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Dr. Sandra Díaz-Sánchez
Instituto de Investigación en Recursos Cinegéticos (IREC-CSIC), University of Castilla-La Mancha, 13005 Ciudad Real, Spain

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Research Keywords & Expertise

0 Animal Health
0 Metagenomics
0 vector-borne diseases
0 Metagenome Analysis
0 Host-microbiome interactions

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Animal Health
vector-borne diseases

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Short Biography

In 2007, I started my Ph.D. at the Institute of Game and Wildlife Research (IREC-UCLM-CSIC), for which I obtained a predoctoral fellowship (FPI program, Regional Government of Castilla-La Mancha). I defended my Ph.D. Thesis in January 2012, which was qualified with the Excellent Cum Laude distinction. In 2012, I gained a postdoctoral position at the Institute of Agriculture (UTIA) at the University of Tennessee, where I worked for 3 years within Dr. Hanning´s Molecular Microbiology Lab. During this period I deepen developed my interest on the composition of bacterial microbial communities, their functional capacities and their physiological interactions with their hosts. In 2015, I returned to the SaBio group at the Institute of Game and Wildlife Research (IREC-UCLM-CSIC) with a postdoctoral research position within an European research project (COMPARE, EU) and then in 2016 with a Juan de la Cierva Incorporación fellowship (MINECO). In these 5 years I was involved in 4 research projects, and produced 12 peer review publications (7 as 1st author), 2 book chapters and co-supervised one doctoral thesis. In 2018 I got a Diploma in Bioinformatics (University Pablo Olavide) and participated teaching to masters students (MUIBARC, IREC). In July 2019, I started to lead as a Principal Investigator the research project, IMpROVE, funded by the Regional Govern of Castilla-La Mancha Talent Retention Program, which implement metagenomic tools to investigate diversity and function of tick

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Journal article
Published: 30 June 2021 in Pharmaceuticals
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Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases.

ACS Style

Iván Pacheco; Sandra Díaz-Sánchez; Marinela Contreras; Margarita Villar; Alejandro Cabezas-Cruz; Christian Gortázar; José de la Fuente. Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis. Pharmaceuticals 2021, 14, 635 .

AMA Style

Iván Pacheco, Sandra Díaz-Sánchez, Marinela Contreras, Margarita Villar, Alejandro Cabezas-Cruz, Christian Gortázar, José de la Fuente. Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis. Pharmaceuticals. 2021; 14 (7):635.

Chicago/Turabian Style

Iván Pacheco; Sandra Díaz-Sánchez; Marinela Contreras; Margarita Villar; Alejandro Cabezas-Cruz; Christian Gortázar; José de la Fuente. 2021. "Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis." Pharmaceuticals 14, no. 7: 635.

Journal article
Published: 19 April 2021 in Viruses
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Coronavirus-like organisms have been previously identified in Arthropod ectoparasites (such as ticks and unfed cat flea). Yet, the question regarding the possible role of these arthropods as SARS-CoV-2 passive/biological transmission vectors is still poorly explored. In this study, we performed in silico structural and binding energy calculations to assess the risks associated with possible ectoparasite transmission. We found sufficient similarity between ectoparasite ACE and human ACE2 protein sequences to build good quality 3D-models of the SARS-CoV-2 Spike:ACE complex to assess the impacts of ectoparasite mutations on complex stability. For several species (e.g., water flea, deer tick, body louse), our analyses showed no significant destabilisation of the SARS-CoV-2 Spike:ACE complex, suggesting these species would bind the viral Spike protein. Our structural analyses also provide structural rationale for interactions between the viral Spike and the ectoparasite ACE proteins. Although we do not have experimental evidence of infection in these ectoparasites, the predicted stability of the complex suggests this is possible, raising concerns of a possible role in passive transmission of the virus to their human hosts.

ACS Style

Su Lam; Paul Ashford; Sandra Díaz-Sánchez; Margarita Villar; Christian Gortázar; José de la Fuente; Christine Orengo. Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE. Viruses 2021, 13, 708 .

AMA Style

Su Lam, Paul Ashford, Sandra Díaz-Sánchez, Margarita Villar, Christian Gortázar, José de la Fuente, Christine Orengo. Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE. Viruses. 2021; 13 (4):708.

Chicago/Turabian Style

Su Lam; Paul Ashford; Sandra Díaz-Sánchez; Margarita Villar; Christian Gortázar; José de la Fuente; Christine Orengo. 2021. "Arthropod Ectoparasites Have Potential to Bind SARS-CoV-2 via ACE." Viruses 13, no. 4: 708.

Short communication
Published: 14 December 2020 in Ticks and Tick-borne Diseases
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Exploring tick associations with complex microbial communities and single-microbial partners, especially intracellular symbionts, has become crucial to understand tick biology. Of particular interest are the underlying interactions with biological consequences i.e. tick fitness, vector competence. In this study, we first sequenced the 16S rRNA bacterial phylogenetic marker in adult male ticks of Hyalomma lusitanicum collected from 5 locations in the province of Cáceres to explore the composition of its microbial community. Overall, 16S rRNA sequencing results demonstrated that the microbial community of H. lusitanicum is mostly dominated by Francisella-like endosymbionts (FLEs) (ranging from 52% to 99% of relative abundance) suggesting it is a key taxon within the microbial community and likely a primary endosymbiont. However, further research is required to explore the mechanisms underlying the interaction between FLEs and H. lusitanicum.

ACS Style

Sandra Díaz-Sánchez; Alberto Moraga Fernández; Miguel A. Habela; Rafael Calero-Bernal; Isabel G. Fernández de Mera; José de la Fuente. Microbial community of Hyalomma lusitanicum is dominated by Francisella-like endosymbiont. Ticks and Tick-borne Diseases 2020, 12, 101624 .

AMA Style

Sandra Díaz-Sánchez, Alberto Moraga Fernández, Miguel A. Habela, Rafael Calero-Bernal, Isabel G. Fernández de Mera, José de la Fuente. Microbial community of Hyalomma lusitanicum is dominated by Francisella-like endosymbiont. Ticks and Tick-borne Diseases. 2020; 12 (2):101624.

Chicago/Turabian Style

Sandra Díaz-Sánchez; Alberto Moraga Fernández; Miguel A. Habela; Rafael Calero-Bernal; Isabel G. Fernández de Mera; José de la Fuente. 2020. "Microbial community of Hyalomma lusitanicum is dominated by Francisella-like endosymbiont." Ticks and Tick-borne Diseases 12, no. 2: 101624.

Original research article
Published: 10 March 2020 in Frontiers in Cellular and Infection Microbiology
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Ticks are arthropod ectoparasite vectors of pathogens and the cause of allergic reactions affecting human health worldwide. In humans, tick bites can induce high levels of immunoglobulin E antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate anaphylactic reactions known as the alpha-Gal syndrome (AGS) or red meat allergy. In this study, a new animal model was developed using zebrafish for the study of allergic reactions and the immune mechanisms in response to tick salivary biogenic substances and red meat consumption. The results showed allergic hemorrhagic anaphylactic-type reactions and abnormal behavior patterns likely in response to tick salivary toxic and anticoagulant biogenic compounds different from α-Gal. However, the results showed that only zebrafish previously exposed to tick saliva developed allergic reactions to red meat consumption with rapid desensitization and tolerance. These allergic reactions were associated with tissue-specific Toll-like receptor-mediated responses in types 1 and 2 T helper cells (TH1 and TH2) with a possible role for basophils in response to tick saliva. These results support previously proposed immune mechanisms triggering the AGS and provided evidence for new mechanisms also potentially involved in the AGS. These results support the use of the zebrafish animal model for the study of the AGS and other tick-borne allergies.

ACS Style

Marinela Contreras; Iván Pacheco; Pilar Alberdi; Sandra Diaz Sanchez; Sara Artigas Jerónimo; Lourdes Mateos-Hernández; Margarita Villar; Alejandro Cabezas-Cruz; José De La Fuente. Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model. Frontiers in Cellular and Infection Microbiology 2020, 10, 78 .

AMA Style

Marinela Contreras, Iván Pacheco, Pilar Alberdi, Sandra Diaz Sanchez, Sara Artigas Jerónimo, Lourdes Mateos-Hernández, Margarita Villar, Alejandro Cabezas-Cruz, José De La Fuente. Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model. Frontiers in Cellular and Infection Microbiology. 2020; 10 ():78.

Chicago/Turabian Style

Marinela Contreras; Iván Pacheco; Pilar Alberdi; Sandra Diaz Sanchez; Sara Artigas Jerónimo; Lourdes Mateos-Hernández; Margarita Villar; Alejandro Cabezas-Cruz; José De La Fuente. 2020. "Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model." Frontiers in Cellular and Infection Microbiology 10, no. : 78.

Journal article
Published: 30 December 2019 in International Journal of Food Microbiology
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The interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design, close to fresh produce, and growers frequently move between the two production environments. The primary objectives of this study were to 1) determine the transmission of STEC or enteric pathogens from small and large animal herds or operations to fresh produce on sustainable farms in TN and NC, 2) identify the possible sources that impact transmission of AMR E. coli, specifically STEC on these systems, and 3) WGS to characterize recovered E. coli from these sources. Samples were collected from raw and composted manure, environment, and produce sources. The serotype, virulence, and genotypic resistance profile were determined using the assembled genome sequences sequenced by Illumina technology. Broth microdilution was used to determine the antimicrobial susceptibility of each isolate against a panel of fourteen antimicrobials. The prevalence of E. coli increased during the summer season for all sources tested. ParSNP trees generated demonstrated that the transmission of AMR E. coli is occurring between animal feeding operations and fresh produce. Ten isolates were identified as serotype O45, a serotype that is associated with the “Big Six” group that is frequently linked with foodborne outbreaks caused by non-O157 E. coli. However, these isolates did not possess the stx gene. The highest frequency of resistance was detected against streptomycin (n = 225), ampicillin (n = 190) and sulfisoxazole FIS (n = 140). A total of 35 (13.7%) isolates from two TN farms were positive for the blaCMY (n = 5) and blaTEM (n = 32) genes. The results of this study show the potential of AMR E. coli transmission between animal feeding operations and fresh produce, and more studies are recommended to study this interaction and prevent dissemination in sustainable farming systems.

ACS Style

Ayanna Glaize; Eduardo Gutierrez-Rodriguez; Irene Hanning; Sandra Díaz-Sánchez; Chris Gunter; Arnoud H.M. van Vliet; Wes Watson; Siddhartha Thakur. Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments. International Journal of Food Microbiology 2019, 319, 108472 .

AMA Style

Ayanna Glaize, Eduardo Gutierrez-Rodriguez, Irene Hanning, Sandra Díaz-Sánchez, Chris Gunter, Arnoud H.M. van Vliet, Wes Watson, Siddhartha Thakur. Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments. International Journal of Food Microbiology. 2019; 319 ():108472.

Chicago/Turabian Style

Ayanna Glaize; Eduardo Gutierrez-Rodriguez; Irene Hanning; Sandra Díaz-Sánchez; Chris Gunter; Arnoud H.M. van Vliet; Wes Watson; Siddhartha Thakur. 2019. "Transmission of antimicrobial resistant non-O157 Escherichia coli at the interface of animal-fresh produce in sustainable farming environments." International Journal of Food Microbiology 319, no. : 108472.

Case report
Published: 08 November 2019 in Enfermedades infecciosas y microbiologia clinica (English ed.)
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ACS Style

Mónica De Frutos; Raquel Ramiro; José Isidoro Pereña; Sergio Sánchez. Mixed infection of four diarrhoeagenic Escherichia coli pathotypes in a case of travellers’ diarrhoea: Characterisation of the isolates by whole-genome sequencing. Enfermedades infecciosas y microbiologia clinica (English ed.) 2019, 38, 39 -40.

AMA Style

Mónica De Frutos, Raquel Ramiro, José Isidoro Pereña, Sergio Sánchez. Mixed infection of four diarrhoeagenic Escherichia coli pathotypes in a case of travellers’ diarrhoea: Characterisation of the isolates by whole-genome sequencing. Enfermedades infecciosas y microbiologia clinica (English ed.). 2019; 38 (1):39-40.

Chicago/Turabian Style

Mónica De Frutos; Raquel Ramiro; José Isidoro Pereña; Sergio Sánchez. 2019. "Mixed infection of four diarrhoeagenic Escherichia coli pathotypes in a case of travellers’ diarrhoea: Characterisation of the isolates by whole-genome sequencing." Enfermedades infecciosas y microbiologia clinica (English ed.) 38, no. 1: 39-40.

Review
Published: 01 September 2019 in Trends in Parasitology
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Recently, our knowledge of the composition and complexity of tick microbial communities has increased and supports microbial impact on tick biology. Results support a phylogenetic association between ticks and their microbiota across evolution; this is known as phylosymbiosis. Herein, using published datasets, we confirm the existence of phylosymbiosis between Ixodes ticks and their microbial communities. The strong phylosymbiotic signal and the phylogenetic structure of microbial communities associated with Ixodid ticks revealed that phylosymbiosis may be a widespread phenomenon in tick-microbiota evolution. This finding supports the existence of a species-specific tick hologenome with a largely unexplored influence on tick biology and pathogen transmission. These results may provide potential targets for the construction of paratransgenic ticks to control tick infestations and tick-borne diseases.

ACS Style

Sandra Díaz-Sánchez; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; José de la Fuente. Evolutionary Insights into the Tick Hologenome. Trends in Parasitology 2019, 35, 725 -737.

AMA Style

Sandra Díaz-Sánchez, Agustín Estrada-Peña, Alejandro Cabezas-Cruz, José de la Fuente. Evolutionary Insights into the Tick Hologenome. Trends in Parasitology. 2019; 35 (9):725-737.

Chicago/Turabian Style

Sandra Díaz-Sánchez; Agustín Estrada-Peña; Alejandro Cabezas-Cruz; José de la Fuente. 2019. "Evolutionary Insights into the Tick Hologenome." Trends in Parasitology 35, no. 9: 725-737.

Articles
Published: 19 July 2019 in Avian Pathology
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The poultry red mite, Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of pathogens. However, little is known about the role of poultry red mite in the transmission of pathogenic bacteria as a mechanical carrier or a biological vector. Herein, we used a metaproteomics approach to characterize the alphaproteobacteria in the microbiota of poultry red mite, and variations in its profile with ectoparasite development (nymphs vs. adults) and feeding (unfed vs. fed). The results showed that the bacterial community associated with D. gallinae was mainly composed of environmental and commensal bacteria. Putative symbiotic bacteria of the genera Wolbachia, C. Tokpelaia and Sphingomonas were identified, together with potential pathogenic bacteria of the genera Inquilinus, Neorickettsia and Roseomonas. Significant differences in the composition of alphaproteobacterial microbiota were associated with mite development and feeding, suggesting that bacteria have functional implications in metabolic pathways associated with blood feeding. These results support the use of metaproteomics for the characterization of alphaproteobacteria associated with the D. gallinae microbiota that could provide relevant information for the understanding of mite-host interactions and the development of potential control interventions. Research Highlights - Metaproteomics is a valid approach for microbiome characterization in ectoparasites. - Alphaproteobacteria putative bacterial symbionts were identified in D. gallinae. - Mite development and feeding were related to variations in bacterial community. - Potentially pathogenic bacteria were identified in mite microbiota.

ACS Style

José Francisco Lima-Barbero; Sandra Díaz-Sanchez; Olivier Sparagano; Robert D. Finn; José De La Fuente; Margarita Villar. Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778). Avian Pathology 2019, 48, S52 -S59.

AMA Style

José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José De La Fuente, Margarita Villar. Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778). Avian Pathology. 2019; 48 (sup1):S52-S59.

Chicago/Turabian Style

José Francisco Lima-Barbero; Sandra Díaz-Sanchez; Olivier Sparagano; Robert D. Finn; José De La Fuente; Margarita Villar. 2019. "Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778)." Avian Pathology 48, no. sup1: S52-S59.

Research article
Published: 07 May 2019 in PLoS ONE
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Much work has been dedicated to identifying members of the microbial gut community that have potential to augment the growth rate of agricultural animals including chickens. Here, we assessed any correlations between the fecal microbiome, a proxy for the gut microbiome, and feed efficiency or weight gain at the pedigree chicken level, the highest tier of the production process. Because selective breeding is conducted at the pedigree level, our aim was to determine if microbiome profiles could be used to predict feed conversion or weight gain in order to improve selective breeding. Using 16s rRNA amplicon sequencing, we profiled the microbiomes of high and low weight gain (WG) birds and good and poor feed efficient (FE) birds in two pedigree lineages of broiler chickens. We also aimed to understand the dynamics of the microbiome with respect to maturation. A time series experiment was conducted, where fecal samples of chickens were collected at 6 points of the rearing process and the microbiome of these samples profiled. We identified OTUs differences at different taxonomic levels in the fecal community between high and low performing birds within each genetic line, indicating a specificity of the microbial community profiles correlated to performance factors. Using machine-learning methods, we built a classification model that could predict feed conversion performance from the fecal microbial community. With respect to maturation, we found that the fecal microbiome is dynamic in early life but stabilizes after 3 weeks of age independent of lineage. Our results indicate that the fecal microbiome profile can be used to predict feed conversion, but not weight gain in these pedigree lines. From the time series experiments, it appears that these predictions can be evaluated as early as 20 days of age. Our data also indicates that there is a genetic factor for the microbiome profile.

ACS Style

Sandra Diaz Sanchez; Allison R. Perrotta; Isaac Rockafellow; Eric J. Alm; Ron Okimoto; Rachel Hawken; Irene Hanning. Using fecal microbiota as biomarkers for predictions of performance in the selective breeding process of pedigree broiler breeders. PLoS ONE 2019, 14, e0216080 .

AMA Style

Sandra Diaz Sanchez, Allison R. Perrotta, Isaac Rockafellow, Eric J. Alm, Ron Okimoto, Rachel Hawken, Irene Hanning. Using fecal microbiota as biomarkers for predictions of performance in the selective breeding process of pedigree broiler breeders. PLoS ONE. 2019; 14 (5):e0216080.

Chicago/Turabian Style

Sandra Diaz Sanchez; Allison R. Perrotta; Isaac Rockafellow; Eric J. Alm; Ron Okimoto; Rachel Hawken; Irene Hanning. 2019. "Using fecal microbiota as biomarkers for predictions of performance in the selective breeding process of pedigree broiler breeders." PLoS ONE 14, no. 5: e0216080.

Case report
Published: 11 February 2019 in Enfermedades Infecciosas y Microbiología Clínica
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ACS Style

Mónica De Frutos; Raquel Ramiro; José Isidoro Pereña; Sergio Sánchez. Infección mixta por cuatro patotipos diarreagénicos de Escherichia coli en un caso de diarrea del viajero: caracterización de los aislados obtenidos mediante secuenciación del genoma completo. Enfermedades Infecciosas y Microbiología Clínica 2019, 38, 39 -40.

AMA Style

Mónica De Frutos, Raquel Ramiro, José Isidoro Pereña, Sergio Sánchez. Infección mixta por cuatro patotipos diarreagénicos de Escherichia coli en un caso de diarrea del viajero: caracterización de los aislados obtenidos mediante secuenciación del genoma completo. Enfermedades Infecciosas y Microbiología Clínica. 2019; 38 (1):39-40.

Chicago/Turabian Style

Mónica De Frutos; Raquel Ramiro; José Isidoro Pereña; Sergio Sánchez. 2019. "Infección mixta por cuatro patotipos diarreagénicos de Escherichia coli en un caso de diarrea del viajero: caracterización de los aislados obtenidos mediante secuenciación del genoma completo." Enfermedades Infecciosas y Microbiología Clínica 38, no. 1: 39-40.

Journal article
Published: 19 November 2018 in Ticks and Tick-borne Diseases
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Exploring the microbial diversity of ticks is crucial to understand geographical dispersion and pathogen transmission. Tick microbes participate in many biological processes implicated in the acquisition, maintenance, and transmission of pathogens, and actively promote host phenotypic changes, and adaptation to new environments. The microbial community of Ixodes ventalloi still remains unexplored. In this study, the bacterial microbiota of wild-caught I. ventalloi was characterized using shotgun-metagenomic sequencing in samples from unfed adults collected during December 2013-January 2014 in two locations from Sicily, Italy. The microbiota identified in I. ventalloi was mainly composed of symbiotic, commensal, and environmental bacteria. Interestingly, we identified the genera Anaplasma and Borrelia as members of the microbiota of I. ventalloi. These results advance our information on I. ventalloi microbiota composition, with potential implications in tick-host adaptation, geographic expansion, and vector competence.

ACS Style

Sandra Díaz-Sánchez; Angélica Hernández-Jarguín; Alessandra Torina; Isabel G. Fernández de Mera; Valeria Blanda; Santo Caracappa; Christian Gortazar; José de la Fuente. Characterization of the bacterial microbiota in wild-caught Ixodes ventalloi. Ticks and Tick-borne Diseases 2018, 10, 336 -343.

AMA Style

Sandra Díaz-Sánchez, Angélica Hernández-Jarguín, Alessandra Torina, Isabel G. Fernández de Mera, Valeria Blanda, Santo Caracappa, Christian Gortazar, José de la Fuente. Characterization of the bacterial microbiota in wild-caught Ixodes ventalloi. Ticks and Tick-borne Diseases. 2018; 10 (2):336-343.

Chicago/Turabian Style

Sandra Díaz-Sánchez; Angélica Hernández-Jarguín; Alessandra Torina; Isabel G. Fernández de Mera; Valeria Blanda; Santo Caracappa; Christian Gortazar; José de la Fuente. 2018. "Characterization of the bacterial microbiota in wild-caught Ixodes ventalloi." Ticks and Tick-borne Diseases 10, no. 2: 336-343.

Comparative study
Published: 30 July 2018 in Insect Molecular Biology
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Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun‐metagenomic sequencing of whole‐body DNA samples. Wild‐caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas, Escherichia, Halomonas, Candidatus Zinderia, Propionibacterium, and Schizosaccharomyces. Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences (H. sapiens) and local soil moisture factors shape the microbiome composition of wild‐caught populations of C. imicola. These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector‐borne diseases.

ACS Style

S. Díaz-Sánchez; A. Hernández-Jarguín; A. Torina; I. G. Fernández De Mera; A. Estrada-Peña; M. Villar; F. La Russa; V. Blanda; J. Vicente; S. Caracappa; C. Gortazar; J. De La Fuente. Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola. Insect Molecular Biology 2018, 27, 847 -861.

AMA Style

S. Díaz-Sánchez, A. Hernández-Jarguín, A. Torina, I. G. Fernández De Mera, A. Estrada-Peña, M. Villar, F. La Russa, V. Blanda, J. Vicente, S. Caracappa, C. Gortazar, J. De La Fuente. Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola. Insect Molecular Biology. 2018; 27 (6):847-861.

Chicago/Turabian Style

S. Díaz-Sánchez; A. Hernández-Jarguín; A. Torina; I. G. Fernández De Mera; A. Estrada-Peña; M. Villar; F. La Russa; V. Blanda; J. Vicente; S. Caracappa; C. Gortazar; J. De La Fuente. 2018. "Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola." Insect Molecular Biology 27, no. 6: 847-861.

Journal article
Published: 05 May 2018 in Ticks and Tick-borne Diseases
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An innovative metaomics approach integrating metatranscriptomics and metaproteomics was used to characterize bacterial communities in the microbiota of the Lyme borreliosis spirochete vector, Ixodes ricinus (Acari: Ixodidae). Whole internal tissues and salivary glands from unfed larvae and female ticks, respectively were used. Reused I. ricinus RNA-sequencing data for metranscriptomics analysis together with metaproteomics provided a better characterization of tick bacterial microbiota by increasing bacteria identification and support for identified bacteria with putative functional implications. The results showed the presence of symbiotic, commensal, soil, environmental, and pathogenic bacteria in the I. ricinus microbiota, including previously unrecognized commensal and soil microorganisms. The results of the metaomics approach may have implications in the characterization of putative mechanisms by which pathogen infection manipulates tick microbiota to facilitate infection. Metaomics approaches integrating different omics datasets would provide a better description of tick microbiota compositions, and insights into tick interactions with microbiota, pathogens and hosts.

ACS Style

Angélica Hernández-Jarguín; Sandra Díaz-Sánchez; Margarita Villar; José de la Fuente. Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus. Ticks and Tick-borne Diseases 2018, 9, 1241 -1251.

AMA Style

Angélica Hernández-Jarguín, Sandra Díaz-Sánchez, Margarita Villar, José de la Fuente. Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus. Ticks and Tick-borne Diseases. 2018; 9 (5):1241-1251.

Chicago/Turabian Style

Angélica Hernández-Jarguín; Sandra Díaz-Sánchez; Margarita Villar; José de la Fuente. 2018. "Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus." Ticks and Tick-borne Diseases 9, no. 5: 1241-1251.

Journal article
Published: 01 February 2018 in Genome Announcements
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Here, we report the draft genome sequences of isolates of Anaplasma phagocytophilum , Anaplasma marginale , and Anaplasma ovis . The genomes of A. phagocytophilum (human), A. marginale (cattle), and A. ovis (goat) isolates from the United States were sequenced and characterized. This is the first report of an A. ovis genome sequence.

ACS Style

Sandra Diaz-Sanchez; Angélica Hernández-Jarguín; Isabel G. Fernández de Mera; Pilar Alberdi; Erich Zweygarth; Christian Gortazar; José de la Fuente. Draft Genome Sequences of Anaplasma phagocytophilum , A. marginale , and A. ovis Isolates from Different Hosts. Genome Announcements 2018, 6, 1 .

AMA Style

Sandra Diaz-Sanchez, Angélica Hernández-Jarguín, Isabel G. Fernández de Mera, Pilar Alberdi, Erich Zweygarth, Christian Gortazar, José de la Fuente. Draft Genome Sequences of Anaplasma phagocytophilum , A. marginale , and A. ovis Isolates from Different Hosts. Genome Announcements. 2018; 6 (5):1.

Chicago/Turabian Style

Sandra Diaz-Sanchez; Angélica Hernández-Jarguín; Isabel G. Fernández de Mera; Pilar Alberdi; Erich Zweygarth; Christian Gortazar; José de la Fuente. 2018. "Draft Genome Sequences of Anaplasma phagocytophilum , A. marginale , and A. ovis Isolates from Different Hosts." Genome Announcements 6, no. 5: 1.

Journal article
Published: 01 August 2017 in Emerging Infectious Diseases
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We identified the mucus-activatable Shiga toxin genotype stx2d in the most common hemolytic uremic syndrome–associated Escherichia coli serotype, O157:H7. stx2d was detected in a strain isolated from a 2-year-old boy with bloody diarrhea in Spain, and whole-genome sequencing was used to confirm and fully characterize the strain.

ACS Style

Sergio Sánchez; María Teresa Llorente; Laura Herrera-León; Raquel Ramiro; Sandra Nebreda; María Antonia Remacha; Silvia Herrera-León. Mucus-Activatable Shiga Toxin Genotypestx2dinEscherichia coliO157:H7. Emerging Infectious Diseases 2017, 23, 1431 -1433.

AMA Style

Sergio Sánchez, María Teresa Llorente, Laura Herrera-León, Raquel Ramiro, Sandra Nebreda, María Antonia Remacha, Silvia Herrera-León. Mucus-Activatable Shiga Toxin Genotypestx2dinEscherichia coliO157:H7. Emerging Infectious Diseases. 2017; 23 (8):1431-1433.

Chicago/Turabian Style

Sergio Sánchez; María Teresa Llorente; Laura Herrera-León; Raquel Ramiro; Sandra Nebreda; María Antonia Remacha; Silvia Herrera-León. 2017. "Mucus-Activatable Shiga Toxin Genotypestx2dinEscherichia coliO157:H7." Emerging Infectious Diseases 23, no. 8: 1431-1433.

Journal article
Published: 01 June 2017 in Poultry Science
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When prebiotics and other fermentation substrates are delivered to animals as feed supplements, the typical goal is to improve weight gain and feed conversion. In this work, we examined pasture flock chicken cecal contents using next generation sequencing (NGS) to identify and understand the composition of the microbiome when prebiotics and fermentation substrates were supplemented. We generated 16S rRNA sequencing data for 120 separate cecal samples from groups of chickens receiving one of 3 prebiotics or fiber feed additives. The data indicated that respective feed additives enrich for specific bacterial community members and modulate the diversity of the microbiome. We applied synthetic learning in microbial ecology (SLiME) analysis to interpret 16S rRNA microbial community data and identify specific bacterial operational taxonomic units (OTU) that are predictive of the particular feed additives used in these experiments. The results suggest that feed can influence microbiome composition in a predictable way, and thus diet may have indirect effects on weight gain and feed conversion through the microbiome.

ACS Style

S. H. Park; A. Perrotta; I. Hanning; Sandra Diaz Sanchez; S. Pendleton; E. Alm; S. C. Ricke. Pasture flock chicken cecal microbiome responses to prebiotics and plum fiber feed amendments. Poultry Science 2017, 96, 1820 -1830.

AMA Style

S. H. Park, A. Perrotta, I. Hanning, Sandra Diaz Sanchez, S. Pendleton, E. Alm, S. C. Ricke. Pasture flock chicken cecal microbiome responses to prebiotics and plum fiber feed amendments. Poultry Science. 2017; 96 (6):1820-1830.

Chicago/Turabian Style

S. H. Park; A. Perrotta; I. Hanning; Sandra Diaz Sanchez; S. Pendleton; E. Alm; S. C. Ricke. 2017. "Pasture flock chicken cecal microbiome responses to prebiotics and plum fiber feed amendments." Poultry Science 96, no. 6: 1820-1830.

Book chapter
Published: 31 December 2016 in Achieving sustainable production of eggs Volume 1
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The zoonotic pathogen, Campylobacter, is the leading cause of foodborne illness worldwide. It colonizes the intestinal tract of chickens, its primary host. In chickens, this pathogen is considered commensal and does not cause illness or have adverse impacts on production performance. A number of methods aim at controlling Campylobacter in the farm. This chapter discusses the general features and epidemiology of Campylobacter, improving biosecurity in the farm, antimicrobial therapy and increased host resistance, boosting the immune response, and DNA vaccines for preventing campylobacteriosis. It is concluded that, along with biosecurity, preventative treatments for the birds can act synergistically to significantly reduce the risk of contamination.

ACS Style

Xiang Liu; Irene Hanning; Sandra Diaz-Sanchez; Jun Lin; Spain SaBio IREC. Food safety control on poultry farms: effective control of Campylobacter. Achieving sustainable production of eggs Volume 1 2016, 193 -206.

AMA Style

Xiang Liu, Irene Hanning, Sandra Diaz-Sanchez, Jun Lin, Spain SaBio IREC. Food safety control on poultry farms: effective control of Campylobacter. Achieving sustainable production of eggs Volume 1. 2016; ():193-206.

Chicago/Turabian Style

Xiang Liu; Irene Hanning; Sandra Diaz-Sanchez; Jun Lin; Spain SaBio IREC. 2016. "Food safety control on poultry farms: effective control of Campylobacter." Achieving sustainable production of eggs Volume 1 , no. : 193-206.

Review
Published: 10 November 2015 in Microbiome
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Due to the significance of the microbiome on human health, much of the current data available regarding microbiome functionality is centered on human medicine. For agriculturally important taxa, the functionality of gastrointestinal bacteria has been studied with the primary goals of improving animal health and production performance. With respect to cattle, the digestive functions of bacteria in cattle are unarguably critical to digestion and positively impact production performance. Conversely, some research suggests that the gastrointestinal microbiome in chickens competes with the host for nutrients and produces toxins that can harm the host resulting in decreased growth efficiency. Concerning many other species including reptiles and cetaceans, some cataloging of fecal bacteria has been conducted, but the functionality within the host remains ambiguous. These taxa could provide interesting gastrointestinal insight into functionality and symbiosis considering the extreme feeding regimes (snakes), highly specialized diets (vampire bats), and living environments (polar bears), which warrants further exploration.

ACS Style

Irene Hanning; Sandra Díaz-Sánchez. The functionality of the gastrointestinal microbiome in non-human animals. Microbiome 2015, 3, 51 .

AMA Style

Irene Hanning, Sandra Díaz-Sánchez. The functionality of the gastrointestinal microbiome in non-human animals. Microbiome. 2015; 3 (1):51.

Chicago/Turabian Style

Irene Hanning; Sandra Díaz-Sánchez. 2015. "The functionality of the gastrointestinal microbiome in non-human animals." Microbiome 3, no. 1: 51.

Review
Published: 01 June 2015 in Poultry Science
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The development of antibiotic resistant pathogens has resulted from the use of sub-therapeutic concentrations of antibiotics delivered in poultry feed. Furthermore, there are a number of consumer concerns regarding the use of antibiotics in food animals including residue contamination of poultry products and antibiotic resistant bacterial pathogens. These issues have resulted in recommendations to reduce the use of antibiotics as growth promoters in livestock in the United States. Unlike conventional production, organic systems are not permitted to use antibiotics. Thus, both conventional and organic poultry production need alternative methods to improve growth and performance of poultry. Herbs, spices, and various other plant extracts are being evaluated as alternatives to antibiotics and some do have growth promoting effects, antimicrobial properties, and other health-related benefits. This review aims to provide an overview of herbs, spices, and plant extracts, currently defined as phytobiotics as potential feed additives.

ACS Style

Sandra Diaz-Sanchez; Doris D'Souza; Debrabrata Biswas; Irene Hanning. Botanical alternatives to antibiotics for use in organic poultry production. Poultry Science 2015, 94, 1419 -1430.

AMA Style

Sandra Diaz-Sanchez, Doris D'Souza, Debrabrata Biswas, Irene Hanning. Botanical alternatives to antibiotics for use in organic poultry production. Poultry Science. 2015; 94 (6):1419-1430.

Chicago/Turabian Style

Sandra Diaz-Sanchez; Doris D'Souza; Debrabrata Biswas; Irene Hanning. 2015. "Botanical alternatives to antibiotics for use in organic poultry production." Poultry Science 94, no. 6: 1419-1430.

Research article
Published: 28 January 2015 in PLoS ONE
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Escherichia coli serogroups O5, O15, O26, O45, O55, O76, O91, O103, O104, O111, O113, O118, O121, O123, O128, O145, O146, O157, O165, O172, and O177 are the O-antigen forms of the most clinically relevant Shiga toxin-producing E. coli (STEC) serotypes. In this study, three multiplex PCR assays able to specifically detect these 21 serogroups were developed and validated. For this purpose, the O-antigen gene clusters of E. coli O5 and O76 were fully sequenced, their associated genes were identified on the basis of homology, and serogroup-specific primers were designed. After preliminary evaluation, these two primer pairs were proven to be highly specific and suitable for the development of PCR assays for O5 and O76 serogroup identification. Specific primers were also designed for serogroups O15, O45, O55, O91, O104, O113, O118, O123, O128, O146, O157, O165, O172, and O177 based on previously published sequences, and previously published specific primers for serogroups O26, O103, O111, O121, and O145 were also included. These 21 primer pairs were shown to be specific for their target serogroup when tested against E. coli type strains representing 169 known O-antigen forms of E. coli and Shigella and therefore suitable for being used in PCR assays for serogroup identification. In order to validate the three multiplex PCR assays, 22 E. coli strains belonging to the 21 covered serogroups and 18 E. coli strains belonging to other serogroups were screened in a double-blind test and their sensitivity was determined as 1 ng chromosomal DNA. The PCR assays developed in this study could be a faster, simpler, and less expensive strategy for serotyping of the most clinically relevant STEC strains in both clinical microbiology and public health laboratories, and so their development could benefit for clinical diagnosis, epidemiological investigations, surveillance, and control of STEC infections.

ACS Style

Sergio Sánchez; María Teresa Llorente; María Aurora Echeita; Silvia Herrera-León. Development of Three Multiplex PCR Assays Targeting the 21 Most Clinically Relevant Serogroups Associated with Shiga Toxin-Producing E. coli Infection in Humans. PLoS ONE 2015, 10, e0117660 -e0117660.

AMA Style

Sergio Sánchez, María Teresa Llorente, María Aurora Echeita, Silvia Herrera-León. Development of Three Multiplex PCR Assays Targeting the 21 Most Clinically Relevant Serogroups Associated with Shiga Toxin-Producing E. coli Infection in Humans. PLoS ONE. 2015; 10 (1):e0117660-e0117660.

Chicago/Turabian Style

Sergio Sánchez; María Teresa Llorente; María Aurora Echeita; Silvia Herrera-León. 2015. "Development of Three Multiplex PCR Assays Targeting the 21 Most Clinically Relevant Serogroups Associated with Shiga Toxin-Producing E. coli Infection in Humans." PLoS ONE 10, no. 1: e0117660-e0117660.