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Dr. Hyun-Jae Shin
Dept Biochem & Polymer Engineering, Chosun University

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0 Biotechnology
0 Fermentation Technology
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Short Biography

Hyun-Jae Shin is a Professor of Biochemical Engineering at Chosun University, Gwangju, Korea. He studied chemical engineering at Inha University and obtained a PhD in biochemical engineering at Korea Advanced Institute of Science and Technology (KAIST) (1996). Completed postdoctoral work at University of Westminster (as a Chevening Scholarship recipient of British Council). After working three years in Korea Research Institute of Bioscience and Biotechnology (1998-2000), he has established his own start-up biotech, EnzBank, Inc., which has run for six years. He started his lab at Chosun University (2006) and is responsible for many national and industrial research projects. Hyun-Jae Shin has published over 100 papers (Google scholar citation 1819 and h-index 24 as of January 2018), 20 patents either registered or filed, and 6 books published. Hyun-Jae Shin served as Editor-in-Chief, Korea Society for Biotechnology and Bioengineering Journal and Journal of Mushrooms. His research interests include enzyme technology, nanotechnology, environmental engineering and utilization of natural resources.

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Review
Published: 24 June 2021 in Cosmetics
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Many recent studies in the field of cosmetics have focused on organically sourced substances. Products made from organic materials are safe, high quality, cruelty-free, and more effective than those made from synthetic materials. Many organic compounds are known to be physiologically active in humans and have an extended storage capacity and long-lasting environmental effects. Agro-industrial waste has recently increased substantially, and the disposal of date palm waste, often performed in primitive ways such as burning, is harmful to the environment. Fruit processing industries generate over 10% of the total date seed waste daily, which could be converted into useful food products. Date fruit and seed are rich in sugar, vitamins, fiber, minerals, and phenolic compounds with antioxidant and anti-inflammatory properties that significantly promote human and animal health. This waste is rich in bioactive compounds and essential oils used in many kinds of food, medicine, and cosmetics. Most active cosmetic ingredients come from natural sources such as fruit, fish, and dairy, and recent research shows that date extract and seed oil help to reduce melanin, eczema, acne, and dry patches, while increasing skin moisture and elasticity. This review details the bioactive compounds and nutraceutical properties of date fruit and seed, and their use as cosmetic ingredients.

ACS Style

Khlood Alharbi; Jegadeesh Raman; Hyun-Jae Shin. Date Fruit and Seed in Nutricosmetics. Cosmetics 2021, 8, 59 .

AMA Style

Khlood Alharbi, Jegadeesh Raman, Hyun-Jae Shin. Date Fruit and Seed in Nutricosmetics. Cosmetics. 2021; 8 (3):59.

Chicago/Turabian Style

Khlood Alharbi; Jegadeesh Raman; Hyun-Jae Shin. 2021. "Date Fruit and Seed in Nutricosmetics." Cosmetics 8, no. 3: 59.

Journal article
Published: 20 April 2021 in International Journal of Molecular Sciences
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Quercetin is a well-known plant flavonol and antioxidant; however, there has been some debate regarding the efficacy and safety of native quercetin as a skin-whitening agent via tyrosinase inhibition. Several researchers have synthesized quercetin derivatives as low-toxicity antioxidants and whitening agents. However, no suitable quercetin derivatives have been reported to date. In this study, a novel quercetin derivative was synthesized by the SN2 reaction using quercetin and oleyl bromide. The relationship between the structures and activities of quercetin derivatives as anti-melanogenic agents was assessed using in vitro enzyme kinetics, molecular docking, and quenching studies; cell line experiments; and in vivo zebrafish model studies. Novel 3,7-dioleylquercetin (OQ) exhibited a low cytotoxic concentration level at >100 µg/mL (125 µM), which is five times less toxic than native quercetin. The inhibition mechanism showed that OQ is a competitive inhibitor, similar to native quercetin. Expression of tyrosinase, tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor was inhibited in B16F10 melanoma cell lines. mRNA transcription levels of tyrosinase, TRP-1, and TRP-2 decreased in a dose-dependent manner. Melanin formation was confirmed in the zebrafish model using quercetin derivatives. Therefore, OQ might be a valuable asset for the development of novel skin-whitening agents.

ACS Style

Moon-Hee Choi; Seung-Hwa Yang; Da-Song Kim; Nam Kim; Hyun-Jae Shin; Kechun Liu. Novel Quercetin Derivative of 3,7-Dioleylquercetin Shows Less Toxicity and Highly Potent Tyrosinase Inhibition Activity. International Journal of Molecular Sciences 2021, 22, 4264 .

AMA Style

Moon-Hee Choi, Seung-Hwa Yang, Da-Song Kim, Nam Kim, Hyun-Jae Shin, Kechun Liu. Novel Quercetin Derivative of 3,7-Dioleylquercetin Shows Less Toxicity and Highly Potent Tyrosinase Inhibition Activity. International Journal of Molecular Sciences. 2021; 22 (8):4264.

Chicago/Turabian Style

Moon-Hee Choi; Seung-Hwa Yang; Da-Song Kim; Nam Kim; Hyun-Jae Shin; Kechun Liu. 2021. "Novel Quercetin Derivative of 3,7-Dioleylquercetin Shows Less Toxicity and Highly Potent Tyrosinase Inhibition Activity." International Journal of Molecular Sciences 22, no. 8: 4264.

Review paper
Published: 10 February 2021 in Biotechnology and Bioprocess Engineering
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Viral nanoparticles (VNPs) comprise a variety of mammalian viruses, plant viruses, and bacteriophages, that have been adopted as building blocks and supra-molecular templates in nanotechnology. VNPs demonstrate the dynamic, monodisperse, polyvalent, and symmetrical architectures which represent examples of such biological templates. These programmable scaffolds have been exploited for genetic and chemical manipulation for displaying of targeted moieties together with encapsulation of various payloads for diagnosis or therapeutic intervention. The drug delivery system based on VNPs offer diverse advantages over synthetic nanoparticles, including biocompatibility, biodegradability, water solubility, and high uptake capability. Here we summarize the recent progress of VNPs especially as targeted anticancer vehicles from the encapsulation and surface modification mechanisms, involved viruses and VNPs, to their application potentials.

ACS Style

Yuanzheng Wu; Jishun Li; Hyun-Jae Shin. Self-assembled Viral Nanoparticles as Targeted Anticancer Vehicles. Biotechnology and Bioprocess Engineering 2021, 26, 25 -38.

AMA Style

Yuanzheng Wu, Jishun Li, Hyun-Jae Shin. Self-assembled Viral Nanoparticles as Targeted Anticancer Vehicles. Biotechnology and Bioprocess Engineering. 2021; 26 (1):25-38.

Chicago/Turabian Style

Yuanzheng Wu; Jishun Li; Hyun-Jae Shin. 2021. "Self-assembled Viral Nanoparticles as Targeted Anticancer Vehicles." Biotechnology and Bioprocess Engineering 26, no. 1: 25-38.

Journal article
Published: 06 February 2021 in Agriculture
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Sweet potatoes are a substantial source of nutrition and can be added to processed foods in the form of paste. The moisture and starch contents of these potatoes affect the physicochemical properties of sweet potato paste. In this study, the changes in the moisture, starch, and α-amylase content of sweet potatoes were measured for eight weeks after harvest. Using nondestructive near-infrared analyses and chemometric models, the moisture and starch contents were predicted. The partial least squares (PLS) method was used for prediction, while linear discriminant analysis (LDA) was used for discrimination. To increase the accuracy of the model, the near-infrared spectrum was preprocessed using the Savitzky–Golay derivative (S–G), standard normal variate (SNV), and multiplicative scattering correction methods. When applying PLS to the moisture content, the best calibration model accuracy was obtained using the S–G preprocessed spectrum. Furthermore, the best calibration model accuracy for starch content was obtained using the SNV preprocessed spectrum. The moisture and starch contents were categorized into five classes for LDA, with results indicating that the internal quality of sweet potatoes can be predicted and classified using chemometric models through nondestructive detection.

ACS Style

Da-Song Kim; Moon-Hee Choi; Hyun-Jae Shin. Estimation of Starch Hydrolysis in Sweet Potato (Beni Haruka) Based on Storage Period Using Nondestructive Near-Infrared Spectrometry. Agriculture 2021, 11, 135 .

AMA Style

Da-Song Kim, Moon-Hee Choi, Hyun-Jae Shin. Estimation of Starch Hydrolysis in Sweet Potato (Beni Haruka) Based on Storage Period Using Nondestructive Near-Infrared Spectrometry. Agriculture. 2021; 11 (2):135.

Chicago/Turabian Style

Da-Song Kim; Moon-Hee Choi; Hyun-Jae Shin. 2021. "Estimation of Starch Hydrolysis in Sweet Potato (Beni Haruka) Based on Storage Period Using Nondestructive Near-Infrared Spectrometry." Agriculture 11, no. 2: 135.

Research paper
Published: 30 January 2021 in Biotechnology and Bioprocess Engineering
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As a nutritional supplement, wheatgrass (Triticum aestivum L.) is well-known for its anti-inflammatory effects that are exerted through signal transduction pathways. Wheatgrass was extracted in 80% ethanol (WGE), and the ethanol extracts were further fractionated using ethyl acetate, n-butanol, and water. To determine the antioxidant activity, WGE was tested using various radical-scavenging assays. To explain the anti-inflammatory mechanisms, western blotting, reverse-transcription polymerase chain reaction, luciferase assay, immunofluorescence staining, and the inhibition of mitogen-activated protein kinase (MAPK) phosphorylation were performed using mouse macrophage cells (RAW 264.7). Inhibition of proinflammatory and Th2 cytokines was analyzed using a 2,4-dinitrochlorobenzene-induced contact dermatitis mouse model. WGE exhibited a strong antioxidant activity in radical scavenging-assays conducted using artificial and natural substrates. The ethyl acetate and n-butanol fractions (hydrophobic fractions) obtained from the 80% ethanolic extract, inhibited the expression of inducible nitric oxide synthase gene, whereas the aqueous layer (the hydrophilic fraction) showed no inhibitory effects. In addition, the hydrophobic fractions inhibited nuclear factor-kappa B (NF-κB) and reduced phosphorylation of MAPK, indicating that inflammation was alleviated possibly by the regulation of NF-κB signaling. This mechanism was further elucidated using the RAW 264.7 cell line and mouse model. Using high-performance liquid chromatography, the hydrophobic fractions were separated into over 10 phenolic compounds, among which benzoic acid, quercetin, and luteolin were abundant. The study demonstrated that wheatgrass is a potential nutraceutical candidate with antioxidant and anti-inflammatory properties for future use in health foods and cosmetics.

ACS Style

Moon-Hee Choi; Min Young Lee; Seung-Hwa Yang; Hyun-Jae Shin; Young Jin Jeon. Hydrophobic Fractions of Triticum aestivum L. Extracts Contain Polyphenols and Alleviate Inflammation by Regulating Nuclear Factor-kappa B. Biotechnology and Bioprocess Engineering 2021, 26, 93 -106.

AMA Style

Moon-Hee Choi, Min Young Lee, Seung-Hwa Yang, Hyun-Jae Shin, Young Jin Jeon. Hydrophobic Fractions of Triticum aestivum L. Extracts Contain Polyphenols and Alleviate Inflammation by Regulating Nuclear Factor-kappa B. Biotechnology and Bioprocess Engineering. 2021; 26 (1):93-106.

Chicago/Turabian Style

Moon-Hee Choi; Min Young Lee; Seung-Hwa Yang; Hyun-Jae Shin; Young Jin Jeon. 2021. "Hydrophobic Fractions of Triticum aestivum L. Extracts Contain Polyphenols and Alleviate Inflammation by Regulating Nuclear Factor-kappa B." Biotechnology and Bioprocess Engineering 26, no. 1: 93-106.

Journal article
Published: 25 June 2020 in Journal of Fungi
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This study aimed to investigate the elicitation effects of alginate oligosaccharides extracted from brown algae (Sargassum species) on β-glucan production in cauliflower mushroom (Sparassis latifolia). Sodium alginate was refined from Sargassum fulvellum, S. fusiforme, and S. horneri, and characterized by proton nuclear magnetic resonance spectroscopy (1H NMR), resulting mannuronic acid to guluronic acid (M/G) rationes from 0.64 to 1.38. Three oligosaccharide fractions, ethanol fraction (EF), solid fraction (SF), and liquid fraction (LF), were prepared by acid hydrolysis and analyzed by Fourier transform infrared (FT-IR) spectra and high-performance anion-exchange chromatography with a pulsed amperometric detector (HPAEC-PAD). The samples of S. fusiforme resulted in the highest hydrolysate in SF and the lowest in LF, which was consistent with its highest M/G ratio. The SF of S. fusiforme and LF of S. horneri were chosen for elicitation on S. latifolia, yielding the highest β-glucan contents of 56.01 ± 3.45% and 59.74 ± 4.49% in the stalk, respectively. Total polyphenol content (TPC) and antioxidant activities (2,2’-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging and Superoxide dismutase (SOD)-like activity) of aqueous extracts of S. latifolia were greatly stimulated by alginate elicitation. These results demonstrate that alginate oligosaccharides extracted from brown algae may be useful as an elicitor to enhance the nutritional value of mushrooms.

ACS Style

Yong-Woon Kim; Yuanzheng Wu; Moon-Hee Choi; Hyun-Jae Shin; Jishun Li. Alginate-Derived Elicitors Enhance β-Glucan Content and Antioxidant Activities in Culinary and Medicinal Mushroom, Sparassis latifolia. Journal of Fungi 2020, 6, 92 .

AMA Style

Yong-Woon Kim, Yuanzheng Wu, Moon-Hee Choi, Hyun-Jae Shin, Jishun Li. Alginate-Derived Elicitors Enhance β-Glucan Content and Antioxidant Activities in Culinary and Medicinal Mushroom, Sparassis latifolia. Journal of Fungi. 2020; 6 (2):92.

Chicago/Turabian Style

Yong-Woon Kim; Yuanzheng Wu; Moon-Hee Choi; Hyun-Jae Shin; Jishun Li. 2020. "Alginate-Derived Elicitors Enhance β-Glucan Content and Antioxidant Activities in Culinary and Medicinal Mushroom, Sparassis latifolia." Journal of Fungi 6, no. 2: 92.

Full article
Published: 20 May 2020 in Journal of Food Biochemistry
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Moringa oleifera is rich in nutrients, such as protein, vitamins, and phytochemicals, and has been used as a traditional remedy. In this study, extracts of M. oleifera leaves from South Korea and Cambodia were evaluated for their antioxidant and antiobesity activities and for food and natural medicine use. The extracts were made using water and ethanol with leaves from South Korea and Cambodia, and then, the ethanol extracts were further fractionated with ethyl acetate. The antioxidant and antiobesity activities of fractionated ethanol extracts were higher than those of water extracts. Although the expression of C/EBPα in 3T3‐L1 cell differentiation did not have a concentration‐dependent inhibitory effect on the M. oleifera leaf extracts, the expression of PPARγ, FAS, and ACC was inhibited in a concentration‐dependent manner with the M. oleifera leaf extracts. This study shows that M. oleifera leaves from South Korea and Cambodia may be an effective candidate for antiobesity prevention. Practical applications Antioxidants and antiobesity factors are important for metabolic syndrome including obesity. Recently, natural antiobesity medication containing polyphenol ingredients has been developed to replace synthetic antiobesity medication, which has various side effects. This study evaluates the antioxidant and antiobesity activities of Moringa oleifera leaves from different cultivation regions. The leaves grow rapidly and leaf extracts contain a large amount of nutrients and phytochemicals, which enables commercial production of the leaves as natural antiobesity medications including functional foods and nutraceuticals.

ACS Style

Da‐Song Kim; Moon‐Hee Choi; Hyun‐Jae Shin. Extracts of Moringa oleifera leaves from different cultivation regions show both antioxidant and antiobesity activities. Journal of Food Biochemistry 2020, 44, e13282 .

AMA Style

Da‐Song Kim, Moon‐Hee Choi, Hyun‐Jae Shin. Extracts of Moringa oleifera leaves from different cultivation regions show both antioxidant and antiobesity activities. Journal of Food Biochemistry. 2020; 44 (7):e13282.

Chicago/Turabian Style

Da‐Song Kim; Moon‐Hee Choi; Hyun‐Jae Shin. 2020. "Extracts of Moringa oleifera leaves from different cultivation regions show both antioxidant and antiobesity activities." Journal of Food Biochemistry 44, no. 7: e13282.

Journal article
Published: 08 January 2019 in Nutrients
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This study was designed to investigate the hepatoprotective effect of bamboo stems using in vitro and in vivo experimental liver damage models. Ethyl acetate fraction of 80% ethanol extract of Phyllostachys nigra stem (PN3) containing polyphenols had a higher NQO1-ARE reporter gene activity as monitored by the activity of the NF-E2-related factor (Nrf2) antioxidant pathway in cells in comparison to extracts from other species and under other conditions. The Nrf2 was translocated from the cytosol to the nucleus in response to PN3, followed by induction of the Nrf2 target gene expression, including HO-1, GCL, and NQO-1 in HepG2 cells. Phosphorylation of Nrf2 in HepG2 cells was enhanced in PN3, which was mediated by PKCδ, ERK, and p38 MAPK. Consequently, PN3 inhibited arachidonic acid (AA) + iron-induced reactive oxygen species generation and glutathione depletion, and, thus, highlighted their role in cytotoxicity. Treatment with major polyphenols of PN3, including catechin, chlorogenic acid, caffeic acid, and p-coumaric acid, also improved AA + iron-mediated oxidative stress and, thus, improved cell viability. Treatment with phenylhydrazine in mice, i.e., the iron overload liver injury model, increased plasma alanine aminotransferase and aspartate aminotransferase levels and changed histological features in mice—a response that was almost completely blocked by PN3 administration. Moreover, PN3 extract mitigated phenylhydrazine-induced oxidative stress and inflammatory responses. Conclusively, PN3 can exert a hepatoprotective effect against iron overload-induced acute liver damage due to its antioxidant properties.

ACS Style

Ji Hye Yang; Moon-Hee Choi; Chang-Su Na; Sam Seok Cho; Jae Hoon Kim; Sae Kwang Ku; Il Je Cho; Hyun-Jae Shin; Sung Hwan Ki. Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients 2019, 11, 114 .

AMA Style

Ji Hye Yang, Moon-Hee Choi, Chang-Su Na, Sam Seok Cho, Jae Hoon Kim, Sae Kwang Ku, Il Je Cho, Hyun-Jae Shin, Sung Hwan Ki. Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients. 2019; 11 (1):114.

Chicago/Turabian Style

Ji Hye Yang; Moon-Hee Choi; Chang-Su Na; Sam Seok Cho; Jae Hoon Kim; Sae Kwang Ku; Il Je Cho; Hyun-Jae Shin; Sung Hwan Ki. 2019. "Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury." Nutrients 11, no. 1: 114.

Research papers
Published: 13 December 2018 in Korean Journal of Chemical Engineering
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Dye wastewater causing destruction in ecosystem from a variety of plants an operation needs various factors for environmental cleanup. To improve removal efficiency of dye wastewater, various adsorbents including clay and nonclay-related materials have been tried. The use of soluble aminopropyl functionalized magnesium phyllosilicate (Mg-AMP clay) as an adsorbent for the textile anionic dye Reactive Red 120 (RR 120) was examined thermodynamically and kinetically. The adsorption kinetics followed the pseudo-second-order and Langmuir isotherm equation fitted best models. A maximum amount of adsorption was determined to be 229.9 mg/g, which is one of the highest values studied so far. An Mg-AMP clay dosage of 10 mg/mL obtained from Langmuir model a maximum adsorption capacity of 229.94, 182.26 and 156.54 mg/g at 298.15, 308.15 and 318.15 K, respectively. Moreover the thermodynamic activation parameters such as enthalpy and entropy were determined. We suggest the removal mechanism of RR 120 using Mg-AMP clay by adsorption and precipitation.

ACS Style

Yong-Woon Kim; Jung-Hun Kim; Deok Hyun Moon; Hyun-Jae Shin. Adsorption and precipitation of anionic dye Reactive Red 120 from aqueous solution by aminopropyl functionalized magnesium phyllosilicate. Korean Journal of Chemical Engineering 2018, 36, 101 -108.

AMA Style

Yong-Woon Kim, Jung-Hun Kim, Deok Hyun Moon, Hyun-Jae Shin. Adsorption and precipitation of anionic dye Reactive Red 120 from aqueous solution by aminopropyl functionalized magnesium phyllosilicate. Korean Journal of Chemical Engineering. 2018; 36 (1):101-108.

Chicago/Turabian Style

Yong-Woon Kim; Jung-Hun Kim; Deok Hyun Moon; Hyun-Jae Shin. 2018. "Adsorption and precipitation of anionic dye Reactive Red 120 from aqueous solution by aminopropyl functionalized magnesium phyllosilicate." Korean Journal of Chemical Engineering 36, no. 1: 101-108.

Journal article
Published: 30 September 2018 in KSBB Journal
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ACS Style

Seung-Hwa Yang; Moon-Hee Choi; Hyun-Jae Shin. Antioxidant Activities of Lignin Extracted from Domestic Bamboo (Phyllostachys nigra var. Henonis) Stem. KSBB Journal 2018, 33, 200 -208.

AMA Style

Seung-Hwa Yang, Moon-Hee Choi, Hyun-Jae Shin. Antioxidant Activities of Lignin Extracted from Domestic Bamboo (Phyllostachys nigra var. Henonis) Stem. KSBB Journal. 2018; 33 (3):200-208.

Chicago/Turabian Style

Seung-Hwa Yang; Moon-Hee Choi; Hyun-Jae Shin. 2018. "Antioxidant Activities of Lignin Extracted from Domestic Bamboo (Phyllostachys nigra var. Henonis) Stem." KSBB Journal 33, no. 3: 200-208.

Journal article
Published: 01 April 2018 in Algal Research
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ACS Style

Se Won Kim; Yong-Woon Kim; Chae-Hwan Hong; In-Woong Lyo; Ho-Dong Lim; Geun-Joong Kim; Hyun-Jae Shin. Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae. Algal Research 2018, 31, 517 -524.

AMA Style

Se Won Kim, Yong-Woon Kim, Chae-Hwan Hong, In-Woong Lyo, Ho-Dong Lim, Geun-Joong Kim, Hyun-Jae Shin. Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae. Algal Research. 2018; 31 ():517-524.

Chicago/Turabian Style

Se Won Kim; Yong-Woon Kim; Chae-Hwan Hong; In-Woong Lyo; Ho-Dong Lim; Geun-Joong Kim; Hyun-Jae Shin. 2018. "Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae." Algal Research 31, no. : 517-524.

Journal article
Published: 31 March 2018 in KSBB Journal
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ACS Style

Moon-Hee Choi; Seung-Hwa Yang; Hyun-Jae Shin. Inhibition of Melanogenesis by Domestic Bamboo Leaves (Sasa coreana Nakai) Extract in B16F10 Melanoma Cells. KSBB Journal 2018, 33, 41 -47.

AMA Style

Moon-Hee Choi, Seung-Hwa Yang, Hyun-Jae Shin. Inhibition of Melanogenesis by Domestic Bamboo Leaves (Sasa coreana Nakai) Extract in B16F10 Melanoma Cells. KSBB Journal. 2018; 33 (1):41-47.

Chicago/Turabian Style

Moon-Hee Choi; Seung-Hwa Yang; Hyun-Jae Shin. 2018. "Inhibition of Melanogenesis by Domestic Bamboo Leaves (Sasa coreana Nakai) Extract in B16F10 Melanoma Cells." KSBB Journal 33, no. 1: 41-47.

Research paper
Published: 01 February 2018 in Biotechnology and Bioprocess Engineering
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Carotenoids, found in many fruits and vegetables, are antioxidants that protect human skin from UV radiation. In humans, fruit and vegetable intake increases carotenoid contents in skin, which are conventionally assessed by invasive blood tests. In this study, 47 healthy Korean subjects (volunteers) consumed fruit juice containing tomato, apple, strawberry, or grape three times per week for 6 weeks. Skin antioxidant levels were measured by non-invasive resonance Raman spectroscopy. The correlation between skin carotenoid (SC) score with demographic data (age, height, weight) and juice supplementation and changes in SC scores among groups were analyzed. Variations in skin antioxidant levels increased with juice supplementation (p < 0.05). Fruit juice intake was significantly correlated with SC score, indicating increased skin antioxidant levels. Grape and tomato increased skin antioxidant levels and showed higher antioxidant activity than other fruits. Fruit juices containing high levels of carotenoids and antioxidants may provide modest benefits to human health.

ACS Style

Moon-Hee Choi; Han-Gyo Jo; Min-Ju Kim; Min-Jung Kang; Hyun-Jae Shin. Fruit Juice Supplementation Alters Human Skin Antioxidant Levels In Vivo: Case Study of Korean Adults by Resonance Raman Spectroscopy. Biotechnology and Bioprocess Engineering 2018, 23, 116 -121.

AMA Style

Moon-Hee Choi, Han-Gyo Jo, Min-Ju Kim, Min-Jung Kang, Hyun-Jae Shin. Fruit Juice Supplementation Alters Human Skin Antioxidant Levels In Vivo: Case Study of Korean Adults by Resonance Raman Spectroscopy. Biotechnology and Bioprocess Engineering. 2018; 23 (1):116-121.

Chicago/Turabian Style

Moon-Hee Choi; Han-Gyo Jo; Min-Ju Kim; Min-Jung Kang; Hyun-Jae Shin. 2018. "Fruit Juice Supplementation Alters Human Skin Antioxidant Levels In Vivo: Case Study of Korean Adults by Resonance Raman Spectroscopy." Biotechnology and Bioprocess Engineering 23, no. 1: 116-121.

Journal article
Published: 30 January 2018 in International Journal of Molecular Sciences
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Phyllostachys nigra var. henosis, a domestic bamboo species, has been attracting much attention; its bioactive compounds (especially in the leaf) show antioxidant, anti-inflammatory, and anti-obesity activities. Little information is available on the antioxidative and anti-melanogenetic activities of the bioactive compounds in bamboo stems. The anti-melanogenic and antioxidative activities of the EtOAc fraction (PN3) of a P. nigra stem extract were investigated in a cell-free system and in B16F10 melanoma cells. PN3 consisted of a mixture of flavonoids, such as catechin, chlorogenic acid, caffeic acid, and p-coumaric acid. The antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)), and hydroxyl radical scavenging) was evaluated, as well as the inhibition of reactive oxygen species (ROS) produced by the Fenton reaction. PN3 showed in vitro tyrosinase inhibition activity with the half maximal inbihitory concentration (IC50) values of 240 μg/mL, and in vivo cytotoxic concentration ranges > 100 μg/mL. The protein expression levels and mRNA transcription levels of TYR, TRP-1, and MITF were decreased in a dose-dependent manner by the treatment with PN3. PN3 interfered with the phosphorylation of intracellular protein kinase A (PKA)/cAMP response element-binding protein (CREB), demonstrating potent anti-melanogenic effects. PN3 could inhibit PKA/CREB and the subsequent degradation of microphthalmia-associated transcription factor (MITF), resulting in the suppression of melanogenic enzymes and melanin production, probably because of the presence of flavonoid compounds. These properties make it a candidate as an additive to whitening cosmetics.

ACS Style

Moon-Hee Choi; Han-Gyo Jo; Ji Hye Yang; Sung Hwan Ki; Hyun-Jae Shin. Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells. International Journal of Molecular Sciences 2018, 19, 409 .

AMA Style

Moon-Hee Choi, Han-Gyo Jo, Ji Hye Yang, Sung Hwan Ki, Hyun-Jae Shin. Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells. International Journal of Molecular Sciences. 2018; 19 (2):409.

Chicago/Turabian Style

Moon-Hee Choi; Han-Gyo Jo; Ji Hye Yang; Sung Hwan Ki; Hyun-Jae Shin. 2018. "Antioxidative and Anti-Melanogenic Activities of Bamboo Stems (Phyllostachys nigra variety henosis) via PKA/CREB-Mediated MITF Downregulation in B16F10 Melanoma Cells." International Journal of Molecular Sciences 19, no. 2: 409.

Journal article
Published: 31 December 2017 in KSBB Journal
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ACS Style

Seung-Hwa Yang; Moon-Hee Choi; Hyun-Jae Shin. UV Protection Effect of Lignin Extracted by Steam Explosion Technique from Domestic Bamboo Stems. KSBB Journal 2017, 32, 342 -351.

AMA Style

Seung-Hwa Yang, Moon-Hee Choi, Hyun-Jae Shin. UV Protection Effect of Lignin Extracted by Steam Explosion Technique from Domestic Bamboo Stems. KSBB Journal. 2017; 32 (4):342-351.

Chicago/Turabian Style

Seung-Hwa Yang; Moon-Hee Choi; Hyun-Jae Shin. 2017. "UV Protection Effect of Lignin Extracted by Steam Explosion Technique from Domestic Bamboo Stems." KSBB Journal 32, no. 4: 342-351.

Journal article
Published: 31 December 2017 in KSBB Journal
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Bamboo stems extract containing porous silica has been used for medicine, food, paper and craft industry. Bamboo silica is known to support absorbing essential minerals such as calcium, potassium, and magnesium in skin, nail and hair. In this study, extract of domestic bamboos containing silica from two species of Phyllostachys nigra var. Enonis Stapf. and Phyllostachys bambusoides was prepared to prepare the bamboo silica materials. Physicochemical properties have been determined with XRD, FT-IR, FE-SEM and EDX. In addition, enzyme inhibition assay of collagenase and elastase has been performed. Phyllostachys nigra var. Enonis Stapf. silica (PNS) and Phyllostachys bambusoides silica (PBS) inhibited collagenase with IC50 value of 174.14 μg/mL and 292.60 μg/mL, respectively. While, positive control groups of retinol and α-tocopherol were shown to be IC50 values of 49.43 μg/ mL and 63.70 μg/mL, respectively. Furthermore, PNS and PBS elastase inhibition rate was 179.46 μg/mL and 377.03 μg/mL. These results suggest that PNS and PBS could be used as a candidate for anti-wrinkle new cosmetic ingredients.

ACS Style

Moon-Hee Choi; Da-Song Kim; Hyun-Jae Shin. Wrinkle Improvement Effect of Silica Extracted from Domestic Bamboo Stems. KSBB Journal 2017, 32, 293 -299.

AMA Style

Moon-Hee Choi, Da-Song Kim, Hyun-Jae Shin. Wrinkle Improvement Effect of Silica Extracted from Domestic Bamboo Stems. KSBB Journal. 2017; 32 (4):293-299.

Chicago/Turabian Style

Moon-Hee Choi; Da-Song Kim; Hyun-Jae Shin. 2017. "Wrinkle Improvement Effect of Silica Extracted from Domestic Bamboo Stems." KSBB Journal 32, no. 4: 293-299.

Journal article
Published: 30 September 2017 in KSBB Journal
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Fungal mycelium-based composite materials (FMBC) are a new biomaterial to replace the existing composite materials. To compete with lightweight, high-performance composite materials represented by fiber-reinforced plastic (FRP), various physical and chemical properties and functionality must be secured. Especially, the composite materials made by using mycelium of mushroom is called mushroom plastic. Currently, Ecovative, Grado Zero Espace and MycoWorks in USA and Europe are launching new products. Products utilizing FMBC can be launched in the market for construction materials, automobile interior materials and artificial leather substitutes. In spite of this high possibility, mass production using FMBC has not yet been reported. This review introduces the FMBC, a material that can replace existing plastics, inorganic building materials and animal skins in an environmentally and economically viable way, and looks at the possibility of future biomaterials by summarizing recent research contents.

ACS Style

Da-Song Kim; Yong-Woon Kim; Kil-Ja Kim; Hyun-Jae Shin. Research Trend and Product Development Potential of Fungal Mycelium-based Composite Materials. KSBB Journal 2017, 32, 174 -178.

AMA Style

Da-Song Kim, Yong-Woon Kim, Kil-Ja Kim, Hyun-Jae Shin. Research Trend and Product Development Potential of Fungal Mycelium-based Composite Materials. KSBB Journal. 2017; 32 (3):174-178.

Chicago/Turabian Style

Da-Song Kim; Yong-Woon Kim; Kil-Ja Kim; Hyun-Jae Shin. 2017. "Research Trend and Product Development Potential of Fungal Mycelium-based Composite Materials." KSBB Journal 32, no. 3: 174-178.

Biotechnology
Published: 27 September 2017 in Korean Journal of Chemical Engineering
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Gracilaria species produce agars with low quality due to their high sulfate concentrations. For this reason, the gel properties of many Gracilaria agars must be improved by appropriate processes. In this study, we developed an improved agar extraction process for Gracilaria verrucosa by alkali soaking extraction and microwave thawing and drying. Variables such as the seaweed to alkali volume ratio, extraction time, and alkali concentration were adjusted to optimize the yield and agar quality. The yield of the agar was maximized (34.5±0.6%) using 3% alkali concentration; the lowest yield (17.0±1.7%) was obtained with 1% alkali concentration. Agar gel strengths ranged from 462.0±7 to 627.3±11 g·cm−2. We have developed a process for quickly producing an agar gel through thawing and drying using microwave radiation. This study shows the possibility of producing a high-value agar using alkali soaking extraction methods for nonedible G. verrucosa.

ACS Style

Yong-Woon Kim; Hyun-Jae Shin. Introduction of alkali soaking and microwave drying processes to improve agar quality of Gracilaria verrucosa. Korean Journal of Chemical Engineering 2017, 34, 3163 -3169.

AMA Style

Yong-Woon Kim, Hyun-Jae Shin. Introduction of alkali soaking and microwave drying processes to improve agar quality of Gracilaria verrucosa. Korean Journal of Chemical Engineering. 2017; 34 (12):3163-3169.

Chicago/Turabian Style

Yong-Woon Kim; Hyun-Jae Shin. 2017. "Introduction of alkali soaking and microwave drying processes to improve agar quality of Gracilaria verrucosa." Korean Journal of Chemical Engineering 34, no. 12: 3163-3169.

Research article
Published: 27 July 2017 in Journal of Agricultural and Food Chemistry
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The pro-inflammatory response and recruitment of macrophages into adipose tissue contribute to metabolic dysfunction. Here, we reported the anti-inflammatory and antiadipogenic effects of the methanol (MeOH) extract and ethyl acetate (EtOAc) fraction of bamboo leaf and its molecular mechanism in RAW264.7 cells and 3T3-L1 adipocytes, respectively. Functional macrophage migration assays also were performed. Surprisingly, the EtOAc fraction of MeOH extracts from native Korean plant species Sasa coreana Nakai (SCN) has shown potent anti-inflammatory properties; SCN pretreatment inhibited nitric oxide (NO) production (p < 0.01) and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated macrophages. Inflammatory genes induced by LPS, including TNFα, IL-1β, and IL-6, were significantly attenuated by SCN (p < 0.01). Pretreatment with SCN antagonized NF-κB nuclear translocation and the simultaneous degradation of inhibitory κB protein. Furthermore, SCN selectively inhibited the LPS-induced phosphorylation of JNK (p < 0.01) and p38 (p < 0.05) but not ERK (p > 0.05). Similar to leaf extracts of other bamboo species, we identified that SCN contained several flavonoids including orientin, isoorientin, and vitexin; these compounds inhibited LPS-induced NO production (p < 0.05) and iNOS expression. In addition, SCN inhibited adipocyte differentiation in a dose-dependent manner, as demonstrated by Oil Red O staining and the protein expression of mature adipogenic marker genes. Treatment with the major flavonoids of SCN also inhibited adipogenesis. Furthermore, conditioned medium obtained from adipocytes stimulated macrophage chemotaxis, whereas medium from adipocytes treated with SCN significantly inhibited macrophage migration. Therefore, SCN is a potential therapeutic agent for the prevention of inflammation and obesity.

ACS Style

Ji Hye Yang; Moon-Hee Choi; Seung Hwa Yang; Sam Seok Cho; Su Jung Park; Hyun-Jae Shin; Sung Hwan Ki. Potent Anti-Inflammatory and Antiadipogenic Properties of Bamboo (Sasa coreana Nakai) Leaves Extract and Its Major Constituent Flavonoids. Journal of Agricultural and Food Chemistry 2017, 65, 6665 -6673.

AMA Style

Ji Hye Yang, Moon-Hee Choi, Seung Hwa Yang, Sam Seok Cho, Su Jung Park, Hyun-Jae Shin, Sung Hwan Ki. Potent Anti-Inflammatory and Antiadipogenic Properties of Bamboo (Sasa coreana Nakai) Leaves Extract and Its Major Constituent Flavonoids. Journal of Agricultural and Food Chemistry. 2017; 65 (31):6665-6673.

Chicago/Turabian Style

Ji Hye Yang; Moon-Hee Choi; Seung Hwa Yang; Sam Seok Cho; Su Jung Park; Hyun-Jae Shin; Sung Hwan Ki. 2017. "Potent Anti-Inflammatory and Antiadipogenic Properties of Bamboo (Sasa coreana Nakai) Leaves Extract and Its Major Constituent Flavonoids." Journal of Agricultural and Food Chemistry 65, no. 31: 6665-6673.

Review
Published: 31 March 2017 in KSBB Journal
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ACS Style

Moon-Hee Choi; Yeong-Jin Seo; Hyun-Jae Shin. Application of Domestic Bamboo Stems Mainly for Inner Beauty Product Development: A Review. KSBB Journal 2017, 32, 1 -8.

AMA Style

Moon-Hee Choi, Yeong-Jin Seo, Hyun-Jae Shin. Application of Domestic Bamboo Stems Mainly for Inner Beauty Product Development: A Review. KSBB Journal. 2017; 32 (1):1-8.

Chicago/Turabian Style

Moon-Hee Choi; Yeong-Jin Seo; Hyun-Jae Shin. 2017. "Application of Domestic Bamboo Stems Mainly for Inner Beauty Product Development: A Review." KSBB Journal 32, no. 1: 1-8.