Nowadays, plastics represent growing concern in aquatic habitats, harming more and more both ecosystem and human health. Since rivers are the main carriers of plastics to the sea, some studies were conducted on the transport of overall small plastics, but observations on big plastics (i.e., macroplastics) in rivers are poorly reported in the literature. Although most studies focused on plastic transport in the lower part of the rivers, research on the upper and middle zones are completely lacking. In this regard, this could influence the transport of plastics to the sea, with insights into the source and origin of macroplastics. Here, we aimed at overviewing macroplastic distribution along rivers, emphasizing on the global riverine plastic hotspot areas. First, 1) we analyzed and discussed the bibliometric analysis on macroplastics in rivers providing a framework for plastic pollution management. second, 2) we provided geographical insights into macroplastics by mapping global riverine plastic hotspot areas. Then, 3) we analyzed the main factors affecting macroplastic distribution and accumulation in rivers. Furthermore, 4) we assessed crucial gaps in riverine macroplastic accumulation, highlighting the importance of a plastic gradient in the horizontal transport along the entire river course (i.e., three river zones). Then, 5) we highlighted the lack of standardization regarding macroplastic size, concentration, and polymers that does not allow valuable comparisons among studies. At the end, 6) we concluded by providing future perspectives and conclusions on macroplastic distribution and accumulation in rivers worldwide. Our results might provide new insights into a comprehensive framework of macroplastic distribution along global rivers, suggesting the river rod approach as a way for future monitoring as all the complete course of a river plays a pivotal role in accumulating macroplastics.

Currently, natural and urban ecosystems are affected by different types of atmospheric deposition, which can compromise the balance of the environment. Plastic pollution represents one of the major threats for biota, including lichens. Epiphytic lichens have value as bioindicators of environmental pollution, climate change, and anthropic impacts. In this study, we aim to investigate the lichen bioaccumulation of airborne microplastics along an anthropogenic pollution gradient. We sampled lichens from the Genera Cladonia and Xanthoria to highlight the effectiveness of lichens as tools for passive biomonitoring of microplastics. We chose three sites, a "natural site" in Altipiani di Arcinazzo, a "protected site" in Castelporziano Presidential estate and an "urban site" in the centre of Rome. Overall, we sampled 90 lichens, observed for external plastic entrapment, melt in oxygen peroxide and analysed for plastic entrapment. To validate the method, we calculated recovery rates of microplastics in lichen. Particularly, 253 MPs particles were detected across the 90 lichen samples: 97 % were fibers, and 3 % were fragments. A gradient in the number of microplastic fibers across the sites emerged, with increasing accumulation of microplastics from the natural site (n = 58) to the urban site (n = 116), with a direct relationship between the length and abundance of airborne microplastic fibers. Moreover, we detected the first evidences of airborne mesoplastics entrapped by lichens. On average, the natural site experienced the shortest fibre length and the centre of Rome the longest. No differences in microplastics accumulation emerged from the two genera. Our results indicated that lichens can effectively be used for passive biomonitoring of microplastic deposition. In this scenario, the role of lichens in entrapping microplastics and protecting pristine areas must be investigated. Furthermore, considering the impact that airborne microplastics can have on human health and the effectiveness of lichens as airborne microplastic bioindicators, their use is encouraged.

Anthropogenic plastic litter is widespread in all environments, with particular emphasis on aquatic habitats. Specifically, although freshwater mammals are important as they are at the top of food web, research mainly focus on marine animals, while only few studies have been carried out on freshwater mammals. The main gap is that microplastics (MP) are completely understudied in freshwater mammals. Here, we reported the first evidence of the presence of anthropogenic particles (including MP) in coypu (Myocastor coypus)’ faeces. Coypu is a rodent mammal inhabiting rivers and wetland areas, and we discussed our preliminary data suggesting the use of these tracks as possible future bioindicator of MP pollution in wetlands and freshwaters. We collected 30 coypu’s faeces in “Torre Flavia wetland” nature reserve. Then, in laboratory, faeces were digested in 30 ml hydrogen peroxide (30%) for a week a 20 °C and analysed under stereoscope. All the suspected found MP were isolated in a petri dish, using FT-IR analysis to confirm the polymers. Overall, we recorded 444 natural and anthropogenic particles with most of items being fibres. FT-IR analysis of the 10% of the particles recovered revealed that 72% of them was not MP (mainly, polyethylene, polyethylene terephthalate, and polyamide). Also, the number of anthropogenic particles is not correlated with the faecal weight. Given that alien species, such as coypu, are widespread species, our results might have a great importance as these species and MP in faecal tracks may be used as undirect proxy of environmental bioavailability of MP pollution.

Anthropogenic litter accumulates along coasts worldwide. In addition to the flowing litter load, wind, sea currents, geomorphology and vegetation determine the distribution of litter trapped on the sandy coasts. Although some studies highlighted the role of dune plants in trapping marine litter, little is known about their efficiency as sinks and about the small-scale spatial distribution of litter across the dune area. Here, we explore these gaps by analysing six plant species widespread in Mediterranean coastal habitats, namely Echinophora spinosa, Limbarda crithmoides, Anthemis maritima, Pancratium maritimum, Thinopyrum junceum, and Salsola kali. The present study analyses for the first time the capture of litter by dune vegetation at a multi-species level, considering their morphological structure. Data on plastic accumulation on dune plants were compared with unvegetated control plots located at embryo-dune and foredune belts. We found that dunal plants mainly entrapped macrolitter (> 0.5 cm). Particularly, E. spinosa, L. crithmoides, A. maritima and P. maritimum mostly accumulated litter in the embryo dune while T. junceum and S. kali entrapped more in the foredune area. Moreover, beach litter was mainly blocked at the edge of the plant patches rather than in the core, highlighting the 'Plant-edge litter effect'. As A. maritima and S. kali entrapped respectively more litter in embryo and foredune habitats, these species could be used to monitor and recollect litter. In this light, our findings provide further insight into the role of dune plants in the beach litter dynamics, suppling useful information for beach clean-up actions.