Myocardial remodeling after myocardial infarction (MI) is associated with increased levels of the matrix metalloproteinases (MMPs). Levels of two MMP species, MMP-2 and MMP-9, are increased after MI, and transgenic deletion of these MMPs attenuates post-MI left ventricular (LV) remodeling. This study characterized the spatiotemporal patterns of gene promoter induction for MMP-2 and MMP-9 after MI. MI was induced in transgenic mice in which the MMP-2 or MMP-9 promoter sequence was fused to the β-galactosidase reporter, and reporter level was assayed up to 28 days after MI. Myocardial localization with respect to cellular sources of MMP-2 and MMP-9 promoter induction was examined. After MI, LV diameter increased by 70% ( P < 0.05), consistent with LV remodeling. β-Galactosidase staining in MMP-2 reporter mice was increased by 1 day after MI and increased further to 64 ± 6% of LV epicardial area by 7 days after MI ( P < 0.05). MMP-2 promoter activation occurred in fibroblasts and myofibroblasts in the MI region. In MMP-9 reporter mice, promoter induction was detected after 3 days and peaked at 7 days after MI (53 ± 6%, P < 0.05) and was colocalized with inflammatory cells at the peri-infarct region. Although MMP-2 promoter activation was similarly distributed in the MI and border regions, activation of the MMP-9 promoter was highest at the border between the MI and remote regions. These unique findings visually demonstrated that activation of the MMP-2 and MMP-9 gene promoters occurs in a distinct spatial relation with reference to the MI region and changes in a characteristic time-dependent manner after MI.

The formation of a self-consistent crystalline structure is a well-known phenomenon in complex plasmas. In most experiments, the pressure and rf power are the main controlling parameters in determining the phase of the system. We have studied the effect of the externally applied magnetic field on the configuration of plasma crystals, suspended in the sheath of a radio-frequency discharge using the Magnetized Dusty Plasma Experiment device. Experiments are performed at a fixed pressure and rf power where a crystalline structure is formed within a confining ring. The magnetic field is then increased from 0 to 1.28 T. We report on the breakdown of the crystalline structure with the increasing magnetic field. The magnetic field affects the dynamics of the plasma particles and first leads to a rotation of the crystal. At a higher magnetic field, there is a radial variation (shear) in the angular velocity of the moving particles which we believe to lead to the melting of the crystal. This melting is confirmed by evaluating the variation of the pair correlation function as a function of magnetic field.

We revisit the inflationary dynamics in detail for theories with Gauss-Bonnet gravity coupled to scalar functions, in light of the Planck data. Considering the chaotic inflationary scenario, we constrain the parameters of two models involving inflaton-Gauss-Bonnet coupling by current Planck data. For nonzero inflaton-Gauss-Bonnet coupling $\beta$, an inflationary analysis provides us a big cosmologically viable region in the space of ($m$, $\beta$), where $m$ is the mass of the inflaton. However, we study further on constraining $\beta$ arising from reheating considerations and unitarity of tree-level amplitude involving 2-graviton $\to 2$-graviton ($hh\to hh$) scattering. Our analysis, particularly on reheating significantly reduces the parameter space of ($m$, $\beta$) for all models. The quadratic Gauss-Bonnet coupling parameter turns out to be more strongly constrained than that of the linear coupling. For the linear Gauss-Bonnet coupling function, we obtain $\beta \lesssim 10^3$, with the condition $\beta (m/M_P{)}^2\simeq {10}^{-4}$. However, the study of the Higgs inflation scenario in the presence of a Gauss-Bonnet term turns out to be completely disfavored.