SUMMARY: Successful crop production depends initially on the availability of high-quality seed. By 2050 global climate change will have influenced crop yields, but will these changes affect seed quality? The present review examines the effects of elevated carbon dioxide (CO₂) and temperature during seed production on three seed quality components: seed mass, germination and seed vigour.In response to elevated CO₂, seed mass has been reported to both increase and decrease in C₃plants, but not change in C₄plants. Increases are greater in legumes than non-legumes, and there is considerable variation among species. Seed mass increases may result in a decrease of seed nitrogen (N) concentration in non-legumes. Increasing temperature may decrease seed mass because of an accelerated growth rate and reduced seed filling duration, but lower seed mass does not necessarily reduce seed germination or vigour.Like seed mass, reported seed germination responses to elevated CO₂have been variable. The reported changes in seed C/N ratio can decrease seed protein content which may eventually lead to reduced viability. Conversely, increased ethylene production may stimulate germination in some species. High-temperature stress before developing seeds reach physiological maturity (PM) can reduce germination by inhibiting the ability of the plant to supply the assimilates necessary to synthesize the storage compounds required for germination.Nothing is known concerning the effects of elevated CO₂on seed vigour. However, seed vigour can be reduced by high-temperature stress both before and after PM. High temperatures induce or increase the physiological deterioration of seeds. Limited evidence suggests that only short periods of high-temperature stress at critical seed development stages are required to reduce seed vigour, but further research is required.The predicted environmental changes will lead to losses of seed quality, particularly for seed vigour and possibly germination. The seed industry will need to consider management changes to minimize the risk of this occurring.

Soil-borne plant pathogens such as Rhizoctonia solani (Kuhn), Pythium ultimum (Trow) and Sclerotinia trifoliorum (Eriks) can reduce grass and forage legume establishment. The potential for biocontrol of these pathogens by Trichoderma fungi was evaluated. Following dual culture assays, nine Trichoderma isolates (five of Trichoderma atroviride and one each of Trichoderma hamatum, Trichoderma koningiopsis, Trichoderma viride and Trichoderma virens) were chosen for assessment in pot experiments. In the presence of R. solani, perennial ryegrass (Lolium perenne L.) emergence was increased by 60–150% by two isolates of T. atroviride and by 35–212% by the isolate of T. virens, with the increase depending on growing medium and amount of pathogen inoculum. Red clover (Trifolium pratense L.) emergence in the presence of S. trifoliorum was significantly increased by two T. atroviride isolates and the T. hamatum isolate. In the presence of P. ultimum, white clover (Trifolium repens L.) emergence was increased by 25–42% by one isolate of T. atroviride and the T. hamatum isolate. However, for all three pasture species, some Trichoderma isolates reduced seedling emergence. Seedling growth (shoot and root fresh weight/plant) of the three pasture species was significantly increased by one or more T. atroviride isolates. On the basis of these results for both disease reduction and growth promotion, four T. atroviride isolates were selected for field assessment as biocontrol agents of soil-borne pathogens of pasture species.

Food security depends on seed security and the international seed industry must be able to continue to deliver the quantities of quality seed required for this purpose. Abiotic stress resulting from climate change, particularly elevated temperature and water stress, will reduce seed yield and quality. Options for the seed industry to adapt to climate change include moving sites for seed production, changing sowing date, and the development of cultivars with traits which allow them to adapt to climate change conditions. However, the ability of seed growers to make these changes is directly linked to the seed system. In the formal seed system operating in developed countries, implementation will be reasonably straight forward. In the informal system operating in developing countries, the current seed production challenges including supply failing to meet demand and poor seed quality will increase with changing climates.

In eukaryotic systems, membrane-bound NADPH oxidases (Nox) generate reactive oxygen species (ROS) as a part of normal physiological functions. In the soil-borne mycoparasitic and plant facultative symbiont Trichoderma atroviride, Nox1 and the regulator NoxR are involved in differentiation induced by mechanical damage, while the role of Nox2 has not been determined. The knock-out strains Δnox1, ΔnoxR and Δnox2 were compared to the parental strain (WT) in their ability to grow and conidiate under a series of stress conditions (osmotic, oxidative, membrane, and cell-wall stresses). All three genes were differentially involved in the stress-response phenotypes. In addition, several interactive experiments with biotic factors (plant seedlings and other fungi) were performed comparing the mutant phenotypes with the WT, which was used as the reference strain. Δnox1 and ΔnoxR significantly reduced the antagonistic activity of T. atroviride against Rhizoctonia solani and Sclerotinia sclerotiorum in direct confrontation assays, but Δnox2 showed similar activity to the WT. The Δnox1, ΔnoxR, and Δnox2 mutants showed quantitative differences in the emission of several volatile organic compounds (VOCs). The effects of a blend of these volatiles on plant-growth promotion of Arabidopsis thaliana seedlings were determined in closed-chamber experiments. The increase in root and shoot biomass induced by T. atroviride VOCs was significantly lowered by ΔnoxR and Δnox1, but not by Δnox2. In terms of fungistatic activity at a distance, Δnox2 had a significant reduction in this trait against R. solani and S. sclerotiorum, while fungistasis was highly increased by ΔnoxR and Δnox1. Identification and quantification of individual VOCs in the blends emitted by the strains was performed by GC-MS and the patterns of variation observed for individual volatiles, such as 6-Pentyl-2H-pyran-2-one (6PP-1) and (E)-6-Pent-1-enylpyran-2-one (6PP-2) were consistent with their negative effects in plant-growth promotion and positive effects in fungistasis at a distance. Nox1 and NoxR appear to have a ubiquitous regulatory role of in a variety of developmental and interactive processes in T. atroviride either as positive or negative modulators. Nox2 may also have a role in regulating production of VOCs with fungistatic activity.