The objective of this study was to estimate genetic parameters for age at first calving (AFC) and first calving interval (FCI) for the entire beef cattle population and separately for the Charolais (CH) and Aberdeen Angus (AA) breeds in the Czech Republic. The database of performance testing between the years 1991 and 2019 was used. The total number of cows was 83,788 from 11 breeds. After editing, the data set contained 33,533 cows, including 9321 and 4419 CH and AA cows, respectively. The relationship matrix included 85,842 animals for the entire beef population and 24,248 and 11,406 animals for the CH and AA breeds, respectively. A multibreed multitrait animal model was applied. The estimated heritability was low to moderate. Genetic correlations between AFC and FCI varied depending on the breeds from positive to negative. Differences between variance components suggest that differences between breeds should be considered before selection and breeding strategy should be developed within a breed.

Mitochondrial DNA and nonrecombinant parts of Y-chromosome DNA are a great tool for looking at a species’ past. They are inherited for generations almost unaffected because they do not participate in recombination; thus, the time of occurrence of each mutation can be estimated based on the average mutation rate. Thanks to this, male and female haplogroups guide confirming events in the distant past (potential centers of domestication, settlement of areas, trade connections) as well as in modern breeding (crossbreeding, confirmation of paternity). This research focuses mainly on the development of domestic sheep and its post-domestication expansion, which has occurred through human trade from one continent to another. So far, five mitochondrial and five Y-chromosome haplogroups and dozens of their haplotypes have been detected in domestic sheep through studies worldwide. Mitochondrial DNA variability is more or less correlated with distance from the domestication center, but variability on the recombinant region of the Y chromosome is not. According to available data, central China shows the highest variability of male haplogroups and haplotypes.

High-quality simple sequence repeat (SSR) markers are invaluable tools for revealing genetic variability which could be utilized for many purposes, such as breeding new varieties or the identifying current ones, among other applications. Based on the analysis of 3.7 million EST sequences and 15 genomic sequences from bacterial artificial chromosome (BAC) libraries, 200 trinucleotide genic (EST)-SSR and three genomic (gSSR) markers were tested, where 17 of them fulfilled all criteria for quality markers. Moreover, the reproducibility of these new markers was verified by two genetics laboratories, with a mean error rate per allele and per locus equal to 0.17%. These markers were tested on 38 accessions of Papaver somniferum and nine accessions of another five species of the Papaver and Argemone genera. In total, 118 alleles were detected for all accessions (median = 7; three to ten alleles per locus) and 88 alleles (median = 5; three to nine alleles per locus) within P. somniferum alone. Multivariate methods and identity analysis revealed high resolution capabilities of the new markers, where all but three pair accessions (41 out of 47) had a unique profile and opium poppy was distinguished from other species.

The aim of this study was a genome-wide association study (GWAS) on conformation traits using 25,486 genotyped Czech Holsteins, with 35,227 common SNPs for each genotype. Linear trait records were collected between 1995 and 2020. The Interbull information from Multiple Across Country Evaluation (MACE) was included for bulls that mostly had daughter records in a foreign country. When using the Bonferroni correction, the number of SNPs that were either significant or approached the significance threshold was low—dairy capacity composite on BTA4, feet and legs composite BTA21, total score BTA10, stature BTA24, body depth BTA6, angularity BTA20, fore udder attachment BTA10. Without the Bonferroni correction, the total number of significant or near of significance SNPs was 32. The SNPs were localized on BTA1,2,4,5,6,7,8,18,22,25,26,28 for dairy capacity composite, BTA15,21 for feet and legs composite, BTA10 for total score, BTA24 stature, BTA6,23 body depth, BTA20 angularity, BTA2 rump angle, BTA9,10 rear legs rear view, BTA2,19 rear legs side view, BTA10 fore udder attachment, BTA2 udder depth, BTA10 rear udder height, BTA12 central alignment, BTA24 rear teat placement, BTA8,29 rear udder width. The results provide biological information for the improvement of body conformation and fitness in the Holstein population.

The structure of the breed in 2003 was: 39 sires and 350 dams. The breed was closed against immigration in 1992. In 1993 and 2003 coefficients of inbreeding (F_x) were calculated from 5 parental generations for sires and dams in the whole breed and white and black variety. From 1993 to 2003 decreased the F_x (%) in the breed for stallions from 5.65 to 5.57 and for mares from 7.75 to 4.88. The effective population size was in 1993 N_e=114.00 and 2003 N_e=140.36. The increment of N_e during the 10-year period was 26.36 animals. The average heterozygosity estimated on the frequencies of blood types, biochemical markers and microsatellites was enough large and was connected with a low coefficient of inbreeding. Linear type evolutions of 494 horses of the Old Kladrub breed for 32 traits were used to analyse the effect of variety and stud. The data were used for the estimation of population parameters and heritability coefficients. The specific properties and variation of the Old Kladrub horse in its current state were characterized by the overall mean, standard deviation, coefficient of variation, number of utilized scores and heritabilities. The highest coefficient of variation showed the forelimbs-side view (40.14 %), chest girth (36.25 %) and height at withers (30.97 %). The 8 traits showed heritabilities within the interval of 0.20 to 0.68. These traits are candidates as selection criteria. Significant differences between both varieties were found in 13 from 32 traits. Significant differences were found in 12 of 32 traits between the Kladruby stud and the private studs.