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Adenovirus vector-based genetic vaccines have emerged as a powerful strategy against the SARS-CoV-2 health crisis. This success is not unexpected because adenoviruses combine many desirable features of a genetic vaccine. They are highly immunogenic and have a low and well characterized pathogenic profile paired with technological approachability. Ongoing efforts to improve adenovirus-vaccine vectors include the use of rare serotypes and non-human adenoviruses. In this review, we focus on the viral capsid and how the choice of genotypes influences the uptake and subsequent subcellular sorting. We describe how understanding capsid properties, such as stability during the entry process, can change the fate of the entering particles and how this translates into differences in immunity outcomes. We discuss in detail how mutating the membrane lytic capsid protein VI affects species C viruses’ post-entry sorting and briefly discuss if such approaches could have a wider implication in vaccine and/or vector development.
Coralie Daussy; Noémie Pied; Harald Wodrich. Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties. Viruses 2021, 13, 1221 .
AMA StyleCoralie Daussy, Noémie Pied, Harald Wodrich. Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties. Viruses. 2021; 13 (7):1221.
Chicago/Turabian StyleCoralie Daussy; Noémie Pied; Harald Wodrich. 2021. "Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties." Viruses 13, no. 7: 1221.
Incoming adenoviruses seize control of cytosolic transport mechanisms to relocate their genome from the cell periphery to specialized sites in the nucleoplasm. The nucleus is the site for viral gene expression, genome replication and the production of progeny for the next round of infection. By taking control of the cell, adenoviruses also suppress cell autonomous immunity responses. To succeed in their production cycle, adenoviruses rely on well‐coordinated steps, facilitated by interactions between viral proteins and cellular factors. Interactions between virus and host can impose remarkable morphological changes in the infected cell. Imaging adenoviruses has tremendously influenced how we delineate individual steps in the viral life cycle, because it allowed the development of specific optical markers to label these morphological changes in space and time. As technology advances, innovative imaging techniques and novel tools for specimen labeling keeps uncovering previously unseen facets of adenovirus biology emphasizing why imaging adenoviruses is as attractive today as it was in the past. This review will summarize past achievements and present developments in adenovirus imaging centered on fluorescence microscopy approaches.
Noémie Pied; Harald Wodrich. Imaging the adenovirus infection cycle. FEBS Letters 2019, 593, 3419 -3448.
AMA StyleNoémie Pied, Harald Wodrich. Imaging the adenovirus infection cycle. FEBS Letters. 2019; 593 (24):3419-3448.
Chicago/Turabian StyleNoémie Pied; Harald Wodrich. 2019. "Imaging the adenovirus infection cycle." FEBS Letters 593, no. 24: 3419-3448.