The cytoskeleton is involved in several biological processes, including adhesion, motility, and intracellular transport. Alterations in the cytoskeletal components (actin filaments, intermediate filaments, and microtubules) are strictly correlated to several diseases, such as cancer. Furthermore, alterations in the cytoskeletal structure can lead to anomalies in cells’ properties and increase their invasiveness. This review aims to analyse several studies which have examined the alteration of the cell cytoskeleton induced by ionizing radiations. In particular, the radiation effects on the actin cytoskeleton, cell adhesion, and migration have been considered to gain a deeper knowledge of the biophysical properties of the cell. In fact, the results found in the analysed works can not only aid in developing new diagnostic tools but also improve the current cancer treatments.

The structural and mechanical properties of the microenvironmental context have a profound impact on cancer cell motility, tumor invasion, and metastasis formation. In fact, cells react to their mechanical environment modulating their adhesion, cytoskeleton organization, changes of shape, and, consequently, the dynamics of their motility. In order to elucidate the role of extracellular matrix stiffness as a driving force in cancer cell motility/invasion and the effects of ionizing radiations on these processes, we evaluated adhesion and migration as biophysical properties of two different mammary cell lines, over a range of pathophysiological stiffness (1–13 kPa) in a control condition and after the exposure to two different X-ray doses (2 and 10 Gy, photon beams). We concluded that the microenvironment mimicking the normal mechanics of healthy tissue has a radioprotective role on both cell lines, preventing cell motility and invasion. Supraphysiological extracellular matrix stiffness promoted tumor cell motility instead, but also had a normalizing effect on the response to radiation of tumor cells, lowering their migratory capability. This work lays the foundation for exploiting the extracellular matrix-mediated mechanism underlying the response of healthy and tumor cells to radiation treatments and opens new frontiers in the diagnostic and therapeutic use of radiotherapy.

The physical–chemical properties of water are closely linked to the geological nature of the site where they are located. This aspect becomes even more interesting when analyzing the natural radionuclides in the drinking water of a volcanic territory such as Campania in southern Italy. This study concerned the measurement of activity concentration of gross alpha and beta, radon, and tritium to evaluate their biological impact. The measurements were carried out using alpha spectrometry for alpha emitters, proportional counter for beta emitters, the electret system for radon in water, and finally liquid scintillation for the measurement of tritium concentration. The biological impact was assessed considering the indicative dose, if applicable, and the effective annual dose of radon. Although the results show that the values are below international and national references, the radiological characterization of drinking water is of fundamental importance to optimize the radiation protection of the population.

The Sassi of Matera (Basilicata region, southern Italy) is a peculiar site, but not unique in Italy, from a landscape point of view. Most of the buildings are excavated in rocks of calcarenite origin and used as homes and accommodation facilities. For this reason, the contribution of gamma radiation to the population due to this building material has been investigated for the first time. Even though the type of rock is not mentioned among the construction materials indicated in Italian Legislative Decree 101/2020 to be subjected to radiological characterization, the methodology indicated in the decree was applied, comparing the values with those present in the literature. The content of ²²⁶Ra, ²³²Th and ⁴⁰K was then investigated with gamma spectroscopy, and the gamma index was calculated. The results obtained showed a low level of activity concentration of natural radionuclides in this type of stone, confirming the radiological safety. This result can also support the recovery and restoration initiatives for these buildings to conserve the architectural and landscape heritage of this village.