This research evaluated a feed additive (benzoic acid, eugenol, thymol and piperine), associated or not with colistin, in weaned piglets feeding. The parameters evaluated were growth performance, apparent total tract digestibility (ATTD) of nutrients, diarrhea incidence, intestinal morphology, relative weights of digestive organs, microbial diversity and the percentages of operational taxonomic units of microorganisms in the caecum content of pigs. One hundred and eight crossbred piglets (5.3 ± 0.5 kg) were used in a three-phase feeding program (21-35, 36-50, 51-65 days of age), and fed a control diet with no inclusion of growth promoter feed additive, a diet with 40 ppm of colistin, a diet with 0.3% of alternative additive and a diet with 0.3% of alternative additive and 40 ppm of colistin. The diets were based on corn, soybean meal, dairy products, and spray-dried blood plasma, formulated to provide 3.40, 3.38, and 3.20 Mcal of ME/kg; and 14.5, 13.3, and 10.9 g/kg of digestible lysine, in phases 1, 2, and 3, respectively. The piglets were housed three per pen, with 9 replicates per diet, in a complete randomized block design based on initial BW. The data was submitted to ANOVA and means were separated by Tukey test (5%), using SAS. Pigs fed diets with the alternative feed additive had greater (P < 0.05) ADG (114.3 g vs. 91.8 g) and ADFI (190.1 g vs. 163.3) in phase 1 than pigs fed diets without the product. The alternative additive improved (P < 0.05) ATTD of CP in phase 1 (71.0% vs. 68.6%), GE in phases 1 (77.4% vs. 75.2%) and 3 (79.0% vs. 77.1%), and DM in phase 3 (79.1% vs. 77.1%). The antibiotic inclusion in the diets increased (P < 0.05) ATTD of CP in phase 1 (71.5% vs. 68.2%). The alternative feed additive tended (P = 0.06) to increase (46%) normal feces frequency, decreased (P < 0.05) goblet cells count (104.3 vs. 118.1) in the jejunum, and decreased (P < 0.05) small intestine (4.60% vs. 4.93%) and colon (1.41% vs. 1.65%) relative weights, compared to pigs not fed with the alternative additive. There was a tendency (P = 0.09) for a lower concentration of Escherichia-Shigella (1.46 vs. 3.5%) and lower (P < 0.05) percentage of Campylobacter (0.52 vs. 10.21%) in the cecum content of piglets fed diets containing essential oils and benzoic acid compared to pigs fed diets without the alternative feed additive. The alternative feed additive was effective in improving growth performance, diets digestibility and gut health in piglets soon after weaning.

Two experiments were performed to determine the digestibility of diets with crude protein (CP) reduction supplemented with amino acids (18.15; 17.15; 16.15 and 15.15%) to growing pigs (30–50 kg), to assess the use of nutrients and account for the manure excretion, and to evaluate the performance, backfat thickness, Longissimus lumborum depth, and plasma urea, aiming to evaluate the environmental impact through life cycle assessment (LCA); for the first time in Brazil interacting experiments were developed to evaluate the CP reduction and LCA. The CP reduction resulted in greater daily weight gain (p = 0.011), final weight (p = 0.020), better use of N and P, through the greater N and P retained (p = 0.003 and p = 0.017, respectively). There was a linear reduction in acidification potential (p = 0.015), eutrophication potential (p = 0.001), and land occupation (p = 0.005) when dietary CP decreased from 18.15 to 15.15%. The reduction in CP and supplementation of amino acids in diets for growing pigs (30–50 kg) improved final and daily weight gain. Through LCA, and performance and metabolism data, it was concluded that for the acidification, eutrophication and land occupation categories, impacts were reduced as the protein concentration was reduced.

SUMMARY The objective of this study was to determine the chemical composition, apparent metabolizable energy (AME), apparent metabolizable energy corrected for nitrogen balance (AMEn) and the metabolizability of dry matter (DM), crude protein (CP), ether extract (EE) and neutral detergent fiber (NDF) of passion fruit seed meal (PFM) for quails. One hundred meat quails (Coturnix coturnix japonica), with 21 days old, allotted in a completely randomized design, with four treatments, five replicates and four animals per experimental unit. The treatments consisted of different levels PFM (4.0, 8.0, 12.0 and 16.0%) that replaced the basal diet (w/w). Metabolizable values were determined by fitting linear equations the PFM can be characterized as an alternative feedstuff for meat quails and the main chemical compounds are gross energy (5,569 kcal kg-1), CP (11.34%), EE (18.84%) and NDF (50.22%), as fed basis. Linear equations were fitted as follow: AME = 2,976 x + 5.877 (r2 = 0.94) and AMEn = 2,939 x + 4.864 (r2 = 0.95), thus AME and AMEn were estimated at 2,976 and 2,939 kcal kg-1, respectively. Additionaly, equations were adjusted for CP, EE, NFD and DM, respectively resulting in a metabolization of 6.35, 17.9, 12.48 and 45.66%. It is concluded that main components of the passion fruit seed meal are gross energy, crude protein, ether extract and neutral detergent fiber and its AME and AMEn for meat quails are 2,976 and 2,939 kcal kg-1, respectively.

Three experiments (exp.) were conducted to determine and compare the digestibility of nutrients and energy of corn distillers dried grains with solubles (DDGS) from the United States (USDDGS), a dried mixture of corn bran with solubles (CBS) from Brazil (BRCBS), and high protein corn distillers dried grains (HP-DDG) from the United States (USHPDG) and Brazil (BRHPDG) in growing pigs. The feed ingredients were evaluated for apparent total tract digestibility (ATTD) of gross energy (GE), dry matter (DM), crude protein (CP), ether extract, neutral and acid detergent fiber (NDF and ADF, respectively), and digestible and metabolizable energy (DE and ME, respectively) using the total collection and index methods in exp. 1; ATTD and standardized total tract digestibility (STTD) of phosphorus (P) in exp. 2; and apparent (AID) and standardized (SID) ileal digestibilities of CP and amino acids (AA) in exp. 3. Fifty crossbred barrows (32.4 ± 6.9, 38.3 ± 5.2, and 46.2 ± 5.3 kg body weight [BW], in exp. 1, 2, and 3, respectively) were fed a corn basal diet in exp. 1, a P-free diet in exp. 2, and an N-free diet in exp. 3 or diets with 40% inclusion of test ingredients to provide 10 replications per treatment. Pigs were housed individually in metabolism cages (exp. 1) or in pens (exp. 2 and 3) and fed at 2.8 times the maintenance DE requirement (110 kcal/kg BW^0.75) based on their BW at the beginning of each experiment. Except for ATTD of NDF, which tended (P = 0.058) to be greater by the index method compared with the total collection method, no difference between the total collection and index methods was observed for ATTD of remaining nutrients and DE. The ATTD of DM, GE, NDF, and DE content of BRHPDG were greater (P < 0.001) than USHPDG, BRCBS, and USDDGS. The AID of CP, Arg, His, Ile, Leu, Lys, Thr, and Val and the SID of His, Leu, Lys, and Val of BRHPDG were 8% to 36% greater (P < 0.05) than those from USHPDG. Except for Trp, all AID and SID AA values were greater (P < 0.05) in BRHPDG than in USHPDG. The ATTD of DM, GE, NDF, and ADF; DE and ME content; AID of CP, Arg, Ile, Leu, Phe, Thr, and Trp; and SID of CP, Arg, Phe, and Thr of USDDGS were 9% to 45% greater (P < 0.05) than those in BRCBS. The ATTD and the STTD of P in USHPDG and USDDGS were 26% to 42% greater (P < 0.05) compared with BRHPDG and BRCBS. In conclusion, BRHPDG had a greater digestibility of energy and most of the AA than USHPDG, while the BRCBS evaluated had lower nutritional value than the USDDGS source.

The chemical composition of corn is variable and the knowledge of its chemical and energetic composition is required for an accurate formulation of the diet. This study aimed to determine the chemical composition, that is, dry matter (DM), mineral matter (MM), neutral detergent fiber (NDF), acid detergent fiber (ADF), ether extract (EE), crude protein (CP), gross energy (GE) and energetic values of different varieties (batches) of corn and validate mathematical models to predict the metabolizable energy values (ME) of corn for pigs using near infrared spectroscopy (NIRS). Corn samples were scanned in the spectrum range between 1,100 and 2,500 nm, the model parameters were estimated by the modified partial least squares (MPLS) method. Ten prediction equations were inserted into the NIRS and used to estimate the ME values. The first degree linear regression models of the estimated ME values in function of the observed ME values were adjusted. The existence of a linear ratio was evaluated by detecting the significance to posterior estimates of the straight line parameters. The values of digestible energy and ME ranged from 3,400 to 3,752 and 3,244 to 3,611 kcal kg⁻¹, respectively. The prediction equations, ME₁ = 4334 – 8.1MM + 4.1EE – 3.7NDF; ME₂ = 4,194 – 9.2MM + 1.0CP + 4.1EE – 3.5NDF; and ME₇ = 16.13 – 9.5NDF + 16EE + (23CP × NDF) – (138MM × NDF) were the most adequate to predict the ME values of corn by using NIRS.