Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) is a worldwide pest that damages over 900 host plant species. The volatile organic compounds (volatiles) of contrasting plants, as well as their growth stage, influence this pest’s infestation behavior. The chemical contents of volatiles isolated from three plants (Gossypium hirsutum, Abutilon theophrasti, and Ricinus communis) during various growth phases (pre-flowering, fluorescence, and fruiting) were examined, as well as their influence on the behavior of adult B. tabaci. The olfactometer studies demonstrated that growth periods of the three plants affected the preference of B. tabaci. Volatiles of piemarker and cotton plants had dissimilar levels of attraction to adults during all stages. Volatile substances released by the castor at the stage of flowering had repellent effect on B. tabaci. In the plant versus plant combination, piemarker volatiles before and during anthesis were most preferred by adults, followed by cotton and then castor. A total of 24, 24, and 20 compounds were detected from volatiles of piemarker, cotton, and castor, respectively, and proportions among the compounds changed during different stages of plant development. The olfactory responses of B. tabaci to volatile compounds presented that linalool and high concentration of (Z)-3-hexenyl acetate had a strong trapping effect on this pest, while nonanal had a significant repellent effect at high concentration. This study indicates that distinct plants and their growth stage affect their attractiveness or repellency to B.tabaci adults, which are mediated by changing plant volatiles. These compounds obtained by analysis screening can be adopted as potential attractants or repellents to control Mediterranean (MED) B. tabaci.

With the wide-scale adoption of transgenic Bacillus thuringiensis (Bt) cotton, Apolygus lucorum (Meyer-Dür) has become the most serious pest and has caused extensive yield loss in cotton production. However, little is known about the defense responses of cotton at the seedling stage to A. lucorum feeding. In this study, to elucidate the cotton defense mechanism, cotton leaves were damaged by A. lucorum for 0, 4, 12 and 24 h. The transcriptomic results showed that A. lucorum feeding elicits a rapid and strong defense response in gene expression during the whole infestation process in cotton plants. Further analysis revealed that at each assessment time, more differentially expressed genes were up-regulated than down-regulated. The integrated analysis of transcriptomic and metabolic data showed that most of the genes involved in jasmonic acid (JA) biosynthesis were initially up-regulated, and this trend continued during an infestation. Meanwhile, the content levels of JA and its intermediate products were also significantly increased throughout the whole infestation process. The similar trend was displayed in condensed tannins biosynthesis. This research proved that, after plants are damaged by A. lucorum, the JA pathway mediates the defense mechanisms in cotton plants by promoting the accumulation of condensed tannins as a defense mechanism against A. lucorum. These results will help us to discover unknown defensive genes and improve the integrated pest management of A. lucorum.

Plants would release herbivore-induced plant volatiles (HIPVs) to repel herbivores and attract natural enemies after being damaged by herbivores. In this study, after cotton plants were damaged by different densities of Apolygus lucorum, the behavioral responses of A. lucorum and Peristenus spretus to cotton plants volatiles were evaluated, and the quality and quantity of volatiles from cotton plants were analyzed. Only when cotton plants were damaged by four bugs did both A. lucorum and P. spretus show an obvious response to damaged cotton plants, which indicates that cotton defense is correlated with pest density. The collection and analysis of volatiles reveals that the increase in pest density results in the emission of new compounds and an increase in the total number of volatiles with an alteration in proportions among the compounds in the blend. These changes in volatile profiles might provide wasps and mirids with specific information on host habitat quality and thus could explain the behavioral responses of parasitoids and pests.

The bacterial communities of arthropods play important roles in the growth and development of their hosts, such as by providing nutrition or protecting against parasites and pathogens. However, for Tetranychus truncatus, a major crop pest, very little is known about its bacterial community. To understand the effects of the bacterial community on T. truncatus, mites were fed bean leaves that had been soaked in different concentrations of tetracycline hydrochloride (5 mg to 5 g per L). Antibiotic treatment had a negative impact on the fitness of T. truncatus, decreasing their fecundity and longevity. However, we could not exclude the possibility that the decrease in fecundity was due to a toxic effect of the antibiotic. 16S rRNA analyses showed that the antibiotic treatment did not significantly affect the diversity of bacterial species, but it did significantly change their relative abundances. Furthermore, predicted functions of microbiota were also changed with antibiotic treatment. Our research revealed that the antibiotic induced change of bacterial communities mostly decreased the fecundity of T. truncatus, although we couldn’t totally exclude the direct toxin of antibiotics on spider mites.