. Ferraz, Larissa Staurengo-Ferrari, Rubia Casagrande, Wander R. Pavanelli, Fernando Q. Cunha, Thiago M. Cunha, Phileno Pinge-Filho, Waldiceu A. Verri. Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NFκB Activation and Cytokine Production. Frontiers in Immunology. 2021; 11 ():1.

Chicago/Turabian Style

Sergio M. Borghi; Victor Fattori; Thacyana T. Carvalho; Vera L. H. Tatakihara; Tiago H. Zaninelli; Felipe A. Pinho-Ribeiro; Camila R. Ferraz; Larissa Staurengo-Ferrari; Rubia Casagrande; Wander R. Pavanelli; Fernando Q. Cunha; Thiago M. Cunha; Phileno Pinge-Filho; Waldiceu A. Verri. 2021. "Experimental Trypanosoma cruzi Infection Induces Pain in Mice Dependent on Early Spinal Cord Glial Cells and NFκB Activation and Cytokine Production." Frontiers in Immunology 11, no. : 1.

Review article
Published: 25 November 2020 in Immunology Letters
Reads 0
Downloads 0

Clinically, a variety of micro-organisms cause painful infections. Before seen as bystanders in the context of infections, recent studies have demonstrated that, as immune cells, nociceptors can sense pathogen-derived products. Nociceptors and immune cells, therefore, have evolved to communicate with each other to control inflammatory and host responses against pathogens in a complementary way. This interaction is named as neuroimmune communication (or axon-axon immune reflex) and initiates after the release of neuropeptides, such as CGRP and VIP by neurons. By this neurogenic response, nociceptors orchestrate the activity of innate and adaptive immune cells in a context-dependent manner. In this review, we focus on how nociceptors sense pathogen-derived products to shape the host response. We also highlight the new concept involving the resolution of inflammation, which is related to an active and time-dependent biosynthetic shift from pro-inflammatory to pro-resolution mediators, the so-called specialized pro-resolving lipid mediators (SPMs). At very low doses, SPMs act on specific receptors to silence nociceptors, limit pain and neurogenic responses, and resolve infections. Furthermore, stimulation of the vagus nerve induces SPMs production to regulate immune responses in infections. Therefore, harnessing the current understanding of neuro-immune communication and neurogenic responses might provide the bases for reprogramming host responses against infections through well balanced and effective immune response and inflammation resolution.

ACS Style

Victor Fattori; Camila R. Ferraz; Fernanda S. Rasquel-Oliveira; Waldiceu A. Verri. Neuroimmune communication in infection and pain: Friends or foes? Immunology Letters 2020, 229, 32 -43.

AMA Style

Victor Fattori, Camila R. Ferraz, Fernanda S. Rasquel-Oliveira, Waldiceu A. Verri. Neuroimmune communication in infection and pain: Friends or foes? Immunology Letters. 2020; 229 ():32-43.

Chicago/Turabian Style

Victor Fattori; Camila R. Ferraz; Fernanda S. Rasquel-Oliveira; Waldiceu A. Verri. 2020. "Neuroimmune communication in infection and pain: Friends or foes?" Immunology Letters 229, no. : 32-43.

Journal article
Published: 07 November 2020 in Chemico-Biological Interactions
Reads 0
Downloads 0

Neutrophil infiltration, pro-inflammatory cytokines, and reactive oxygen species (ROS) production have been implicated in development and progression of ulcerative colitis (UC), an inflammatory bowel disease (IBD) characterized by ulcerating inflammation of the mucosal layer generally restricted to the colon. The side effects, safety and human intolerance are limitations of the currently approved treatments for UC. Hesperidin methyl chalcone (HMC) is a flavonoid used to treat chronic venous disease, which shows anti-inflammatory, analgesic, and antioxidant properties in pre-clinical studies, however, its effects on colitis have never been described. Therefore, we aimed to evaluate the protective effects of HMC in a mouse model of acetic acid-induced colitis. Treatment with HMC significantly reduced neutrophil infiltration, edema, colon shortening, macro and microscopic damages induced by intracolonic administration of acetic acid. The improvement of colitis after HMC treatment is related to the increase in colon antioxidant status, and the inhibition of pro-inflammatory cytokines TNF-α, IL-6, IL-1β, and IL-33 in the colon. We observed, moreover, that HMC inhibited NF-κB activation in the colon, which might explain the reduction of the cytokines we observed. Finally, these results demonstrate a novel applicability of HMC to increase antioxidant response and reduce inflammation during acetic acid-induced colitis suggesting it as a promising therapeutic approach for the treatment of ulcerative colitis.

ACS Style

Carla F.S. Guazelli; Victor Fattori; Camila R. Ferraz; Sergio M. Borghi; Rubia Casagrande; Marcela M. Baracat; Waldiceu A. Verri. Antioxidant and anti-inflammatory effects of hesperidin methyl chalcone in experimental ulcerative colitis. Chemico-Biological Interactions 2020, 333, 109315 .

AMA Style

Carla F.S. Guazelli, Victor Fattori, Camila R. Ferraz, Sergio M. Borghi, Rubia Casagrande, Marcela M. Baracat, Waldiceu A. Verri. Antioxidant and anti-inflammatory effects of hesperidin methyl chalcone in experimental ulcerative colitis. Chemico-Biological Interactions. 2020; 333 ():109315.

Chicago/Turabian Style

Carla F.S. Guazelli; Victor Fattori; Camila R. Ferraz; Sergio M. Borghi; Rubia Casagrande; Marcela M. Baracat; Waldiceu A. Verri. 2020. "Antioxidant and anti-inflammatory effects of hesperidin methyl chalcone in experimental ulcerative colitis." Chemico-Biological Interactions 333, no. : 109315.

Original article
Published: 05 November 2020 in Mycopathologia
Reads 0
Downloads 0

Candida tropicalis is an important human pathogen that can undergo multiple forms of phenotypic switching. We aimed to evaluate the effect of phenotypic switching on the adhesion ability of C. tropicalis. C. tropicalis morphotypes included parental phenotypes (clinical isolates) and switch phenotypes (crepe, revertant of crepe—CR, rough, revertant of rough—RR, irregular center and revertant of irregular center—ICR). Adhesion to polystyrene and HeLa cells was determined by crystal violet assay. The percentage of HeLa cells with adhered yeasts and the number of adhered yeasts per HeLa cell were determined by light microscopy. Filamentation among adhered cells was assessed by direct counting. On polystyrene, 60% of the switch strains showed difference (p < 0.05) on adhesion ability compared to their parental counterpart strains, and altered thickness of adhered cells layers. Filamentation was increased among adhered cells of the switched strains compared to parental strains. A positive correlation was observed between adhesion on polystyrene and filamentation for morphotypes of the system 49.07. The majority of the switched strains showed higher adhesion capability to HeLa cells in comparison to the adherence of the clinical strains. All revertant strains showed a higher number of yeast cells per HeLa cell compared to their variant counterparts (p < 0.05), with exception of the ICR. Our findings indicate that switching events in C. tropicalis affect adhesion and filamentation of adhered cells on polystyrene and HeLa cells. The rise of switch strains with increased adhesion ability may contribute to the success of infection associated with C. tropicalis.

ACS Style

Cássia Milena de Souza; Hugo Felix Perini; Waldiceu Aparecido Verri; Tiago Henrique Zaninelli; Luciana Furlaneto-Maia; Marcia Cristina Furlaneto. Changes in Adhesion of Candida tropicalis Clinical Isolates Exhibiting Switch Phenotypes to Polystyrene and HeLa Cells. Mycopathologia 2020, 186, 81 -91.

AMA Style

Cássia Milena de Souza, Hugo Felix Perini, Waldiceu Aparecido Verri, Tiago Henrique Zaninelli, Luciana Furlaneto-Maia, Marcia Cristina Furlaneto. Changes in Adhesion of Candida tropicalis Clinical Isolates Exhibiting Switch Phenotypes to Polystyrene and HeLa Cells. Mycopathologia. 2020; 186 (1):81-91.

Chicago/Turabian Style

Cássia Milena de Souza; Hugo Felix Perini; Waldiceu Aparecido Verri; Tiago Henrique Zaninelli; Luciana Furlaneto-Maia; Marcia Cristina Furlaneto. 2020. "Changes in Adhesion of Candida tropicalis Clinical Isolates Exhibiting Switch Phenotypes to Polystyrene and HeLa Cells." Mycopathologia 186, no. 1: 81-91.

Journal article
Published: 09 October 2020 in Phytomedicine
Reads 0
Downloads 0

: Hepatocellular Carcinoma (HCC) is extremely aggressive and presents low rates of response to the available chemotherapeutic agents. Many studies have focused on the search for alternative low-cost natural compounds with antiproliferative potential that selectively respond to liver cancer cells. : This study assessed the in vitro direct action of trans-chalcone (TC) on cells of the human HCC HuH7.5 cell line. : We subjected the HuH7.5 tumor cells to TC treatment at increasing concentrations (12.5-100 µM) for 24 and 48 h. Cell viability was verified through MTT and 50% inhibitory concentration of cells (IC50 23.66 µM) was determined within 48 h. We quantified trypan blue proliferation and light microscopy, ROS production, mitochondrial depolarization and autophagy, cell cycle analysis, and apoptosis using Muse® cell analyzer and immunocytochemical markings of p-p53 and β-catenin. : Data showed an effective dose- and time-dependent TC-cytotoxic action at low micromolar concentrations without causing toxicity to non-cancerous cells, such as erythrocytes. TC-treatment caused mitochondrial membrane damage and cell cycle G0/G1 phase arrest, increasing the presence of the p53 protein and decreasing β-catenin, in addition, to inducing cell death by autophagy. Additionally, TC decreased the metastatic capacity of HuH7.5, which affected the migration/invasion of this type of cell. : In vitro trans-chalcone activity in the human HCC HuH7.5 tumor cell line is shown to be a potential molecule to develop new therapies to repair the p53 pathway and prevent the overexpression of Wnt/β-catenin tumor development inducing autophagy cell death and decreasing metastatic capacity of HuH7.5 cell line.

ACS Style

Elaine Da Silva Siqueira; Vírgínia Márcia Concato; Fernanda Tomiotto-Pellissier; Taylon Felipe Silva; Bruna Taciane Da Silva Bortoleti; Manoela Daiele Gonçalves; Idessania Nazareth Costa; Waldiceu Aparecido Verri Junior; Wander Rogério Pavanelli; Carolina Panis; Mário Sérgio Mantovani; Milena Menegazzo Miranda-Sapla; Ivete Conchon-Costa. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine 2020, 80, 153373 .

AMA Style

Elaine Da Silva Siqueira, Vírgínia Márcia Concato, Fernanda Tomiotto-Pellissier, Taylon Felipe Silva, Bruna Taciane Da Silva Bortoleti, Manoela Daiele Gonçalves, Idessania Nazareth Costa, Waldiceu Aparecido Verri Junior, Wander Rogério Pavanelli, Carolina Panis, Mário Sérgio Mantovani, Milena Menegazzo Miranda-Sapla, Ivete Conchon-Costa. Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line. Phytomedicine. 2020; 80 ():153373.

Chicago/Turabian Style

Elaine Da Silva Siqueira; Vírgínia Márcia Concato; Fernanda Tomiotto-Pellissier; Taylon Felipe Silva; Bruna Taciane Da Silva Bortoleti; Manoela Daiele Gonçalves; Idessania Nazareth Costa; Waldiceu Aparecido Verri Junior; Wander Rogério Pavanelli; Carolina Panis; Mário Sérgio Mantovani; Milena Menegazzo Miranda-Sapla; Ivete Conchon-Costa. 2020. "Trans-chalcone induces death by autophagy mediated by p53 up-regulation and β-catenin down-regulation on human hepatocellular carcinoma HuH7.5 cell line." Phytomedicine 80, no. : 153373.

Original article
Published: 01 October 2020 in Brazilian Journal of Psychiatry
Reads 0
Downloads 0

This randomized controlled trial examined the efficacy and safety of N-acetylcysteine as an adjunctive treatment for smoking cessation. Heavy smokers were recruited from smoking cessation treatment for this 12- week randomized controlled trial. Eligible tobacco use disorder outpatients (n=34) were randomized to N-acetylcysteine or placebo plus first-line treatment. Abstinence was verified by exhaled carbon monoxide (COexh). The assessment scales included the Fagerström Test for Nicotine Dependence, the Hamilton Depression Rating Scale, the Hamilton Anxiety Rating Scale, the Minnesota Nicotine Withdrawal Scale, and the Medication Adherence Rating Scale. We also assessed anthropometrics, blood pressure, lipid profile, and soluble tumor necrosis factor receptor (sTNF-R) levels 1 and 2. First-line treatment for smoking cessation plus adjunctive N-acetylcysteine or placebo significantly reduced COexh (p < 0.01). In the N-acetylcysteine group, no significant changes were found in nicotine withdrawal symptoms, depressive and anxiety symptoms, anthropometric measures, blood pressure, or glucose compared to placebo. However, there was a significant reduction in sTNF-R2 levels between baseline and week 12 in the N-acetylcysteine group. These findings highlight the need to associate N-acetylcysteine with first-line treatment for smoking cessation, since combined treatment may affect inflammation and metabolism components. NCT02420418

ACS Style

Regina C.B.R. Machado; Heber O. Vargas; Marcela M. Baracat; Mariana R. Urbano; Waldiceu A. Verri Jr; Mauro Porcu; Sandra O.V. Nunes. N-acetylcysteine as an adjunctive treatment for smoking cessation: a randomized clinical trial. Brazilian Journal of Psychiatry 2020, 42, 519 -526.

AMA Style

Regina C.B.R. Machado, Heber O. Vargas, Marcela M. Baracat, Mariana R. Urbano, Waldiceu A. Verri Jr, Mauro Porcu, Sandra O.V. Nunes. N-acetylcysteine as an adjunctive treatment for smoking cessation: a randomized clinical trial. Brazilian Journal of Psychiatry. 2020; 42 (5):519-526.

Chicago/Turabian Style

Regina C.B.R. Machado; Heber O. Vargas; Marcela M. Baracat; Mariana R. Urbano; Waldiceu A. Verri Jr; Mauro Porcu; Sandra O.V. Nunes. 2020. "N-acetylcysteine as an adjunctive treatment for smoking cessation: a randomized clinical trial." Brazilian Journal of Psychiatry 42, no. 5: 519-526.

Original research paper
Published: 04 September 2020 in Inflammation Research
Reads 0
Downloads 0

To investigate the role of IL-33 in gouty arthritis. 174 Balb/c (wild-type) and 54 ST2−/− mice were used in this study. In vitro experiments were conducted in bone marrow-derived macrophages (BMDMs). Synovial fluid samples from gouty arthritis (n = 7) and osteoarthritis (n = 8) hospital patients were used to measure IL-33 and sST2 levels. Gout was induced by injection of monosodium urate (MSU) crystals in the knee joint of mice. Pain was determined using the electronic von Frey and static weight bearing. Neutrophil recruitment was determined by H&E staining, Rosenfeld staining slides, and MPO activity. ELISA was used for cytokine and sST2 measurement. The priming effect of IL-33 was determined in BMDM. Synovial fluid of gout patients showed higher IL-33 levels and neutrophil counts than osteoarthritis patients. In mice, the absence of ST2 prevented mechanical pain, knee joint edema, neutrophil recruitment to the knee joint, and lowered IL-1β and superoxide anion levels. In macrophages, IL-33 enhanced the release of IL-1β and TNF-α, and BMDMs from ST2−/− showed reduced levels of these cytokines after stimulus with MSU crystals. IL-33 mediates gout pain and inflammation by boosting macrophages production of cytokines upon MSU crystals stimulus.

ACS Style

Victor Fattori; Larissa Staurengo-Ferrari; Tiago H. Zaninelli; Rubia Casagrande; Rene D. Oliveira; Paulo Louzada-Junior; Thiago M. Cunha; Jose C. Alves-Filho; Mauro M. Teixeira; Fernando Q. Cunha; Flavio A. Amaral; Waldiceu A. Verri. IL-33 enhances macrophage release of IL-1β and promotes pain and inflammation in gouty arthritis. Inflammation Research 2020, 69, 1271 -1282.

AMA Style

Victor Fattori, Larissa Staurengo-Ferrari, Tiago H. Zaninelli, Rubia Casagrande, Rene D. Oliveira, Paulo Louzada-Junior, Thiago M. Cunha, Jose C. Alves-Filho, Mauro M. Teixeira, Fernando Q. Cunha, Flavio A. Amaral, Waldiceu A. Verri. IL-33 enhances macrophage release of IL-1β and promotes pain and inflammation in gouty arthritis. Inflammation Research. 2020; 69 (12):1271-1282.

Chicago/Turabian Style

Victor Fattori; Larissa Staurengo-Ferrari; Tiago H. Zaninelli; Rubia Casagrande; Rene D. Oliveira; Paulo Louzada-Junior; Thiago M. Cunha; Jose C. Alves-Filho; Mauro M. Teixeira; Fernando Q. Cunha; Flavio A. Amaral; Waldiceu A. Verri. 2020. "IL-33 enhances macrophage release of IL-1β and promotes pain and inflammation in gouty arthritis." Inflammation Research 69, no. 12: 1271-1282.

Original article
Published: 30 June 2020 in Journal of Drug Targeting
Reads 0
Downloads 0

The use of compounds from natural or synthetic sources and nanotechnology may represent an alternative to develop new drugs for the leishmaniasis treatment. DETC is an inhibitor of the SOD1 enzyme, which leads to increased ROS production, important for the elimination of Leishmania. Thus, our objective was to assess the leishmanicidal in vitro effect of free Diethydithiocarbamate (DETC) and DETC loaded in beeswax-copaiba oil nanoparticles (DETC-Beeswax-CO Nps) on L. amazonensis forms and elucidate the possible mechanisms involved in the parasite death. DETC-Beeswax-CO Nps presented size below 200 nm, spherical morphology, negative zeta potential, and high encapsulation efficiency. Free DETC reduced the viability of promastigotes and increase ROS production, lower the mitochondrial membrane potential, cause phosphatidylserine exposure, and enhance plasma membrane permeability, in addition to promoting morphological changes in the parasite. Free DETC proved toxic in the assessment of toxicity to murine macrophages, however, the encapsulation of this compound was able to reduce these toxic effects on macrophages. DETC-Beeswax-CO Nps exerted anti-amastigote effect by enhancing the production of ROS, superoxide anion, TNF-α, IL-6, and reduced IL-10 in macrophages. Therefore, free DETC induces antipromastigote effect by apoptosis-like; and DETC-Beeswax-CO Nps exerted anti-leishmanial effect due to pro-oxidant and pro-inflammatory response.

ACS Style

João Paulo Assolini; Fernanda Tomiotto-Pellissier; Bruna Taciane Da Silva Bortoleti; Manoela Daiele Gonçalves; Claudia Stoeglehner Sahd; Amanda Cristina Machado Carloto; Paulo Emilio Feuser; Arthur Poester Cordeiro; Sergio Marques Borghi; Waldiceu Aparecido Verri Jr; Claudia Sayer; Pedro Henrique Hermes De Araújo; Idessania Nazareth Costa; Ivete Conchon-Costa; Milena Menegazzo Miranda-Sapla; Wander Rogério Pavanelli. Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage. Journal of Drug Targeting 2020, 28, 1110 -1123.

AMA Style

João Paulo Assolini, Fernanda Tomiotto-Pellissier, Bruna Taciane Da Silva Bortoleti, Manoela Daiele Gonçalves, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Sergio Marques Borghi, Waldiceu Aparecido Verri Jr, Claudia Sayer, Pedro Henrique Hermes De Araújo, Idessania Nazareth Costa, Ivete Conchon-Costa, Milena Menegazzo Miranda-Sapla, Wander Rogério Pavanelli. Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage. Journal of Drug Targeting. 2020; 28 (10):1110-1123.

Chicago/Turabian Style

João Paulo Assolini; Fernanda Tomiotto-Pellissier; Bruna Taciane Da Silva Bortoleti; Manoela Daiele Gonçalves; Claudia Stoeglehner Sahd; Amanda Cristina Machado Carloto; Paulo Emilio Feuser; Arthur Poester Cordeiro; Sergio Marques Borghi; Waldiceu Aparecido Verri Jr; Claudia Sayer; Pedro Henrique Hermes De Araújo; Idessania Nazareth Costa; Ivete Conchon-Costa; Milena Menegazzo Miranda-Sapla; Wander Rogério Pavanelli. 2020. "Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage." Journal of Drug Targeting 28, no. 10: 1110-1123.

Journal article
Published: 26 June 2020 in Molecules
Reads 0
Downloads 0

Excessive exposure to UV, especially UVB, is the most important risk factor for skin cancer and premature skin aging. The identification of the specialized pro-resolving lipid mediators (SPMs) challenged the preexisting paradigm of how inflammation ends. Rather than a passive process, the resolution of inflammation relies on the active production of SPMs, such as Lipoxins (Lx), Maresins, protectins, and Resolvins. LXA4 is an SPM that exerts its action through ALX/FPR2 receptor. Stable ALX/FPR2 agonists are required because SPMs can be quickly metabolized within tissues near the site of formation. BML-111 is a commercially available synthetic ALX/FPR2 receptor agonist with analgesic, antioxidant, and anti-inflammatory properties. Based on that, we aimed to determine the effect of BML-111 in a model of UVB-induced skin inflammation in hairless mice. We demonstrated that BML-111 ameliorates the signs of UVB-induced skin inflammation by reducing neutrophil recruitment and mast cell activation. Reduction of these cells by BML-111 led to lower number of sunburn cells formation, decrease in epidermal thickness, collagen degradation, cytokine production (TNF-α, IL-1β, IL-6, TGF, and IL-10), and oxidative stress (observed by an increase in total antioxidant capacity and Nrf2 signaling pathway), indicating that BML-111 might be a promising drug to treat skin disorders.

ACS Style

Renata M. Martinez; Victor Fattori; Priscila Saito; Ingrid C. Pinto; Camilla C. A. Rodrigues; Cristina P. B. Melo; Allan J. C. Bussmann; Larissa Staurengo-Ferrari; Julia Rojo Bezerra; Josiane A. Vignoli; Marcela M. Baracat; Sandra R. Georgetti; Waldiceu Verri; Rubia Casagrande; Waldiceu A Verri Jr.. The Lipoxin Receptor/FPR2 Agonist BML-111 Protects Mouse Skin Against Ultraviolet B Radiation. Molecules 2020, 25, 2953 .

AMA Style

Renata M. Martinez, Victor Fattori, Priscila Saito, Ingrid C. Pinto, Camilla C. A. Rodrigues, Cristina P. B. Melo, Allan J. C. Bussmann, Larissa Staurengo-Ferrari, Julia Rojo Bezerra, Josiane A. Vignoli, Marcela M. Baracat, Sandra R. Georgetti, Waldiceu Verri, Rubia Casagrande, Waldiceu A Verri Jr.. The Lipoxin Receptor/FPR2 Agonist BML-111 Protects Mouse Skin Against Ultraviolet B Radiation. Molecules. 2020; 25 (12):2953.

Chicago/Turabian Style

Renata M. Martinez; Victor Fattori; Priscila Saito; Ingrid C. Pinto; Camilla C. A. Rodrigues; Cristina P. B. Melo; Allan J. C. Bussmann; Larissa Staurengo-Ferrari; Julia Rojo Bezerra; Josiane A. Vignoli; Marcela M. Baracat; Sandra R. Georgetti; Waldiceu Verri; Rubia Casagrande; Waldiceu A Verri Jr.. 2020. "The Lipoxin Receptor/FPR2 Agonist BML-111 Protects Mouse Skin Against Ultraviolet B Radiation." Molecules 25, no. 12: 2953.

Research article
Published: 04 June 2020 in Human & Experimental Toxicology
Reads 0
Downloads 0

Malathion is an organophosphate pesticide widely used for agricultural crops and for vector control of Aedes aegypti. Humans are exposed to this environmental contaminant by ingesting contaminated food. The juvenile and peripubertal periods are critical for the postnatal development of the epididymis and are when animals are most vulnerable to toxic agents. Since juveniles and adolescents are developing under exposure to the insecticide malathion, the aim of the present study was to evaluate the effects of exposure to low doses of malathion on postnatal epididymal development in rats. Male Wistar rats were exposed to malathion daily via gavage at doses of 10 mg kg−1 (M10 group) or 50 mg kg−1 (M50 group) for 40 days (postnatal days (PNDs) 25–65). The control group received the vehicle (0.9% saline) under the same conditions. On PND 40, the epididymides were removed, weighed and used for histological analysis and determination of the inflammatory profile and sperm count. Sperm from the vas deferens were subjected to sperm motility analysis. The M50 group showed tissue remodelling in the caput and cauda epididymides and increased neutrophil and macrophage migration in the caput epididymis. The M10 group showed decreased motile spermatozoa and IL-6 levels in the caput epididymis. Both doses decreased the IL-1β level and altered the morphology of the same region. These results show that malathion exposure may impair postnatal epididymal development. Furthermore, alterations of the immune system in the epididymal environment are presented as new findings regarding the action of malathion on the epididymis.

ACS Style

R P Erthal; Geml Siervo; L Staurengo-Ferrari; Victor Fattori; R R Pescim; Waldiceu Verri; Glaura Fernandes. Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats. Human & Experimental Toxicology 2020, 39, 1487 -1496.

AMA Style

R P Erthal, Geml Siervo, L Staurengo-Ferrari, Victor Fattori, R R Pescim, Waldiceu Verri, Glaura Fernandes. Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats. Human & Experimental Toxicology. 2020; 39 (11):1487-1496.

Chicago/Turabian Style

R P Erthal; Geml Siervo; L Staurengo-Ferrari; Victor Fattori; R R Pescim; Waldiceu Verri; Glaura Fernandes. 2020. "Impairment of postnatal epididymal development and immune microenvironment following administration of low doses of malathion during juvenile and peripubertal periods of rats." Human & Experimental Toxicology 39, no. 11: 1487-1496.

Journal article
Published: 30 May 2020 in Reproductive Toxicology
Reads 0
Downloads 0

Malathion is an organophosphate insecticide used in agriculture and for controlling vector-borne diseases such as Zika. Humans can be exposed to malathion by means of ingestion of contaminated food. The juvenile and peripubertal periods are a large window of vulnerability to the action of toxic agents. The aim of the present study was to evaluate the effects of low doses of malathion during the development of testes in the juvenile and peripubertal periods in rats. For this purpose, 45 male Wistar rats (postnatal day (PND) 25) were assigned to 3 experimental groups and treated for 40 days. The animals were exposed daily to malathion 10 mg/kg (M10 group) or 50 mg/kg (M50 group) diluted in 0.9 % saline via gavage. The control group received only the vehicle. On the 40th experimental day, the rats were anaesthetized and euthanized. The blood was collected for determination of testosterone concentration. The testes were removed and weighed. Spermatozoa from the vas deferens were used for sperm morphological analysis. The testes were used for evaluation of sperm count and oxidative stress status to determine the inflammatory profile and analysis of tissue constitution. The results showed that both malathion doses reduced the sperm count and increased the number of abnormal sperms. Furthermore, both doses altered the spermatogenetic process, delayed spermiogenesis, reduced the Leydig and Sertoli cell number and increased the thickness of tunica albuginea. The M10 group presented increased IL-10 levels and reduced GSH levels. These parameters did not change in the M50 group. However, the M50 group showed an increase in the number of abnormal seminiferous tubules, a decrease in plasma testosterone concentration and an increase in lipid peroxidation in the testes. In conclusion, the exposure to low doses of malathion during juvenile and peripubertal development resulted in testicular toxicity and compromised the testicular morphology and function.

ACS Style

Rafaela Pires Erthal; Larissa Staurengo-Ferrari; Victor Fattori; Karen Gomes Luiz; Fernando Queiroz Cunha; Rodrigo Rosseto Pescim; Rubens Cecchini; Waldiceu Aparecido Verri; Flavia Alessandra Guarnier; Glaura Scantamburlo Alves Fernandes. Exposure to low doses of malathion during juvenile and peripubertal periods impairs testicular and sperm parameters in rats: Role of oxidative stress and testosterone. Reproductive Toxicology 2020, 96, 17 -26.

AMA Style

Rafaela Pires Erthal, Larissa Staurengo-Ferrari, Victor Fattori, Karen Gomes Luiz, Fernando Queiroz Cunha, Rodrigo Rosseto Pescim, Rubens Cecchini, Waldiceu Aparecido Verri, Flavia Alessandra Guarnier, Glaura Scantamburlo Alves Fernandes. Exposure to low doses of malathion during juvenile and peripubertal periods impairs testicular and sperm parameters in rats: Role of oxidative stress and testosterone. Reproductive Toxicology. 2020; 96 ():17-26.

Chicago/Turabian Style

Rafaela Pires Erthal; Larissa Staurengo-Ferrari; Victor Fattori; Karen Gomes Luiz; Fernando Queiroz Cunha; Rodrigo Rosseto Pescim; Rubens Cecchini; Waldiceu Aparecido Verri; Flavia Alessandra Guarnier; Glaura Scantamburlo Alves Fernandes. 2020. "Exposure to low doses of malathion during juvenile and peripubertal periods impairs testicular and sperm parameters in rats: Role of oxidative stress and testosterone." Reproductive Toxicology 96, no. : 17-26.