Leandro Dalcin Castilha

Dr. Leandro Dalcin Castilha

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Research Focus & Metrics

Academic Career Length

15Years (2012-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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25 April 2021 in Sustainability
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LELucas Esteves
Lucas Esteves
AMAlessandra Monteiro
Alessandra Monteiro
NSNatália Y. Sitanaka
Natália Y. Sitanaka
POPaula C. Oliveira
Paula C. Oliveira
LCLeandro Dalcin Castilha
Leandro Dalcin Castilha
PPPaulo Cesar Pozza
Paulo Cesar Pozza
Two experiments were performed to determine the digestibility of diets with crude protein (CP) reduction supplemented with amino acids (18.15; 17.15; 16.15 and 15.15%) to growing pigs (30–50 kg), to assess the use of nutrients and account for the manure excretion, and to evaluate the performance, backfat thickness, Longissimus lumborum depth, and plasma urea, aiming to evaluate the environmental impact through life cycle assessment (LCA); for the first time in Brazil interacting experiments were developed to evaluate the CP reduction and LCA. The CP reduction resulted in greater daily weight gain (p = 0.011), final weight (p = 0.020), better use of N and P, through the greater N and P retained (p = 0.003 and p = 0.017, respectively). There was a linear reduction in acidification potential (p = 0.015), eutrophication potential (p = 0.001), and land occupation (p = 0.005) when dietary CP decreased from 18.15 to 15.15%. The reduction in CP and supplementation of amino acids in diets for growing pigs (30–50 kg) improved final and daily weight gain. Through LCA, and performance and metabolism data, it was concluded that for the acidification, eutrophication and land occupation categories, impacts were reduced as the protein concentration was reduced.

31 March 2023 in Acta Scientiarum. Animal Sciences
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JNJailson Novodworski
Jailson Novodworski
LCLeandro Dalcin Castilha
Leandro Dalcin Castilha
ASAlessandra Aparecida Silva
Alessandra Aparecida Silva
The present study aims to conduct a review on the potential use of insect meal as an alternative protein source in poultry feed, particularly to serve the rearing of free-range chickens. Insects are already part of the diet of birds in their natural habitat, and the availability of low-cost alternative foods with low environmental impact is essential for the development of the activity. The review comprehended studies that used meals consisting of silkworm (Bombyx mori) chrysalis, earthworm (Eisenia foetida), housefly (Musca domestica), black soldier fly (Hermetia illucens) and mealworm beetle (Tenebrio molitor) to replace plant-based protein sources. In general, insect meals have a high content of crude protein and ether extract, as well as an essential amino acid profile suitable for poultry feeding. The addition of insect meal in poultry feed normally shows good results as to growth performance and egg production, without causing a negative effect on carcass characteristics, meat sensory quality and egg quality, presenting itself as an alternative protein source with good prospects for replacing plant-based sources in poultry feed.

28 August 2024 in Animals
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DDDaniel Dutra
Daniel Dutra
Erick Alonso Villegas-CayllahuaErick Alonso Villegas-Cayllahua
Erick Alonso Villegas-Cayllahua
GBGiovanna Garcia Baptista
Giovanna Garcia Baptista
LFLucas Emannuel Ferreira
Lucas Emannuel Ferreira
LCLeandro Dalcin Castilha
Leandro Dalcin Castilha
HBHirasilva Borba
Hirasilva Borba
The aim of the present study was to evaluate the characteristics of carcasses, monitor their pH evolution during the first 24 h post-mortem, and determine the time required for the establishment and resolution of rigor mortis in different categories of Botucatu rabbits. Live weight at slaughter, carcass weight, and yield were higher in 12-month-old animals compared to 3-month-old rabbits, regardless of sex. There was an effect of muscle type, age, and sex on the kinetics of muscle acidification, with the Biceps femoris showing a significantly higher pH than the Longissimus lumborum from 4 h post-mortem onward. The establishment of rigor mortis occurred at 5 h post-mortem in young rabbits and bucks and at 6 h post-mortem in does, along with pH stabilization, while the resolution of rigor occurred at 18 h post-mortem for all types of carcasses evaluated. In conclusion, Botucatu rabbit carcasses should be chilled continuously at 4 °C for a minimum of 18 h to ensure efficient rigor mortis progression and muscle-to-meat transformation throughout the carcass.

24 November 2022 in Pharmaceutics
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KCKatieli Silva Souza Campanholi
Katieli Silva Souza Campanholi
RJRanulfo Combuca da Silva Junior
Ranulfo Combuca da Silva Junior
RGRenato Sonchini Gonçalves
Renato Sonchini Gonçalves
MOMariana Carla de Oliveira
Mariana Carla de Oliveira
MPMagali Soares Dos Santos Pozza
Magali Soares Dos Santos Pozza
WCWilker Caetano
Wilker Caetano
Chronic wound healing represents an impactful financial burden on healthcare systems. In this context, the use of natural products as an alternative therapy reduces costs and maintains effectiveness. Phytotherapeutic gels applied in photodynamic therapy (PDT) have been developed to act as topical healing medicines and antibiotics. The bioactive system is composed of Spirulina sp. (source of chlorophylls) and Copaifera reticulata oil microdroplets, both incorporated into a polymeric blend constituted by kappa-carrageenan (k-car) and F127 copolymer, constituting a system in which all components are bioactive agents. The flow behavior and viscoelasticity of the formulations were investigated. The photodynamic activity was accessed from studies of the inactivation of Staphylococcus aureus bacteria, the main pathogen of hospital relevance. Furthermore, in vivo studies were conducted using eighteen rabbits with dermatitis (grade III and IV) in both paws. The gels showed significant antibiotic potential in vitro, eliminating up to 100% of S. aureus colonies in the presence or absence of light. The k-car reduced 41% of the viable cells; however, its benefits were enhanced by adding chlorophyll and copaiba oil. The animals treated with the phytotherapeutic medicine showed a reduction in lesion size, with healing and re-epithelialization verified in the histological analyses. The animals submitted to PDT displayed noticeable improvement, indicating this therapy’s viability for ulcerative and infected wounds. This behavior was not observed in the iodine control treatment, which worsened the animals’ condition. Therefore, gel formulations were a viable alternative for future pharmaceutical applications, aiming at topical healing.

14 October 2022 in The Scientific World Journal
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GOGislaine Gonçalves Oliveira
Gislaine Gonçalves Oliveira
EGEliane Gasparino
Eliane Gasparino
LCLeandro Dalcin Castilha
Leandro Dalcin Castilha
NMNilton Garcia Marengoni
Nilton Garcia Marengoni
Elenice Souza Dos Reis GoesElenice Souza Dos Reis Goes
Elenice Souza Dos Reis Goes
MSMaria Luiza Rodrigues de Souza
Maria Luiza Rodrigues de Souza
This study aimed to compare the resistance of the Oryctolagus cuniculus L. (rabbit) and Oreochromis niloticus L. (Nile tilapia) skins, as well as to observe the design of the flower of these skins and the morphology of the dermis. Tilapia and rabbit skins were placed inside the same equipment (tannery machine) for the chromium salt tanning process. The flower design of the fish leather distinguishes it from the rabbit leather, the latter being constituted by the opening of the hair follicles and pores, while the fish leather is constituted by the presence of protective lamellae and insertion of the scales. The dermis of rabbit skin consists of thick bundles of collagen fibers arranged in all directions, which differs from the morphology observed in the dermis of fish skin. However, in the Nile tilapia skin dermis, overlapping and parallel layers of longitudinal collagen fiber bundles are observed, these layers are interspersed with fiber bundles crossing the sking surface (transversely), tying the fibers together and providing greater strength, which can be proven by the strength test. The fish leathers, despite having less thickness (1.0mm), demonstrated significantly greater tensile strength (13.521.86Nmm−2) and tear strength (53.856.66Nmm−2) than rabbit leathers, that is, (8.982.67Nmm−2) and (24.254.34Nmm−2). However, rabbit leather demonstrated higher elasticity (109.9713.52) compared to Nile tilapia leather (78.978.40). It can be concluded that although the rabbit leather is thicker due to the histological architecture of the dermis (thick bundles of collagen fibers arranged in all directions with no pattern of organization of collagen fibers), it shows less resistance than Nile tilapia leather, which demonstrates an organization of overlapping and parallel layers and intercalating collagen fiber bundles transversally to the surface, functioning as tendons for the swimming process. It is recommended to use a piece of fabric (lining) together with the fleshy side of the rabbit leather, to increase resistance when used in clothing and footwear, as these products require greater tensile strength. Thus, it minimizes this restriction for the use of rabbit leather in the aforementioned purposes.

19 August 2024 in Acta Scientiarum. Animal Sciences
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SLSilvio Mayke Leite
Silvio Mayke Leite
ESEriem Micaela Gonçalo Sena Santos
Eriem Micaela Gonçalo Sena Santos
MAMariana Regolin Almeida
Mariana Regolin Almeida
NONatalia Oliva
Natalia Oliva
GSGabriella Goulart Stevanato
Gabriella Goulart Stevanato
LCLeandro Dalcin Castilha
Leandro Dalcin Castilha
This review aimed to address all relevant parts of the digestive physiology of rabbits, before and after weaning, with a view to enabling greater understanding of these phases and reducing the mortality of kits. The biggest bottlenecks in Brazilian rabbit farming are linked to the period from birth to weaning, a time when the animals are more sensitive to environmental adverse events, requiring more rigid and efficient management due to their immune system being not fully developed. Throughout the period in which kits are with the females, many physiological changes occur, mainly in their gastrointestinal tract (GIT), which changes over time, depending on the type of food intake; in order to achieve its full capacity to utilize food, the intestine needs to undergo an adaptation from milk-based to solid diet. Thus, the digestive system in the intrauterine phase, in the nursing kits, and in the weaned babies will be covered. Therefore, understanding the physiology of baby rabbits proves to be of great value in reducing the mortality rate, so that management becomes more practical, providing producers with different problem-solving alternatives, in addition to greater profit.