Mixed land use provides an important means of promoting the intensive and efficient use of land resources and stimulating endogenous development power in rural areas. This paper selected Pingba Village in Chongqing as the research area; the land use status data and the social and economic data on rural settlements in the study area for 2021 were obtained through field visits and interviews. Moreover, the land use types in the rural settlements were subdivided according to the principle of dominant function. Based on these subdivisions, a land mixed-use measurement system for rural settlements was constructed to analyze their levels of mixed land use. Furthermore, the influences of natural environmental, social, economic and other factors on mixed land use were comprehensively explored. The results showed that, (1) the mixed land use of rural settlements in the study area was at a medium level and showed significant spatial variability, and rural settlements in the high, medium and low mixed land use index zones accounted for 12.5%, 35% and 52.5% of the total, respectively. (2) The differences in the natural environment determined the level of mixed land use and the basic pattern of its spatial differentiation. Social and economic factors, such as resident population and average household income, were key impact factors. Rural tourism resources, homestead agglomeration policies and other factors had important impacts on the level of mixed land use. In conclusion, the research suggests that mixed land use is an important way to boost rural revitalization. In the future, village planning could introduce the concept of mixed land use to improve the efficiency of land use, optimize the land use structure according to local conditions and promote the integrated development of rural primary, secondary and tertiary industries. In addition, it is necessary to scientifically and rationally guide rural settlements to agglomerate appropriately to improve the utilization efficiency of land resources and public service resources.