Drought is one of the greatest limitations to crop expansion outside the present-day agricultural areas. It will become increasingly important in regions of the globe where, in the past, the problem was negligible, due to the recognized changes in global climate. Today the concern is with improving cultural practices and crop genotypes for drought-prone areas; therefore, understanding the mechanisms behind drought resistance and the efficient use of water by the plants is fundamental for the achievement of those goals. In this paper, the major constraints to carbon assimilation and the metabolic regulations that play a role in plant responses to water deficits, acting in isolation or in conjunction with other stresses, is reviewed. The effects on carbon assimilation include increased resistance to diffusion by stomata and the mesophyll, as well as biochemical and photochemical adjustments. Oxidative stress is critical for crops that experience drought episodes. The role of detoxifying systems in preventing irreversible damage to photosynthetic machinery and of redox molecules as local or systemic signals is revised. Plant capacity to avoid or repair membrane damage during dehydration and rehydration processes is pivotal for the maintenance of membrane integrity, especially for those that embed functional proteins. Among such proteins are water transporters, whose role in the regulation of plant water status and transport of other metabolites is the subject of intense investigation. Long-distance chemical signalling, as an early response to drought, started to be unravelled more than a decade ago. The effects of those signals on carbon assimilation and partitioning of assimilates between reproductive and non-reproductive structures are revised and discussed in the context of novel management techniques. These applications are designed to combine increased crop water-use efficiency with sustained yield and improved quality of the products. Through an understanding of the mechanisms leading to successful adaptation to dehydration and rehydration, it has already been possible to identify key genes able to alter metabolism and increase plant tolerance to drought. An overview of the most important data on this topic, including engineering for osmotic adjustment or protection, water transporters, and C₄ traits is presented in this paper. Emphasis is given to the most successful or promising cases of genetic engineering in crops, using functional or regulatory genes. as well as to promising technologies, such as the transfer of transcription factors.

Soil salinity is a major abiotic stress that results in considerable crop yield losses worldwide. However, some plant genotypes show a high tolerance to soil salinity, as they manage to maintain a high K⁺/Na⁺ ratio in the cytosol, in contrast to salt stress susceptible genotypes. Although, different plant genotypes show different salt tolerance mechanisms, they all rely on the regulation and function of K⁺ and Na⁺ transporters and H⁺ pumps, which generate the driving force for K⁺ and Na⁺ transport. In this review we will introduce salt stress responses in plants and summarize the current knowledge about the most important ion transporters that facilitate intra- and intercellular K⁺ and Na⁺ homeostasis in these organisms. We will describe and discuss the regulation and function of the H⁺-ATPases, H⁺-PPases, SOS1, HKTs, and NHXs, including the specific tissues where they work and their response to salt stress.

Environmental conditions, such as water supply, temperature and salinity, strongly affect plant growth and development. Extremes of these conditions (abiotic stresses) adversely affect many different mechanisms associated with plant responses and adaptation to stress: photosynthetic mechanisms, e.g. stomatal control of CO₂ diffusion, photosystem II repair, ribulose bisphosphate carboxylase/oxygenase (Rubisco) activity and scavenging of reactive oxygen species (ROS), are susceptible to damage, and photosynthetic efficiency can be greatly decreased. Responses and adaptations require differential gene expression, which is regulated by specific transcription factors (TFs). The role and regulation of several TFs involved in abiotic stress response pathways are considered, with emphasis on new findings regarding expression of genes related to both stomatal and non-stomatal limitations to CO₂ photosynthetic assimilation. Many TFs, belonging to different families (e.g. MYB, bZIP and DREB), have been related to abiotic stress responses; however, only a few are known to regulate the expression of photosynthesis-related genes in response to stress. Several TFs belonging to the MYB family play an important role in both stomatal and non-stomatal responses by regulation of stomatal numbers and sizes, and metabolic components, respectively. To obtain more insight into this area of potentially large agronomic impact, it is essential to identify and functionally characterize new TFs that mediate the stress responses regulating the expression of genes associated with photosynthesis and related metabolism.