Shiga-toxin-producing Escherichia coli (STEC) is typically detected on food products mainly due to cross-contamination with faecal matter. The serotype O157:H7 has been of major public health concern due to the severity of illness caused, prevalence, and management. In the food chain, the main methods of controlling contamination by foodborne pathogens often involve the application of antimicrobial agents, which are now becoming less efficient. There is a growing need for the development of new approaches to combat these pathogens, especially those that harbour antimicrobial resistant and virulent determinants. Strategies to also limit their presence on food contact surfaces and food matrices are needed to prevent their transmission. Recent studies have revealed that bacteriophages are useful non-antibiotic options for biocontrol of E. coli O157:H7 in both animals and humans. Phage biocontrol can significantly reduce E. coli O157:H7, thereby improving food safety. However, before being certified as potential biocontrol agents, the safety of the phage candidates must be resolved to satisfy regulatory standards, particularly regarding phage resistance, antigenic properties, and toxigenic properties. In this review, we provide a general description of the main virulence elements of E. coli O157:H7 and present detailed reports that support the proposals that phages infecting E. coli O157:H7 are potential biocontrol agents. This paper also outlines the mechanism of E. coli O157:H7 resistance to phages and the safety concerns associated with the use of phages as a biocontrol.

Antibiotics are regularly used in animal husbandry to treat diseases. This practice is beneficial to animals’ health and helps ensure food security. However, the misuse of antibiotics, especially in food-producing animals, has resulted in the advent of antimicrobial resistance (AMR) and its dissemination among foodborne pathogens. The occurrence of AMR in bacteria pathogens that cause infections in animals and those associated with food spoilage is now considered a global health concern affecting humans, animals and the environment. The search for alternative antimicrobial agents has kindled the interest of many researchers. Among the alternatives, using plant-derived nanoparticles (PDNPs) for treating microbial dysfunctions in food-producing animals has gained significant attention. In traditional medicine, plant extracts are considered as safe, efficient and natural antibacterial agents for various animal diseases. Given the complexity of the AMR and concerns about issues at the interface of human health, animal health and the environment, it is important to emphasize the role of a One Health approach in addressing this problem. This review examines the potential of PDNPs as bio-control agents in food-producing animals, intending to provide consumers with microbiologically safe food while ensuring food safety and security, better health for animals and humans and a safe environment.

Diarrhea is a condition in which feces is discharged from the bowels frequently and in a liquid form. It is one of the frequent causes of morbidity and mortality in developing countries. The impact of Diarrhea is worsened by the increasing incidence of antimicrobial resistance among the causative agents, and this is now categorized as a global healthcare challenge. Antimicrobial resistance among Diarrheal pathogens also contributes to extended infection durations, and huge economic loss even in countries with advanced public health policies. The ever-increasing incidence of antimicrobial resistance including the contraindications arising from the administration of antibiotics in some Diarrheal cases highlights a crucial need for the development of novel non-antibiotic alternative agents for therapeutic and biocontrol applications. One such intervention includes the application of plant-derived nanoparticles (PDNPs) with novel antimicrobial properties. Given their small size and large surface area to volume ratio, PDNPs can attack target bacterial cell walls to generate reactive oxygen species that may simultaneously disrupt bacteria cell components such as DNA and proteins leading to cell damage or death. This potential can make it very difficult for pathogenic organisms to develop resistance against these antibacterial agents. In this review, we provide a critical overview on the antimicrobial resistance crisis among Diarrheagenic bacteria. We also discuss the evidence from the existing literature to support the potential associated with the use of PDNPs as alternative therapeutic agents for multidrug resistant and antibiotics administer contraindicated bacteria that are associated with Diarrhea.

Antimicrobial‐resistant Escherichia coli , especially those belonging to the serotype O157, are increasingly linked to foodborne diseases with significant fatality rates worldwide. The food and medical industries have focused on E. coli O157:H7 due to its ability to produce toxins coupled with its low infectious dose. The aim of this study was to assess the virulome, resistome, and pathogenicity of E. coli O157:H7 using whole genome sequencing. Three previously isolated E. coli O157:H7 strains from cattle feces were subjected to whole genome sequencing. The genome sizes of all three E. coli O157:H7 strains were 5,117,276 bp, 5,039,443 bp, and 5,034,351 bp. The C + G contents were 50.22%, 50.53%, and 50.54%, while the number of contigs was 110, 43, and 42, respectively, for E. coli O157:H7 strains J32, J57, and J69. Several virulence determinants (hemorrhagic E. coli pilus (HCP), eaeA, hemolysin, etc.) were found in the genomes of these isolates. In addition, antibiotic resistance genes conferring resistance to aminoglycosides, tetracyclines, macrolides, fluoroquinolones, penams, carbapenems, cephalosporins, cephamycin, rifamycin, phenicols, monobactams, and nitroimidazole were found in the genomes. Interestingly, the genomes of these isolates also harbored determinants encoding resistance to disinfectants and antiseptics, indicating their concern in the food production and medical sectors. This highlights the public health concerns of these isolates, indicating the need for constant surveillance.

The high mortality and morbidity resulting from diarrhoeal cases worldwide are associated with the increasing incidence of antimicrobial resistance (AMR) and represent a serious public health concern. Cattle are a major reservoir of AMR organisms, and faecal shedding may facilitate their transmission into the food chain. This study examined the pathotype, biofilm, virulome and resistome profiles of bacteria isolated from cattle faeces using whole genome sequencing (WGS). Asymptomatic cattle faecal samples ( n = 269) were analysed, and three isolates identified as multidrug‐resistant and biofilm‐forming bacteria were sequenced. In this study, we successfully isolated bacteria from cattle faecal samples, and the isolates DEC_NWU, DVC_NWU and DSS_NWU were phenotypically confirmed as multidrug‐resistant and strong biofilm formers. WGS analysis confirmed DEC_NWU, DVC_NWU and DSS_NWU to have genome lengths of 4,803,571, 4,499,945 and 5,374,783 bp, respectively. The Genome Taxonomy Database (GTDB) confirmed that DEC_NWU, DVC_NWU and DSS_NWU are E. coli, V. cholerae and S. enterica, respectively. Also, the genetic nexus of the isolates with other species confirmed that DEC_NWU, DVC_NWU and DSS_NWU were related to E. coli O104:H4 (88%), V. cholerae O1 (100%) and S. enterica serovar Typhimurium (100%), respectively. In addition, PathogenFinder classified the isolates as human pathogens. Furthermore, virulence factors such as adherence, iron uptake, invasion, toxin and secretion system were detected in the genomes of DEC_NWU, DVC_NWU and DSS_NWU. The DSS_NWU genome had the highest number of virulence genes (65), compared to DVC_NWU (29) and DEC_NWU (40). Notably, DEC_NWU, DVC_NWU and DSS_NWU each harboured several resistance genes. This study established that asymptomatic cattle carry human pathogens, which harbour a repertoire of virulome and resistome.