• Facile ultrasound-assisted Turkevich method for gold nanoparticles synthesis. • Increase in power of ultrasound affects the reduction time on the chemical reaction and allow homogeneity control. • In the disproportionation process, a complex tetrachloroaurate is recycled for the two-electron reduction by citrate.

Currently, superparamagnetic functionalized systems of magnetite (Fe₃O₄) nanoparticles (NPs) are promising options for applications in hyperthermia therapy, drug delivery and diagnosis. Fe₃O₄ NPs below 20 nm have stable single domains (SSD), which can be oriented by magnetic field application. Dispersion of Fe₃O₄ NPs in silicon dioxide (SiO₂) matrix allows local SSD response with uniaxial anisotropy and orientation to easy axis, 90° or 180° . A successful, easy methodology to produce Fe₃O₄ NPs (6–17 nm) has been used with the Stöber modification. NPs were embedded in amorphous and biocompatible SiO₂ matrix by mechanical stirring in citrate and tetraethyl orthosilicate (TEOS). Fe₃O₄ NPs dispersion was sampled in the range of 2–12 h to observe the SiO₂ matrix formation as time function. TEM characterization identified optimal conditions at 4 h stirring for separation of SSD Fe₃O₄ in SiO₂ matrix. Low magnetization (M_s) of 0.001 emu and a coercivity (H_c) of 24.75 Oe indicate that the embedded SSD Fe₃O₄ in amorphous SiO₂ reduces the M_s by a diamagnetic barrier. Magnetic force microscopy (MFM) showed SSD Fe₃O₄ of 1.2 nm on average embedded in SiO₂ matrix with uniaxial anisotropy response according to Fe³⁺ and Fe²⁺ electron spin coupling and rotation by intrinsic Neél contribution.

Nanomaterials with enzyme-like activity have been the spotlight of scientific and technological efforts to substitute natural enzymes, not only in biological research but also for industrial manufacturing, medicine, and environment healing. Notable advancements in this field along the last years relied on to the rational design of single-atom active sites, knowledge of the underlying atomic structure, and realistic ab initio theoretical models of the electronic configuration at the active site. Thus, it is plausible that a next generation of nanozymes still to come will show even improved catalytic efficiency and substrate specificity. However, the dynamic nature of the protein cage surrounding most active sites in biological enzymes adds a flexible functionality that possess a challenge for nanozyme's mimicking of their natural counterparts. We offer a perspective about where the main strategies to improve nanozymes are headed and identify some of the big challenges faced along the road to better performance. We also outline some of the most exciting bio-inspired ideas that could potentially change this field.