Subcutaneous fat and marbling both increase in beef cattle during the feeding phase but are antagonistic in regard to their contribution to beef carcass value. The objective of this study was to determine whether cellular factors associated with marbling development change with growth stage throughout the feeding period and whether they are correlated to marbling relative to carcass composition. Twenty-four steers of known origin with the cytosine and thymine (CT) leptin genotype were allotted to 3 harvest groups. Six steers per harvest group were harvested at the following predetermined points: 35 d on feed (early feeding period, EF), average live weight of 464 kg (middle feeding period, MF), and 1.17-cm 12th-rib subcutaneous fat thickness (late feeding period, LF). Longissmus muscle samples were collected within 30 min postmortem and snap frozen for real-time PCR and Western blot analysis of lipoprotein lipase, adenosine monophosphate–activated protein kinase α (AMPKα), stearoyl-coenzyme A desaturase (SCD), PPARγ, C/EBP-β, and myostatin. Carcass data were recorded, and LM samples were collected and aged 2, 7, 14, and 21 d postmortem for Warner-Bratzler shear force determination. Carcass composition was estimated by dissection of the 9–10–11 rib section and subsequent proximate analysis of the soft tissue. Intramuscular fat content of the LM increased linearly throughout the feeding period, giving additional support to marbling as an early developing tissue. Expression of AMPKα was found to be downregulated, whereas SCD expression was upregulated in the LF group relative to the first 2 harvest groups. Additionally, SCD and PPARγ were downregulated in the EF group relative to the latter 2 harvest groups. These changes in gene expression resulted in a linear increase in only PPARγ protein abundance, whereas myostatin tended to increase quadratically. A correlation was found between intramuscular fat and PPARγ abundance. This gives further evidence of the importance of adipocyte hyperplasia in increasing marbling. Targeting and increasing PPARγ expression may serve as a mechanism to increase marbling deposition. Last, LF steaks were more tender than MF or EF steaks, indicating improved tenderness with increased days on feed. Copyright © 2014. American Society of Animal Science .

The objective of this study was to determine the influence of two finishing systems (grain- or grass-finishing) on carcass characteristics, meat quality, nutritional composition, and sensory attributes of bison. Bison heifers were assigned to either a grain- or grass-finishing treatment for 130 days prior to slaughter. Carcass measurements, lean color and fat color were recorded. Striploins (M. longissimus lumborum) were collected for analysis of pH, fatty acid profile, cholesterol, proximate analysis, Warner-Bratzler shear force, cook loss, and consumer sensory evaluation. Grain-finished bison heifers had greater (p < 0.01) hot carcass weights, dressing percentage, ribeye area, backfat, and marbling scores compared to grass-finished heifers. Instrumental color values (L*, a*, b*) of the ribeye and a* value of backfat opposite the ribeye were greater (p < 0.01) for grain-finished heifers. Steaks from grain-finished heifers had increased (p < 0.05) crude protein and fat content and decreased (p < 0.01) moisture compared to grass-finished heifers. The grain-finishing system produced steaks with increased (p < 0.01) cholesterol and total fatty acids (mg/g of wet tissue). The grain-finished system produced more tender (p < 0.05) steaks, but consumer sensory ratings did not differ (p > 0.10) between treatments. These data indicate that finishing systems influence bison carcass characteristics, nutritional composition, and meat quality, but do not translate to differences in consumer preferences.

Electrical stimulation (ES) is used in beef slaughter plants to improve tenderness; however, varying levels of low-voltage ES have not been well characterized. The objective was to evaluate the influence of two levels of low-voltage ES on temperature decline, pH, glycolytic potential, and meat quality. Forty-two beef carcasses were chosen from a commercial packing facility. One side of each carcass received either 40 or 80 volts of ES for 60 s at 45 min postmortem. The paired side of each carcass did not receive ES (Control). Temperature loggers were placed in the sirloin of 12 carcasses to record temperature decline. Longissimus muscle pH was measured at 1, 12, and 24 h, and 3 d postmortem. Strip steaks were fabricated for determination of meat quality. A treatment by time interaction was observed for carcass temperature decline (p < 0.001) where ES sides stayed warmer longer than Control sides. A treatment by time interaction was observed for pH decline with Control sides exhibiting an increased pH at 1 h postmortem (p < 0.001). Instrumental color values were increased for ES compared to Control sides (p < 0.001). These results indicate ES slows carcass temperature decline, hastens initial pH decline, and improves instrumental color. Similar results were observed between the ES treatments indicating either ES level may be used to achieve similar quality characteristics.

The objective of this study was to determine the influence of grain- and grass-finishing systems on carcass characteristics of bison bulls and proximate and fatty acid compositions of bison steaks. Bison bulls grazed native pasture until approximately 25 mo of age, then were randomly assigned to grain-finishing (n=98) or grass-finishing (n=98) treatments. Bulls were slaughtered at approximately 30 mo of age. Hot carcass weight (HCW), ribeye area, backfat thickness, kidney fat percentage, marbling score, and instrumental color (L*,a*, and b*) of the ribeye and subcutaneous fat were recorded. Skeletal maturity, lean maturity, and fat color were subjectively scored. Strip loins were collected from a sub-sample of carcasses, fabricated into 2.5-cm steaks, and designated for proximate, cholesterol, or fatty acid analyses. Grain-finished bulls had greater (P<0.0001) HCW, dressing percentage, ribeye area, backfat thickness, kidney fat percentage,and marbling score. The a* and b* values of the ribeye and a* value of subcutaneous fat were greater (P<0.0001), but the L* and b* values of subcutaneous fat were less (P<0.0001) for grain-finished bulls. A greater proportion (P<0.001) of grain-finished carcasses had moderately bright red lean color, whereas a greater proportion (P<0.0001) of grass-finished carcasses had moderately yellow fat color. Steaks from grain-finished bulls had an increased percentage of crude protein (P<0.0001), fat (P<0.0001), and ash (P=0.0006) content but less moisture (P<0.0001). Steaks from grain-finished bulls had more (P<0.001) cholesterol and palmitic, stearic, oleic, linoleic, and arachidonic acids in addition to more total fatty acids (mg/g of wet tissue). However, for total fatty acids, grass-finished steaks had a greater (P<0.0001) percentage of polyunsaturated fatty acids. These data indicate that the finishing system influences the composition of bison bull carcasses as well as the nutrient profile of bison meat.

The concept of fetal programming is based on the idea that nutritional status and environmental conditions encountered by the dam during pregnancy can have lifetime impacts on her offspring. These changes in the gestational environment have been shown to influence fetal development and subsequent growth performance, carcass composition, and meat quality characteristics. Beef fetuses can be particularly prone to experiencing variations in the maternal environment during development owing to a relatively long duration of pregnancy potentially exposing the dam to environmental temperature stress and/or seasonal conditions that can compromise feed quality or quantity. If feed is limited or forage conditions are poor, a maternal deficiency in protein and/or energy can occur as well as fluctuations in body condition of the dam. As a result, the fetus may receive inadequate levels of nutrients, potentially altering fetal development. There are critical windows of development during each stage of gestation in which various tissues, organs, and metabolic systems may be impacted. Skeletal muscle and adipose tissue are particularly vulnerable to alterations in the gestational environment because of their low priority for nutrients relative to vital organs and systems during development. The timing and severity of the environmental event or stressor as well as the ability of the dam to buffer negative effects to the fetus will dictate the developmental response. Much of the current research is focused on the influence of specific nutrients and timing of nutritional treatments on offspring carcass composition and meat quality, with the goal of informing strategies that will ultimately allow for the use of maternal nutritional management as a tool to optimize performance and meat quality of offspring.