The extraction of bitumen from the Albertan oilsands produces large amounts of oil sands process-affected water (OSPW) that requires remediation. Classical naphthenic acids (NAs), a complex mixture of organic compounds containing O₂⁻ species, are present in the acid extractable organic fraction of OSPW and are a primary cause of acute toxicity. A potential remediation strategy is combining chemical oxidation and biodegradation. Persulfate as an oxidant is advantageous, as it is powerful, economical, and less harmful towards microorganisms. This is the first study to examine persulfate oxidation coupled to biodegradation for NA remediation. Merichem NAs were reacted with 100, 250, 500, and 1000 mg/L of unactivated persulfate at 21 °C and 500 and 1000 mg/L of activated persulfate at 30 °C, then inoculated with Pseudomonas fluorescens LP6a after 2 months. At 21 °C, the coupled treatment removed 52.8–98.9% of Merichem NAs, while 30 °C saw increased removals of 99.4–99.7%. Coupling persulfate oxidation with biodegradation improved removal of Merichem NAs and chemical oxidation demand by up to 1.8× and 6.7×, respectively, and microbial viability was enhanced up to 4.6×. Acute toxicity towards Vibrio fischeri was negatively impacted by synergistic interactions between the persulfate and Merichem NAs; however, it was ultimately reduced by 74.5–100%. This study supports that persulfate oxidation coupled to biodegradation is an effective and feasible treatment to remove NAs and reduce toxicity.

Oil sands process‐affected water (OSPW) is a by‐product of bitumen extraction from oil sands surface mining in Alberta, Canada. A major group of organics in OSPW known as naphthenic acid fraction compounds (NAFCs) are of concern due to their persistence and toxicity. Constructed wetland treatment systems have emerged as potential biological treatment approaches for reducing NAFC concentrations within OSPW. In this study, greenhouse‐scale mesocosms simulating a constructed wetland consisting of coarse sand tailings (CST) and OSPW were used to evaluate the ability of Scirpus microcarpus, Triglochin maritima, and unplanted controls to attenuate NAFCs under spring/fall and summer temperatures (10°C/5°C and 20°C/10°C day/night). Overall, in this mesocosm system, NAFC attenuation was similar regardless of different design parameters such as plant type, plant presence, and temperature. By the end of the study, NAFCs attenuation was 30% to 50% lower than the initial OSPW depending on plant species, plant presence, and temperature. The relative abundance of the acutely toxic O₂‐NAFCs decreased over time, with an increase in the less toxic O₃, O₄, and SO₃ classes. Various hydrocarbon‐degrading microbial families such as Comamonadaceae and Xanthobacteraceae were found to be dominant in OSPW, while cyanobacteria (Trichormus) were enriched in the CST. Principal component analysis indicated that only time led to distinct clusters for NAFC composition, while plant type, temperature, and time influenced the microbial communities. Shifts in microbial communities over time corresponded to shifts in NAFCs, possibly due to a decrease in toxicity with increased oxidation of NAFCs and/or an increase in available nutrients from a decrease in plant fitness in the planted mesocosms.Practitioner Points: Constructed wetland mesocosms for NAFC attenuation from OSPW comparing three planted/unplanted conditions under two temperatures. Mesocosms had 30%–50% removal of total NAFCs, with a decrease in O₂‐NAFCs and increase in O₃, O₄, and SO₃ classes. NAFC composition only shifted with time, while microbial communities were influenced by plant type, temperature, and time. Lack of difference in NAFC attenuation between treatments could indicate a high level of functional redundancy between the microbial communities.

Surface mining and bitumen extraction in Alberta's oil sands generates various tailings waste streams as by-products. Among these tailings, froth treatment tailings (FTT), originating from the froth treatment process, are particularly complex due to high levels of iron sulfide minerals (i.e. pyrite) with acid mine drainage (AMD) potential. Paraffinic FTT that are passed through a Tailings Solvent Recovery Unit (TSRU) to recover residual solvent are referred to as TSRU tailings. This study evaluated the ability of TSRU tailings to support upland and wetland vegetation under various thin capping designs using peat mineral soil mix (PMM) and coarse sand tailings (CST). A 30 cm PMM cover cap successfully supported upland and wetland vegetation for three years, while a thicker, multi-layer cap of 30 cm PMM above 20 cm of CST further improved plant survival and growth. The cap was found to effectively act as a barrier, protecting the vegetation from the adverse effects of pyrite oxidation in the TSRU tailings. While the TSRU tailings acidified (pH ≤ 2) under all capping treatments, the thickest cap (30 cm PMM/20 cm CST) delayed acidification by one year. TSRU and CST had low microbial biomass and diversity, with bacterial communities mostly composed of sulfur oxidizers and acidophilic taxa. In contrast, the PMM layer maintained a higher microbial biomass, diversity, and stability across all treatments, highlighting its potential to enhance plant-microbe interactions and improve the reclamation success of TSRU tailings.