Purpose: We tend to evaluated a method for loading 6Mercaptopurine (6MP) on green synthesized hybrid chitosan gold nanoparticles (AuNPs) forming 6MP-AuNPs nanocomposite for the first time in combinatorial chemo-photothermal therapy. Methods: The AuNPs were synthesized using chitosan as a reducing and capping agent. Different concentrations of 6MP were mixed AuNPs. Cells were incubated with 6MP and 6MP loaded AuNPs for 48 h and then exposed to laser. Results: AuNPs and 6MP-AuNPs nanocomposite have small sizes of 18 ± 4 and 25 ± 5 nm and exhibit high stability with Zeta potential of 55.9 ± 6.3 and 57 ± 4 mV. 6MP-AuNPs nanocomposite irradiated with Diode Pumped Solid State (DPSS) laser showed a maximum inhibition in cell viability reaching 63% at 1.25 µM. Conclusions: A hybrid chitosan gold nanoparticle is a powerful anti-cancer drug carrier as well as photothermal agent in combinatorial chemo-photothermal therapy.

In our present work gold nanoparticles were successfully prepared using chitosan as reducing and capping agent and subsequently used as carriers for hydrophobic anticancer 6 mercaptopurine (6MP). The use of this drug is limited due to its poor bioavailability. The purpose of the present investigation was to evaluate a method for loading hydrophobic drug 6MP on chitosan reduced gold nanoparticles (AuNPs).The resulting AuNPs and 6MP loaded AuNPs were characterized by ultraviolet visible spectrophotometry, transmission electron microscopy. To estimate the improvement of the drug delivery of AuNPs, breast cancer cells MCF7 were treated with 6MP and 6MP loaded AuNPs and cell viability was studied in vitro using Sulphorhodamine-B (SRB) assay. The data showed, both free 6MP and 6MP loaded AuNPs revealed anti-proliferation activity against the cancer cells in dose-dependent manners. Moreover, 6MP loaded AuNPs displays significantly greater anti-proliferation activity than that of free 6MP at all concentrations and IC50 1.93nM. The improvement of anti-proliferation activity of 6MP loaded AuNPs compared to that of 6MP can be attributed to enhanced intracellular uptake of the functionalized GNPs via the mechanism of endocytosis

6 mercaptopurine is a cytotoxic and immune-suppressant drug. The use of this drug is limited due to its poor bioavailability and short plasma half-life. The main objective of this study is to formulate 6 mercaptopurine (6 MP) encapsulated chitosan nanoparticles (6-MP-CNPs) using Tripolyphosphate (TPP) as cross-linker for anti-cancer therapy in order to enhance bioavailability. The prepared nanoparticles were characterized using transmission electron microscopy, zeta potential. The average particle size determined through TEM was found to be 90 ± 10 nm and after encapsulation the particle size had shown an obvious increase, Zeta potential 26.2±6.35 mV. 6 MP-CNPs showed enhancement in cellular inhibition of breast cancer cell line MCF 7 compared to free 6 MP.

6-mercaptopurine is a cytotoxic and immunosuppressant drug. The use of this drug is limited due to its poor bioavailability and short plasma half-life. Chitosan nanoparticles exhibit great interest for nanomedicine, biomedical engineering and development of new therapeutic drug release systems with improved bioavailability, increased specificity and sensitivity, and reduced pharmacological toxicity. The main objective of this study is to formulate 6mercaptopurine (6MP) encapsulated chitosan nanoparticles (6-MP-CNPs) using with Tripolyphosphate (TPP) as cross-linker for anti-cancer therapy in order to enhance cytotoxcicty and bioavailability . Chitosan nanoparticles exhibited a small particle size and a high surface charge .The prepared nanoparticles were characterized by transmission electron microscopy, zeta potential. The average particle size determined through TEM was found to be 90 ± 10 nm and after encapsulation the particle size show an obvious increase. Zeta potential 26.2±6.35 mV. 6-MP-CNPs showed enhancement in cellular inhibition of breast cancer cell line MCF7 compared to free 6MP.

To study the anticancer effect of chitosan nanoparticles (CSNPs) on a different human cancer cell lines and their possible biomedical application as anticancer, CSNPs were prepared based on the ionic gelation of chitosan with tripolyphosphate anions(TPP), The CSNPs were characterized using UV-VIS spectroscopy , transmission electron microscopy, Fourier transform infrared spectroscopy and zeta analysis. Different human cancer cell lines were used as a model. The cytotoxic effects of the CSNPs on Human breast tumour, human hepatocellular carcinoma, human lung cancer, human colon cancer and human hypopharyngeal were monitored by sulforhodamine B colorimetric assays for cytotoxicity. Treatment of different types of cancer cell lines with different concentrations of 140 nm diameter of CSNPs produce a concentration dependent decrease in cell viability and IC50 reached between 1000 and 2000 μg/ml of CSNPs after 48 h of cell incupation .These investigations implied that CSNPs could be effectively applied to improve the anticancer and nanotheraputic effect on different types of cancer.

In the present work, gold nanoparticles were successfully prepared using chitosan as reducing and capping agent and subsequently used as carriers for hydrophobic anticancer 6 mercaptopurine (6 MP). The use of this drug is limited due to its poor bioavailability. The purpose of the present investigation was to evaluate a method for loading hydrophobic drug 6 MP on chitosan reduced gold nanoparticles (AuNPs). The resulting AuNPs and 6MP loaded AuNPs were characterized using ultraviolet visible spectrophotometry and transmission electron microscopy. To estimate the improvement of the drug delivery of AuNPs, breast cancer cells MCF7 were treated with 6 MP and 6 MP loaded AuNPs and the cell viability was studied in vitro using Sulphorhodamine-B (SRB) assay. The data showed that both free 6 MP and 6 MP loaded AuNPs revealed anti-proliferation activity against the cancer cells in dose-dependent manners. Moreover, 6 MP loaded AuNPs displays significantly greater anti-proliferation activity than that of free 6 MP at all concentrations and IC50 1.93 nM. The improvement of anti-proliferation activity of 6 MP loaded AuNPs compared to that of 6 MP can be attributed to enhanced intracellular uptake of the functionalized GNPs via the mechanism of endocytosis.