A number of plants used in folk medicine in Thailand and Eastern Asia are attracting interest due to the high bioactivities of their extracts. The aim of this study was to screen the edible leaf extracts of 20 plants found in Thailand and investigate the potential neuroprotective effects of the most bioactive sample. The total phenol and flavonoid content and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity were determined for all 20 leaf extracts. Based on these assays, Glochidion littorale leaf extract (GLE), which showed a high value in all tested parameters, was used in further experiments to evaluate its effects on neurodegeneration in Caenorhabditis elegans. GLE treatment ameliorated H₂O₂-induced oxidative stress by attenuating the accumulation of reactive oxygen species and protected the worms against 1-methyl-4-phenylpyridinium-induced neurodegeneration. The neuroprotective effects observed may be associated with the activation of the transcription factor DAF-16. The characterization of this extract by LC-MS identified several phenolic compounds, including myricetin, coumestrin, chlorogenic acid, and hesperidin, which may play a key role in neuroprotection. This study reports the novel neuroprotective activity of GLE, which may be used to develop treatments for neurodegenerative diseases such as Parkinson’s syndrome.

In many districts of Benin, the use of wastewater in urban agriculture is becoming more and more widespread. This activity around wastewater discharges potentially poses health risks to populations. As water is one of the main sources of food contamination in developing countries, the main objective of this study, oriented towards the assessment of the bacterial load, was to search for Salmonella which are pathogenic to humans in irrigation water as well as in some market gardening products consumed in Parakou district. The study was carried out on the market gardening perimeter of the slaughterhouse site located near the international marketArzèkè, where market gardeners exclusively use surface water from the mixture of groundwater and runoff from installed collectors. At the end of this study, the results from the observation of the different colonies, followed by biochemical tests for the detection and differentiation of Salmonella, allowed us to detect the presence of Salmonella in the different samples ranging from 50% to 80%. The presence of Fecal coliforms and Escherichia coli not only in water but also in market garden products was also confirmed. These results might partly explain the frequency of salmonellosis in the study area. Keywords: Salmonella, microbiological tests, biochemical tests.