As a potential adsorbent material, loose, porous livestock manure biochar provides a new approach to livestock manure resource utilization. In this study, coconut husks (CH) and livestock manure, i.e., cow dung (CD), pig manure (PM), and chicken manure (CM) were used as biomass precursors for preparation of biochar via high-temperature pyrolysis and CO₂ activation. Characterization technologies, such as scanning electron microscopy, Fourier transform infrared spectroscopy, adsorption–desorption isotherms, and pore size distributions, were used to study the microscopic morphologies and physicochemical properties of unactivated and activated biochar materials. The results showed that CD biochar provides better adsorption performance (up to 29.81 mg H₂S/g) than CM or PM biochar. After activation at 650° for 1 h, the best adsorption performance was 38.23 mg H₂S/g. For comparison, the CH biochar removal performance was 30.44 mg H₂S/g. Its best performance was 38.73 mg H₂S/g after 1 h of activation at 750 °C. Its best removal performance is equivalent to that of CH biochar activated at a temperature that is 100 °C higher. Further material characterization indicates that the H₂S removal performance of livestock-manure–derived biochar is not entirely dependent on the specific surface area, but is closely related to the pore size distribution.

Fluorescence excitation–emission matrix (EEM) spectroscopy is a powerful tool for characterizing dissolved organic matter (DOM), a key component of anaerobic digestion. In this study, the fluorescence characteristics of DOM during 55 days of anaerobic digestion of oil crop straw inoculated with rumen liquid were investigated. EEM spectroscopy coupled with parallel factor analysis (PARAFAC) showed that three major fluorescence components, tyrosine- (C₁), humic- (C₂) and tryptophan-like substances (C₃), were identified in all DOM samples. The F_max values of C₁ and C₃ increased rapidly during the first 5 d, decreased dramatically from day 5 to day 35, and then remained stable, while C₂ was not biodegraded. The changes in the F_max values of the fluorescence components reflected the biodegradation of lignin and/or embedded cellulose by rumen microorganisms. The changes in the Stokes shift of the fluorescence peak were readily explained by the variation in the hydrophobic/hydrophilic fraction distribution. The humidification index (HIX) and A : T ratio of the DOM decreased after 5 d and then increased gradually. Compared with the McKnight fluorescence index (MFI), the Y fluorescence index (YFI) was better able to track the evolution of the DOM. Correlation analysis of the different fluorescence indices (intensities) and absorbance indices was also carried out. The EEM-PARAFAC individual components, HIX and A : T ratio were conveniently used to characterize the degree of anaerobic conversion of the organic matter, and the peak at the Stokes shift of ∼1.0 μm⁻¹ was used as one of the indicators showing the stabilization of anaerobic digestion. These findings may assist in developing fluorescence technology for monitoring the anaerobic digestion of crop straw.