Understanding how different areas of the human brain communicate with each other is a crucial issue in neuroscience. The concepts of structural, functional and effective connectivity have been widely exploited to describe the human connectome, consisting of brain networks, their structural connections and functional interactions. Despite high-spatial-resolution imaging techniques such as functional magnetic resonance imaging (fMRI) being widely used to map this complex network of multiple interactions, electroencephalographic (EEG) recordings claim high temporal resolution and are thus perfectly suitable to describe either spatially distributed and temporally dynamic patterns of neural activation and connectivity. In this work, we provide a technical account and a categorization of the most-used data-driven approaches to assess brain-functional connectivity, intended as the study of the statistical dependencies between the recorded EEG signals. Different pairwise and multivariate, as well as directed and non-directed connectivity metrics are discussed with a pros–cons approach, in the time, frequency, and information-theoretic domains. The establishment of conceptual and mathematical relationships between metrics from these three frameworks, and the discussion of novel methodological approaches, will allow the reader to go deep into the problem of inferring functional connectivity in complex networks. Furthermore, emerging trends for the description of extended forms of connectivity (e.g., high-order interactions) are also discussed, along with graph-theory tools exploring the topological properties of the network of connections provided by the proposed metrics. Applications to EEG data are reviewed. In addition, the importance of source localization, and the impacts of signal acquisition and pre-processing techniques (e.g., filtering, source localization, and artifact rejection) on the connectivity estimates are recognized and discussed. By going through this review, the reader could delve deeply into the entire process of EEG pre-processing and analysis for the study of brain functional connectivity and learning, thereby exploiting novel methodologies and approaches to the problem of inferring connectivity within complex networks.

The brain–computer interfaces (BCI) are interfaces that put the user in communication with an electronic device based on signals originating from the brain. In this paper, we describe a proof of concept that took place within the context of BciAi4Sla, a multidisciplinary project involving computer scientists, physiologists, biomedical engineers, neurologists, and psychologists with the aim of designing and developing a BCI system following a user-centered approach, involving domain experts and users since initial prototyping steps in a design–test–redesign development cycle. The project intends to develop a software platform able to restore a communication channel in patients who have compromised their communication possibilities due to illness or accidents. The most common case is the patients with amyotrophic lateral sclerosis (ALS). In this paper, we describe the background and the main development steps of the project, also reporting some initial and promising user evaluation results, including real-time performance classification and a proof-of-concept prototype.

So far, very little attention has been paid to the role of the autonomic nervous system in augmentative alternative communication solutions. In this regard, the pupil near reflex, one component of the triadic accommodative response to a visual plane shift in-depth, may play a key role. Such reflex does not necessitate any requirement of skeletal muscles, and thus may be preserved in diseases affecting somatic motoneurons, such as the amyotrophic lateral sclerosis. On this basis, the pupillary accommodative response, i.e. the pupil constriction in response to a far-to-near visual shift in depth, can constitute an effective communication tool, bypassing the well-known limitations of canonical eye-trackers, which require recruitment of skeletal muscles. This paper introduces e-Pupil: a low-cost, stand-alone, portable and smart device exploiting the pupillary accommodative response as a communication tool. The Internet of Things plays a crucial role in our device, guaranteeing portability, accessibility and usability, as well as several remote functionalities. We propose two different routes to communicate with the external world, both built upon the identification of the pupil constriction event in response to a voluntary far-to-near visual shift. The experimental validation proves the reliability of the system as well as its intrinsic simplicity of use.

There are many cases in which the separation of different sources from single channel recordings is important, for example, in fluorescence spectral overlap compensation, electrical impedance signaling, intramuscular electromyogram decomposition or in the case of spike sorting of neuron potentials from microelectrode arrays (MEA). Focusing on the latter, the problem can be faced by identifying spikes emerging from the background and clustering into different groups, indicating the activity of different neurons. Problems are found when more spikes are superimposed in overlapped waveforms. We discuss the application of Biogeography-Based Optimization (BBO) to resolve this specific problem. Our algorithm is compared with three spike-sorting methods (SpyKING Circus, Common Basis Pursuit and Klusta), showing statistically better performance (in terms of F1 score, True Positive Rate—TPR and Positive Predictive Value—PPV) in resolving overlaps in realistic, simulated data. Specifically, BBO showed median F1, TPR and PPV of 100%, 100% and about 75%, respectively, considering a simulated noise with the same spectral density as the experimental one and a similar power with highly statistically significant improvements of at least two performance indexes over each of the other three tested algorithms.

Sustained attention is pivotal for tasks like studying and working for which focus and low distractions are necessary for peak productivity. This study explores the effectiveness of adaptive transcranial direct current stimulation (tDCS) in either the frontal or parietal region to enhance sustained attention. The research involved ten healthy university students performing the Continuous Performance Task-AX (AX-CPT) while receiving either frontal or parietal tDCS. The study comprised three phases. First, we acquired the electroencephalography (EEG) signal to identify the most suitable metrics related to attention states. Among different spectral and complexity metrics computed on 3 s epochs of EEG, the Fuzzy Entropy and Multiscale Sample Entropy Index of frontal channels were selected. Secondly, we assessed how tDCS at a fixed 1.0 mA current affects attentional performance. Finally, a real-time experiment involving continuous metric monitoring allowed personalized dynamic optimization of the current amplitude and stimulation site (frontal or parietal). The findings reveal statistically significant improvements in mean accuracy (94.04 vs. 90.82%) and reaction times (262.93 vs. 302.03 ms) with the adaptive tDCS compared to a non-stimulation condition. Average reaction times were statistically shorter during adaptive stimulation compared to a fixed current amplitude condition (262.93 vs. 283.56 ms), while mean accuracy stayed similar (94.04 vs. 93.36%, improvement not statistically significant). Despite the limited number of subjects, this work points out the promising potential of adaptive tDCS as a tailored treatment for enhancing sustained attention.

Ultrasound (US) scans of inferior vena cava (IVC) are widely adopted by healthcare providers to assess patients’ volume status. Unfortunately, this technique is extremely operator dependent. Recently, new techniques have been introduced to extract stable and objective information from US images by automatic IVC edge tracking. However, these methods require prior interaction with the operator, which leads to a waste of time and still makes the technique partially subjective. In this paper, two deep learning methods, YOLO (You only look once) v4 and YOLO v4 tiny networks, commonly used for fast object detection, are applied to identify the location of the IVC and to recognise the either long or short axis view of the US scan. The output of these algorithms can be used to remove operator dependency, to reduce the time required to start an IVC analysis, and to automatically recover the vein if it is lost for a few frames during acquisition. The two networks were trained with frames extracted from 18 subjects, labeled by 4 operators. In addition, they were also trained on a linear combination of two frames that extracted information on both tissue anatomy and movement. We observed similar accuracy of the two models in preliminary tests on the entire dataset, so that YOLO v4 tiny (showing much lower computational cost) was selected for additional cross-validation in which training and test frames were taken from different subjects. The classification accuracy was approximately 88% when using original frames, but it reached 95% when pairs of frames were processed to also include information on tissue movements, indicating the importance of accounting for tissue motion to improve the accuracy of our IVC detector.