An isocratic reversed-phase high-performance liquid chromatography (RP-HPLC) method has been developed for rapid and simultaneous separation and estimation of 3 antidiabetic drugs, namely, metformin, pioglitazone, and glimepiride, in human plasma within 3 min. Separation was carried out on a MAGELLEN 5U C₁₈ (5 μm, 150 mm × 4.60 mm) using a mobile phase of MeOH–0.025 M KH₂PO₄ adjusted to pH 3.20 using ortho-phosphoric acid (85:15, v/v) at ambient temperature. The flow rate was 1 mL/min, and the maximum absorption was measured at 235 nm. The retention time of metformin, pioglitazone, and glimepiride was noted to be 1.24, 2.32, and 2.77 min, respectively, indicating a very short analysis time compared to that of other reported methods. Also, limits of detection were reported to be 0.05, 0.26, and 0.10 μg/mL for metformin, pioglitazone, and glimepiride, respectively, showing a high degree of method sensitivity. The method was then validated according to the FDA guidelines for the determination of the three drugs clinically in human plasma, in particular, regarding pharmacokinetic and bioequivalence simulation studies.

Quality and safety of drugs are essential for effective therapeutic performance. Impurities can compromise the quality and safety of drugs, and they can arise during various stages of the development, production, storage and even transportation process. Therefore, detecting and measuring the number of impurities with high accuracy in drugs is necessary to ensure the quality and safety of drugs and to reduce the risks associated with taking them. Detecting and measuring impurities in drugs require advanced analytical techniques. The review highpoints a variety of analytical chemistry techniques include spectrophotometric and chromatographic methods in addition to some electrochemistry methods that have been applied for determination of certain drugs such as Ciprofloxacin, Metronidazole, Hydroxychloroquine and Cefotaxime in their pure form, combined form with other drugs, combined form with degradation products, and in biological fluids.

Two spectrophotometric procedures are presented for the determination of three commonly used corticosteroid drugs. The procedures are based on the formation of the phenylhydrazones of the corticosteroids, which were subsequenly subjected to a charge-transfer complexation reaction with either the σ-acceptor iodine or the π-acceptor chloranil. The molar combining ratio and the optimum assay conditions were studied. Application of these procedures to the assay of the corticosteroids in tablet form is described. The mean recoveries were 99.26 ± 0.86, 99.67 ± 0.64 and 99.72 ± 1.39% using iodine as the acceptor reagent and 99.5 ± 0.78, 99.64 ± 0.62 and 99.9 ± 0.78% using chloranil as the acceptor reagent for prednisone, prednisolone and dexamethasone, respectively. The results obtained compared favourably with those obtained by the BP method.