Loggerhead sea turtle (C. caretta) is the official European bioindicator of marine litter in the Mediterranean Sea. In 2019, 8 sea turtles, out of 28 specimens loggerhead on the Adriatic coast of Molise, were subjected to necropsy. The intestinal contents were collected and the microplastics until 0.45 μm were extracted. Qualitative and quantitative assessments were performed by stereomicroscope observation and spectroscopic analyses (attenuated total reflection-Fourier transform infrared spectroscopy, ATR-FTIR and Raman microspectroscopy, RMS). Moreover, the analytical quantification of polyethylene terephthalate (PET), polycarbonate (PC), para phthalic acid (PTA) and bisphenol A (BPA) in fat and liver tissues was performed by LC-MS/MS. Microparticles ranging from 0.45 μm to 1 mm were found in all turtles, for a total of 623, while plastic litter greater than 1 mm were found only in 4 specimens (ranging from 0.03 to 0.11 g). Nineteen different polymers and 10 pigments, including polyester (100% of animals), high-density polyethylene (50%) and polypropylene (50%) were identified. BPA, PTA and PET were detected in fat and liver tissues of all animals, while PC was found only in 50%. A major prevalence was registered in the abdominal fat tissue, although only PC compounds were significantly higher in abdominal tissue (p < 0.05), except for free PTA with liver tissue being the most contaminated (p < 0.05). Microplastics and additives surely impact the health status of turtles that showed gastrointestinal impairment and an important level of contamination in tissues. Graphical abstract

The determination of trace metals in organic matrices is still highly demanding despite improvements in analytical instrumentation. The present study was undertaken in order to evaluate electrothermal atomic absorption spectrometry for the determination of cadmium, copper, iron, lead, and nickel in olive oil. A variety of approaches were used. The most suitable digestion procedure was heating the samples at 300°C for 24 hours and ashing in a muffle furnace at 450°C for 16 hours. The validation data were detection limits of 0.2–153 ng g−1; mean trueness on certified reference materials of 81–94%; mean recovery on spikes of 90–120%; and repeatability of 12–53%. The combined relative uncertainty was 0.298–0.766. Oils processed by pressing had higher copper, iron, and lead concentrations than oils processed by centrifugation. The reported method provides an efficient way for monitoring trace metal content during olive oil production.

Due to the simplicity of tea preparation (pouring hot water onto different dried herbs) and its high popularity as a beverage, monitoring and developing a screening methodology for detecting the metal content is very important. The concentrations of Cd, Ca, Cu, Fe, Pb, Mg and Mn in 11 different samples of sage (Salvia officinalis L.), linden (Tilia L.) and chamomile (Matricaria chamomilla L.) purchased at local herbal pharmacy were determined using electrothermal atomizer atomic absorption spectrometry (ETAAS) and flame atomizer atomic absorption spectrometry (FAAS). The concentrations determined were: Cd (0.012 – 0.470 mg kg⁻¹), Ca (5209 – 16340 mg kg⁻¹), Cu (22.01 – 33.05 mg kg⁻¹), Fe (114.2 – 440.3 mg kg⁻¹), Pb (0.545 – 2.538 mg kg⁻¹), Mg (2649 – 4325 mg kg⁻¹) and Mn (34.00 – 189.6 mg kg⁻¹). Principal Component Analysis (PCA) was applied to identify factors (soil and climate) influencing the content of the measured elements in herbal samples. The proposed methodology developed in this work was successfully applied to the detection of metals in herbal samples. The analysis showed that the content of toxic metals in herbal teas was below the maximum dose recommended by the World Health Organization (WHO).

We describe a new method using flow-injection analysis with spectro-photometric detection, suitable for the determination of N-acetyl-L-cysteine (NAC). The proposed method is appropriate for the determination of NAC in reaction with Pd²⁺ ions in the concentration range from 1.0 × 10⁻⁵ mol L⁻¹ to 6.0 × 10⁻⁵ mol L⁻¹. The detection limit NAC was 5.84 × 10⁻⁶ mol L⁻¹ and the recorded relative standard deviation of the method is in the range from 1.67 to 4.11%. NAC and Pd²⁺ form complexes of Pd²⁺:NAC molar ratios of 1:1 and 1:2, depending on the ratio of their analytical concentrations. The cumulative conditional stability constant for the Pd(NAC)₂²⁺ complex is β₁₂' = 2.69 × 10⁹ L² mol⁻². The proposed method was compared with the classic spectrophotometric determination of NAC, using the same reagent, PdCl₂, and had shown certain advantages: a) shorter analysis time; b) the use of smaller volumes of sample and reagents, which make the proposed method cheaper and faster for NAC determination in real samples without sample pretreatment.

Due to the simplicity of tea preparation (pouring hot water onto different dried herbs) and its high popularity as a beverage, monitoring and developing a screening methodology for detecting the metal content is very important. The concentrations of Cd, Ca, Cr, Cu, Fe, Pb, Mg, Mn, Hg, Na and Zn in 8 samples of green tea (Camellia sinesis) and in 11 samples chamomile (Matricaria chamomilla L.) purchased both at local herbal pharmacies and supermarkets were determined using electrothermal atomizer atomic absorption spectrometry (ETAAS) and flame atomizer atomic absorption spectrometry (FAAS). The found concentrations in chamomile were: Cd (0.008 – 284 mg kg⁻¹), Ca (2.42 – 6.29%), Cr (0.91 – 6.92 mg kg⁻¹), Cu (6.27 – 11.39 mg kg⁻¹), Fe (133.5 – 534 mg kg⁻¹), Pb (0.561 – 1.277 mg kg⁻¹), Mg (2.27 – 3.73%), Mn (62.2 – 165.6 mg kg⁻¹), Hg (0.660 – 1.346 μg kg⁻¹), Na (0.91 – 1.28%) and Zn (63.37 – 108.5 mg kg⁻¹), in green tea Cd (36.29 – 202.1 mg kg⁻¹), Ca (2.77 – 6.40%), Cr (1.520 – 5.278 mg kg⁻1), Cu (9.354 – 22.56 mg kg⁻¹), Fe (162.6 – 513.3 mg kg⁻¹), Pb (1.808 – 4.770 mg kg⁻¹), Mg (1.41 – 2.62 %), Mn (1.147 – 1.729 g kg⁻¹), Hg (1.045 – 2.802 μg kg⁻¹), Na (0.44 – 0.98%) and Zn (30.65 – 115.6 mg kg⁻¹), respectively. Principal Component Analysis (PCA) was applied to identify factors (soil, climate and country of origin) influencing the content of the measured elements in herbal samples. The proposed methodology developed in this work was successfully applied to the detection of metals in herbal samples. The analysis showed that the content of toxic metals in green tea samples was significantly higher and very close to the maximum dose recommended by the World Health Organization (WHO).