Our understanding of the post-transcriptional mechanisms involved in follicular atresia is limited; however, an important development has been made in understanding the biological regulatory networks responsible for mediating follicular atresia. MicroRNAs have come to be seen as a key regulatory actor in determining cell fate in a wide range of tissues in normal and pathological processes. Profiling studies of miRNAs during follicular atresia and development have identified several putative miRNAs enriched in apoptosis signaling pathways. Subsequent in vitro and/or in vivo studies of granulosa cells have elucidated the functional role of some miRNAs along with their molecular pathways. In particular, the regulatory roles of some miRNAs have been consistently observed during studies of follicular cellular apoptosis. Continued work should gradually lead to better understanding of the role of miRNAs in this field. Ultimately, we expect this understanding will have substantial benefits for fertility management at both the in vivo or/and in vitro levels. The stable nature of miRNA holds remarkable promise in clinical use as a diagnostic tool and in reproductive medicine to solve the ever-increasing fertility problem. In this review, we summarize current knowledge of the involvement of miRNAs in follicular atresia, discuss the challenges for further work and pinpoint areas for future research.

Campylobacter is a fastidious organism that is capable of surviving in wide range of environments and potentially can cause serious infection in human and animal which can commonly available in the different sources of water used for daily purpose. This research was conducted to figure out the prevalence of the thermophilic Campylobacter in the water used in various slaughter houses of Kathmandu and Ruphendehi district of Nepal. So a cross sectional study was conducted and 200 water samples (100 for each district) was collected aseptically and submitted to Bacteriological Unit for the confirmation. Isolation and identification of Campylobacter was being done as described by OIE Terrestrial Manual, 2004, Chapter 2.8.10. Laboratory finding was done to confirm the positive cases. The study revealed the prevalence status of Campylobacter in water used in the slaughter house of Ruphendehi district is 12% and Kathmandu Valley is 0.00%. Thus, Ruphendehi has comparatively more prevalence of Campylobacter than Kathmandu. The water samples tested were all from actual or potential water sources for the use in slaughter house, so there are clear implications for the transmission of Campylobacter spp. to human and animal suggesting further expanded research is required in this area. Int. J. Appl. Sci. Biotechnol. Vol 7(1): 75-80

Japanese Encephalitis (JE) a vector borne zoonoitc disease caused by arbovirus of Flavivirus and transmitted by Culex tritaeniorhynchus additionally pig acts as amplifying host for the virus. A total 115 swine serum samples 100 pig farmers from each district were tested against Japanese encephalitis virus (JEV) infection. The collected samples were analyzed by using “Porcine Encephalitis B Virus Antibody Rapid Test Kit”. Among them 17% samples were positive for JE while 83% samples were negative for JE. Total 18% were positive for JE in Morang district while 115.4% were positive for Kathmandu district. There was no significant difference in prevalence of JE in these two districts (p>0.05). In Kathmandu, the prevalence was 15.4% while in Morang it was slightly higher, 18%. 9.5% younger pigs of 3-9 months of age were positive for JE and 20.6% of age above 9 months were positive of JE but were no significant (p>0.05). Similarly, 17.9% female pigs and 14.6% male pigs were positive for JE however difference was not significantly different. In case of breed, no association of pigs with seropositivity (p>0.05), 7 (13.5%) out of 52 local breed pigs and 12 (19.1%) out of 63 were JE positive. The prevalence rate of JEV was higher in Morang district among study area which might be due to several factors like lack of education in pig farmers, non vaccinated pig population and lack of awareness regarding risk factor of JEV. Int. J. Appl. Sci. Biotechnol. Vol 6(4): 373-378