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Lihua Guo
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China

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Research article
Published: 03 April 2020 in Science Advances
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Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.

ACS Style

Pengfei Li; Shuangchao Wang; Lihang Zhang; Dewen Qiu; Xueping Zhou; Lihua Guo. A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions. Science Advances 2020, 6, eaay9634 .

AMA Style

Pengfei Li, Shuangchao Wang, Lihang Zhang, Dewen Qiu, Xueping Zhou, Lihua Guo. A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions. Science Advances. 2020; 6 (14):eaay9634.

Chicago/Turabian Style

Pengfei Li; Shuangchao Wang; Lihang Zhang; Dewen Qiu; Xueping Zhou; Lihua Guo. 2020. "A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions." Science Advances 6, no. 14: eaay9634.

Journal article
Published: 24 March 2020 in Viruses
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Fungal viruses (mycoviruses) have attracted more attention for their possible hypovirulence (attenuation of fungal virulence) trait, which may be developed as a biocontrol agent of plant pathogenic fungi. However, most discovered mycoviruses are asymptomatic in their hosts. In most cases, mycovirus hypovirulent factors have not been explored clearly. In this study, we characterized a ssRNA mycovirus in Fusarium graminearum strain HB56-9. The complete nucleotide genome was obtained by combining random sequencing and rapid amplification of cDNA ends (RACE). The full genome was 6621-nucleotides long, excluding the poly(A) tail. The mycovirus was quite interesting because it shared 95.91% nucleotide identities with previously reported Fusarium graminearum virus 1 strain DK21 (FgV1-DK21), while the colony morphology of their fungal hosts on PDA plates were very different. The novel virus was named Fusarium graminearum virus 1 Chinese isolate (FgV1-ch). Like FgV1-DK21, FgV1-ch also contains four putative open reading frames (ORFs), including one long and three short ORFs. A phylogenetic analysis indicated that FgV1-ch is clustered into a proposed family Fusariviridae. FgV1-ch, unlike FgV1-DK21, had mild or no effects on host mycelial growth, spore production and virulence. The nucleotide differences between FgV1-ch and FgV1-DK21 will help to elucidate the hypovirulence determinants during mycovirus–host interaction.

ACS Style

Lihang Zhang; Xiaoguang Chen; Pallab Bhattacharjee; Yue Shi; Lihua Guo; Shuangchao Wang. Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum. Viruses 2020, 12, 357 .

AMA Style

Lihang Zhang, Xiaoguang Chen, Pallab Bhattacharjee, Yue Shi, Lihua Guo, Shuangchao Wang. Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum. Viruses. 2020; 12 (3):357.

Chicago/Turabian Style

Lihang Zhang; Xiaoguang Chen; Pallab Bhattacharjee; Yue Shi; Lihua Guo; Shuangchao Wang. 2020. "Molecular Characterization of a Novel Strain of Fusarium graminearum Virus 1 Infecting Fusarium graminearum." Viruses 12, no. 3: 357.

Review
Published: 18 July 2019 in Frontiers in Cellular and Infection Microbiology
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Fusarium is an important genus of plant pathogenic fungi, and is widely distributed in soil and associated with plants worldwide. The diversity of mycoviruses in Fusarium is increasing continuously due to the development and extensive use of state-of-the-art RNA deep sequencing techniques. To date, fully-sequenced mycoviruses have been reported in 13 Fusarium species: Fusarium asiaticum, F. boothii, F. circinatum, F. coeruleum, F. globosum, F. graminearum, F. incarnatum, F. langsethiae, F. oxysporum, F. poae, F. pseudograminearum, F. solani, and F. virguliforme. Most Fusarium mycoviruses establish latent infections, but some mycoviruses such as Fusarium graminearum virus 1 (FgV1), Fusarium graminearum virus-ch9 (FgV-ch9), Fusarium graminearum hypovirus 2 (FgHV2), and Fusarium oxysporum f. sp. dianthi mycovirus 1 (FodV1) cause hypovirulence. Rapid advances in various omics technologies used to elucidate genes or biological processes can facilitate an improved understanding of mycovirus-host interactions. The review aims to illuminate the recent advances in studies of mycoviruses in Fusarium, including those related to diversity, molecular mechanisms of virus-host interaction. We also discuss the induction and suppression of RNA silencing including the role of RNAi components as an antiviral defense response.

ACS Style

Pengfei Li; Pallab Bhattacharjee; Shuangchao Wang; Lihang Zhang; Irfan Ahmed; Lihua Guo. Mycoviruses in Fusarium Species: An Update. Frontiers in Cellular and Infection Microbiology 2019, 9, 257 .

AMA Style

Pengfei Li, Pallab Bhattacharjee, Shuangchao Wang, Lihang Zhang, Irfan Ahmed, Lihua Guo. Mycoviruses in Fusarium Species: An Update. Frontiers in Cellular and Infection Microbiology. 2019; 9 ():257.

Chicago/Turabian Style

Pengfei Li; Pallab Bhattacharjee; Shuangchao Wang; Lihang Zhang; Irfan Ahmed; Lihua Guo. 2019. "Mycoviruses in Fusarium Species: An Update." Frontiers in Cellular and Infection Microbiology 9, no. : 257.

Original article
Published: 13 November 2018 in 3 Biotech
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The protein elicitor PeaT1 was found in Alternaria tenuissima and exerted broad spectrum resistance in wheat, cotton, and rice. Recently, we found that overexpressing PeaT1 rice (OE) could enhance plant drought tolerance. Elucidating some elevated drought stress-related proteins and associated mechanisms is inevitable for improving drought tolerance in rice. In this study, combining a label-free quantitative proteomic method, multiple proteins were differentially accumulated in OE plants. Among these, a total of 57 significant changed proteins (including 32 up-regulated and 25 down-regulated) were mainly involved in metabolic, cellular, biological progress, and stress response. Using the RT-qPCR assay, 18 proteins’ relative abundance was detected mostly consistent with the proteins abundance in proteomic data. Specially, proteins involved in abiotic stress, such as OsSKIPa and OsPP2C, which were significantly induced in early after dehydration treatment in transgenic rice, and the other stress response genes (prohibitin protein, PsbP protein, msrB Protein) also changed in PeaT1 OE lines. Taken together, these results suggested that these differential proteins would be helpful for understanding the functional molecular mechanism of PeaT1 in rice.

ACS Style

Fachao Shi; Xiufen Yang; Hongmei Zeng; Lihua Guo; Dewen Qiu. Label-free quantitative proteomic analysis revealed a positive effect of ectopic over-expression of PeaT1 from Alternaria tenuissima on rice (Oryza sativa) response to drought. 3 Biotech 2018, 8, 480 .

AMA Style

Fachao Shi, Xiufen Yang, Hongmei Zeng, Lihua Guo, Dewen Qiu. Label-free quantitative proteomic analysis revealed a positive effect of ectopic over-expression of PeaT1 from Alternaria tenuissima on rice (Oryza sativa) response to drought. 3 Biotech. 2018; 8 (11):480.

Chicago/Turabian Style

Fachao Shi; Xiufen Yang; Hongmei Zeng; Lihua Guo; Dewen Qiu. 2018. "Label-free quantitative proteomic analysis revealed a positive effect of ectopic over-expression of PeaT1 from Alternaria tenuissima on rice (Oryza sativa) response to drought." 3 Biotech 8, no. 11: 480.

Journal article
Published: 01 May 2018 in Virology
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Here we describe a novel (-)ssRNA mycovirus, Fusarium graminearum negative-stranded RNA virus 1 (FgNSRV-1), isolated from Fusarium graminearum strain HN1. The genome of FgNSRV-1 is 9072 nucleotides in length, with five discontinuous but linear ORFs (ORF I-V). Phylogenetic analysis based on entire L polymerase sequences indicated that FgNSRV-1 is related to the (-)ssRNA mycovirus Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), and other mycoviruses. Our data suggest that FgNSRV-1 can be classified into the family Mymonaviridae, order Mononegavirales. Putative enveloped virion-like structures with filamentous morphology similar to SsNSRV-1 were observed in virion preparation samples. The L proteins of FgNSRV-1, and other fungal mononegaviruses, were found to be related to L protein-like sequences in some fungal genome, supporting the hypothesis that there is coevolution occurring between mycoviruses and fungi. Besides, clearing the virus from the infected host fungus resulted in no discernable phenotypic change.

ACS Style

Luan Wang; Hao He; Shuangchao Wang; Xiaoguang Chen; Dewen Qiu; Hideki Kondo; Lihua Guo. Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum. Virology 2018, 518, 232 -240.

AMA Style

Luan Wang, Hao He, Shuangchao Wang, Xiaoguang Chen, Dewen Qiu, Hideki Kondo, Lihua Guo. Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum. Virology. 2018; 518 ():232-240.

Chicago/Turabian Style

Luan Wang; Hao He; Shuangchao Wang; Xiaoguang Chen; Dewen Qiu; Hideki Kondo; Lihua Guo. 2018. "Evidence for a novel negative-stranded RNA mycovirus isolated from the plant pathogenic fungus Fusarium graminearum." Virology 518, no. : 232-240.

Journal article
Published: 22 February 2018 in Genome Announcements
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We describe here a double-stranded RNA mycovirus, termed Fusarium graminearum alternavirus 1 (FgAV1/AH11), from the isolate AH11 of the phytopathogenic fungus F. graminearum . Phylogenetic analysis showed that FgAV1/AH11 belongs to a newly proposed family, Alternaviridae . This is the first report of a mycovirus in the family Alternaviridae that infects F. graminearum .

ACS Style

Hao He; Xiaoguang Chen; Pengfei Li; Dewen Qiu; Lihua Guo. Complete Genome Sequence of a Fusarium graminearum Double-Stranded RNA Virus in a Newly Proposed Family, Alternaviridae. Genome Announcements 2018, 6, 1 .

AMA Style

Hao He, Xiaoguang Chen, Pengfei Li, Dewen Qiu, Lihua Guo. Complete Genome Sequence of a Fusarium graminearum Double-Stranded RNA Virus in a Newly Proposed Family, Alternaviridae. Genome Announcements. 2018; 6 (8):1.

Chicago/Turabian Style

Hao He; Xiaoguang Chen; Pengfei Li; Dewen Qiu; Lihua Guo. 2018. "Complete Genome Sequence of a Fusarium graminearum Double-Stranded RNA Virus in a Newly Proposed Family, Alternaviridae." Genome Announcements 6, no. 8: 1.

Annotated sequence record
Published: 15 March 2017 in Archives of Virology
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The complete nucleotide sequence of a double-stranded RNA (dsRNA) mycovirus, Fusarium graminearum dsRNA virus 5 (FgV5), was identified and characterized. The FgV5 genome comprises two dsRNA genome segments of 2030 bp and 1740 bp. FgV5 dsRNA1 contains a single open reading frame (ORF1), which is predicted to encode a protein of 613 amino acids (aa) with a molecular mass of 70.4 kDa and has a conserved RNA-dependent RNA polymerase (RdRp) motif. FgV5 dsRNA2 is predicted to contain two discontinuous ORFs (ORF2 and ORF3) that code for products of unknown function. Sequence comparisons showed that FgV5 has the highest aa sequence identities to Fusarium graminearum virus 4 (FgV4) (83.01% for ORF1, 78.70% for ORF2, and 76.27% for ORF3), suggesting that FgV5 and FgV4 should be regarded as members of different species. Phylogenetic analysis indicated that FgV5 belongs to a taxonomically unassigned dsRNA mycovirus group that is related to the families Amalgaviridae and Partitiviridae. Here, we propose that FgV5 and related viruses are members of a yet to be named and formally recognized new family.

ACS Style

Luan Wang; Shuangchao Wang; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo. The complete genome sequence of a double-stranded RNA mycovirus from Fusarium graminearum strain HN1. Archives of Virology 2017, 162, 2119 -2124.

AMA Style

Luan Wang, Shuangchao Wang, Xiufen Yang, Hongmei Zeng, Dewen Qiu, Lihua Guo. The complete genome sequence of a double-stranded RNA mycovirus from Fusarium graminearum strain HN1. Archives of Virology. 2017; 162 (7):2119-2124.

Chicago/Turabian Style

Luan Wang; Shuangchao Wang; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo. 2017. "The complete genome sequence of a double-stranded RNA mycovirus from Fusarium graminearum strain HN1." Archives of Virology 162, no. 7: 2119-2124.

Journal article
Published: 02 March 2017 in Genome Announcements
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We describe a novel positive single-stranded RNA virus, termed Fusarium langsethiae hypovirus 1 (FlHV1), from the isolate AH32 of the phytopathogenic fungus Fusarium langsethiae . The properties of FlHV1 permit assignment to the genus Alphahypovirus in the family Hypoviridae . This is the first report of a mycovirus identified in F. langsethiae .

ACS Style

Pengfei Li; Xiaoguang Chen; Hao He; Dewen Qiu; Lihua Guo. Complete Genome Sequence of a Novel Hypovirus from the Phytopathogenic Fungus Fusarium langsethiae. Genome Announcements 2017, 5, e01722-16 .

AMA Style

Pengfei Li, Xiaoguang Chen, Hao He, Dewen Qiu, Lihua Guo. Complete Genome Sequence of a Novel Hypovirus from the Phytopathogenic Fungus Fusarium langsethiae. Genome Announcements. 2017; 5 (9):e01722-16.

Chicago/Turabian Style

Pengfei Li; Xiaoguang Chen; Hao He; Dewen Qiu; Lihua Guo. 2017. "Complete Genome Sequence of a Novel Hypovirus from the Phytopathogenic Fungus Fusarium langsethiae." Genome Announcements 5, no. 9: e01722-16.

Journal article
Published: 17 November 2016 in International Journal of Molecular Sciences
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Fusarium graminearum hypovirus 1 (FgHV1), which is phylogenetically related to Cryphonectria hypovirus 1 (CHV1), is a virus in the family Hypoviridae that infects the plant pathogenic fungus F. graminearum. Although hypovirus FgHV1 infection does not attenuate the virulence of the host (hypovirulence), it results in defects in mycelial growth and spore production. We now report that the vertical transmission rate of FgHV1 through asexual spores reached 100%. Using RNA deep sequencing, we performed genome-wide expression analysis to reveal phenotype-related genes with expression changes in response to FgHV1 infection. A total of 378 genes were differentially expressed, suggesting that hypovirus infection causes a significant alteration of fungal gene expression. Nearly two times as many genes were up-regulated as were down-regulated. A differentially expressed gene enrichment analysis identified a number of important pathways. Metabolic processes, the ubiquitination system, and especially cellular redox regulation were the most affected categories in F. graminearum challenged with FgHV1. The p20, encoded by FgHV1 could induce H2O2 accumulation and hypersensitive response in Nicotiana benthamiana leaves. Moreover, hypovirus FgHV1 may regulate transcription factors and trigger the RNA silencing pathway in F. graminearum.

ACS Style

Shuangchao Wang; Jingze Zhang; Pengfei Li; Dewen Qiu; Lihua Guo. Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection. International Journal of Molecular Sciences 2016, 17, 1922 .

AMA Style

Shuangchao Wang, Jingze Zhang, Pengfei Li, Dewen Qiu, Lihua Guo. Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection. International Journal of Molecular Sciences. 2016; 17 (11):1922.

Chicago/Turabian Style

Shuangchao Wang; Jingze Zhang; Pengfei Li; Dewen Qiu; Lihua Guo. 2016. "Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection." International Journal of Molecular Sciences 17, no. 11: 1922.

Journal article
Published: 04 July 2016 in Archives of Virology
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The complete nucleotide sequence of Fusarium graminearum deltaflexivirus 1 (FgDFV1), a novel positive single-stranded (+ss) RNA mycovirus, was sequenced and analyzed. The complete genome of FgDFV1/BJ59 was shown to be 8246 nucleotides (nt) long excluding the poly(A) tail. FgDFV1/BJ59 was predicted to contain a large open reading frame (ORF 1) and four smaller ORFs (2-5). ORF1 encodes a putative replication-associated polyprotein (RP) of 2042 amino acids (aa) and contains three conserved domains, viral RNA methyltransferase (Mtr), viral RNA helicase (Hel) and RNA-dependent RNA polymerase (RdRp). ORFs 2-5 encode four putative small hypothetical proteins (12-18 kDa) with unknown biological functions. Phylogenetic analysis based on RP sequences indicated that FgDFV1 is phylogenetically related to soybean leaf-associated mycoflexivirus 1 (SlaMyfV1) and Sclerotinia sclerotiorum deltaflexivirus 1 (SsDFV1), which form a well-supported and independent group belonging to a newly proposed family Deltaflexiviridae within the order Tymovirales. However, FgDFV1 is markedly different from SsDFV1 and SlaMyfV1 in genome organization and nucleotide sequence. FgDFV1 may represent an additional species in the new genus Deltaflexivirus or possibly a new genus in the proposed family Deltaflexiviridae.

ACS Style

Xiaoguang Chen; Hao He; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo. The complete genome sequence of a novel Fusarium graminearum RNA virus in a new proposed family within the order Tymovirales. Archives of Virology 2016, 161, 2899 -2903.

AMA Style

Xiaoguang Chen, Hao He, Xiufen Yang, Hongmei Zeng, Dewen Qiu, Lihua Guo. The complete genome sequence of a novel Fusarium graminearum RNA virus in a new proposed family within the order Tymovirales. Archives of Virology. 2016; 161 (10):2899-2903.

Chicago/Turabian Style

Xiaoguang Chen; Hao He; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo. 2016. "The complete genome sequence of a novel Fusarium graminearum RNA virus in a new proposed family within the order Tymovirales." Archives of Virology 161, no. 10: 2899-2903.

Journal article
Published: 18 May 2016 in Scientific Reports
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Previously, we characterized F. graminearum hypovirus 1 (FgHV1) and F. graminearum hypovirus 2 (FgHV2), which are the only two hypoviruses in F. graminearum that are closely related to Cryphonectria hypovirus 1 (CHV1) and Cryphonectria hypovirus 2 (CHV2) in the Hypoviridae family. In this study, we preliminarily elucidated the RNA silencing mechanism of the F. graminearum/hypovirus system from a small RNA (sRNA) perspective by using HiSeq deep sequencing. The length distributions of F. graminearum sRNA were altered by hypoviral infection. Potential microRNA-like (milRNA) candidates were differentially expressed between the hypovirus-free and hypovirus-infected library types. Extensive virus-derived small interfering RNAs (vsiRNAs) were also principally defined. The 1,831,081 and 3,254,758 total reads generated from the FgHV1 and FgHV2 genomes in F. graminearum yielded the first high-resolution sRNA maps of fungal viruses. In addition, extensive bioinformatics searches identified a large number of transcripts that are potentially targeted by vsiRNAs, several of which were effectively down-regulated. In particular, the RNA silencing-related genes FgDicer1 and FgRdRp5 were predicted targets of FgHV1- and FgHV2-derived siRNAs, possibly revealing a novel anti-RNA silencing strategy employed by mycoviruses.

ACS Style

Shuangchao Wang; Pengfei Li; Jingze Zhang; Dewen Qiu; Lihua Guo. Generation of a high resolution map of sRNAs from Fusarium graminearum and analysis of responses to viral infection. Scientific Reports 2016, 6, 26151 .

AMA Style

Shuangchao Wang, Pengfei Li, Jingze Zhang, Dewen Qiu, Lihua Guo. Generation of a high resolution map of sRNAs from Fusarium graminearum and analysis of responses to viral infection. Scientific Reports. 2016; 6 (1):26151.

Chicago/Turabian Style

Shuangchao Wang; Pengfei Li; Jingze Zhang; Dewen Qiu; Lihua Guo. 2016. "Generation of a high resolution map of sRNAs from Fusarium graminearum and analysis of responses to viral infection." Scientific Reports 6, no. 1: 26151.

Journal article
Published: 01 February 2016 in Virology
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We isolated a novel mycovirus, Fusarium graminearum mycotymovirus 1 (FgMTV1/SX64), which is related to members of the family Tymoviridae, from the plant pathogenic fungus F. graminearum strain SX64. The complete 7863 nucleotide sequence of FgMTV1/SX64, excluding the poly (A) tail, was determined. The genome of FgMTV1/SX64 is predicted to contain four open reading frames (ORFs). The largest ORF1 is 6723 nucleotides (nt) in length and encodes a putative polyprotein of 2242 amino acids (aa), which contains four conserved domains, a methyltransferase (Mtr), tymovirus endopeptidase (Pro), viral RNA helicase (Hel), and RNA-dependent RNA polymerase (RdRp), of the replication-associated proteins (RPs) of the positive-strand RNA viruses. ORFs 2-4 putatively encode three putative small hypothetical proteins, but their functions are still unknown. Sequence alignments and phylogenetic analyses based on the putative RP protein and the three conserved domains (Mtr, Hel and RdRp) showed that FgMTV1/SX64 is most closely related to, but distinctly branched from, the viruses from the family Tymoviridae. Although FgMTV1/SX64 infection caused mild or no effect on conidia production, biomass and virulence of its host F. graminearum strain SX64, its infection had significant effects on the growth rate, colony diameter and deoxynivalenol (DON) production. This is the first molecular characterization of a tymo-like mycovirus isolated from a plant pathogenic fungus. It is proposed that the mycovirus FgMTV1/SX64 is a representative member of new proposed lineage Mycotymovirus in the family Tymoviridae.

ACS Style

Pengfei Li; Yanhong Lin; Hailong Zhang; Shuangchao Wang; Dewen Qiu; Lihua Guo. Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum. Virology 2016, 489, 86 -94.

AMA Style

Pengfei Li, Yanhong Lin, Hailong Zhang, Shuangchao Wang, Dewen Qiu, Lihua Guo. Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum. Virology. 2016; 489 ():86-94.

Chicago/Turabian Style

Pengfei Li; Yanhong Lin; Hailong Zhang; Shuangchao Wang; Dewen Qiu; Lihua Guo. 2016. "Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum." Virology 489, no. : 86-94.

Journal article
Published: 01 July 2015 in Virology
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A novel mycovirus, termed Fusarium graminearum Hypovirus 2 (FgHV2/JS16), isolated from a plant pathogenic fungus, Fusarium graminearum strain JS16, was molecularly and biologically characterized. The genome of FgHV2/JS16 is 12,800 nucleotides (nts) long, excluding the poly (A) tail. This genome has only one large putative open reading frame, which encodes a polyprotein containing three normal functional domains, papain-like protease, RNA-dependent RNA polymerase, RNA helicase, and a novel domain with homologous bacterial SMC (structural maintenance of chromosomes) chromosome segregation proteins. A defective RNA segment that is 4553-nts long, excluding the poly (A) tail, was also detected in strain JS16. The polyprotein shared significant aa identities with Cryphonectria hypovirus 1 (CHV1) (16.8%) and CHV2 (16.2%). Phylogenetic analyses based on multiple alignments of the polyprotein clearly divided the members of Hypoviridae into two major groups, suggesting that FgHV2/JS16 was a novel hypovirus of a newly proposed genus-Alphahypovirus-composed of the members of Group 1, including CHV1, CHV2, FgHV1 and Sclerotinia sclerotiorum hypovirus 2. FgHV2/JS16 was shown to be associated with hypovirulence phenotypes according to comparisons of the biological properties shared between FgHV2/JS16-infected and FgHV2/JS16-free isogenic strains. Furthermore, we demonstrated that FgHV2/JS16 infection activated the RNA interference pathway in Fusarium graminearum by relative quantitative real time RT-PCR.

ACS Style

Pengfei Li; Hailong Zhang; Xiaoguang Chen; Dewen Qiu; Lihua Guo. Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum. Virology 2015, 481, 151 -160.

AMA Style

Pengfei Li, Hailong Zhang, Xiaoguang Chen, Dewen Qiu, Lihua Guo. Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum. Virology. 2015; 481 ():151-160.

Chicago/Turabian Style

Pengfei Li; Hailong Zhang; Xiaoguang Chen; Dewen Qiu; Lihua Guo. 2015. "Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum." Virology 481, no. : 151-160.

Journal article
Published: 01 February 2015 in Biochemical and Biophysical Research Communications
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In this study, a necrosis-inducing protein was purified from the culture filtrate of the necrotrophic fungus Botrytis cinerea BC-98 strain. Secreted proteins were collected and fractionated by liquid chromatography. The fraction with the highest necrosis-inducing activity was further purified. A glycoprotein named BcGs1 was identified by 2D electrophoresis and mass spectrometry. The BcGs1 protein consisted of 672 amino acids with a theoretical molecular weight of 70.487 kDa. Functional domain analysis indicated that BcGs1 was a glucan 1,4-alpha-glucosidase, a cell wall-degrading enzyme, with a Glyco_hydro_15 domain and a CBM20_glucoamylase domain. The BcGs1 protein caused necrotic lesions that mimicked a typical hypersensitive response and H2O2 production in tomato and tobacco leaves. BcGs1-treated plants exhibited resistance to B. cinerea, Pseudomonas syringae pv. tomato DC3000 and tobacco mosaic virus in systemic leaves. In addition, BcGs1 triggered elevation of the transcript levels of the defence-related genes PR-1a, TPK1b and Prosystemin. This is the first report of a Botrytis glucan 1,4-alpha-glucosidase triggering host plant immunity as an elicitor. These results lay a foundation for further study of the comprehensive interaction between plants and necrotrophic fungi.

ACS Style

Yi Zhang; Yunhua Zhang; Dewen Qiu; Hongmei Zeng; Lihua Guo; Xiufen Yang. BcGs1, a glycoprotein from Botrytis cinerea , elicits defence response and improves disease resistance in host plants. Biochemical and Biophysical Research Communications 2015, 457, 627 -634.

AMA Style

Yi Zhang, Yunhua Zhang, Dewen Qiu, Hongmei Zeng, Lihua Guo, Xiufen Yang. BcGs1, a glycoprotein from Botrytis cinerea , elicits defence response and improves disease resistance in host plants. Biochemical and Biophysical Research Communications. 2015; 457 (4):627-634.

Chicago/Turabian Style

Yi Zhang; Yunhua Zhang; Dewen Qiu; Hongmei Zeng; Lihua Guo; Xiufen Yang. 2015. "BcGs1, a glycoprotein from Botrytis cinerea , elicits defence response and improves disease resistance in host plants." Biochemical and Biophysical Research Communications 457, no. 4: 627-634.

Journal article
Published: 24 September 2014 in Archives of Virology
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The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3′ poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5′-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3′-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.

ACS Style

Yanhong Lin; Hailong Zhang; Chengjin Zhao; Shengxue Liu; Lihua Guo. The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Archives of Virology 2014, 160, 577 -580.

AMA Style

Yanhong Lin, Hailong Zhang, Chengjin Zhao, Shengxue Liu, Lihua Guo. The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Archives of Virology. 2014; 160 (2):577-580.

Chicago/Turabian Style

Yanhong Lin; Hailong Zhang; Chengjin Zhao; Shengxue Liu; Lihua Guo. 2014. "The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28." Archives of Virology 160, no. 2: 577-580.

Journal article
Published: 01 May 2014 in Microbiological Research
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In our previous study, PevD1 was characterized as a novel protein elicitor produced by Verticillium dahliae inducing hypersensitive response (HR) and systemic acquired resistance (SAR) in tobacco plants; however, the detailed mechanisms of PevD1's elicitor activity remain unclear. In this study, five mutant fragments of PevD1 were generated by polymerase chain reaction-based mutagenesis and the truncated proteins expressed in Escherichia coli were used to test their elicitor activities. Biological activity analysis showed that the N-terminal and C-terminal of PevD1 had distinct influence on HR and SAR elicitation. Fragment PevD1ΔN98, which spans the C-terminal 57 amino acids of PevD1, was critical for the induction of HR in tobacco plants. In contrast, fragment PevD1ΔC57, the N-terminal of 98 amino acids of PevD1, retained the ability to induce SAR against tobacco mosaic virus (TMV) but not induction of HR, suggesting that the induction of HR is not essential for SAR mediated by PevD1. Our results indicated that fragment PevD1ΔC57 could be a candidate peptide for plant protection against pathogens without causing negative effects.

ACS Style

Wenxian Liu; Hongmei Zeng; Zhipeng Liu; Xiufen Yang; Lihua Guo; Dewen Qiu. Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco. Microbiological Research 2014, 169, 476 -482.

AMA Style

Wenxian Liu, Hongmei Zeng, Zhipeng Liu, Xiufen Yang, Lihua Guo, Dewen Qiu. Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco. Microbiological Research. 2014; 169 (5):476-482.

Chicago/Turabian Style

Wenxian Liu; Hongmei Zeng; Zhipeng Liu; Xiufen Yang; Lihua Guo; Dewen Qiu. 2014. "Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco." Microbiological Research 169, no. 5: 476-482.

Journal article
Published: 01 January 2013 in Acta Agronomica Sinica
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Xue-Cong Peng; Xiu-Fen Yang; De-Wen Qiu; Hong-Mei Zeng; Li-Hua Guo; Zheng Liu. Overexpression of Elicitor Protein Encoding Genehrip1Improves Tolerance to Drought and Salt Stresses inArabidopsis. Acta Agronomica Sinica 2013, 39, 1 .

AMA Style

Xue-Cong Peng, Xiu-Fen Yang, De-Wen Qiu, Hong-Mei Zeng, Li-Hua Guo, Zheng Liu. Overexpression of Elicitor Protein Encoding Genehrip1Improves Tolerance to Drought and Salt Stresses inArabidopsis. Acta Agronomica Sinica. 2013; 39 (8):1.

Chicago/Turabian Style

Xue-Cong Peng; Xiu-Fen Yang; De-Wen Qiu; Hong-Mei Zeng; Li-Hua Guo; Zheng Liu. 2013. "Overexpression of Elicitor Protein Encoding Genehrip1Improves Tolerance to Drought and Salt Stresses inArabidopsis." Acta Agronomica Sinica 39, no. 8: 1.

Journal article
Published: 01 January 2012 in Molecular Plant Breeding
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Sehroon Khan; Lihua Guo; Yushanjiang Maimaiti; Mahmut Mijit; Dewen Qiu. Entomopathogenic Fungi as Microbial Biocontrol Agent. Molecular Plant Breeding 2012, 3, 1 .

AMA Style

Sehroon Khan, Lihua Guo, Yushanjiang Maimaiti, Mahmut Mijit, Dewen Qiu. Entomopathogenic Fungi as Microbial Biocontrol Agent. Molecular Plant Breeding. 2012; 3 (1):1.

Chicago/Turabian Style

Sehroon Khan; Lihua Guo; Yushanjiang Maimaiti; Mahmut Mijit; Dewen Qiu. 2012. "Entomopathogenic Fungi as Microbial Biocontrol Agent." Molecular Plant Breeding 3, no. 1: 1.

English abstract
Published: 01 September 2011 in Sheng Wu Gong Cheng Xue Bao
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Limei Wang; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo; Zheng Liu. [Extracellular expression of protein elicitor PeaT1 in Bacillus subtilis to enhance drought tolerance and growth in wheat]. Sheng Wu Gong Cheng Xue Bao 2011, 27, 1 .

AMA Style

Limei Wang, Xiufen Yang, Hongmei Zeng, Dewen Qiu, Lihua Guo, Zheng Liu. [Extracellular expression of protein elicitor PeaT1 in Bacillus subtilis to enhance drought tolerance and growth in wheat]. Sheng Wu Gong Cheng Xue Bao. 2011; 27 (9):1.

Chicago/Turabian Style

Limei Wang; Xiufen Yang; Hongmei Zeng; Dewen Qiu; Lihua Guo; Zheng Liu. 2011. "[Extracellular expression of protein elicitor PeaT1 in Bacillus subtilis to enhance drought tolerance and growth in wheat]." Sheng Wu Gong Cheng Xue Bao 27, no. 9: 1.