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I am working as Assistant Professor from the last nine years in Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan. My field of research is Pharmaceutical Microbiology and nanomedicine and my PhD research work was “Study on Methicillin-Resistant Staphylococcus aureus and Vancomycin-Resistant Enterococci Co-Colonization in Patients of Intensive Care Units of Tertiary Health Care Facilities (Hospitals)”.
Nitric oxide maintains the integrity of the gastric epithelium and is also elevated in diarrheal disease condition. Nitric oxide synthase isoforms (NOS) are responsible for production of nitric oxide (NO) in physiological and pathophysiological circumstances. Zinc supplementation has been used as part of treatment of infants and children with diarrheal illnesses in most developing nations. The aim of this study was to establish the effect of zinc-fortification on fecal nitric oxide and colonal inducible nitric oxide synthase gene in enterotoxigenic E. coli (ETEC) induced zinc-deficient diarrhea. Albino rats were grouped into normal fed (NF), zinc deficient (ZD) and zinc fortified (ZF) groups with control, LT-ETEC induced and ST-ETEC induced diarrheal sub-groups. Nitric oxide concentrations were determined in feces using griess reaction system while reverse transcription polymerase chain reaction with GAPDH gene as internal reference was used to monitor the presence of colonal inducible NOS gene. The amount of stool output was significantly (p˂0.05) increased in normal fed and zinc deficient LT [661±2.00], [760±2.22] and ST-induced [772±2.00], [886±2.12] groups when compared with the control [555±3.03], [600±3.11] groups respectively. Fecal nitric oxide significantly increased in LT [82.09±1.30], [132.24±2.24] and ST-ETEC [68.81±2.30], [88.21±1.14] induced groups in NF and ZD rats when compared with controls [54.27±1.23], [66.86±2.01] respectively. When compared with both NF and ZD rats, fecal NO decreased significantly in ZF rats in both LT [48.06±0.96] and ST-induced [45.48±0.60] groups. The colonal inducible nitric oxide synthase gene was not detected in NF, ZD and ZF rats. Zinc deficiency increased both stool output and fecal nitric oxide in ETEC induced diarrhea, while zinc-fortification reduced stool output and restores fecal nitric oxide. This suggests that zinc and nitric oxide play a role in mediating pathological conditions in ETEC-induced diarrhea of which inducible nitric oxide synthase may not be involved.
Ebuka E. David; Muhammad A. Yameen; Ikechuku O. Igwenyi; Chidinma N. David. Zinc-fortification restores gut nitric oxide without expression of inducible nitric oxide synthase gene in enterotoxigenic E. coli-induced diarrhea in zinc-deficient rats. Scientific African 2021, e00867 .
AMA StyleEbuka E. David, Muhammad A. Yameen, Ikechuku O. Igwenyi, Chidinma N. David. Zinc-fortification restores gut nitric oxide without expression of inducible nitric oxide synthase gene in enterotoxigenic E. coli-induced diarrhea in zinc-deficient rats. Scientific African. 2021; ():e00867.
Chicago/Turabian StyleEbuka E. David; Muhammad A. Yameen; Ikechuku O. Igwenyi; Chidinma N. David. 2021. "Zinc-fortification restores gut nitric oxide without expression of inducible nitric oxide synthase gene in enterotoxigenic E. coli-induced diarrhea in zinc-deficient rats." Scientific African , no. : e00867.
The Paeonia emodi (P. emodi)-mediated iron oxide nanoparticles (Fe2O3 NPs) were screened for in-vitro and in-vivo antibacterial activity against the Staphylococcus aureus (S. aureus) (ATCC #: 6538) and Escherichia coli (E. coli) (ATCC #:15224). The synthesized Fe2O3 NPs were characterized via nitrogen adsorption-desorption process, X-ray diffractometer (XRD), transmission and scanning electron microscopies (TEM and SEM), energy dispersive X-ray (EDX) and Fourier transform infrared (FTIR) spectroscopies. The SBET was found to be 94.65 m2/g with pore size of 2.99 nm, whereas the average crystallite and particles size are 23 and 27.64 nm, respectively. The 4 μg/mL is the MIC that inhibits the growth of E. coli, whereas those for S. aureus are below the detection limit (<1.76 μg/mL). The tolerance limit of the mice model was inspected by injecting different concentration of Fe2O3 NPs and bacteria suspensions. The 14 ppm suspension was the tolerated dose and the concentration above were proved lethal. The most severe infection was induced in mice with injection of 3 × 107 CFUs of both bacteria, while the inoculation of higher concentrations of bacterial suspensions resulted in the mice’s death. The histopathological and hematological studies reveals that the no/negligible infection was found in the mice exposed to the simultaneous inoculation of Fe2O3 NPs (14 ppm) and bacterial suspensions (3 × 107 CFUs).
Amreen Shah; Isfahan Tauseef; Manel Ali; Muhammad Yameen; Amine Mezni; Amor Hedfi; Syed Haleem; Sirajul Haq. In-Vitro and In-Vivo Tolerance and Therapeutic Investigations of Phyto-Fabricated Iron Oxide Nanoparticles against Selected Pathogens. Toxics 2021, 9, 105 .
AMA StyleAmreen Shah, Isfahan Tauseef, Manel Ali, Muhammad Yameen, Amine Mezni, Amor Hedfi, Syed Haleem, Sirajul Haq. In-Vitro and In-Vivo Tolerance and Therapeutic Investigations of Phyto-Fabricated Iron Oxide Nanoparticles against Selected Pathogens. Toxics. 2021; 9 (5):105.
Chicago/Turabian StyleAmreen Shah; Isfahan Tauseef; Manel Ali; Muhammad Yameen; Amine Mezni; Amor Hedfi; Syed Haleem; Sirajul Haq. 2021. "In-Vitro and In-Vivo Tolerance and Therapeutic Investigations of Phyto-Fabricated Iron Oxide Nanoparticles against Selected Pathogens." Toxics 9, no. 5: 105.