Mussels (Mytilus galloprovincialis) collected at three locations in Boka Kotorska Bay, on the Montenegrin Adriatic coast, were analyzed for the first time by optical and Raman microscopy to detect microplastics (MPs) and other emerging contaminants in their soft tissues. Concentrations of six trace metals (Cu, Zn, Mn, Fe, Cd, and Hg) were also measured in the same samples by atomic absorption spectroscopy. Mussels from a location near the urban area of Kotor were found to exhibit the highest content of MPs and other pollutants originating from anthropogenic sources, while farmed mussels showed higher carotenoid as well as nylon content. The hypothesis of MPs acting as a possible secondary route of trace metals ingress in mussels, a thus far scarcely studied topic, was evaluated based on a comparative analysis of the obtained results. In this context, it was noticed that nylon filaments originating from mussel farming equipment might contribute to higher trace metal content. The results showed that the simultaneous analysis of different contaminants in mussels can be a significant step forward in marine environment pollution monitoring and the assessment of human health risks associated with the consumption of contaminated seafood.

Considering that microplastics are widespread in the marine environment, in this study we evaluated the presence, identify distribution, abundance, shape type, and color of microplastics in surface sediment along the Montenegrin coast, on the Adriatic Sea. These preliminary results provide the first published record of microplastics found in the surface sediment of this area and highlight the importance of microplastics as a component of marine debris. We documented the presence of microplastics at all sampling locations. The identification of polymer types was performed using Fourier-transform infrared (FTIR) spectroscopy, whereby the presence of three polymer types became evident: polypropylene (54.5%), polyethylene (9.7%), and acrylate copolymer (2.0%). Another 22.2% of particles were unidentified polymers, and the remaining 11.5% were non-synthetic materials. The most common shape type of microplastics was filaments (55.5%), followed by granules (26.3%), fragments (14.9%), and films (3.3%). The dominant colors of microplastics followed the order: blue > yellow > red > clear > black > green > blue-white > white. The average abundance of microplastics in all sampling locations was 609 pieces of microplastic/kg of dry sediment. Compared with other studies, the surface sediment of the Montenegrin coast is moderately to highly polluted with microplastics, depending on the examined location.