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We explored the relationship between cultural and social participation, physical activity, and well-being in a group of residents of the metropolitan area of Naples, Italy and the role that resilience plays in this relationship. Naples offers a remarkable urban environment with the potentially beneficial psychological effects of outstanding natural beauty, and one of the world’s most impressive repositories of tangible and intangible cultural heritage. However, Naples was also, and still is, heavily affected by the 2008 economic crisis, in addition to preexisting social and economic issues. The major finding of this study is that, despite this highly contrasting urban environment, the combination of physical activity and engagement in social and cultural activities has a positive effect on subjective (self-reported) psychological well-being (SPWB) in a group of residents, and that resilience mediates this relationship.
Sergio Cocozza; Pier Luigi Sacco; Giuseppe Matarese; Gayle D. Maffulli; Nicola Maffulli; Donatella Tramontano. Participation to Leisure Activities and Well-Being in a Group of Residents of Naples-Italy: The Role of Resilience. International Journal of Environmental Research and Public Health 2020, 17, 1895 .
AMA StyleSergio Cocozza, Pier Luigi Sacco, Giuseppe Matarese, Gayle D. Maffulli, Nicola Maffulli, Donatella Tramontano. Participation to Leisure Activities and Well-Being in a Group of Residents of Naples-Italy: The Role of Resilience. International Journal of Environmental Research and Public Health. 2020; 17 (6):1895.
Chicago/Turabian StyleSergio Cocozza; Pier Luigi Sacco; Giuseppe Matarese; Gayle D. Maffulli; Nicola Maffulli; Donatella Tramontano. 2020. "Participation to Leisure Activities and Well-Being in a Group of Residents of Naples-Italy: The Role of Resilience." International Journal of Environmental Research and Public Health 17, no. 6: 1895.
Acute administration of a high level of extracellular citrate displays an anti-proliferative effect on both in vitro and in vivo models. However, the long-term effect of citrate treatment has not been investigated yet. Here, we address this question in PC3 cells, a prostate-cancer-derived cell line. Acute administration of high levels of extracellular citrate impaired cell adhesion and inhibited the proliferation of PC3 cells, but surviving cells adapted to grow in the chronic presence of 20 mM citrate. Citrate-resistant PC3 cells are significantly less glycolytic than control cells. Moreover, they overexpress short-form, citrate-insensitive phosphofructokinase 1 (PFK1) together with full-length PFK1. In addition, they show traits of mesenchymal-epithelial transition: an increase in E-cadherin and a decrease in vimentin. In comparison with PC3 cells, citrate-resistant cells display morphological changes that involve both microtubule and microfilament organization. This was accompanied by changes in homeostasis and the organization of intracellular organelles. Thus, the mitochondrial network appears fragmented, the Golgi complex is scattered, and the lysosomal compartment is enlarged. Interestingly, citrate-resistant cells produce less total ROS but accumulate more mitochondrial ROS than control cells. Consistently, in citrate-resistant cells, the autophagic pathway is upregulated, possibly sustaining their survival. In conclusion, chronic administration of citrate might select resistant cells, which could jeopardize the benefits of citrate anticancer treatment.
Carmen Caiazza; Massimo D’Agostino; Fabiana Passaro; Deriggio Faicchia; Massimo Mallardo; Simona Paladino; Giovanna Maria Pierantoni; Donatella Tramontano. Effects of Long-Term Citrate Treatment in the PC3 Prostate Cancer Cell Line. International Journal of Molecular Sciences 2019, 20, 2613 .
AMA StyleCarmen Caiazza, Massimo D’Agostino, Fabiana Passaro, Deriggio Faicchia, Massimo Mallardo, Simona Paladino, Giovanna Maria Pierantoni, Donatella Tramontano. Effects of Long-Term Citrate Treatment in the PC3 Prostate Cancer Cell Line. International Journal of Molecular Sciences. 2019; 20 (11):2613.
Chicago/Turabian StyleCarmen Caiazza; Massimo D’Agostino; Fabiana Passaro; Deriggio Faicchia; Massimo Mallardo; Simona Paladino; Giovanna Maria Pierantoni; Donatella Tramontano. 2019. "Effects of Long-Term Citrate Treatment in the PC3 Prostate Cancer Cell Line." International Journal of Molecular Sciences 20, no. 11: 2613.
Natural selection acts on genetic variants by increasing the frequency of alleles responsible for a cellular function that is favorable in a certain environment. In a previous genome-wide scan for positive selection in contemporary humans, we identified a signal of positive selection in European and Asians at the genetic variant rs10180970. The variant is located in the second intron of the ABCA12 gene, which is implicated in the lipid barrier formation and down-regulated by UVB radiation. We studied the signal of selection in the genomic region surrounding rs10180970 in a larger dataset that includes DNA sequences from ancient samples. We also investigated the functional consequences of gene expression of the alleles of rs10180970 and another genetic variant in its proximity in healthy volunteers exposed to similar UV radiation. We confirmed the selection signal and refine its location that extends over 35 kb and includes the first intron, the first two exons and the transcription starting site of ABCA12. We found no obvious effect of rs10180970 alleles on ABCA12 gene expression. We reconstructed the trajectory of the T allele over the last 80,000 years to discover that it was specific to H. sapiens and present in non-Africans 45,000 years ago.
Roberto Sirica; Marianna Buonaiuto; Valeria Petrella; Lucia Sticco; Donatella Tramontano; Dario Antonini; Caterina Missero; Ombretta Guardiola; Gennaro Andolfi; Heerman Kumar; Qasim Ayub; Yali Xue; Chris Tyler-Smith; Marco Salvemini; Giovanni D’Angelo; Vincenza Colonna. Positive selection in Europeans and East-Asians at the ABCA12 gene. Scientific Reports 2019, 9, 4843 .
AMA StyleRoberto Sirica, Marianna Buonaiuto, Valeria Petrella, Lucia Sticco, Donatella Tramontano, Dario Antonini, Caterina Missero, Ombretta Guardiola, Gennaro Andolfi, Heerman Kumar, Qasim Ayub, Yali Xue, Chris Tyler-Smith, Marco Salvemini, Giovanni D’Angelo, Vincenza Colonna. Positive selection in Europeans and East-Asians at the ABCA12 gene. Scientific Reports. 2019; 9 (1):4843.
Chicago/Turabian StyleRoberto Sirica; Marianna Buonaiuto; Valeria Petrella; Lucia Sticco; Donatella Tramontano; Dario Antonini; Caterina Missero; Ombretta Guardiola; Gennaro Andolfi; Heerman Kumar; Qasim Ayub; Yali Xue; Chris Tyler-Smith; Marco Salvemini; Giovanni D’Angelo; Vincenza Colonna. 2019. "Positive selection in Europeans and East-Asians at the ABCA12 gene." Scientific Reports 9, no. 1: 4843.
In February 2017, the "Programma Mattone Internazionale Salute" (ProMis), that is the Italian Program for Internationalization of Regional Health Systems of the Ministry of Health (MoH), presented the first version of its Position Paper on Health Tourism, which embeds a first shared approach to the recommendations expressed by the European Committee of Regions (CoR) on "Age-Friendly" tourism. The CoR stresses the importance of local and regional authorities in the coordination of multi-sectoral policies such as healthcare, social assistance, transport, urban planning and rural development in relation to the promotion of mobility, security, accessibility of services, including health care and social services. "Age-friendly" tourism is an example of an innovative tourist offer that strives to meet the health needs of the entire "traveling" population, with an integrated and cross-sector approach that involves various organizations operating in sectors such as healthcare, accessibility and transport. The aim of the workshop was to explore the interest of the stakeholders to participate in a systemic action in the field of "health" tourism, and to identify priority implementation areas that offer opportunities to take advantage of validated, innovative experiences that strengthen the accessibility to health and social services in regional, national and international contexts. This effort provides the opportunity to take advantage of aligning the European Structural and Investment Funds (ESIF) to the development of tourism, coherently with the needs and resources of local and regional health authorities.
M Illario; V De Luca; L Leonardini; M Kucharczyk; A S Parent; C Dantas; A L Jegundo; W Van Staalduinen; J Ganzarain; L Comisso; C Bramezza; A M Carriazo; A Maritati; G Tramontano; P Capozzi; E Goossens; C Cotrone; A Costantini; M Ciliberti; M Femiano; A D’Amore; M Forlenza; R Ruggiero; A Bianchi; L Augustin; V Marrazzo; T Dello Ioio; S Capaldo; A Crudeli; G De Cesare; F Cuccaro; G Bracale; Donatella Tramontano; A Postiglione; C Matera; E Coscioni; J Bousquet. Health tourism: an opportunity for sustainable development. 2019, 19, 109 -115.
AMA StyleM Illario, V De Luca, L Leonardini, M Kucharczyk, A S Parent, C Dantas, A L Jegundo, W Van Staalduinen, J Ganzarain, L Comisso, C Bramezza, A M Carriazo, A Maritati, G Tramontano, P Capozzi, E Goossens, C Cotrone, A Costantini, M Ciliberti, M Femiano, A D’Amore, M Forlenza, R Ruggiero, A Bianchi, L Augustin, V Marrazzo, T Dello Ioio, S Capaldo, A Crudeli, G De Cesare, F Cuccaro, G Bracale, Donatella Tramontano, A Postiglione, C Matera, E Coscioni, J Bousquet. Health tourism: an opportunity for sustainable development. . 2019; 19 ():109-115.
Chicago/Turabian StyleM Illario; V De Luca; L Leonardini; M Kucharczyk; A S Parent; C Dantas; A L Jegundo; W Van Staalduinen; J Ganzarain; L Comisso; C Bramezza; A M Carriazo; A Maritati; G Tramontano; P Capozzi; E Goossens; C Cotrone; A Costantini; M Ciliberti; M Femiano; A D’Amore; M Forlenza; R Ruggiero; A Bianchi; L Augustin; V Marrazzo; T Dello Ioio; S Capaldo; A Crudeli; G De Cesare; F Cuccaro; G Bracale; Donatella Tramontano; A Postiglione; C Matera; E Coscioni; J Bousquet. 2019. "Health tourism: an opportunity for sustainable development." 19, no. : 109-115.
Natural selection acts on genetic variants by increasing the frequency of alleles responsible for a cellular function that is favorable in a certain environment. In a previous genome-wide scan for positive selection in contemporary humans, we identified a signal of positive selection in European and Asians at the genetic variant rs10180970. The variant is located in the second intron of the ABCA12 gene, which is implicated in the lipid barrier formation and down-regulated by UVB radiation. We studied the signal of selection in the genomic region surrounding rs10180970 in a larger dataset that includes DNA sequences from ancient samples. We also investigated the functional consequences of gene expression of the alleles of rs10180970 and another genetic variant in its proximity in healthy volunteers exposed to similar UV radiation.We confirmed the selection signal and refine its location that extends over 35 kb and includes the first intron, the first two exons and the transcription starting site of ABCA12. We found no obvious effect of rs10180970 alleles on ABCA12 gene expression. We reconstructed the trajectory of the T allele over the last 80,000 years to discover that it was specific to H. sapiens and frequent among non-Africans already 45,000 years ago.
Roberto Sirica; Marianna Buonaiuto; Valeria Petrella; Lucia Sticco; Donatella Tramontano; Dario Antonini; Caterina Missero; Ombretta Guardiola; Gennaro Andolfi; Heerman Kumar; Qasim Ayub; Yali Xue; Chris Tyler-Smith; Marco Salvemini; Giovanni D'angelo; Vincenza Colonna. Positive selection in Europeans and East-Asians at the ABCA12 gene. 2018, 392811 .
AMA StyleRoberto Sirica, Marianna Buonaiuto, Valeria Petrella, Lucia Sticco, Donatella Tramontano, Dario Antonini, Caterina Missero, Ombretta Guardiola, Gennaro Andolfi, Heerman Kumar, Qasim Ayub, Yali Xue, Chris Tyler-Smith, Marco Salvemini, Giovanni D'angelo, Vincenza Colonna. Positive selection in Europeans and East-Asians at the ABCA12 gene. . 2018; ():392811.
Chicago/Turabian StyleRoberto Sirica; Marianna Buonaiuto; Valeria Petrella; Lucia Sticco; Donatella Tramontano; Dario Antonini; Caterina Missero; Ombretta Guardiola; Gennaro Andolfi; Heerman Kumar; Qasim Ayub; Yali Xue; Chris Tyler-Smith; Marco Salvemini; Giovanni D'angelo; Vincenza Colonna. 2018. "Positive selection in Europeans and East-Asians at the ABCA12 gene." , no. : 392811.
A successful aging could be gained by life satisfaction, social functioning, or psychological resources and, definitely, by increasing resistance to diverse age‐related pathologies. Nowadays, cancer can be considered an age‐related disease since the incidence of most cancers increases with age, rising more rapidly beginning in midlife. Although adults with extended longevity are less likely to develop cancer, it is now emerging that aging and cancer share common molecular links, and thus targeting these mechanisms may be suitable to treat multiple disorders, for the prolonging of healthy aging. At present, one of the cornerstones of antiaging is hormone‐replacement therapy to treat diseases associated with a state of age‐related sex‐hormone deficiency in women and men; however, many studies question the relationship of hormone replacement to cancer recurrence. Here, we discuss signaling and metabolic molecular crossroad linking aging and cancer. This is useful to argue about the current knowledge of prolongevity and druggable targets and to motivate specific intervention strategies that could modify practices of the aging population, activating multiple longevity pathways but keeping track of cancer pathways, thereby potentially preserving health status.
Donatella Tramontano; Francesca De Amicis. Is the secret for a successful aging to keep track of cancer pathways? Journal of Cellular Physiology 2018, 233, 8467 -8476.
AMA StyleDonatella Tramontano, Francesca De Amicis. Is the secret for a successful aging to keep track of cancer pathways? Journal of Cellular Physiology. 2018; 233 (11):8467-8476.
Chicago/Turabian StyleDonatella Tramontano; Francesca De Amicis. 2018. "Is the secret for a successful aging to keep track of cancer pathways?" Journal of Cellular Physiology 233, no. 11: 8467-8476.
High Mobility Group A1 (HMGA1) is an architectural chromatin protein whose overexpression is a feature of malignant neoplasias with a causal role in cancer initiation and progression. HMGA1 promotes tumor growth by several mechanisms, including increase of cell proliferation and survival, impairment of DNA repair and induction of chromosome instability. Autophagy is a self-degradative process that, by providing energy sources and removing damaged organelles and misfolded proteins, allows cell survival under stress conditions. On the other hand, hyper-activated autophagy can lead to non-apoptotic programmed cell death. Autophagy deregulation is a common feature of cancer cells in which has a complex role, showing either an oncogenic or tumor suppressor activity, depending on cellular context and tumor stage. Here, we report that depletion of HMGA1 perturbs autophagy by different mechanisms. HMGA1-knockdown increases autophagosome formation by constraining the activity of the mTOR pathway, a major regulator of autophagy, and transcriptionally upregulating the autophagy-initiating kinase Unc-51-like kinase 1 (ULK1). Consistently, functional experiments demonstrate that HMGA1 binds ULK1 promoter region and negatively regulates its transcription. On the other hand, the increase in autophagosomes is not associated to a proportionate increase in their maturation. Overall, the effects of HMGA1 depletion on autophagy are associated to a decrease in cell proliferation and ultimately impact on cancer cells viability. Importantly, silencing of ULK1 prevents the effects of HMGA1-knockdown on cellular proliferation, viability and autophagic activity, highlighting how these effects are, at least in part, mediated by ULK1. Interestingly, this phenomenon is not restricted to skin cancer cells, as similar results have been observed also in HeLa cells silenced for HMGA1. Taken together, these results clearly indicate HMGA1 as a key regulator of the autophagic pathway in cancer cells, thus suggesting a novel mechanism through which HMGA1 can contribute to cancer progression.
Andrea Conte; Simona Paladino; Gaia Bianco; Dominga Fasano; Raffaele Gerlini; Mara Tornincasa; Maurizio Renna; Alfredo Fusco; Donatella Tramontano; Giovanna Maria Pierantoni. High mobility group A1 protein modulates autophagy in cancer cells. Cell Death & Differentiation 2017, 24, 1948 -1962.
AMA StyleAndrea Conte, Simona Paladino, Gaia Bianco, Dominga Fasano, Raffaele Gerlini, Mara Tornincasa, Maurizio Renna, Alfredo Fusco, Donatella Tramontano, Giovanna Maria Pierantoni. High mobility group A1 protein modulates autophagy in cancer cells. Cell Death & Differentiation. 2017; 24 (11):1948-1962.
Chicago/Turabian StyleAndrea Conte; Simona Paladino; Gaia Bianco; Dominga Fasano; Raffaele Gerlini; Mara Tornincasa; Maurizio Renna; Alfredo Fusco; Donatella Tramontano; Giovanna Maria Pierantoni. 2017. "High mobility group A1 protein modulates autophagy in cancer cells." Cell Death & Differentiation 24, no. 11: 1948-1962.
The Strategic Implementation Plan of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) proposed six Action Groups. After almost three years of activity, many achievements have been obtained through commitments or collaborative work of the Action Groups. However, they have often worked in silos and, consequently, synergies between Action Groups have been proposed to strengthen the triple win of the EIP on AHA. The paper presents the methodology and current status of the Task Force on EIP on AHA synergies. Synergies are in line with the Action Groups’ new Renovated Action Plan (2016-2018) to ensure that their future objectives are coherent and fully connected. The outcomes and impact of synergies are using the Monitoring and Assessment Framework for the EIP on AHA (MAFEIP). Eight proposals for synergies have been approved by the Task Force: Five cross-cutting synergies which can be used for all current and future synergies as they consider overarching domains (appropriate polypharmacy, citizen empowerment, teaching and coaching on AHA, deployment of synergies to EU regions, Responsible Research and Innovation), and three cross-cutting synergies focussing on current Action Group activities (falls, frailty, integrated care and chronic respiratory diseases).
Jean Bousquet; M. Bewick; A. Cano; P. Eklund; Giuseppe Fico; N. Goswami; N. A. Guldemond; D. Henderson; M. J. Hinkema; G. Liotta; A. Mair; W. Molloy; A. Monaco; I. Monsonis-Paya; A. Nizinska; H. Papadopoulos; A. Pavlickova; S. Pecorelli; Alexandra Prados-Torres; R. E. Roller-Wirnsberger; D. Somekh; Cecilia Vera Muñoz; F. Visser; J. Farrell; João Malva; Karen Andersen-Ranberg; T. Camuzat; A. M. Carriazo; G. Crooks; Z. Gutter; Guido Iaccarino; E. Manuel De Keenoy; G. Moda; Leocadio Rodríguez-Mañas; T. Vontetsianos; C. Abreu; J. Alonso; C. Alonso-Bouzon; Joel Ankri; M. T. Arredondo; F. Avolio; A. Bedbrook; A. Z. Białoszewski; H. Blain; R. Bourret; M. F. Cabrera-Umpierrez; A. Catala; Rónán O'Caoimh; M. Cesari; Niels Chavannes; J. Correia-Da-Sousa; T. Dedeu; M. Ferrando; M. Ferri; W. J. Fokkens; F. Garcia-Lizana; O. Guérin; Peter Hellings; T. Haahtela; M. Illario; M. C. Inzerilli; K. C. Lodrup Carlsen; P. Kardas; T. Keil; M. Maggio; A. Mendez-Zorrilla; E. Menditto; J. Mercier; J. P. Michel; R. Murray; M. Nogues; I. O’Byrne-Maguire; D. Pappa; A. S. Parent; M. Pastorino; Carlos Manuel Da Silva Robalo Cordeiro; B. Samolinski; P. Siciliano; Ana Teixeira; S. I. Tsartara; A. Valiulis; O. Vandenplas; Tuula Vasankari; B. Vellas; M. Vollenbroek-Hutten; M. Wickman; Arzu Yorgancıoglu; Torsten Zuberbier; M. Barbagallo; Giorgio Walter Canonica; L. Klimek; S. Maggi; W. Aberer; C. Akdis; I. M. Adcock; I. Agache; C. Albera; Federico Alonso-Trujillo; M. Angel Guarcia; I. Annesi-Maesano; Joao Luis Alves Apostolo; S. H. Arshad; V. Attalin; A. Avignon; C. Bachert; I. Baroni; E. Bel; M. Benson; C. Bescos; F. Blasi; Cristina Bárbara; K. C. Bergmann; P. L. Bernard; Sergio Bonini; P. J. Bousquet; B. Branchini; Chris Brightling; V. Bruguière; C. Bunu; A. Bush; Davide Caimmi; M. A. Calderon; G. Canovas; Victoria Cardona; A. Cesario; E. Chkhartishvili; R. Chiron; T. Chivato; K. F. Chung; M. D’Angelantonio; G. De Carlo; D. Cholley; F. Chorin; B. Combe; B. Compas; D. J. Costa; Elisio Costa; O. Coste; A. -L. Coupet; G. Crepaldi; Adnan Custovic; R. Dahl; S. E. Dahlen; Pascal Demoly; P. Devillier; A. Didier; Anh Tuan Dinh-Xuan; Ratko Djukanovic; D. Dokic; George Du Toit; R. Dubakiene; A. Dupeyron; R. Emuzyte; Alessandro Fiocchi; A. Wagner; M. Fletcher; João Fonseca; B. Fougère; A. Gamkrelidze; G. Garces; J. Garcia-Aymeric; B. Garcia-Zapirain; B. Gemicioğlu; C. Gouder; B. Hellquist-Dahl; I. Hermosilla-Gimeno; D. Héve; Carol Holland; Marc Humbert; M. Hyland; Sebastian Johnston; J. Just; M. Jutel; Igor Kaidashev; M. Kaitov; O. Kalayci; A. F. Kalyoncu; W. Keijser; Huib Kerstjens; J. Knezović; M. Kowalski; Gerard Koppelman; T. Kotska; M. Kovac; I. Kull; Piotr Kuna; V. Kvedariene; V. Lepore; W. Macnee; A. Magnan; I. Majer; P. Manning; Maura Marcucci; T. Marti; M. Masoli; E. Melen; N. Miculinic; F. Mihaltan; B. Milenkovic; J. Millot-Keurinck; H. Mlinarić; I. Momas; S. Montefort; M. Morais-Almeida; Teresa Moreno-Casbas; R. Mösges; J. Mullol; Rachel Nadif; M. Nalin; Esperanza Navarro-Pardo; K. Nekam; Gregory Ninot; D. Paccard; Sandra Cristina Cozinheiro Fidalgo Rafael Gamboa Pais; E. Palummeri; Petr Panzner; N. K. Papadopoulos; C. Papanikolaou; G. Passalacqua; E. Pastor; M. Perrot; D. Plavec; Todor Popov; D. S. Postma; D. Price; N. Raffort; J. C. Reuzeau; J. M. Robine; F. Rodenas; Fabio Robusto; N. Roche; Antonino Romano; V. Romano; J. Rosado-Pinto; F. Roubille; F. Ruiz; D. Ryan; T. Salcedo; P. Schmid-Grendelmeier; H. Schulz; H. J. Schunemann; E. Serrano; Aziz Sheikh; M. Shields; N. Siafakas; N. Scichilone; I. Skrindo; H. A. Smit; S. Sourdet; O. Spranger; T. Sooronbaev; V. Sruk; P. J. Sterk; Ana Todo-Bom; J. Touchon; Donatella Tramontano; Massimo Triggiani; A. L. Valero; E. Valovirta; E. Van Ganse; M. Van Hage; Maarten Van Den Berge; Maria Teresa Ventura; I. Vergara; G. Vezzani; D. Vidal; G. Viegi; M. Wagemann; B. Whalley; N. Wilson; Panayiotis Yiallouros; M. Žagar; A. Zaidi; Mihaela Zidarn; E. J. Hoogerwerf; J. Usero; R. Zuffada; A. Senn; B. De Oliveira-Alves. Building bridges for innovation in ageing: Synergies between action groups of the EIP on AHA. The journal of nutrition, health & aging 2016, 21, 92 -104.
AMA StyleJean Bousquet, M. Bewick, A. Cano, P. Eklund, Giuseppe Fico, N. Goswami, N. A. Guldemond, D. Henderson, M. J. Hinkema, G. Liotta, A. Mair, W. Molloy, A. Monaco, I. Monsonis-Paya, A. Nizinska, H. Papadopoulos, A. Pavlickova, S. Pecorelli, Alexandra Prados-Torres, R. E. Roller-Wirnsberger, D. Somekh, Cecilia Vera Muñoz, F. Visser, J. Farrell, João Malva, Karen Andersen-Ranberg, T. Camuzat, A. M. Carriazo, G. Crooks, Z. Gutter, Guido Iaccarino, E. Manuel De Keenoy, G. Moda, Leocadio Rodríguez-Mañas, T. Vontetsianos, C. Abreu, J. Alonso, C. Alonso-Bouzon, Joel Ankri, M. T. Arredondo, F. Avolio, A. Bedbrook, A. Z. Białoszewski, H. Blain, R. Bourret, M. F. Cabrera-Umpierrez, A. Catala, Rónán O'Caoimh, M. Cesari, Niels Chavannes, J. Correia-Da-Sousa, T. Dedeu, M. Ferrando, M. Ferri, W. J. Fokkens, F. Garcia-Lizana, O. Guérin, Peter Hellings, T. Haahtela, M. Illario, M. C. Inzerilli, K. C. Lodrup Carlsen, P. Kardas, T. Keil, M. Maggio, A. Mendez-Zorrilla, E. Menditto, J. Mercier, J. P. Michel, R. Murray, M. Nogues, I. O’Byrne-Maguire, D. Pappa, A. S. Parent, M. Pastorino, Carlos Manuel Da Silva Robalo Cordeiro, B. Samolinski, P. Siciliano, Ana Teixeira, S. I. Tsartara, A. Valiulis, O. Vandenplas, Tuula Vasankari, B. Vellas, M. Vollenbroek-Hutten, M. Wickman, Arzu Yorgancıoglu, Torsten Zuberbier, M. Barbagallo, Giorgio Walter Canonica, L. Klimek, S. Maggi, W. Aberer, C. Akdis, I. M. Adcock, I. Agache, C. Albera, Federico Alonso-Trujillo, M. Angel Guarcia, I. Annesi-Maesano, Joao Luis Alves Apostolo, S. H. Arshad, V. Attalin, A. Avignon, C. Bachert, I. Baroni, E. Bel, M. Benson, C. Bescos, F. Blasi, Cristina Bárbara, K. C. Bergmann, P. L. Bernard, Sergio Bonini, P. J. Bousquet, B. Branchini, Chris Brightling, V. Bruguière, C. Bunu, A. Bush, Davide Caimmi, M. A. Calderon, G. Canovas, Victoria Cardona, A. Cesario, E. Chkhartishvili, R. Chiron, T. Chivato, K. F. Chung, M. D’Angelantonio, G. De Carlo, D. Cholley, F. Chorin, B. Combe, B. Compas, D. J. Costa, Elisio Costa, O. Coste, A. -L. Coupet, G. Crepaldi, Adnan Custovic, R. Dahl, S. E. Dahlen, Pascal Demoly, P. Devillier, A. Didier, Anh Tuan Dinh-Xuan, Ratko Djukanovic, D. Dokic, George Du Toit, R. Dubakiene, A. Dupeyron, R. Emuzyte, Alessandro Fiocchi, A. Wagner, M. Fletcher, João Fonseca, B. Fougère, A. Gamkrelidze, G. Garces, J. Garcia-Aymeric, B. Garcia-Zapirain, B. Gemicioğlu, C. Gouder, B. Hellquist-Dahl, I. Hermosilla-Gimeno, D. Héve, Carol Holland, Marc Humbert, M. Hyland, Sebastian Johnston, J. Just, M. Jutel, Igor Kaidashev, M. Kaitov, O. Kalayci, A. F. Kalyoncu, W. Keijser, Huib Kerstjens, J. Knezović, M. Kowalski, Gerard Koppelman, T. Kotska, M. Kovac, I. Kull, Piotr Kuna, V. Kvedariene, V. Lepore, W. Macnee, A. Magnan, I. Majer, P. Manning, Maura Marcucci, T. Marti, M. Masoli, E. Melen, N. Miculinic, F. Mihaltan, B. Milenkovic, J. Millot-Keurinck, H. Mlinarić, I. Momas, S. Montefort, M. Morais-Almeida, Teresa Moreno-Casbas, R. Mösges, J. Mullol, Rachel Nadif, M. Nalin, Esperanza Navarro-Pardo, K. Nekam, Gregory Ninot, D. Paccard, Sandra Cristina Cozinheiro Fidalgo Rafael Gamboa Pais, E. Palummeri, Petr Panzner, N. K. Papadopoulos, C. Papanikolaou, G. Passalacqua, E. Pastor, M. Perrot, D. Plavec, Todor Popov, D. S. Postma, D. Price, N. Raffort, J. C. Reuzeau, J. M. Robine, F. Rodenas, Fabio Robusto, N. Roche, Antonino Romano, V. Romano, J. Rosado-Pinto, F. Roubille, F. Ruiz, D. Ryan, T. Salcedo, P. Schmid-Grendelmeier, H. Schulz, H. J. Schunemann, E. Serrano, Aziz Sheikh, M. Shields, N. Siafakas, N. Scichilone, I. Skrindo, H. A. Smit, S. Sourdet, O. Spranger, T. Sooronbaev, V. Sruk, P. J. Sterk, Ana Todo-Bom, J. Touchon, Donatella Tramontano, Massimo Triggiani, A. L. Valero, E. Valovirta, E. Van Ganse, M. Van Hage, Maarten Van Den Berge, Maria Teresa Ventura, I. Vergara, G. Vezzani, D. Vidal, G. Viegi, M. Wagemann, B. Whalley, N. Wilson, Panayiotis Yiallouros, M. Žagar, A. Zaidi, Mihaela Zidarn, E. J. Hoogerwerf, J. Usero, R. Zuffada, A. Senn, B. De Oliveira-Alves. Building bridges for innovation in ageing: Synergies between action groups of the EIP on AHA. The journal of nutrition, health & aging. 2016; 21 (1):92-104.
Chicago/Turabian StyleJean Bousquet; M. Bewick; A. Cano; P. Eklund; Giuseppe Fico; N. Goswami; N. A. Guldemond; D. Henderson; M. J. Hinkema; G. Liotta; A. Mair; W. Molloy; A. Monaco; I. Monsonis-Paya; A. Nizinska; H. Papadopoulos; A. Pavlickova; S. Pecorelli; Alexandra Prados-Torres; R. E. Roller-Wirnsberger; D. Somekh; Cecilia Vera Muñoz; F. Visser; J. Farrell; João Malva; Karen Andersen-Ranberg; T. Camuzat; A. M. Carriazo; G. Crooks; Z. Gutter; Guido Iaccarino; E. Manuel De Keenoy; G. Moda; Leocadio Rodríguez-Mañas; T. Vontetsianos; C. Abreu; J. Alonso; C. Alonso-Bouzon; Joel Ankri; M. T. Arredondo; F. Avolio; A. Bedbrook; A. Z. Białoszewski; H. Blain; R. Bourret; M. F. Cabrera-Umpierrez; A. Catala; Rónán O'Caoimh; M. Cesari; Niels Chavannes; J. Correia-Da-Sousa; T. Dedeu; M. Ferrando; M. Ferri; W. J. Fokkens; F. Garcia-Lizana; O. Guérin; Peter Hellings; T. Haahtela; M. Illario; M. C. Inzerilli; K. C. Lodrup Carlsen; P. Kardas; T. Keil; M. Maggio; A. Mendez-Zorrilla; E. Menditto; J. Mercier; J. P. Michel; R. Murray; M. Nogues; I. O’Byrne-Maguire; D. Pappa; A. S. Parent; M. Pastorino; Carlos Manuel Da Silva Robalo Cordeiro; B. Samolinski; P. Siciliano; Ana Teixeira; S. I. Tsartara; A. Valiulis; O. Vandenplas; Tuula Vasankari; B. Vellas; M. Vollenbroek-Hutten; M. Wickman; Arzu Yorgancıoglu; Torsten Zuberbier; M. Barbagallo; Giorgio Walter Canonica; L. Klimek; S. Maggi; W. Aberer; C. Akdis; I. M. Adcock; I. Agache; C. Albera; Federico Alonso-Trujillo; M. Angel Guarcia; I. Annesi-Maesano; Joao Luis Alves Apostolo; S. H. Arshad; V. Attalin; A. Avignon; C. Bachert; I. Baroni; E. Bel; M. Benson; C. Bescos; F. Blasi; Cristina Bárbara; K. C. Bergmann; P. L. Bernard; Sergio Bonini; P. J. Bousquet; B. Branchini; Chris Brightling; V. Bruguière; C. Bunu; A. Bush; Davide Caimmi; M. A. Calderon; G. Canovas; Victoria Cardona; A. Cesario; E. Chkhartishvili; R. Chiron; T. Chivato; K. F. Chung; M. D’Angelantonio; G. De Carlo; D. Cholley; F. Chorin; B. Combe; B. Compas; D. J. Costa; Elisio Costa; O. Coste; A. -L. Coupet; G. Crepaldi; Adnan Custovic; R. Dahl; S. E. Dahlen; Pascal Demoly; P. Devillier; A. Didier; Anh Tuan Dinh-Xuan; Ratko Djukanovic; D. Dokic; George Du Toit; R. Dubakiene; A. Dupeyron; R. Emuzyte; Alessandro Fiocchi; A. Wagner; M. Fletcher; João Fonseca; B. Fougère; A. Gamkrelidze; G. Garces; J. Garcia-Aymeric; B. Garcia-Zapirain; B. Gemicioğlu; C. Gouder; B. Hellquist-Dahl; I. Hermosilla-Gimeno; D. Héve; Carol Holland; Marc Humbert; M. Hyland; Sebastian Johnston; J. Just; M. Jutel; Igor Kaidashev; M. Kaitov; O. Kalayci; A. F. Kalyoncu; W. Keijser; Huib Kerstjens; J. Knezović; M. Kowalski; Gerard Koppelman; T. Kotska; M. Kovac; I. Kull; Piotr Kuna; V. Kvedariene; V. Lepore; W. Macnee; A. Magnan; I. Majer; P. Manning; Maura Marcucci; T. Marti; M. Masoli; E. Melen; N. Miculinic; F. Mihaltan; B. Milenkovic; J. Millot-Keurinck; H. Mlinarić; I. Momas; S. Montefort; M. Morais-Almeida; Teresa Moreno-Casbas; R. Mösges; J. Mullol; Rachel Nadif; M. Nalin; Esperanza Navarro-Pardo; K. Nekam; Gregory Ninot; D. Paccard; Sandra Cristina Cozinheiro Fidalgo Rafael Gamboa Pais; E. Palummeri; Petr Panzner; N. K. Papadopoulos; C. Papanikolaou; G. Passalacqua; E. Pastor; M. Perrot; D. Plavec; Todor Popov; D. S. Postma; D. Price; N. Raffort; J. C. Reuzeau; J. M. Robine; F. Rodenas; Fabio Robusto; N. Roche; Antonino Romano; V. Romano; J. Rosado-Pinto; F. Roubille; F. Ruiz; D. Ryan; T. Salcedo; P. Schmid-Grendelmeier; H. Schulz; H. J. Schunemann; E. Serrano; Aziz Sheikh; M. Shields; N. Siafakas; N. Scichilone; I. Skrindo; H. A. Smit; S. Sourdet; O. Spranger; T. Sooronbaev; V. Sruk; P. J. Sterk; Ana Todo-Bom; J. Touchon; Donatella Tramontano; Massimo Triggiani; A. L. Valero; E. Valovirta; E. Van Ganse; M. Van Hage; Maarten Van Den Berge; Maria Teresa Ventura; I. Vergara; G. Vezzani; D. Vidal; G. Viegi; M. Wagemann; B. Whalley; N. Wilson; Panayiotis Yiallouros; M. Žagar; A. Zaidi; Mihaela Zidarn; E. J. Hoogerwerf; J. Usero; R. Zuffada; A. Senn; B. De Oliveira-Alves. 2016. "Building bridges for innovation in ageing: Synergies between action groups of the EIP on AHA." The journal of nutrition, health & aging 21, no. 1: 92-104.
Resveratrol, a dietary polyphenol, is under consideration as chemopreventive and chemotherapeutic agent for several diseases, including cancer. However, its mechanisms of action and its effects on non-tumor cells, fundamental to understand its real efficacy as chemopreventive agent, remain largely unknown. Proline-rich tyrosine kinase 2 (PYK2), a non-receptor tyrosine kinase acting as signaling mediator of different stimuli, behaves as tumor-suppressor in prostate. Since, PYK2 and RSV share several fields of interaction, including oxidative stress, we have investigated their functional relationship in human non-transformed prostate EPN cells and in their tumor-prone counterpart EPN-PKM, expressing a PYK2 dead-kinase mutant. We show that RSV has a strong biological activity in both cell lines, decreasing ROS production, inducing morphological changes and reversible growth arrest, and activating autophagy but not apoptosis. Interestingly, the PYK2 mutant increases basal ROS and autophagy levels, and modulates the intensity of RSV effects. In particular, the anti-oxidant effect of RSV is more potent in EPN than in EPN-PKM, whereas its anti-proliferative and pro-autophagic effects are more significant in EPN-PKM. Consistently, PYK2 depletion by RNAi replicates the effects of the PKM mutant. Taken together, our results reveal that PYK2 and RSV act on common cellular pathways and suggest that RSV effects on prostate cells may depend on mutational-state or expression levels of PYK2 that emerges as a possible mediator of RSV mechanisms of action. Moreover, the observation that resveratrol effects are reversible and not associated to apoptosis in tumor-prone EPN-PKM cells suggests caution for its use in humans.
Andrea Conte; Annamaria Kisslinger; Claudio Procaccini; Simona Paladino; Olimpia Oliviero; Francesca De Amicis; Deriggio Faicchia; Dominga Fasano; Marilena Caputo; Giuseppe Matarese; Giovanna Maria Pierantoni; Donatella Tramontano. Convergent Effects of Resveratrol and PYK2 on Prostate Cells. International Journal of Molecular Sciences 2016, 17, 1542 .
AMA StyleAndrea Conte, Annamaria Kisslinger, Claudio Procaccini, Simona Paladino, Olimpia Oliviero, Francesca De Amicis, Deriggio Faicchia, Dominga Fasano, Marilena Caputo, Giuseppe Matarese, Giovanna Maria Pierantoni, Donatella Tramontano. Convergent Effects of Resveratrol and PYK2 on Prostate Cells. International Journal of Molecular Sciences. 2016; 17 (9):1542.
Chicago/Turabian StyleAndrea Conte; Annamaria Kisslinger; Claudio Procaccini; Simona Paladino; Olimpia Oliviero; Francesca De Amicis; Deriggio Faicchia; Dominga Fasano; Marilena Caputo; Giuseppe Matarese; Giovanna Maria Pierantoni; Donatella Tramontano. 2016. "Convergent Effects of Resveratrol and PYK2 on Prostate Cells." International Journal of Molecular Sciences 17, no. 9: 1542.
// Francesca De Amicis 1, 2 , Carmela Guido 1, 2 , Marta Santoro 1 , Francesca Giordano 2 , Ada Donà 1, 2 , Pietro Rizza 2 , Michele Pellegrino 2 , Ida Perrotta 3 , Daniela Bonofiglio 1, 2 , Diego Sisci 1, 2 , Maria Luisa Panno 2 , Donatella Tramontano 4 , Saveria Aquila 1, 2, * , Sebastiano Andò 1, 2, * 1 Centro Sanitario, University of Calabria, Rende (CS), Italy 2 Department of Pharmacy, Health Science and Nutrition, University of Calabria, Rende (CS), Italy 3 DiBEST University of Calabria, Rende (CS), Italy 4 Department of Molecular Medicine and Medical Biotechnologies, University of Naples “Federico II”, Naples, Italy * Joint senior authors Correspondence to: Francesca De Amicis, email: [email protected] Sebastiano Andò, email: [email protected] Keywords: progesterone, Bcl-2, cell cycle arrest, pRb, p16 Received: February 03, 2016 Accepted: July 09, 2016 Published: July 23, 2016 ABSTRACT Loss of progesterone-receptors (PR) expression is associated with breast cancer progression. Herein we provide evidence that OHPg/PR-B through Beclin-1 evoke autophagy-senescence transition, in breast cancer cells. Specifically, OHPg increases Beclin-1 expression through a transcriptional mechanism due to the occupancy of Beclin-1 promoter by PR-B, together with the transcriptional coactivator SRC-2. This complex binds at a canonical half progesterone responsive element, which is fundamental for OHPg effects, as shown by site-directed mutagenesis. Beside, OHPg via non-genomic action rapidly activates JNK, which phosphorylates Bcl-2, producing the functional release from Beclin-1 interaction. This is not linked to an efficient autophagic flux, since p62 levels, marker of degradation via lysosomes, were not reduced after sustained OHPg stimulus. Instead, the cell cycle inhibitor p27 was induced, together with an irreversible G1 arrest, hallmark of cellular senescence. Specifically the increase of senescence-associated β-galactosidase activity was blocked by Bcl-2 siRNA but also by Beclin-1 siRNA. Collectively these findings support the importance of PR-B expression in breast cancer cells, thus targeting PR-B may be a useful strategy to provide additional approaches to existing therapies for breast cancer patients.
Francesca De Amicis; Carmela Guido; Marta Santoro; Francesca Giordano; Ada Donà; Pietro Rizza; Michele Pellegrino; Ida Perrotta; Daniela Bonofiglio; Diego Sisci; Maria Luisa Panno; Donatella Tramontano; Saveria Aquila; Sebastiano Andò. Ligand activated progesterone receptor B drives autophagy-senescence transition through a Beclin-1/Bcl-2 dependent mechanism in human breast cancer cells. Oncotarget 2016, 7, 57955 -57969.
AMA StyleFrancesca De Amicis, Carmela Guido, Marta Santoro, Francesca Giordano, Ada Donà, Pietro Rizza, Michele Pellegrino, Ida Perrotta, Daniela Bonofiglio, Diego Sisci, Maria Luisa Panno, Donatella Tramontano, Saveria Aquila, Sebastiano Andò. Ligand activated progesterone receptor B drives autophagy-senescence transition through a Beclin-1/Bcl-2 dependent mechanism in human breast cancer cells. Oncotarget. 2016; 7 (36):57955-57969.
Chicago/Turabian StyleFrancesca De Amicis; Carmela Guido; Marta Santoro; Francesca Giordano; Ada Donà; Pietro Rizza; Michele Pellegrino; Ida Perrotta; Daniela Bonofiglio; Diego Sisci; Maria Luisa Panno; Donatella Tramontano; Saveria Aquila; Sebastiano Andò. 2016. "Ligand activated progesterone receptor B drives autophagy-senescence transition through a Beclin-1/Bcl-2 dependent mechanism in human breast cancer cells." Oncotarget 7, no. 36: 57955-57969.
Social isolation and exclusion are associated with poor health status and premature death. A number of related isolation factors, inadequate transportation system and restrictions in individuals’ life space, have been associated with malnutrition in older adults. Since eating is a social event, isolation can have a negative effect on nutrition. Cultural involvement and participation in interactive activities are essential tools to fight social isolation, and they can counteract the detrimental effects of social isolation on health. To provide data supporting the hypothesis that encouraging participation might represent an innovative preventive and health promoting strategy for healthy living and aging, we developed anad hocquestionnaire to investigate the relationship between cultural participation, well-being, and resilience in a sample of residents in the metropolitan area of Naples. The questionnaire includes a question on adherence to diet or to a special nutritional regimen; in addition, the participants are asked to mention their height and weight. We investigated the relationship between BMI, adherence to diet, and perceived well-being (PWB) and resilience in a sample of 571 subjects over 60 years of age. Here, we present evidence that engagement into social and cultural activities is associated with higher well-being and resilience, in particular in females over 60 years of age.
Antonio Rapacciuolo; Pasquale Perrone Filardi; Rosario Cuomo; Vincenzo Mauriello; Maria Quarto; Annamaria Kisslinger; Gianluigi Savarese; Maddalena Illario; Donatella Tramontano. The Impact of Social and Cultural Engagement and Dieting on Well-Being and Resilience in a Group of Residents in the Metropolitan Area of Naples. Journal of Aging Research 2016, 2016, 1 -11.
AMA StyleAntonio Rapacciuolo, Pasquale Perrone Filardi, Rosario Cuomo, Vincenzo Mauriello, Maria Quarto, Annamaria Kisslinger, Gianluigi Savarese, Maddalena Illario, Donatella Tramontano. The Impact of Social and Cultural Engagement and Dieting on Well-Being and Resilience in a Group of Residents in the Metropolitan Area of Naples. Journal of Aging Research. 2016; 2016 ():1-11.
Chicago/Turabian StyleAntonio Rapacciuolo; Pasquale Perrone Filardi; Rosario Cuomo; Vincenzo Mauriello; Maria Quarto; Annamaria Kisslinger; Gianluigi Savarese; Maddalena Illario; Donatella Tramontano. 2016. "The Impact of Social and Cultural Engagement and Dieting on Well-Being and Resilience in a Group of Residents in the Metropolitan Area of Naples." Journal of Aging Research 2016, no. : 1-11.
(Immunity 44, 406–421, February 16, 2016)
Claudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; PietroLuigi Mauri; Giuseppe Matarese. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity 2016, 44, 712 .
AMA StyleClaudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani, Deriggio Faicchia, Gianni Marone, Donatella Tramontano, Marco Corona, Carlo Alviggi, Antonio Porcellini, Antonio La Cava, PietroLuigi Mauri, Giuseppe Matarese. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity. 2016; 44 (3):712.
Chicago/Turabian StyleClaudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; PietroLuigi Mauri; Giuseppe Matarese. 2016. "The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements." Immunity 44, no. 3: 712.
Summary Human CD4+CD25hiFoxp3+CD127− Treg and CD4+CD25−Foxp3− Tconv cell functions are governed by their metabolic requirements. Here we report a comprehensive comparative analysis between ex vivo human Treg and Tconv cells that comprises analyses of the proteomic networks in subcellular compartments. We identified a dominant proteomic signature at the metabolic level that primarily impacted the highly-tuned balance between glucose and fatty-acid oxidation in the two cell types. Ex vivo Treg cells were highly glycolytic while Tconv cells used predominantly fatty-acid oxidation (FAO). When cultured in vitro, Treg cells engaged both glycolysis and FAO to proliferate, while Tconv cell proliferation mainly relied on glucose metabolism. Our unbiased proteomic analysis provides a molecular picture of the impact of metabolism on ex vivo human Treg versus Tconv cell functions that might be relevant for therapeutic manipulations of these cells.
Claudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; PietroLuigi Mauri; Giuseppe Matarese. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity 2016, 44, 406 -421.
AMA StyleClaudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani, Deriggio Faicchia, Gianni Marone, Donatella Tramontano, Marco Corona, Carlo Alviggi, Antonio Porcellini, Antonio La Cava, PietroLuigi Mauri, Giuseppe Matarese. The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements. Immunity. 2016; 44 (2):406-421.
Chicago/Turabian StyleClaudio Procaccini; Fortunata Carbone; Dario Di Silvestre; Francesca Brambilla; Veronica De Rosa; Mario Galgani; Deriggio Faicchia; Gianni Marone; Donatella Tramontano; Marco Corona; Carlo Alviggi; Antonio Porcellini; Antonio La Cava; PietroLuigi Mauri; Giuseppe Matarese. 2016. "The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements." Immunity 44, no. 2: 406-421.
UVB radiation causes about 90% of non-melanoma skin cancers by damaging DNA either directly or indirectly by increasing levels of reactive oxygen species (ROS). Skin, chronically exposed to both endogenous and environmental pro-oxidant agents, contains a well-organised system of chemical and enzymatic antioxidants. However, increased or prolonged free radical action can overwhelm ROS defence mechanisms, contributing to the development of cutaneous diseases. Thus, new strategies for skin protection comprise the use of food antioxidants to counteract oxidative stress. Resveratrol, a phytoalexin from grape, has gained a great interest for its ability to influence several biological mechanisms like redox balance, cell proliferation, signal transduction pathways, immune and inflammatory response. Therefore, the potential of resveratrol to modify skin cell response to UVB exposure could turn out to be a useful option to protect skin from sunlight-induced degenerative diseases. To investigate into this matter, HaCaT cells, a largely used model for human skin keratinocytes, were treated with 25 or 100 µM resveratrol for 2 and 24 hours prior to UVB irradiation (10 to 100 mJ/cm2). Cell viability and molecular markers of proliferation, oxidative stress, apoptosis, and autophagy were analyzed. In HaCaT cells resveratrol pretreatment: reduces UVB-induced ROS formation, enhances the detrimental effect of UVB on HaCaT cell vitality, increases UVB-induced caspase 8, PARP cleavage, and induces autophagy. These findings suggest that resveratrol could exert photochemopreventive effects by enhancing UVB-induced apoptosis and by inducing autophagy, thus reducing the odds that damaged cells could escape programmed cell death and initiate malignant transformation.
Nicoletta Vitale; Annamaria Kisslinger; Simona Paladino; Claudio Procaccini; Giuseppe Matarese; Giovanna Maria Pierantoni; Francesco Paolo Mancini; Donatella Tramontano. Resveratrol Couples Apoptosis with Autophagy in UVB-Irradiated HaCaT Cells. PLOS ONE 2013, 8, e80728 .
AMA StyleNicoletta Vitale, Annamaria Kisslinger, Simona Paladino, Claudio Procaccini, Giuseppe Matarese, Giovanna Maria Pierantoni, Francesco Paolo Mancini, Donatella Tramontano. Resveratrol Couples Apoptosis with Autophagy in UVB-Irradiated HaCaT Cells. PLOS ONE. 2013; 8 (11):e80728.
Chicago/Turabian StyleNicoletta Vitale; Annamaria Kisslinger; Simona Paladino; Claudio Procaccini; Giuseppe Matarese; Giovanna Maria Pierantoni; Francesco Paolo Mancini; Donatella Tramontano. 2013. "Resveratrol Couples Apoptosis with Autophagy in UVB-Irradiated HaCaT Cells." PLOS ONE 8, no. 11: e80728.
Well‐differentiated papillary and follicular thyroid carcinoma are the most frequent types of thyroid cancer and the prognosis is generally favorable however, a number of patients develops recurrences. Epigallocatechin‐3‐gallate (EGCG), a major catechin in green tea, was shown to possess remarkable therapeutic potential against various types of human cancers, although data on thyroid cancer cells are still lacking. The aim of this study was to investigate the effect of EGCG on the proliferation and motility of human thyroid papillary (FB‐2) and follicular (WRO) carcinoma cell lines. Our results demonstrate that EGCG (10, 40, 60 μM) treatment inhibited the growth of FB‐2 and WRO cells in a dose‐dependent manner. These changes were associated with reduced cyclin D1, increased p21 and p53 expression. Furthermore, EGCG suppressed phosphorylation of AKT and ERK1/2. In addition EGCG treatment results in reduction of cell motility and migration. Changes in motility and migration in FB‐2 were associated with modulation in the expression of several proteins involved in cell adhesion and reorganization of actin cytoskeleton. After 24 h EGCG caused an increase of the E‐cadherin expression and a concomitant decrease of SNAIL, ZEB and the basic helix–loop–helix transcription factor TWIST. Besides expression of Vimentin, N‐cadherin and α5‐integrin was down‐regulated. These data well correlate with a reduction of MMP9 activity as evidenced by gelatin zymography. Our findings support the inhibitory role of EGCG on thyroid cancer cell proliferation and motility with concomitant loss of epithelial‐to‐mesenchymal cell transition markers. J. Cell. Physiol. 228: 2054–2062, 2013.
Francesca De Amicis; Anna Perri; Donatella Vizza; Alessandra Russo; Maria Luisa Panno; Daniela Bonofiglio; Cinzia Giordano; Loredana Mauro; Saveria Aquila; Donatella Tramontano; Sebastiano Andò. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. Journal of Cellular Physiology 2013, 228, 2054 -2062.
AMA StyleFrancesca De Amicis, Anna Perri, Donatella Vizza, Alessandra Russo, Maria Luisa Panno, Daniela Bonofiglio, Cinzia Giordano, Loredana Mauro, Saveria Aquila, Donatella Tramontano, Sebastiano Andò. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. Journal of Cellular Physiology. 2013; 228 (10):2054-2062.
Chicago/Turabian StyleFrancesca De Amicis; Anna Perri; Donatella Vizza; Alessandra Russo; Maria Luisa Panno; Daniela Bonofiglio; Cinzia Giordano; Loredana Mauro; Saveria Aquila; Donatella Tramontano; Sebastiano Andò. 2013. "Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines." Journal of Cellular Physiology 228, no. 10: 2054-2062.
Scope Exposure of the breast to estrogens and other sex hormones is an important cancer risk factor and estrogen receptor downregulators are attracting significant clinical interest. Epigallocatechin gallate (EGCG), a polyphenolic compound found in green tea, has gained considerable attention for its antitumor properties. Here we aimed to investigate the molecular mechanisms through which EGCG regulates ER‐α expression in ER+ PR+ breast cancer cells. Material and methods Western blotting analysis, real‐time PCR, and transient transfections of deletion fragments of the ER‐α gene promoter show that EGCG downregulates ER‐α protein, mRNA, and gene promoter activity with a concomitant reduction of ER‐α genomic and nongenomic signal. These events occur through p38MAPK/CK2 activation, causing the release from Hsp90 of progesterone receptor B (PR‐B) and its consequent nuclear translocation as evidenced by immunofluorescence studies. EMSA, and ChIP assay reveal that, upon EGCG treatment, PR‐B is recruited at the half‐PRE site on ER‐α promoter. This is concomitant with the formation of a corepressor complex containing NCoR and HDAC1 while RNA polymerase II is displaced. The events are crucially mediated by PR‐B isoform, since they are abrogated with PR‐B siRNA. Conclusion Our data provide evidence for a mechanism by which EGCG downregulates ER‐α and explains the inhibitory action of EGCG on the proliferation of ER+ PR+ cancer cells tested. We suggest that the EGCG/PR‐B signaling should be further exploited for clinical approach.
Francesca De Amicis; Alessandra Russo; Paola Avena; Marta Santoro; Adele Vivacqua; Daniela Bonofiglio; Loredana Mauro; Saveria Aquila; Donatella Tramontano; Suzanne Aw Fuqua; Sebastiano Andò. In vitro mechanism for downregulation of ER-α expression by epigallocatechin gallate in ER+/PR+ human breast cancer cells. Molecular Nutrition & Food Research 2013, 57, 840 -853.
AMA StyleFrancesca De Amicis, Alessandra Russo, Paola Avena, Marta Santoro, Adele Vivacqua, Daniela Bonofiglio, Loredana Mauro, Saveria Aquila, Donatella Tramontano, Suzanne Aw Fuqua, Sebastiano Andò. In vitro mechanism for downregulation of ER-α expression by epigallocatechin gallate in ER+/PR+ human breast cancer cells. Molecular Nutrition & Food Research. 2013; 57 (5):840-853.
Chicago/Turabian StyleFrancesca De Amicis; Alessandra Russo; Paola Avena; Marta Santoro; Adele Vivacqua; Daniela Bonofiglio; Loredana Mauro; Saveria Aquila; Donatella Tramontano; Suzanne Aw Fuqua; Sebastiano Andò. 2013. "In vitro mechanism for downregulation of ER-α expression by epigallocatechin gallate in ER+/PR+ human breast cancer cells." Molecular Nutrition & Food Research 57, no. 5: 840-853.
Agents to counteract acquired resistance to hormonal therapy for breast cancer would substantially enhance the long-term benefits of hormonal therapy. In the present study, we demonstrate how resveratrol (Res) inhibits human breast cancer cell proliferation, including MCF-7 tamoxifen-resistant cells (IC50 values for viability were in the 30–45 μM range). We show that Res, through p38MAPK phosphorylation, causes induction of p53, which recruits at the estrogen receptor α (ERα) proximal promoter, leading to an inhibition of ERα expression in terms of mRNA and protein content. These events appear specifically p53 dependent, since they are drastically abrogated with p53-targeting siRNA. Coimmunoprecipitation assay showed specific interaction between p53, the Sin3A corepressor, and histone deacetylase 1 (HDAC1), which was phosphorylated. The enhancement of the tripartite complex p53/Sin3A/HDAC1, together with NF-Y on Res treatment, was confirmed by chromatin immunoprecipitation analyses, with a concomitant release of Sp1 and RNA polymerase II, thereby inhibiting the cell transcriptional machinery. The persistence of such effects in MCF-7 tamoxifen-resistant cells at a higher extent than parental MCF-7 cells addresses how Res may be considered a useful pharmacological tool to be exploited in the adjuvant settings for treatment of breast cancer developing hormonal resistance.—De Amicis, F., Giordano, F., Vivacqua, A., Pellegrino, M., Panno, M. L., Tramontano, D., Fuqua, S. A. W., Andò, S. Resveratrol, through NF-Y/p53/Sin3/HDAC1 complex phosphorylation, inhibits estrogen receptor α gene expression via p38MAPK/CK2 signaling in human breast cancer cells. FASEB J. 25, 3695–3707 (2011). www.fasebj.org
Francesca De Amicis; Francesca Giordano; Adele Vivacqua; Michele Pellegrino; Maria Luisa Panno; Donatella Tramontano; Suzanne A. W. Fuqua; Sebastiano Andò. Resveratrol, through NF‐Y/p53/Sin3/HDAC1 complex phosphorylation, inhibits estrogen receptor α gene expression via p38 MAPK /CK2 signaling in human breast cancer cells. The FASEB Journal 2011, 25, 3695 -3707.
AMA StyleFrancesca De Amicis, Francesca Giordano, Adele Vivacqua, Michele Pellegrino, Maria Luisa Panno, Donatella Tramontano, Suzanne A. W. Fuqua, Sebastiano Andò. Resveratrol, through NF‐Y/p53/Sin3/HDAC1 complex phosphorylation, inhibits estrogen receptor α gene expression via p38 MAPK /CK2 signaling in human breast cancer cells. The FASEB Journal. 2011; 25 (10):3695-3707.
Chicago/Turabian StyleFrancesca De Amicis; Francesca Giordano; Adele Vivacqua; Michele Pellegrino; Maria Luisa Panno; Donatella Tramontano; Suzanne A. W. Fuqua; Sebastiano Andò. 2011. "Resveratrol, through NF‐Y/p53/Sin3/HDAC1 complex phosphorylation, inhibits estrogen receptor α gene expression via p38 MAPK /CK2 signaling in human breast cancer cells." The FASEB Journal 25, no. 10: 3695-3707.
Please cite this paper as: Resveratrol regulates p66Shc activation in HaCaT cells. Experimental Dermatology 2010. Skin is exposed to both endogenous and environmental oxidant agents, leading to the harmful generation of reactive oxygen species. Particular interest has been pointed on plant antioxidants, such as resveratrol, because of their wide-ranging biological activity and clinical potential. Resveratrol exerts antioxidant, metabolism-regulating and pro-apoptotic/anti-cancer effects on a variety of experimental models and has been suggested to protect skin from ultraviolet-induced photodamaging and photoaging. In parallel, also the biological significance of p66Shc, a member of the Src Homologue and Collagene homologue family with redox activity, is getting further attention. Because of the striking intersection among the activities of resveratrol with those of p66Shc, we investigated whether resveratrol would activate p66Shc in human immortalised keratinocytes (HaCaT cells), a well known and largely used model for skin keratinocytes. HaCaT cells were treated with resveratrol (10-150 mum) for different times. The effect of resveratrol on the proliferation of HaCaT cells and the activation of ERK1/2, AKT, and p66Shc was investigated by cell counting, fluorescence-activated cell sorting, and western blot analysis of total or immunoprecipitated cell extracts. In HaCaT cells, resveratrol induces dose- and time-dependent growth arrest, p66Shc-Ser36 phosphorylation, ERK1/2 phosphorylation and AKT dephosphorylation. Finally, we showed that resveratrol-induced p66Shc-Ser36 phosphorylation is dependent on ERK1/2 activation. Interestingly, these resveratrol-induced molecular effects were associated with reduced adhesion and reversible growth arrest rather than cell death pathways. This is the first evidence linking resveratrol with p66Shc and suggests that p66Shc may contribute to the effect of resveratrol on cell proliferation and function in the outermost layer of the skin
Gabriella Fabbrocini; Annamaria Kisslinger; Paola Iannelli; Nicoletta Vitale; Claudio Procaccini; Giuseppina Sparaneo; Paolo Chieffi; Fabio Ayala; Francesco Paolo Mancini; Donatella Tramontano. Resveratrol regulates p66Shc activation in HaCaT cells. Experimental Dermatology 2010, 19, 895 -903.
AMA StyleGabriella Fabbrocini, Annamaria Kisslinger, Paola Iannelli, Nicoletta Vitale, Claudio Procaccini, Giuseppina Sparaneo, Paolo Chieffi, Fabio Ayala, Francesco Paolo Mancini, Donatella Tramontano. Resveratrol regulates p66Shc activation in HaCaT cells. Experimental Dermatology. 2010; 19 (10):895-903.
Chicago/Turabian StyleGabriella Fabbrocini; Annamaria Kisslinger; Paola Iannelli; Nicoletta Vitale; Claudio Procaccini; Giuseppina Sparaneo; Paolo Chieffi; Fabio Ayala; Francesco Paolo Mancini; Donatella Tramontano. 2010. "Resveratrol regulates p66Shc activation in HaCaT cells." Experimental Dermatology 19, no. 10: 895-903.
Prostatic cancer may remain organ-confined indefinitely; in a number of patients, however it gives rise to clinical symptoms and death. The biological behaviour of this tumour mostly remains difficult to predict. A promising tool for diagnosis and prognosis of some human tumours is the chromatin assembly factor-1 (CAF-1), involved in the control of higher order chromatin organization. The aim was to explore the role of CAF-1/p60 protein as a new prognostic marker for prostatic cancer.The expression of CAF-1/p60 was evaluated by immunohistochemistry and Western blotting in a selected series of prostatic cancers and in prostatic cancer cell lines. Results were compared with clinicopathological data and outcome of patients. CAF-1/p60 was expressed in all cases, with a linear increase from low-grade tumours (Gleason score 7). By comparing results with follow-up data, a significant association between overexpression of CAF-1/p60 and unfavourable behaviour of prostatic cancer emerged, and its predictive value was independent of classical prognostic factors.In our series of cases, overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis. CAF-1/p60 has a potential role as a new reliable prognostic biomarker for prostatic cancer.
Stefania Staibano; Massimo Mascolo; Francesco Paolo Mancini; Annamaria Kisslinger; Gaetano Salvatore; Maria Di Benedetto; Paolo Chieffi; Vincenzo Altieri; Domenico Prezioso; Gennaro Ilardi; Gaetano DE Rosa; Donatella Tramontano. Overexpression of chromatin assembly factor-1 (CAF-1) p60 is predictive of adverse behaviour of prostatic cancer. Histopathology 2009, 54, 580 -589.
AMA StyleStefania Staibano, Massimo Mascolo, Francesco Paolo Mancini, Annamaria Kisslinger, Gaetano Salvatore, Maria Di Benedetto, Paolo Chieffi, Vincenzo Altieri, Domenico Prezioso, Gennaro Ilardi, Gaetano DE Rosa, Donatella Tramontano. Overexpression of chromatin assembly factor-1 (CAF-1) p60 is predictive of adverse behaviour of prostatic cancer. Histopathology. 2009; 54 (5):580-589.
Chicago/Turabian StyleStefania Staibano; Massimo Mascolo; Francesco Paolo Mancini; Annamaria Kisslinger; Gaetano Salvatore; Maria Di Benedetto; Paolo Chieffi; Vincenzo Altieri; Domenico Prezioso; Gennaro Ilardi; Gaetano DE Rosa; Donatella Tramontano. 2009. "Overexpression of chromatin assembly factor-1 (CAF-1) p60 is predictive of adverse behaviour of prostatic cancer." Histopathology 54, no. 5: 580-589.
In cultured prostate cancer cells cAMP blocks proliferation and induces neuroendocrine differentiation. Pyk2 expression inversely correlates with malignancy of prostate cancer. The aim of this study was to investigate the interaction between cAMP and Pyk2 in the prostate. EPN cells, a line derived from human normal prostate expressing Pyk2, and EPN-PKM3 cells, an EPN clone bearing a Pyk2 kinase-negative mutant, were adopted as model system. cAMP inhibited cell growth in both prostate cell lines, and activated Pyk2, but not ERK1/2, in EPN cells. cAMP treatment, abolished the activation of AKT1, an important component of the pro-survival pathway, in the EPN cells but not in EPN-PKM3 cells. Finally, upon cAMP treatment, EPN and EPN-PKM3 cells exhibited different expression patterns of HOX genes, an important network controlling cell identity. These data demonstrated for the first time that Pyk2 and cAMP interact in regulating prostate cell functions and in "keeping" prostate identity.
Annamaria Kisslinger; Monica Cantile; Giuseppina Sparaneo; Nicolas Vitale; Gabriella Fabbrocini; Paolo Chieffi; Clemente Cillo; Francesco P. Mancini; Donatella Tramontano. cAMP and Pyk2 interact to regulate prostate cells proliferation and function. Cancer Biology & Therapy 2009, 8, 236 -242.
AMA StyleAnnamaria Kisslinger, Monica Cantile, Giuseppina Sparaneo, Nicolas Vitale, Gabriella Fabbrocini, Paolo Chieffi, Clemente Cillo, Francesco P. Mancini, Donatella Tramontano. cAMP and Pyk2 interact to regulate prostate cells proliferation and function. Cancer Biology & Therapy. 2009; 8 (3):236-242.
Chicago/Turabian StyleAnnamaria Kisslinger; Monica Cantile; Giuseppina Sparaneo; Nicolas Vitale; Gabriella Fabbrocini; Paolo Chieffi; Clemente Cillo; Francesco P. Mancini; Donatella Tramontano. 2009. "cAMP and Pyk2 interact to regulate prostate cells proliferation and function." Cancer Biology & Therapy 8, no. 3: 236-242.