In recent years, the changes experienced in the educational and social field have been reflected in the growing interest in defining how the attributes of the learning space affect students. This study seeks to determine the influence exerted by the design and organization of the university classroom on motivation and social interaction. The objective was to develop and validate a measurement instrument to evaluate the impact of the university classroom design on the student, considering their physical, environmental, perceptual, and motivational attributes. The questionnaire was applied to a sample of 402 students from the University of Almeria (Spain). The reliability and content validity of the construct were analyzed. Reliability was demonstrated by a Cronbach’s alpha of 0.938 for the dimensions with quantitative cut items, being highly satisfactory. The Chi-square goodness of fit test analyzed the qualitative items. Content validity was subject to expert judgment. It is concluded that the presented instrument meets the required technical attributes, to be considered a reliable and valid educational tool for measurement.